LOCUS       NM_031395               2671 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus synaptotagmin-like 3 (Sytl3), transcript variant 1,
            mRNA.
ACCESSION   NM_031395 XM_030250159
VERSION     NM_031395.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Kitamura,H., Nakagawa,T., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of FLJ
            genes: the complete nucleotide sequences of 110 mouse
            FLJ-homologous cDnas identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (2), 127-135 (2004)
   PUBMED   15449545
REFERENCE   3  (bases 1 to 2671)
  AUTHORS   Fukuda,M.
  TITLE     Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through
            interaction with the GDP-bound form of Rab27A in PC12 cells
  JOURNAL   J Biol Chem 278 (17), 15390-15396 (2003)
   PUBMED   12590134
REFERENCE   4  (bases 1 to 2671)
  AUTHORS   Fukuda,M.
  TITLE     The C2A domain of synaptotagmin-like protein 3 (Slp3) is an
            atypical calcium-dependent phospholipid-binding machine: comparison
            with the C2A domain of synaptotagmin I
  JOURNAL   Biochem J 366 (Pt 2), 681-687 (2002)
   PUBMED   12049610
REFERENCE   5  (bases 1 to 2671)
  AUTHORS   Kuroda,T.S., Fukuda,M., Ariga,H. and Mikoshiba,K.
  TITLE     The Slp homology domain of synaptotagmin-like proteins 1-4 and
            Slac2 functions as a novel Rab27A binding domain
  JOURNAL   J Biol Chem 277 (11), 9212-9218 (2002)
   PUBMED   11773082
  REMARK    GeneRIF: Synaptotagmin-like protein 3-a (Slp3-a) contains an
            N-terminal Slp homology domain (SHD) (PMID: 11327731). The SHD of
            Slp3-a specifically and directly binds the GTP-bound form of
            Rab27A.
REFERENCE   6  (bases 1 to 2671)
  AUTHORS   Fukuda,M., Saegusa,C. and Mikoshiba,K.
  TITLE     Novel splicing isoforms of synaptotagmin-like proteins 2 and 3:
            identification of the Slp homology domain
  JOURNAL   Biochem Biophys Res Commun 283 (2), 513-519 (2001)
   PUBMED   11327731
REFERENCE   7  (bases 1 to 2671)
  AUTHORS   Fukuda,M. and Mikoshiba,K.
  TITLE     Synaptotagmin-like protein 1-3: a novel family of C-terminal-type
            tandem C2 proteins
  JOURNAL   Biochem Biophys Res Commun 281 (5), 1226-1233 (2001)
   PUBMED   11243866
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC167249.3 and AC168090.3.
            
            On or before Apr 1, 2020 this sequence version replaced
            XM_030250159.1, NM_031395.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a, also known as
            Slp3-a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is based
            on transcript alignments.
