LOCUS       NM_025548               1343 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus tubulin folding cofactor B (Tbcb), transcript variant
            1, mRNA.
ACCESSION   NM_025548
VERSION     NM_025548.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Kuh,G.F., Stockmann,M., Meyer-Ohlendorf,M., Linta,L., Proepper,C.,
            Ludolph,A.C., Bockmann,J., Boeckers,T.M. and Liebau,S.
  TITLE     Tubulin-binding cofactor B is a direct interaction partner of the
            dynactin subunit p150(Glued)
  JOURNAL   Cell Tissue Res 350 (1), 13-26 (2012)
   PUBMED   22777741
  REMARK    GeneRIF: Overexpression of TBCB leads to the decreased localization
            of p150 to the microtubule network that might result in a
            functional modulation of this protein complex.
REFERENCE   3  (bases 1 to 1343)
  AUTHORS   Lo,J.C., Jamsai,D., O'Connor,A.E., Borg,C., Clark,B.J.,
            Whisstock,J.C., Field,M.C., Adams,V., Ishikawa,T., Aitken,R.J.,
            Whittle,B., Goodnow,C.C., Ormandy,C.J. and O'Bryan,M.K.
  TITLE     RAB-like 2 has an essential role in male fertility, sperm
            intra-flagellar transport, and tail assembly
  JOURNAL   PLoS Genet 8 (10), e1002969 (2012)
   PUBMED   23055941
REFERENCE   4  (bases 1 to 1343)
  AUTHORS   Ganay,T., Boizot,A., Burrer,R., Chauvin,J.P. and Bomont,P.
  TITLE     Sensory-motor deficits and neurofilament disorganization in
            gigaxonin-null mice
  JOURNAL   Mol Neurodegener 6, 25 (2011)
   PUBMED   21486449
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1343)
  AUTHORS   Wen,H.L., Lin,Y.T., Ting,C.H., Lin-Chao,S., Li,H. and Hsieh-Li,H.M.
  TITLE     Stathmin, a microtubule-destabilizing protein, is dysregulated in
            spinal muscular atrophy
  JOURNAL   Hum Mol Genet 19 (9), 1766-1778 (2010)
   PUBMED   20176735
REFERENCE   6  (bases 1 to 1343)
  AUTHORS   Lopez-Fanarraga,M., Carranza,G., Bellido,J., Kortazar,D.,
            Villegas,J.C. and Zabala,J.C.
  TITLE     Tubulin cofactor B plays a role in the neuronal growth cone
  JOURNAL   J Neurochem 100 (6), 1680-1687 (2007)
   PUBMED   17217416
REFERENCE   7  (bases 1 to 1343)
  AUTHORS   Kortazar,D., Fanarraga,M.L., Carranza,G., Bellido,J.,
            Villegas,J.C., Avila,J. and Zabala,J.C.
  TITLE     Role of cofactors B (TBCB) and E (TBCE) in tubulin heterodimer
            dissociation
  JOURNAL   Exp Cell Res 313 (3), 425-436 (2007)
   PUBMED   17184771
  REMARK    GeneRIF: We show that TBCB overexpression depolymerizes
            microtubules. We found that this function is based on the ability
            of TBCB to form a binary complex with TBCE that greatly enhances
            the efficiency of this cofactor to dissociate tubulin in vivo and
            in vitro.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC149067.3.
