LOCUS       NM_001408940            3122 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus T cell activation inhibitor, mitochondrial (Tcaim),
            transcript variant 8, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001408940 XM_006512173
VERSION     NM_001408940.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 3122)
  AUTHORS   Vogel,S.Z., Schlickeiser,S., Jurchott,K., Akyuez,L., Schumann,J.,
            Appelt,C., Vogt,K., Schroder,M., Vaeth,M., Berberich-Siebelt,F.,
            Lutz,M.B., Grutz,G. and Sawitzki,B.
  TITLE     TCAIM decreases T cell priming capacity of dendritic cells by
            inhibiting TLR-induced Ca2+ influx and IL-2 production
  JOURNAL   J Immunol 194 (7), 3136-3146 (2015)
   PUBMED   25750433
  REMARK    GeneRIF: TCAIM decreases T cell priming capacity of dendritic cells
            by inhibiting TLR-induced Ca2+ influx and IL-2 production.
REFERENCE   4  (bases 1 to 3122)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 3122)
  AUTHORS   Keeren,K., Friedrich,M., Gebuhr,I., Philipp,S., Sabat,R.,
            Sterry,W., Brandt,C., Meisel,C., Grutz,G., Volk,H.D. and
            Sawitzki,B.
  TITLE     Expression of tolerance associated gene-1, a mitochondrial protein
            inhibiting T cell activation, can be used to predict response to
            immune modulating therapies
  JOURNAL   J Immunol 183 (6), 4077-4087 (2009)
   PUBMED   19684086
REFERENCE   6  (bases 1 to 3122)
  AUTHORS   Sawitzki,B., Bushell,A., Steger,U., Jones,N., Risch,K., Siepert,A.,
            Lehmann,M., Schmitt-Knosalla,I., Vogt,K., Gebuhr,I., Wood,K. and
            Volk,H.D.
  TITLE     Identification of gene markers for the prediction of allograft
            rejection or permanent acceptance
  JOURNAL   Am J Transplant 7 (5), 1091-1102 (2007)
   PUBMED   17456197
  REMARK    GeneRIF: Downregulation of TOAG-1 and alpha-1,2-mannosidase
            expression occurred in graft infiltrating cells and expression of
            both genes was also downregulated after T-cell activation in vitro.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125374.4.
            
            On Jul 5, 2022 this sequence version replaced XM_006512173.5.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR12282455.27312081.1,
                                           SRR17253012.8822447.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-148               AC125374.4         151419-151566       c
            149-218             AC125374.4         148271-148340       c
            219-431             AC125374.4         145214-145426       c
            432-588             AC125374.4         142519-142675       c
            589-841             AC125374.4         138081-138333       c
            842-964             AC125374.4         132522-132644       c
            965-1062            AC125374.4         130817-130914       c
            1063-1154           AC125374.4         130169-130260       c
            1155-1393           AC125374.4         125249-125487       c
            1394-1525           AC125374.4         123450-123581       c
            1526-3122           AC125374.4         120775-122371       c
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 73.14 cM"
     gene            1..3122
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /note="T cell activation inhibitor, mitochondrial"
                     /db_xref="GeneID:382117"
                     /db_xref="MGI:MGI:1196217"
     exon            1..148
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /inference="alignment:Splign:2.1.0"
     exon            149..218
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             156..1766
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /note="isoform 3 is encoded by transcript variant 8;
                     tolerance-associated gene 1; tolerance associated gene-1
                     protein"
                     /codon_start=1
                     /product="T-cell activation inhibitor, mitochondrial
                     isoform 3"
                     /protein_id="NP_001395869.1"
                     /db_xref="GeneID:382117"
                     /db_xref="MGI:MGI:1196217"
                     /translation="MDAWKLACLFGNVLPLEAFEEVGCTSYMVMYGGWMDVTESNPCV
                     SDRFGLRKVLPHWLHYSRALSGAEAINALRPFYFAVHPDFFGQHPREREVNENSLKRL
                     SVYLENLQKPGFKSLKPTQLTFYIREKTAQNSSEGQEPISTTGFRAVRFTLHSSDLLS
                     TVLYILNSCSLPVEHVQSLNTNVHSQPLKEATGMPDRPIKWHRSYYSFTGFKDPDEDL
                     THVSRVETTLTSWLGSNGKGAVKKLRNSLPLRKELDRLKNELSELLQLSDIRWQRGWG
                     VAHRCSQLHSLSRLAQQNPGPLQNVKGCTVVFTDRSGMSALGHVMLGTMDVHHHWTRL
                     FESLPSYFDLQRRMSALEAQISNLLGGIQVVYIEELQPALTLDQYYSLLHTFYNQLLR
                     SRVPPHPHPQSLSGLQMILSSDRYAPSLHELGHFDIPALSDPASLQSFMRTKAQQARE
                     NMTRREKLKVMENELIQASTKQFSLEKLYKEPSISSRQMVDCCKRLLEQSLPYLHGMH
                     LCVSHFYSVMQDGDLCIPWNWKKGEAMK"