            
            ##Evidence-Data-START##
            CDS exon combination :: AB057758.1, BC022608.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN00849375, SAMN00849381 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-208               AC167249.3         155075-155282
            209-743             AC167249.3         169477-170011
            744-962             AC167249.3         172228-172446
            963-1027            AC167249.3         183278-183342
            1028-1079           AC167249.3         195669-195720
            1080-1146           AC167249.3         197254-197320
            1147-1225           AC167249.3         198896-198974
            1226-1350           AC168090.3         118999-119123       c
            1351-1485           AC168090.3         115786-115920       c
            1486-1664           AC168090.3         109455-109633       c
            1665-1767           AC168090.3         96676-96778         c
            1768-1938           AC168090.3         91895-92065         c
            1939-2044           AC168090.3         90446-90551         c
            2045-2141           AC168090.3         89543-89639         c
            2142-2347           AC168090.3         88401-88606         c
            2348-2671           AC168090.3         86967-87290         c
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 4.33 cM"
     gene            1..2671
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /note="synaptotagmin-like 3"
                     /db_xref="GeneID:83672"
                     /db_xref="MGI:MGI:1933367"
     exon            1..208
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     exon            209..743
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     CDS             634..2457
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /note="isoform a is encoded by transcript variant 1;
                     synaptotagmin-like protein 3; exophilin-6"
                     /codon_start=1
                     /product="synaptotagmin-like protein 3 isoform a"
                     /protein_id="NP_113572.1"
                     /db_xref="CCDS:CCDS28372.2"
                     /db_xref="GeneID:83672"
                     /db_xref="MGI:MGI:1933367"
                     /translation="MAHEVDLESFKELERDIILRVLYRDQTVQSTEEERVRKLKSHLQ
                     HLRWKGAKSSSQEYKEKCCARCQRALGLLLNRGAVCQGCSHRVCSECRVFLRRTRAWK
                     CTVCFEDRNVKIKTGEWFFEERARKFPTAGRRETAGAKLLQSYQRLSKISVVPPTPPP
                     FSESQCSSSSRLQELGHFRGFNKSVENLFLSVTTQMRKLSKSQNDMTSEKHLLAMDPR
                     QCVGHTERRSQSDTAVNVTSRKASTPDILKAFHQEDPKHPPDPVLKQDTPPSSPTHSA
                     VFSGGLRHGSLISINSTCTEMGNFDNANVTGEIEFAIHYCVKSCSLEICIKTCKNLAY
                     GEEKKRKCNPYVKTYLLPDRSSQGKRKTRVQKNTLDPTFEETLKYQVDPGQLMTRRLQ
                     VSVWHLGTLARRVFLGEVILPLAMWDFKDSTAQNARWYPLRAKAEKYEENIPQNNGEL
                     AVRAKLVLPAGPRKPQEAQEGQLALNGQLCLVVLGAKNLPVRSDGTLNSFVKGCLTLP
                     NQQKLRVKSPVLKKQACPQWKHSFVFNGVSSSQLRQSTLELTVWDQAIFGMNDRLLGE
                     ARLGSKGGAAGCPDSGSQSKLQWHRVLSSPNLWTDMTLVLH"
     exon            744..962
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1027
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1079
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1146
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1225
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1350
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1485
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1664
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1767
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     exon            1768..1938
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     exon            1939..2044
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     exon            2045..2141
