LOCUS       NM_001360649            1294 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus tubulin folding cofactor B (Tbcb), transcript variant
            3, mRNA.
ACCESSION   NM_001360649
VERSION     NM_001360649.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Kuh,G.F., Stockmann,M., Meyer-Ohlendorf,M., Linta,L., Proepper,C.,
            Ludolph,A.C., Bockmann,J., Boeckers,T.M. and Liebau,S.
  TITLE     Tubulin-binding cofactor B is a direct interaction partner of the
            dynactin subunit p150(Glued)
  JOURNAL   Cell Tissue Res 350 (1), 13-26 (2012)
   PUBMED   22777741
  REMARK    GeneRIF: Overexpression of TBCB leads to the decreased localization
            of p150 to the microtubule network that might result in a
            functional modulation of this protein complex.
REFERENCE   3  (bases 1 to 1294)
  AUTHORS   Lo,J.C., Jamsai,D., O'Connor,A.E., Borg,C., Clark,B.J.,
            Whisstock,J.C., Field,M.C., Adams,V., Ishikawa,T., Aitken,R.J.,
            Whittle,B., Goodnow,C.C., Ormandy,C.J. and O'Bryan,M.K.
  TITLE     RAB-like 2 has an essential role in male fertility, sperm
            intra-flagellar transport, and tail assembly
  JOURNAL   PLoS Genet 8 (10), e1002969 (2012)
   PUBMED   23055941
REFERENCE   4  (bases 1 to 1294)
  AUTHORS   Ganay,T., Boizot,A., Burrer,R., Chauvin,J.P. and Bomont,P.
  TITLE     Sensory-motor deficits and neurofilament disorganization in
            gigaxonin-null mice
  JOURNAL   Mol Neurodegener 6, 25 (2011)
   PUBMED   21486449
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1294)
  AUTHORS   Wen,H.L., Lin,Y.T., Ting,C.H., Lin-Chao,S., Li,H. and Hsieh-Li,H.M.
  TITLE     Stathmin, a microtubule-destabilizing protein, is dysregulated in
            spinal muscular atrophy
  JOURNAL   Hum Mol Genet 19 (9), 1766-1778 (2010)
   PUBMED   20176735
REFERENCE   6  (bases 1 to 1294)
  AUTHORS   Lopez-Fanarraga,M., Carranza,G., Bellido,J., Kortazar,D.,
            Villegas,J.C. and Zabala,J.C.
  TITLE     Tubulin cofactor B plays a role in the neuronal growth cone
  JOURNAL   J Neurochem 100 (6), 1680-1687 (2007)
   PUBMED   17217416
REFERENCE   7  (bases 1 to 1294)
  AUTHORS   Kortazar,D., Fanarraga,M.L., Carranza,G., Bellido,J.,
            Villegas,J.C., Avila,J. and Zabala,J.C.
  TITLE     Role of cofactors B (TBCB) and E (TBCE) in tubulin heterodimer
            dissociation
  JOURNAL   Exp Cell Res 313 (3), 425-436 (2007)
   PUBMED   17184771
  REMARK    GeneRIF: We show that TBCB overexpression depolymerizes
            microtubules. We found that this function is based on the ability
            of TBCB to form a binary complex with TBCE that greatly enhances
            the efficiency of this cofactor to dissociate tubulin in vivo and
            in vitro.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC149067.3 and AA718358.1.
