LOCUS       NM_172310               3194 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus threonyl-tRNA synthetase 3 (Tars3), mRNA.
ACCESSION   NM_172310
VERSION     NM_172310.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Zeng,Q.Y., Zhang,F., Zhang,J.H., Hei,Z., Li,Z.H., Huang,M.H.,
            Fang,P., Wang,E.D., Sun,X.J. and Zhou,X.L.
  TITLE     Loss of threonyl-tRNA synthetase-like protein Tarsl2 has little
            impact on protein synthesis but affects mouse development
  JOURNAL   J Biol Chem 299 (5), 104704 (2023)
   PUBMED   37059185
  REMARK    GeneRIF: Loss of threonyl-tRNA synthetase-like protein Tarsl2 has
            little impact on protein synthesis but affects mouse development.
REFERENCE   3  (bases 1 to 3194)
  AUTHORS   Zhou,X.L., Chen,Y., Zeng,Q.Y., Ruan,Z.R., Fang,P. and Wang,E.D.
  TITLE     Newly acquired N-terminal extension targets threonyl-tRNA
            synthetase-like protein into the multiple tRNA synthetase complex
  JOURNAL   Nucleic Acids Res 47 (16), 8662-8674 (2019)
   PUBMED   31287872
  REMARK    GeneRIF: Newly acquired N-terminal extension targets threonyl-tRNA
            synthetase-like protein into the multiple tRNA synthetase complex.
REFERENCE   4  (bases 1 to 3194)
  AUTHORS   Chen,Y., Ruan,Z.R., Wang,Y., Huang,Q., Xue,M.Q., Zhou,X.L. and
            Wang,E.D.
  TITLE     A threonyl-tRNA synthetase-like protein has tRNA aminoacylation and
            editing activities
  JOURNAL   Nucleic Acids Res 46 (7), 3643-3656 (2018)
   PUBMED   29579307
  REMARK    GeneRIF: Our results provided the first analysis of the
            aminoacylation and editing activities of ThrRS-L, and improved our
            understanding of Tarsl2.
REFERENCE   5  (bases 1 to 3194)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB642214.1, AK040927.1 and AV355827.1.
            
            On Apr 28, 2009 this sequence version replaced NM_172310.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC039225.1, AK079416.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                BB642214.1         2-28
            28-3191             AK040927.1         1-3164
            3192-3194           AV355827.1         250-252
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 35.23 cM"
     gene            1..3194
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /note="threonyl-tRNA synthetase 3"
                     /db_xref="GeneID:272396"
                     /db_xref="MGI:MGI:2444486"
     exon            1..317
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     CDS             48..2420
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /EC_number="6.1.1.3"
                     /note="probable threonyl-tRNA synthetase 2, cytoplasmic;
                     thrRS; threonyl-tRNA synthetase-like protein 2; probable
                     threonine--tRNA ligase 2, cytoplasmic; threonyl-tRNA
                     synthetase protein 3; threonyl-tRNA synthetase-like 2"
                     /codon_start=1
                     /product="threonine--tRNA ligase 2, cytoplasmic"
                     /protein_id="NP_758514.2"
                     /db_xref="CCDS:CCDS21339.1"
                     /db_xref="GeneID:272396"
                     /db_xref="MGI:MGI:2444486"
