LOCUS       NM_001285861            1382 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus synaptotagmin VIII (Syt8), transcript variant 4, mRNA.
ACCESSION   NM_001285861
VERSION     NM_001285861.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Monterrat,C., Boal,F., Grise,F., Hemar,A. and Lang,J.
  TITLE     Synaptotagmin 8 is expressed both as a calcium-insensitive soluble
            and membrane protein in neurons, neuroendocrine and endocrine cells
  JOURNAL   Biochim Biophys Acta 1763 (1), 73-81 (2006)
   PUBMED   16386321
REFERENCE   3  (bases 1 to 1382)
  AUTHORS   Hutt,D.M., Baltz,J.M. and Ngsee,J.K.
  TITLE     Synaptotagmin VI and VIII and syntaxin 2 are essential for the
            mouse sperm acrosome reaction
  JOURNAL   J Biol Chem 280 (21), 20197-20203 (2005)
   PUBMED   15774481
REFERENCE   4  (bases 1 to 1382)
  AUTHORS   Hutt,D.M., Cardullo,R.A., Baltz,J.M. and Ngsee,J.K.
  TITLE     Synaptotagmin VIII is localized to the mouse sperm head and may
            function in acrosomal exocytosis
  JOURNAL   Biol Reprod 66 (1), 50-56 (2002)
   PUBMED   11751263
  REMARK    GeneRIF: Syt VIII is a candidate for the Ca(2+) sensor that
            regulates acrosomal exocytosis in mammalian sperm
REFERENCE   5  (bases 1 to 1382)
  AUTHORS   Fukuda,M. and Mikoshiba,K.
  TITLE     Calcium-dependent and -independent hetero-oligomerization in the
            synaptotagmin family
  JOURNAL   J Biochem 128 (4), 637-645 (2000)
   PUBMED   11011146
REFERENCE   6  (bases 1 to 1382)
  AUTHORS   Mizutani,A., Fukuda,M., Ibata,K., Shiraishi,Y. and Mikoshiba,K.
  TITLE     SYNCRIP, a cytoplasmic counterpart of heterogeneous nuclear
            ribonucleoprotein R, interacts with ubiquitous synaptotagmin
            isoforms
  JOURNAL   J Biol Chem 275 (13), 9823-9831 (2000)
   PUBMED   10734137
REFERENCE   7  (bases 1 to 1382)
  AUTHORS   Fukuda,M., Kanno,E. and Mikoshiba,K.
  TITLE     Conserved N-terminal cysteine motif is essential for homo- and
            heterodimer formation of synaptotagmins III, V, VI, and X
  JOURNAL   J Biol Chem 274 (44), 31421-31427 (1999)
   PUBMED   10531343
REFERENCE   8  (bases 1 to 1382)
  AUTHORS   Ibata,K., Fukuda,M. and Mikoshiba,K.
  TITLE     Inositol 1,3,4,5-tetrakisphosphate binding activities of neuronal
            and non-neuronal synaptotagmins. Identification of conserved amino
            acid substitutions that abolish inositol 1,3,4,5-tetrakisphosphate
            binding to synaptotagmins III, V, and X
  JOURNAL   J Biol Chem 273 (20), 12267-12273 (1998)
   PUBMED   9575177
REFERENCE   9  (bases 1 to 1382)
  AUTHORS   Li,C., Ullrich,B., Zhang,J.Z., Anderson,R.G., Brose,N. and
            Sudhof,T.C.
  TITLE     Ca(2+)-dependent and -independent activities of neural and
            non-neural synaptotagmins
  JOURNAL   Nature 375 (6532), 594-599 (1995)
   PUBMED   7791877
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL603651.14 and AK075832.1.