            
            On Feb 8, 2018 this sequence version replaced NM_025548.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC010684.1, AK002316.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386, SAMN00849389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-618               AC149067.3         40279-40896         c
            619-762             AC149067.3         39825-39968         c
            763-859             AC149067.3         36303-36399         c
            860-1051            AC149067.3         35543-35734         c
            1052-1124           AC149067.3         33172-33244         c
            1125-1343           AC149067.3         32828-33046         c
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 17.33 cM"
     gene            1..1343
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /note="tubulin folding cofactor B"
                     /db_xref="GeneID:66411"
                     /db_xref="MGI:MGI:1913661"
     exon            1..618
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     CDS             505..1239
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cytoskeleton-associated protein 1; tubulin-specific
                     chaperone B; cytoskeleton-associated protein CKAPI"
                     /codon_start=1
                     /product="tubulin-folding cofactor B isoform 1"
                     /protein_id="NP_079824.2"
                     /db_xref="CCDS:CCDS21083.1"
                     /db_xref="GeneID:66411"
                     /db_xref="MGI:MGI:1913661"
                     /translation="MEVTGISAPTVMVFISSSLNSFRSEKRYSRSLTIAEFKCKLELV
                     VGSPASCMELELYGADDKFYSKLDQEDALLGSYPVDDGCRIHVIDHSGVRLGEYEDVS
                     KVEKYEISPEAYERRQNTVRSFMKRSKLGPYNEELRAQQEAEAAQRLSEEKAQASAIS
                     VGSRCEVRAPDHSLRRGTVMYVGLTDFKPGYWVGVRYDEPLGKNDGSVNGKRYFECQA
                     KYGAFVKPSAVTVGDFPEEDYGLDEM"
     exon            619..762
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     exon            763..859
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     exon            860..1051
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1124
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1343
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1316..1321
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /note="hexamer: ATTAAA"
     regulatory      1321..1326
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /note="hexamer: ATTAAA"
     polyA_site      1335
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /note="major polyA site"
     polyA_site      1342
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
ORIGIN      
        1 ggatgtctgg ctgacggctt gcgtcgatcc tttgataccc tctgctactc actcccgatt
       61 tcggaacctg cttcccaact ccgagtggaa tggatctagg acaagggctg ggactatgat
      121 gcgcgatgtc atccgcaagg cgctgagcgt gaacgagaga atccgctgct ctcttcttgg
      181 cccaaggcca gtgcacatgc gccgaactta cactcggcgg accgggacaa ctccagccat
      241 tcttcccggc ttcggcctcc tcccgccctc ctctcctgct tcccgtgcac ggaaggcctc
      301 tcgggattgg ttggacgtac ccgagaatcc gcctgtccgt ggagaaagaa cagcagcgat
      361 tggctggcca tgttaggtgg agaacttgca ttggtcagct ccagaacaag gggcgggacc
      421 tatatcccag aagcagcggc ggccactgcg gagccggtgt gagacggctg aaccgcaccg
      481 cgggccactc cggggcgcag cgagatggag gtgacgggga tatcggcgcc caccgtgatg
      541 gttttcatca gcagctccct caacagcttc cgttcggaga agcgatacag tcgtagcctc
      601 accatcgcag agttcaagtg taaactggag ctggtggtgg gcagccctgc ttcctgcatg
      661 gaactggagc tatatggagc agacgacaag ttctacagca agttggatca ggaggatgca
      721 ctgctgggct cctatcctgt ggatgacggc tgtcgcatcc atgtcattga ccacagtggc
      781 gtccggctcg gagagtatga ggatgtgtcc aaagtggaga aatacgagat ctcaccagaa
      841 gcttacgaac ggaggcagaa cacagttcga tccttcatga aacgcagcaa gctaggcccg
      901 tacaatgaag aactccgggc ccagcaagag gcagaggctg cccagcgtct cagtgaggag
      961 aaggcccaag ccagtgccat ctctgtgggg agccgttgtg aggtgcgggc acccgaccac
     1021 tcccttcgca ggggcactgt catgtatgta ggactcacag atttcaagcc aggctactgg
     1081 gttggtgtcc gatatgatga gccgttagga aaaaacgatg gcagtgtgaa tgggaaacgc
     1141 tactttgaat gccaggccaa gtatggtgcc tttgtaaagc catcagctgt caccgtagga
     1201 gatttcccag aggaggacta cgggttggac gagatgtgac tgtacctgga aggaggcccc
     1261 gcccctgaca cagcccctct gaatgcctgt ggcccttttc ctctgagatt tgtgcattaa
     1321 attaaacaga attcagaagg ttg
//