                     /translation="MAAQALAAQAVASRLQRQEEDIRWLCAEVQRLRDEQLRGPERGQ
                     AEGPRLTREVAQLQAENRDLHQRLCGLRLRLAEQRRTEAGRAAAHEPPTQNQEKDTKK
                     KRLKQSEPGREVKQPNFIKERLQLFETLKTDHQLLPATQEKKNTNNVISVRVAGGKTV
                     QGERWKTTPYQVAAGISKELAEHTVIAKVNGVLWDLDRPLEGDSTVELLMFDNEEAQA
                     VYWHSSAHILGEAMELYYGGHLCYGPPIENGFYYDMFIEDRVVSSTELSALENICKTI
                     IKEKQPFERLEVSKDTLLEMFKYNKFKCRILKEKVDTPTTTVYRCGPLIDLCKGPHVR
                     HTGKIKAIKIFKNSSTYWEGNPEMETLQRIYGISFPDSKMMKDWEKFQEEAKSRDHRK
                     IGKEQELFFFHDLSPGSCFFLPRGAFIYNALMDFIREEYHKRNFTEVLSPNMYNSKLW
                     ETSGHWQHYSNNMFTFDVEKDTFALKPMNCPGHCLMFAHRPRSWREMPVRFADFGVLH
                     RNELSGTLSGLTRVRRFQQDDAHIFCMVEQIEEEIKGCLHFLQSVYSTFGFSFQLNLS
                     TRPEHFLGEIEIWDEAERQLQNSLVEFGKPWKINPGDGAFYGPKIDIKIKDAIGRYHQ
                     CATIQLDFQLPIRFNLTYVSKDGDDKNRPVIIHRAILGSVERMIAILSENYGGKWPLW
                     LSPRQVMVIPVGPACENYALQVSKECFEEGFMADVDLDDSCTLNKKIRNAQLAQYNFI
                     LVVGEKEKINNAVNVRTRDNKIHGEISIASVIEKLKNLKKSRTLNAEEDF"
     exon            318..386
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            387..577
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            578..701
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            702..823
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            824..941
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1006
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1085
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1232
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1331
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1498
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1499..1661
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1799
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1877
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1878..1978
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1979..2083
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..2156
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2157..2271
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..3194
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3164..3169
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /note="hexamer: AATAAA"
     polyA_site      3192
                     /gene="Tars3"
                     /gene_synonym="A530046H20Rik; Tarsl2"
                     /note="major polyA site"
ORIGIN      
        1 gtcactctgg ctgggctggc gggctcaggt ggcacaggga agctaccatg gcggcccagg
       61 ccctggcggc tcaggcggtg gcctcgcgcc tgcagcggca ggaggaggac atccgctggc
      121 tgtgcgcgga ggtgcagcgc ctgagggacg agcagctgcg cgggcccgag cggggccaag
      181 ccgagggccc gcgcctgaca cgcgaggtgg cacagctcca ggcggagaac cgcgacctgc
      241 accagcgcct gtgtggcctg cggctgcgcc tggcggagca gcgccgcacc gaggccgggc
      301 gagcggcggc gcacgagcct cctacccaaa accaagagaa ggacacaaag aagaagaggc
      361 tgaagcagag tgagcctggc cgggaggtga agcaaccgaa ttttataaaa gagagattgc
      421 agctttttga aacattgaag acagatcatc aactgttacc tgctactcag gaaaagaaga
      481 acacaaacaa cgtgatttca gtgagggtgg ctggtgggaa gacagtgcaa ggagaaaggt
      541 ggaaaacaac accttatcaa gtggccgctg gaattagtaa ggaattggct gaacatacag