     exon            219..431
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /inference="alignment:Splign:2.1.0"
     exon            432..588
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /inference="alignment:Splign:2.1.0"
     exon            589..841
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /inference="alignment:Splign:2.1.0"
     exon            842..964
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1062
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1154
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1393
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1525
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..3122
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3098..3103
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /note="hexamer: AATAAA"
     polyA_site      3122
                     /gene="Tcaim"
                     /gene_synonym="D9Ertd402e; Gm1129; TOAG-1"
                     /note="major polyA site"
ORIGIN      
        1 ggccccgccc ctgcctcccg ctactccttt acctttgctt atcgtcctcc cacctgcctg
       61 ccaatcatct ttctccctca gacctagtcg gccggaagaa ccggcttgtg tctcccgaag
      121 ctgccttcgg aaacccgcag atagcggggc aattaatgga tgcttggaag ttggcgtgct
      181 tattcggaaa tgttttgcca cttgaggcct ttgaggaggt tggctgcacc tcctacatgg
      241 tgatgtatgg tggatggatg gatgtcacag agagtaatcc ctgtgtctct gataggttcg
      301 gcctgaggaa ggtcctccct cattggcttc actactctcg agctttatca ggggcagaag
      361 ccatcaacgc cttgaggcct ttctactttg cagtgcatcc tgatttcttt ggacagcacc
      421 ccagggaaag ggaagtcaat gaaaattctc ttaagagatt aagtgtctat ttagaaaatc
      481 tccagaaacc aggcttcaaa tctctgaaac caactcagct tacattttat ataagagaaa
      541 aaacagccca gaattcctct gaaggccagg agcctatcag tactaccgga ttccgagcag
      601 tcagatttac tttgcacagc agcgatctgc taagcacagt attgtatatt ctcaactcct
      661 gcagtttgcc tgttgaacac gtccaaagct tgaacactaa tgtgcattcc cagcctctca
      721 aggaagctac agggatgcct gacagaccca tcaaatggca taggtcctat tattccttta
      781 ctgggttcaa ggaccctgat gaagacctca cacacgtctc aagagtggaa acaaccctaa
      841 cgtcctggtt aggtagcaat gggaaaggtg ccgtgaagaa gctgaggaac agtctgccac
      901 ttcggaagga gctggatcgc ctgaagaatg agctgtctga gcttctgcag ctttcagaca
      961 tcaggtggca gagaggctgg ggagttgccc atcgctgcag ccagctgcac agtctcagcc
     1021 gcctagcaca gcagaatccg gggccacttc agaatgtgaa aggatgcacg gttgtattca
     1081 cagaccgctc cggtatgagt gcactgggcc atgtgatgct gggaaccatg gatgtccacc
     1141 accactggac aaggcttttt gaaagcttgc caagttattt tgaccttcag aggagaatgt
     1201 cagccttaga agcccaaata agcaatcttc tagggggaat ccaagtggtt tatatcgaag
     1261 agttgcagcc agcactcaca cttgatcagt actactctct cctccacacc ttctacaacc
     1321 agctgctgag aagcagggtg ccgccccacc cccaccccca gagtctgagc ggcttgcaga
     1381 tgatccttag cagtgacagg tatgcgccaa gcttgcatga actcgggcat tttgatatcc
     1441 cagccctctc agatccagca agcctgcagt ccttcatgag aaccaaagcc cagcaggcga
     1501 gagaaaacat gactagaagg gagaagttaa aagttatgga aaatgaattg atacaggctt
     1561 caacaaagca gttttctctg gagaagttgt ataaagagcc cagcatttct agcagacaga
     1621 tggtggattg ctgtaagaga cttcttgaac agtcgctgcc atatctgcat gggatgcacc
     1681 tctgcgtctc acatttctat tctgttatgc aagatggaga cctgtgcatc ccgtggaact
     1741 ggaagaaagg agaagccatg aagtaacaca gatatctgtt ttattttctg aagaaatgaa
     1801 aaactgttga atttatttaa attcagtttg atataacatt agtatttaca tgttagaaca
     1861 aagtgtctaa ctgctgctat aaaagtgggg agggttttct ttttagtgat tttatgctaa
     1921 gaaacttgct tttaaaaggt tttatcccct agacttatga tgctgatggc taggcaaaag
     1981 atactagaag aaaaaaaact attttaaaga tatgcaatag aaggttctgt aagagtcata
     2041 tatttttata gaataaatgt gagtaatgta agttggtctc gctcagtata gctctttagg
     2101 actgggactc catttttaca tgcaacacag tttaatgtgc accttcatag ttacatcgta
     2161 tacacacaca cactttgtag ccagtaatag acggttattc aagacagatg actggcaagt
     2221 agcaaaagtt aaataaccaa attaaaagtt aaataaccaa attaaaaatt aaataaccaa
     2281 attttgctta agagaaagat tttttaaaac cctattgaat atatactatt aacattgtct
     2341 gatacaagtt tactaaagca tcagtaacag agaacttaaa tacatgattt cttctaccaa
     2401 ttaaagtatc agtggagcat atacttggtg ggcacaggca tcctcaagat gagtaagatg
     2461 taggattata acttagcctg aagctcctta aaaagctttc ttcacttctt cagctctggc
     2521 ccaggcagag ccctgtgcta tggctcgctc tcaggtgaag catggcctcc tttgtgctca
     2581 gctgtctcct gcacagtgag aggcctgctc tctgttcaga gtagacccac agtacattag
     2641 aacaaaggag atgctaaaat cagtttttca tgttttgggt ttttatgaaa catcattaat
     2701 ttaaacagtt caaatgtatg ttcaaaataa gaaatggctt ataaaaggat tgtgtgtgcc
     2761 attgtggctt ttgacccatg ctaatgtgac aaatgttgct gtaaatactc ttatgaaaca
     2821 gtccctaagt agatttggtg gcacacatct ttaatcccag ctcttgggaa gcagaggcag
     2881 gtggatctct gtgagtttaa ggccagcctg gtctataaag tgagtttcag gacaaccggg
     2941 gatgttaaac acagagtaac cctgtctggg gggtggggcc gtcaaacctg acatttatta
     3001 gaaaattgct tggattgtac cgtgtctaat atcagctaac gtggaatctg ctgtaatgtg
     3061 tgttgtattg gatttgtctc agaactaatt gttacataat aaagcaagcc ttggccacca
     3121 ca
//