            
            Transcript Variant: This variant (3) uses an alternate splice
            junction compared to variant 1, that causes a frameshift. The
            resulting isoform (3) has a shorter and distinct C-terminus
            compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR3363660.6967.1,
                                           SRR7652917.41331.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386, SAMN00849389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-506               AC149067.3         40391-40896         c
            507-1010            AA718358.1         10-513
            1011-1075           AC149067.3         33172-33236         c
            1076-1294           AC149067.3         32828-33046         c
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 17.33 cM"
     gene            1..1294
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /note="tubulin folding cofactor B"
                     /db_xref="GeneID:66411"
                     /db_xref="MGI:MGI:1913661"
     exon            1..618
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     CDS             505..1059
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /note="isoform 3 is encoded by transcript variant 3;
                     cytoskeleton-associated protein 1; tubulin-specific
                     chaperone B; cytoskeleton-associated protein CKAPI"
                     /codon_start=1
                     /product="tubulin-folding cofactor B isoform 3"
                     /protein_id="NP_001347578.1"
                     /db_xref="GeneID:66411"
                     /db_xref="MGI:MGI:1913661"
                     /translation="MEVTGISAPTVMVFISSSLNSFRSEKRYSRSLTIAEFKCKLELV
                     VGSPASCMELELYGADDKFYSKLDQEDALLGSYPVDDGCRIHVIDHSGVRLGEYEDVS
                     KVEKYEISPEAYERRQNTVRSFMKRSKLGPYNEELRAQQEAEAAQRLSEEKAQASAIS
                     VGSRCEDSQISSQATGLVSDMMSR"
     exon            619..762
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     exon            763..859
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     exon            860..1002
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1075
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1294
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1267..1272
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /note="hexamer: ATTAAA"
     regulatory      1272..1277
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /note="hexamer: ATTAAA"
     polyA_site      1286
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
                     /note="major polyA site"
     polyA_site      1293
                     /gene="Tbcb"
                     /gene_synonym="2410007D12Rik; CG22; Ckap1; CKAPI"
ORIGIN      
        1 ggatgtctgg ctgacggctt gcgtcgatcc tttgataccc tctgctactc actcccgatt
       61 tcggaacctg cttcccaact ccgagtggaa tggatctagg acaagggctg ggactatgat
      121 gcgcgatgtc atccgcaagg cgctgagcgt gaacgagaga atccgctgct ctcttcttgg
      181 cccaaggcca gtgcacatgc gccgaactta cactcggcgg accgggacaa ctccagccat
      241 tcttcccggc ttcggcctcc tcccgccctc ctctcctgct tcccgtgcac ggaaggcctc
      301 tcgggattgg ttggacgtac ccgagaatcc gcctgtccgt ggagaaagaa cagcagcgat
      361 tggctggcca tgttaggtgg agaacttgca ttggtcagct ccagaacaag gggcgggacc
      421 tatatcccag aagcagcggc ggccactgcg gagccggtgt gagacggctg aaccgcaccg
      481 cgggccactc cggggcgcag cgagatggag gtgacgggga tatcggcgcc caccgtgatg
      541 gttttcatca gcagctccct caacagcttc cgttcggaga agcgatacag tcgtagcctc
      601 accatcgcag agttcaagtg taaactggag ctggtggtgg gcagccctgc ttcctgcatg
      661 gaactggagc tatatggagc agacgacaag ttctacagca agttggatca ggaggatgca
      721 ctgctgggct cctatcctgt ggatgacggc tgtcgcatcc atgtcattga ccacagtggc
      781 gtccggctcg gagagtatga ggatgtgtcc aaagtggaga aatacgagat ctcaccagaa
      841 gcttacgaac ggaggcagaa cacagttcga tccttcatga aacgcagcaa gctaggcccg
      901 tacaatgaag aactccgggc ccagcaagag gcagaggctg cccagcgtct cagtgaggag
      961 aaggcccaag ccagtgccat ctctgtgggg agccgttgtg aggactcaca gatttcaagc
     1021 caggctactg ggttggtgtc cgatatgatg agccgttagg aaaaaacgat ggcagtgtga
     1081 atgggaaacg ctactttgaa tgccaggcca agtatggtgc ctttgtaaag ccatcagctg
     1141 tcaccgtagg agatttccca gaggaggact acgggttgga cgagatgtga ctgtacctgg
     1201 aaggaggccc cgcccctgac acagcccctc tgaatgcctg tggccctttt cctctgagat
     1261 ttgtgcatta aattaaacag aattcagaag gttg
//
