LOCUS       NM_001081396            3318 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus TBC1 domain family, member 31 (Tbc1d31), transcript
            variant 1, mRNA.
ACCESSION   NM_001081396 XM_977478
VERSION     NM_001081396.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154874.9, BC066830.1 and BC050039.1.
            
            On Nov 10, 2009 this sequence version replaced NM_001081396.1.
            
            Transcript Variant: This variant (1) represents the shorter
            transcript but encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC066830.1, SRR11927936.2689809.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-68                AC154874.9         135597-135664
            69-2461             BC066830.1         1-2393
            2462-2531           BC050039.1         2408-2477
            2532-3312           BC066830.1         2464-3244
            3313-3318           AC154874.9         193461-193466
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 24.08 cM"
     gene            1..3318
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /note="TBC1 domain family, member 31"
                     /db_xref="GeneID:210544"
                     /db_xref="MGI:MGI:2684931"
     exon            1..193
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..3107
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /note="isoform 1 is encoded by transcript variant 1; WD
                     repeat-containing protein 67; protein 4-B-3; WD repeat
                     domain 67"
                     /codon_start=1
                     /product="TBC1 domain family member 31 isoform 1"
                     /protein_id="NP_001074865.2"
                     /db_xref="CCDS:CCDS37077.2"
                     /db_xref="GeneID:210544"
                     /db_xref="MGI:MGI:2684931"
                     /translation="MQSTDLGNKESGKIWHRKPSPATHDGIIVNIVHSTSEYHPKVLR
                     FLNVAFDGSGDSFIAGDHQGNIYVFDLHGNRFNLVQRTAQACTALAFNLRRKSEFLVA
                     LADYSIKCFDTVTKELVSWMRGHESSVCSISVHASGRYAITTSSDTAQLWDLDTFQRK
                     RKLNIRQSVGIQKVFFLPLSNTILSCFKDNSIFAWECDTLFCKYQLPGPPEGSNILYK
                     VFAVTRDGRILAAGGKSNHLHLWCLEATGLFRIIQMPAKVRAVRHLEFLPDSFDAGSN
                     QVLGVLSQDGIMRFVNIQTCKLLFEIGTVEEGISSSVISPHGRYIASIMENGSLNVYS
                     VQALTQEINKPPPPLVKVIEDLPSNTVSSSNLKMKIMPGRVQRPARCKESKIPTRVLK
                     QDLTGDLENKENELSEGLNKKRLQILLKGYGEYPTKYRMFIWRSLLQLPENHTAFSSL
                     VDKGTHAAYLSLQKKYPIKSRKLLRVLQRTLSALAHWSAIFSDTPYLPLLAFPFVKLF
                     QNNQLICFEVVATLIINWCQHWFEYFPNPPINILSMIENVLAFHDKELLQHFIDRDIT
                     SQVYAWPLLETLFSEVLTREEWLRLFDNIFSNHPSFLLMTVVAYSTCSRAPLLNCTLK
                     NDFEYFFHHRNNLDINVVIREVYHLMETTPADIHPNSMLDAFVALTKGQYPIFNQYPK
                     FIVDYQTREWERIRNDELDFLRERQTVENMQAEVDEQRAKDEAWYQKQELLRRAEETR
                     REILLQEEEKMAQQRQRLAAVKRELEIKEIHLQDAARRRLLKLQQDQREMELRRLEDE
                     IERKVQMRDQEIAATAKDLEIRQLELEAQKRLYEKDLTTSQEAVAKEIREDTDAHRRK
                     AALEEHMFQKLLENSQMGGRRAQRWKEAEEKEFHLQSAKKASALSDASRKWFLRQETS
                     AALEHEEMPWLQRQYMDSAYLPQTSRLHDVSDMDPSTHIFSRNYPTEWNHMEHDLLKN
                     VRDLRRRLTARARNSCRHPHLLVT"
     exon            194..340
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            341..456
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            457..635
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            636..787
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            788..947
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1148
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1325
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1405
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1552
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1686
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1820
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..2000
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            2001..2233
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            2234..2386
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            2387..2522
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            2523..2615
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            2616..2756
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            2757..2877
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            2878..2973
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     exon            2974..3318
