LOCUS       NM_027931               2524 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus threonyl-tRNA synthetase 2, mitochondrial (putative)
            (Tars2), transcript variant 1, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_027931 XM_906121
VERSION     NM_027931.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Zheng,W.Q., Zhang,J.H., Li,Z.H., Liu,X., Zhang,Y., Huang,S., Li,J.,
            Zhou,B., Eriani,G., Wang,E.D. and Zhou,X.L.
  TITLE     Mammalian mitochondrial translation infidelity leads to oxidative
            stress-induced cell cycle arrest and cardiomyopathy
  JOURNAL   Proc Natl Acad Sci U S A 120 (37), e2309714120 (2023)
   PUBMED   37669377
REFERENCE   3  (bases 1 to 2524)
  AUTHORS   Tian,H., Yan,H., Zhang,Y., Fu,Q., Li,C., He,J., Li,H., Zhou,Y.,
            Huang,Y. and Li,R.
  TITLE     Knockdown of mitochondrial threonyl-tRNA synthetase 2 inhibits lung
            adenocarcinoma cell proliferation and induces apoptosis
  JOURNAL   Bioengineered 13 (3), 5190-5204 (2022)
   PUBMED   35184682
  REMARK    GeneRIF: Knockdown of mitochondrial threonyl-tRNA synthetase 2
            inhibits lung adenocarcinoma cell proliferation and induces
            apoptosis.
REFERENCE   4  (bases 1 to 2524)
  AUTHORS   Bushnell,E.A., Huang,W., Llano,J. and Gauld,J.W.
  TITLE     Molecular dynamics investigation into substrate binding and
            identity of the catalytic base in the mechanism of Threonyl-tRNA
            synthetase
  JOURNAL   J Phys Chem B 116 (17), 5205-5212 (2012)
   PUBMED   22482363
REFERENCE   5  (bases 1 to 2524)
  AUTHORS   Wolf,Y.I., Aravind,L., Grishin,N.V. and Koonin,E.V.
  TITLE     Evolution of aminoacyl-tRNA synthetases--analysis of unique domain
            architectures and phylogenetic trees reveals a complex history of
            horizontal gene transfer events
  JOURNAL   Genome Res 9 (8), 689-710 (1999)
   PUBMED   10447505
REFERENCE   6  (bases 1 to 2524)
  AUTHORS   Freist,W. and Gauss,D.H.
  TITLE     Threonyl-tRNA synthetase
  JOURNAL   Biol Chem Hoppe Seyler 376 (4), 213-224 (1995)
   PUBMED   7626230
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC093317.22, AK142685.1 and CO042173.1.
            
            On Jul 25, 2009 this sequence version replaced NM_027931.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK011540.1, BC147501.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-106               AC093317.22        116487-116592
            107-2442            AK142685.1         2-2337
            2443-2524           CO042173.1         1-82                c
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 40.93 cM"
     gene            1..2524
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /note="threonyl-tRNA synthetase 2, mitochondrial
                     (putative)"
                     /db_xref="GeneID:71807"
                     /db_xref="MGI:MGI:1919057"
     exon            1..220
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     CDS             140..2311
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /EC_number="6.1.1.3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     threonyl-tRNA synthetase-like 1; threonyl-tRNA synthetase,
                     mitochondrial; threonine--tRNA ligase, mitochondrial"
                     /codon_start=1
                     /product="threonine--tRNA ligase, mitochondrial isoform 1"
                     /protein_id="NP_082207.2"
                     /db_xref="CCDS:CCDS38548.1"
                     /db_xref="GeneID:71807"
                     /db_xref="MGI:MGI:1919057"
                     /translation="MGLCLRWRRLGFPLPEFRRCELHTVREASAPTPPHWLAERFGLF
                     EELWTAHVKKLASMTQKKARAIKISLPEGQKVDAVAWNTTPYQLAHQISVTLADTAVA
                     AEVNGELYDLDRPLETDCHLRFLTFDSPEGKAVFWHSSAHVLGAAAEQQLGAVLCRGP
                     STESGFYHDFFLGKERTVRSAELPILERICQELIAAAQPFRRLEASRDQLRQLFKDNH
                     FKLHLIEEKVTGPTATVYGCGMSVDLCRGPHLRHTGQIGALKLLTNSSALWRSLGAPE
                     TLQRVSGISFPKVELLRNWEARREAAELRDHRRIGKEQELFFFHELSPGSCFFLPRGT
                     RVYNALVAFIRAEYARRGFSEVKTPTLFSTKLWEQSGHWEHYRADMFSLKPPGTDGVD
                     NSQSGHPARCPKDTLALKPMNCPAHCLMFAHRPRSWRELPVRLADFGALHRAEASGSL
                     GGLTRLWRFQQDDAHIFCAPHQLEAEIQGCLDFLRCVYSVLGFSFHLALSTRPPGFLG
                     EPRLWDQAEQVLQQALEKFGEPWDLNPGDGAFYGPKIDVHLHDALGRPHQCGTIQLDF
                     QLPLRFDLQYKGPAGTPECPVLIHRAVLGSVERLLGVLAESCGGKWPLWLSPLQVVVI
                     PVRTEQEEYARQVQQCLQAAGLVSDLDADSGLTLSRRVRRAQLAHYNFQFVVGQREQS
                     QRTVNVRTRDNRQLGERDLAESVQRLLELQNARVPNAEEVF"