            
            Transcript Variant: This variant (4) differs in the 5' UTR and
            coding sequence and uses an alternate in-frame splice junction at
            the 5' end of an exon compared to variant 1. The resulting isoform
            (c) has a longer and distinct N-terminus and lacks an alternate
            internal segment compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB026805.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-106               AL603651.14        98454-98559         c
            107-217             AK075832.1         211-321
            218-1382            AK075832.1         340-1504
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 87.93 cM"
     gene            1..1382
                     /gene="Syt8"
                     /note="synaptotagmin VIII"
                     /db_xref="GeneID:55925"
                     /db_xref="MGI:MGI:1859867"
     exon            1..106
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     CDS             100..1287
                     /gene="Syt8"
                     /note="isoform c is encoded by transcript variant 4;
                     synaptotagmin-8; sytVIII"
                     /codon_start=1
                     /product="synaptotagmin-8 isoform c"
                     /protein_id="NP_001272790.1"
                     /db_xref="CCDS:CCDS72070.1"
                     /db_xref="GeneID:55925"
                     /db_xref="MGI:MGI:1859867"
                     /translation="MQADRSMKMGHALNPFSTSAPLDATAGPSLIPDLITRIPWPRWT
                     LFIAILAAGVLLVSCLLCVICCYCHRHRHRKQPKDKETVGLGSARNSTTTHLVQPDVD
                     CLEPCSGGDQQWGRLLLSLEYDFGSQEIRVGLRQAGNLKAEGTADPYAWVSVSTQSGR
                     RHETKVHRGTLSPMFEETCCFLVPPAELPKATLKVQLWDFKRFSEHEPLGELQLPLGT
                     VDLQHVLESWYQLGPPGTTEPEQMGELCFSLRYVPSSGSLTVVVLEARGLNPGLAEAY
                     VKIQLMLNQRKWKKSKTSSKKGTTTPYFNEAFVFLVPVSQLQSVDLVLAVWARGLQLR
                     TEPVGKVLLGSRASGQPLQHWADMLAHARRPIAQWHHLRSPREVDRVLALQPRLPLLR
                     PRS"
     exon            107..217
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     exon            218..387
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     exon            388..486
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     exon            487..645
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     exon            646..813
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     exon            814..919
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1053
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1382
                     /gene="Syt8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1360..1365
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Syt8"
                     /note="hexamer: AATAAA"
     polyA_site      1381
                     /gene="Syt8"
                     /note="major polyA site"
ORIGIN      
        1 acatcatggt tccatgtgtg caggaggacc tctaaaggga gacggagaag gcagagactg
       61 gtcccttcct gatctgcagc tgccccgaat cactgagaga tgcaagctga taggagcatg
      121 aagatggggc acgccttaaa ccccttcagc acctctgccc cacttgatgc cacagctggg
      181 cccagtctca tcccagacct catcaccagg atcccctggc ctcgctggac cctctttatc
      241 gccatccttg ctgctggagt cctcttagtc tcctgtttgc tctgtgtcat ctgttgctat
      301 tgccaccgcc atcgccacag gaagcaaccc aaggataaag aaactgtggg cctgggcagt
      361 gcccgtaata gcaccactac tcacctggtt caaccagatg tggactgcct agaaccctgc
      421 tctggagggg accaacagtg gggtcgactc ctgctgtcac tagagtatga ttttggaagc
      481 caggagatta gagtgggcct gaggcaggct ggaaacctga aggctgaggg cacagcagac
      541 ccctacgcct gggtcagtgt gtccacccag tctgggagaa gacatgagac aaaggtgcac
      601 cgagggacgc tctctcccat gtttgaagag acatgctgct ttctggtccc accagcagag
      661 ctgcccaaag ccactctgaa ggtgcagctg tgggacttca agcggttttc agaacacgag
      721 ccactgggtg agctccaact acccctgggc accgtggacc ttcaacatgt cctggagagc
      781 tggtaccagc taggccctcc agggaccact gagcctgagc agatggggga gctgtgcttc
      841 tcactacgct acgtgcccag ctcaggcagc ctgactgtgg ttgtactgga ggctcggggt
      901 ctgaatccgg ggcttgcaga ggcctacgtg aagatccagc ttatgttaaa ccagagaaaa
      961 tggaagaaga gcaaaacatc ctccaagaaa ggcacgacta ccccctactt caacgaggcc
     1021 ttcgtcttcc tggttcccgt tagccagctc cagagtgtgg acctggtgct ggctgtctgg
     1081 gcccgtggtc tgcagctccg gactgagcct gtgggcaaag tgctgctggg ttcccgggct
     1141 tctggccaac ccctacagca ctgggcagac atgctggccc atgctcggcg gcccatcgcc
     1201 cagtggcacc acctgcggtc ccccagggaa gtggatcgtg ttctggccct gcagcctcgc
     1261 cttcccctgc ttaggcctcg ctcctgaaag caccgcgggt ctcagttttc ccagatggag
     1321 agctgtggat atttgtgcag gctcttcaga tctctctgta ataaacaaat tctctttcac
     1381 ac
//