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3292..3297
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /note="hexamer: AATAAA"
     polyA_site      3312
                     /gene="Tbc1d31"
                     /gene_synonym="4B3; D330013L20Rik; Gm85; Wdr67"
                     /note="major polyA site"
ORIGIN      
        1 ggcggccgct cgaatttccc gggttgggcg cgcggggact gctgggtagc tctcgggacg
       61 gttaccctca ccgggtcagc cgcggatcgg agtcggggta ggggtcgggg ttcgacatgc
      121 agagcactga cctgggcaac aaggagagcg gcaagatatg gcaccgcaag ccatcgccag
      181 ccactcacga cggaattata gtgaacattg ttcacagcac ttctgaatac catcccaaag
      241 tcttgcggtt tttgaatgtg gcttttgatg gctcaggcga ttccttcatt gctggggacc
      301 atcagggaaa catctatgtt tttgacttgc atggaaacag gtttaatctt gttcagcgaa
      361 cagcacaagc gtgcacagct ctggccttta atcttcgtag aaagtctgag ttccttgtgg
      421 cgttagctga ttattctatt aaatgttttg atacagtcac caaggagctg gttagctgga
      481 tgagaggaca cgagtcgtcg gtgtgttcca tttctgtgca cgcatctggg agatacgcca
      541 tcaccacttc ctctgacaca gctcagctct gggacttgga tactttccag aggaagagaa
      601 agctgaacat ccgccagtcg gtgggcatac agaaggtttt ctttctacca ttaagtaaca
      661 ccatcctcag ctgttttaaa gacaactcca tcttcgcctg ggaatgtgat actctgtttt
      721 gcaaatacca attgccaggt ccacctgagg gctctaacat cttatacaaa gtgtttgctg
      781 tgaccagaga tggccgaatc ttggctgctg gaggcaaatc caatcacctg catttgtggt
      841 gcttggaggc cacagggctc ttcagaatca ttcagatgcc tgcgaaggta cgagctgttc
      901 gccatctgga attcctgcct gatagctttg atgccggttc caatcaggtt cttggtgtgc
      961 taagtcaaga tgggattatg cggtttgtca acatacagac ttgtaaactt ctctttgaaa
     1021 ttgggactgt tgaagaagga attagctcgt cagtaattag tccacacggt cggtacatag
     1081 catccattat ggaaaatgga agtctgaatg tatattcagt tcaggcttta acacaagaaa
     1141 taaataagcc tcctcctcct ctagttaagg tgattgaaga tttgcccagt aacacagtga
     1201 gttccagcaa tcttaaaatg aagataatgc caggaagagt acagcggcct gcaagatgta
     1261 aagagagcaa aattccaacc agggtgctca agcaggacct gactggggac ttagaaaaca
     1321 aggagaatga attgtcagaa ggattaaata aaaagcgctt acaaatctta ttaaaaggct
     1381 atggggaata tccaacaaaa tacagaatgt ttatctggcg ttccttacta cagctgccgg
     1441 aaaatcatac tgcatttagt agcttggtgg acaaagggac tcatgcggct tatctcagcc
     1501 ttcagaagaa ataccccatc aaaagcagga aactactgag agtattgcag agaaccctct
     1561 ctgcattagc tcactggtct gcaatcttca gtgacacacc ataccttcca ctcttggcat
     1621 ttccatttgt aaaattattc cagaacaatc agctcatctg ttttgaagtt gtggctactc
     1681 tcataatcaa ttggtgtcaa cattggtttg agtactttcc taatcctcct atcaacattc
     1741 ttagcatgat agaaaatgtt ttggcatttc atgacaagga gctgctgcag cacttcatag
     1801 atcgtgatat aacttcccag gtgtatgcct ggcctctcct ggaaacactg ttctcagaag
     1861 tgctgacgag agaggagtgg ctccgcctct tcgataacat cttctccaat cacccttcct
     1921 tcctcctgat gactgtggtg gcctacagta cctgctccag agcgcctttg ctcaactgta
     1981 ctcttaagaa tgactttgag tatttttttc accatcggaa taacctggac ataaatgttg
     2041 taattagaga agtttatcat ctcatggaga caacgcctgc tgatatccac ccaaatagca
     2101 tgctggatgc tttcgttgca ctgacgaaag ggcagtaccc tatatttaat caatatccaa
     2161 agttcattgt ggactaccag acacgggaat gggaaaggat aaggaacgat gaactggact
     2221 tcttgagaga gaggcagaca gttgaaaata tgcaagctga agtggatgag cagagagcta
     2281 aggatgaggc gtggtaccag aagcaggagc tgctccgcag agctgaggag acaagaagag
     2341 agatacttct tcaagaagag gagaagatgg cccagcagag acaaaggcta gctgccgtga
     2401 aaagggaact ggagataaag gaaatacact tacaggatgc tgcgaggaga cgcttgctga
     2461 agcttcagca agatcaacgg gaaatggagc tgcgcaggct ggaggatgaa attgagagaa
     2521 aggtacaaat gagagatcaa gaaatcgctg ccacagccaa ggacctggaa ataaggcagc
     2581 tggaactcga agcacaaaag cgactttatg agaaggatct tactacaagt caagaggctg
     2641 ttgccaaaga aatccgagaa gatacagatg cccataggcg gaaagcggct ctggaagaac
     2701 acatgttcca gaagctgcta gagaacagcc agatgggggg cagaagggcg cagaggtgga
     2761 aggaagccga agagaaggaa ttccatttgc aatcagcaaa gaaagcctct gccctttcag
     2821 atgcctctcg aaagtggttt ttaaggcagg agacaagtgc agctttggag catgaagaaa
     2881 tgccttggtt gcaaaggcag tacatggact cagcctatct gcctcaaacc tcacgattac
     2941 atgatgtttc tgacatggac ccctcaacac acattttttc aagaaattat ccaacagaat
     3001 ggaaccacat ggaacacgac cttctcaaga acgtgcgaga tcttcgcagg agactcacag
     3061 cccgggctcg gaacagctgt cggcaccctc atcttttggt gacataaaag acagtttacc
     3121 attaaacaat cagagatacc ttttttaatc aatggcattg attttttttc aactatttat
     3181 tctaattttt tataaagatc agccttttgt atagagaaat atgtctataa aattatgttt
     3241 tctattgaat tataatgctt tggcttgtaa agggcttctg gaatctttca caataaagat
     3301 ttttggaaac tatcaaag
//