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     exon            2142..2347
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     exon            2348..2671
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2646..2651
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /note="hexamer: AATAAA"
     polyA_site      2671
                     /gene="Sytl3"
                     /gene_synonym="A430092N21; mFLJ00307; Slp3; Slp3-a;
                     Slp3-b"
                     /note="major polyA site"
ORIGIN      
        1 gcagtgtaaa atcctcatgt gagctccttg gcgtagcatt aggacgtctc cgctgtctgg
       61 gtccttggtg acgtgggatt agctacagcc acacttggga cagccatgta aaccatcagc
      121 ctcagccagt gcctggcagg caggaagaag acacacaggt caatggtgaa ggaactgact
      181 tcctcatcag gacacactcc agattaaggt tttaaggcat taatcaggct ggactctgtc
      241 acttctgctg acctggacat attttttctg gggaaatttt gctaccctaa gtcttagggc
      301 tctctcatga ctgctggtca acttcctcag gctggagatt actagcgctt cacagaaatt
      361 atctgctcag cagccatgca tcatttataa caatctctgg caaaacctca gaaaaagata
      421 cgcgcttgtc tcatctggca gagcccagtg aaaacactct tcgggtggaa gcgactccag
      481 ccgtggggca gcctcgtggg gtttgcttct ctgaaggagt atgacccagt taggaaggaa
      541 ggaagagcca cacgtcaaac acagtcacca gtgaaaccag agaggggctg gactccggct
      601 tctccacagg acagtggacc ccttctatcg gaaatggccc acgaagtgga cctggagtcc
      661 ttcaaggagt tggaacggga catcattctc cgtgtcctat atcgtgatca aactgttcaa
      721 agcaccgagg aggaaagggt ccggaaactg aaatcacacc tgcagcatct tcggtggaag
      781 ggggcgaaga gctctagcca ggaatacaag gaaaagtgct gtgcacgttg ccagcgggct
      841 ctgggccttc tgctgaaccg tggggccgtg tgtcagggct gcagccaccg cgtgtgttcc
      901 gagtgccgtg tgttcctgcg gaggactcgt gcatggaagt gcacggtttg ctttgaggac
      961 agaaacgtga aaatcaaaac cggagaatgg ttctttgaag aacgagccag gaaatttcca
     1021 actgcaggcc gacgcgagac cgctggggca aagctcctgc aatcttatca gaggctgagc
     1081 aaaatctctg tggtccctcc gacaccacca cctttcagtg agagccagtg cagcagctcc
     1141 agcaggttac aggaacttgg ccacttcaga ggatttaata agtctgtgga aaacttgttt
     1201 ctgtctgtca ccacccagat gagaaagctc tccaagtccc agaatgacat gacctctgag
     1261 aagcatcttc tggccatgga ccccaggcag tgtgtgggac acacggagag gcggagccag
     1321 tcagacactg cagtcaacgt gacttccagg aaggccagca cacccgatat tctgaaagca
     1381 ttccatcaag aagatcccaa acaccctccc gaccctgttt tgaagcaaga cactcccccg
     1441 tccagtccga cacacagcgc tgtcttctct ggaggcttga gacacggaag cttaattagc
     1501 atcaacagca cctgcacaga gatgggtaat tttgacaatg ctaatgtcac tggagaaata
     1561 gaatttgcca ttcattactg cgttaaaagc tgctctttag agatatgcat caagacctgt
     1621 aagaaccttg cctatggaga agaaaagaaa agaaagtgca atccgtatgt caagacctac
     1681 ctgctgcctg acaggtcttc tcagggaaaa cgcaagacac gagtccaaaa gaacacgctg
     1741 gacccgactt tcgaggaaac cttgaagtat caggtggacc ctggccagct gatgacccgg
     1801 aggctgcagg tctctgtgtg gcacctaggc acccttgccc ggagggtgtt cctcggagaa
     1861 gtgatccttc cactggccat gtgggacttt aaggacagta cggcacagaa tgccagatgg
     1921 tatcctctgc gggccaaggc tgagaaatac gaagagaata ttcctcagaa taatggagaa
     1981 cttgctgttc gagccaaatt ggtccttccc gcagggccaa gaaagcccca ggaggcccaa
     2041 gaaggtcagc tagcgcttaa tggtcaactg tgtctggttg tgttgggagc caagaattta
     2101 ccggtgaggt cagatggcac cttaaactca tttgttaagg gctgtctcac cctgccaaac
     2161 caacaaaagc tgcgtgtgaa gtccccagtc ctgaagaagc aggcttgtcc ccagtggaaa
     2221 cactcctttg tgtttaatgg tgtgagcagt tctcagctga gacagtccac cttggagctg
     2281 actgtctggg accaggccat ctttggtatg aacgaccgtc tgctcgggga agccaggctt
     2341 ggttcaaagg gaggtgctgc gggttgtccc gactcaggtt cacagtccaa gcttcagtgg
     2401 cacagagtcc tctccagccc aaacctgtgg acagacatga ccctcgtcct gcactgaggt
     2461 gaagcccaag gcgactgcga acccacaaag gtcaacagga agtgggatca tggcagaatc
     2521 agacacactg gatcaccaat aactgactac agtaatgtgt gtcgttagga cacggaacaa
     2581 ttactgttac ccaggcagtc ggggaggcta ctgtcaacag atagatgtgg aaatgttctg
     2641 atctcaataa acgctcaaca ctgtcctgta a
//