     exon            221..417
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            418..541
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            542..666
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            667..784
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            785..849
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            850..928
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            929..1075
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1174
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1392
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1555
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1693
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1694..1771
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1772..1872
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..1974
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1975..2047
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2162
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2163..2508
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2492..2497
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /note="hexamer: AATATA"
     polyA_site      2508
                     /gene="Tars2"
                     /gene_synonym="2610024N01Rik; Tarsl1; thrRS"
                     /note="major polyA site"
ORIGIN      
        1 gagttcctgg gggtggtgcc cggccggaat ggcggctatg ttatccaata ggaaaggata
       61 ttactgactt agaagccaat gaggagcctg gtgaggaaga agcggtgatc ggagcatgga
      121 ggtaccgatt tgagacagca tgggtctctg tctgaggtgg cgccggcttg ggttcccact
      181 cccagagttc cgccgctgcg agctccacac cgtgcgtgag gcctctgcac caactcctcc
      241 acattggttg gcagaacgat ttggcctttt tgaggagcta tggaccgctc acgtgaaaaa
      301 gttagcaagt atgacacaga agaaagcccg ggctattaag atatcacttc ctgaaggcca
      361 gaaggtagat gctgttgcat ggaacacaac cccttaccaa ctggcccatc agatcagtgt
      421 aacactggct gatactgcag tggctgctga agtaaatgga gaactttacg atctggaccg
      481 acccttggag acagattgtc acctcagatt tctgacattt gattccccag agggcaaagc
      541 ggtgttctgg cactctagtg cccatgttct gggggctgcg gctgagcaac aactgggtgc
      601 tgttctctgc cgaggtccaa gcacagaatc gggcttttac catgacttct tcctgggaaa
      661 agaacggaca gtccgcagcg cagagttgcc cattttagag cggatttgcc aggagctcat
      721 agctgctgca cagcctttcc ggaggctgga ggcttcacgg gatcagcttc gccagctctt
      781 caaggacaac cactttaagc ttcatctgat cgaggagaaa gtgacaggcc caacggcaac
      841 agtgtatggg tgtggcatgt cagttgacct gtgccgaggc ccccatcttc ggcacactgg
      901 acagattgga gcactgaagc tgctcacgaa ctcctcagcc ttgtggaggt ccttgggagc
      961 acctgagaca ctgcagaggg tatcaggaat ttctttcccc aaagtagagt tactgaggaa
     1021 ctgggaagct cgaagagaag cagcagagtt aagagaccac agacgcattg ggaaggaaca
     1081 ggagctcttc ttcttccatg aactgagccc tgggagctgt tttttcttgc cacgagggac
     1141 aagagtctat aatgccctgg tggctttcat cagggctgag tatgcccgcc gtggtttctc
     1201 agaggtgaaa actcccacgc tgttttctac aaaactctgg gaacagtcag ggcactggga
     1261 acactatagg gcagacatgt tttccctgaa gccccctggc actgatggtg ttgacaactc
     1321 ccagagtggc catcctgcca ggtgtcccaa agacacactt gctctaaagc ccatgaactg
     1381 ccctgcacac tgcctgatgt ttgcccaccg gcccagatcc tggagggaac tgcctgtgcg
     1441 actggctgat ttcggagccc tgcatcgggc tgaggcctct ggcagtctgg gaggattaac
     1501 gcggctgtgg cgcttccagc aggatgatgc gcacatcttt tgtgcacccc atcagctgga
     1561 agcagagatc cagggctgcc ttgattttct ccggtgtgtt tactcggttc ttggtttttc
     1621 cttccacctg gctttatcta cccggccacc tggtttccta ggggagcctc gcctatggga
     1681 ccaggctgag caggttctac agcaagcctt ggagaagttt ggagaacctt gggacctcaa
     1741 ccctggagat ggggctttct atgggcctaa gattgatgtg cacctccacg acgcccttgg
     1801 tcggccccat cagtgtggaa caatccagct tgacttccag ctgccactga gatttgacct
     1861 acaatacaag gggccggcag gtaccccaga gtgtccagtc cttattcatc gagcagtgct
     1921 tggttctgtg gaaaggctgt tgggagtgct ggcagaaagc tgtgggggga aatggcccct
     1981 gtggctgtcc ccgctccagg tggtggtcat ccctgtgagg acagagcaag aggaatatgc
     2041 caggcaggtg caacagtgcc tgcaggctgc aggacttgtg agtgatctcg atgcagactc
     2101 tggactgacc ctcagccgga gagtccgccg ggcccagctt gcccactaca actttcagtt
     2161 tgtggttggc cagagagagc agagtcaaag gacagtgaac gtacgcacgc gagataaccg
     2221 gcagctcggg gagcgagacc tggccgagtc cgtgcagaga ctgctggagt tacagaacgc
     2281 cagggtcccc aatgcagaag aagtgttctg agtcatgtcc attggtgatg tacagactca
     2341 tgagagccgt ccgtcccttt ccacgggaca gtaacccagc tggcagcccc gcatgtcccc
     2401 gaggcctcag aactgctctc tgaaaataga catggtgtgg gggctcccca cagaaaggaa
     2461 tgctgtactg actggataga aggagaataa aaatatactt gaagccaaaa aaaaaaaaaa
     2521 aaaa
//