      601 tgattgccaa agtgaacggt gtattgtggg atctggaccg cccgttggaa ggggactcca
      661 ctgtggagct gctgatgttt gacaacgagg aagcccaggc tgtgtactgg cattccagcg
      721 ctcacatcct gggggaggcc atggaacttt actatggagg ccacctgtgt tatggcccac
      781 ccattgaaaa tgggttttat tatgatatgt ttattgaaga cagagtggtg tccagcactg
      841 agttgtctgc cctggagaac atatgtaaaa ctatcatcaa agaaaagcaa ccgtttgaaa
      901 gactggaagt cagcaaggac actctcctgg aaatgtttaa gtacaataaa tttaagtgcc
      961 ggattctgaa ggagaaagtg gacactccga cgaccactgt ttacaggtgt ggtccattga
     1021 ttgacctttg taaaggcccc catgtaagac acactggaaa aattaaagcc ataaaaattt
     1081 tcaagaactc ctcaacatat tgggaaggca accctgagat ggaaactttg cagcggatct
     1141 atgggatatc ctttcctgac agcaaaatga tgaaagactg ggaaaaattc caagaggaag
     1201 ccaagagccg ggaccacagg aaaatcggga aggaacaaga acttttcttc ttccatgatc
     1261 tgagccctgg aagctgtttt ttcctcccga gaggagcgtt catttacaat gcgcttatgg
     1321 atttcatacg agaggaatat cacaagcgta acttcacaga agtgctctct cccaacatgt
     1381 acaacagtaa actctgggaa acatcaggcc actggcagca ttacagtaac aacatgttca
     1441 cctttgacgt cgagaaggac actttcgccc tcaaacccat gaactgtcca gggcactgtt
     1501 taatgttcgc acatcgcccg cgatcctgga gggaaatgcc tgttagattt gctgattttg
     1561 gagttcttca cagaaatgag ctgtcaggca ctttaagcgg cttaacccga gtgaggcgct
     1621 tccagcaaga tgatgcgcac atcttctgca tggtggagca gatcgaagaa gaaataaagg
     1681 ggtgcttgca ctttttacag tccgtttact cgacgtttgg cttctccttt caattaaacc
     1741 tgtccacaag gcctgagcac ttcctaggag agattgagat atgggatgaa gctgaaaggc
     1801 aactgcagaa cagcttggtg gaatttggaa aaccatggaa aataaaccca ggagatggag
     1861 cattctatgg ccctaaaatc gatataaaaa ttaaagatgc tattggcaga taccatcagt
     1921 gtgctacaat tcagctggac ttccaactgc ctatcaggtt taacctcaca tatgtgagta
     1981 aggatgggga tgacaagaac aggcctgtga tcattcaccg cgccatcctg gggtctgtgg
     2041 aaaggatgat agccatcctc tcagagaact acggggggaa atggcctctc tggctgtccc
     2101 ctcgccaggt aatggtcatc ccagtaggcc cagcttgtga aaattatgca ctacaggtgt
     2161 ccaaggaatg cttcgaagaa ggcttcatgg ccgatgtgga cttagatgac agttgtacac
     2221 taaataagaa aatacggaat gcacagctgg ctcagtataa tttcattttg gtggttggcg
     2281 aaaaggaaaa gatcaacaat gcagtaaatg ttcgaacaag agacaacaaa attcatggag
     2341 aaatatcaat agcttccgtc attgaaaaat tgaagaacct caaaaaatca cgtacgctaa
     2401 atgccgagga agacttctga aggcttctcc ttgggcttct atataacctt gttttgactt
     2461 ctgaaatgta ttttccctgt cacttaagat attagtactt ctaagaaccc tagggccact
     2521 tgtagggctc taatgagttc aacctaagaa ggaaaagtga ttatttgata taaacaaaat
     2581 tttaatttgt tgtgttctga gactaaatag gagaagctgg agaaatttgc aagctatcaa
     2641 gactccagag acctcagtga taaaatggtg tccattcaaa gaggggaaca ctagcgagaa
     2701 ttatcagaaa tcatgctttg tttctttctc aaaagatgtg cttccagcct gggggctgta
     2761 gcttcgtgac cagagcatcc gcgttgcatg tatgaggcct gagctccatc cccagcagcc
     2821 accacctctc acccaacaat gacaggaata ggctttcaat aaaagcccat ggcccttttg
     2881 taacatggag gggagatgtc ttattttcta atacacacat ctcaggtatt tttatggctg
     2941 ctttactaaa gttcacactg aaactcatgc ccaaagctgg aattgctatt tcatttagtg
     3001 tgcaatcata agagaagtac taatatcctg ttagtggcaa aggactatga aacctcaaag
     3061 agattttgta caatctgtta tttgcattgt gagttaagag ttacagtttt aatgcttcta
     3121 atcataattc attatattta tctttagaaa cactaaagtc ctaaataaaa tgcatcacat
     3181 ggtgtttcta gagc
//
