LOCUS       NM_001360422            1747 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus synaptotagmin V (Syt5), transcript variant 2, mRNA.
ACCESSION   NM_001360422 XM_006540189
VERSION     NM_001360422.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Weingarten,D.J., Shrestha,A., Orlin,D.J., Le Moing,C.L.,
            Borchardt,L.A. and Jackman,S.L.
  TITLE     Synaptotagmins 3 and 7 mediate the majority of asynchronous release
            from synapses in the cerebellum and hippocampus
  JOURNAL   Cell Rep 43 (8), 114595 (2024)
   PUBMED   39116209
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Rahman,M.M., Pathak,A., Schueler,K.L., Alsharif,H., Michl,A.,
            Alexander,J., Kim,J.A. and Bhatnagar,S.
  TITLE     Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to
            increase insulin secretion from pancreatic beta-cells improving
            glucose clearance
  JOURNAL   FASEB J 37 (8), e23075 (2023)
   PUBMED   37432648
REFERENCE   3  (bases 1 to 1747)
  AUTHORS   Seibert,M.J., Evans,C.S., Stanley,K.S., Wu,Z. and Chapman,E.R.
  TITLE     Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in
            Striatal Neurons by Regulating Substance P Secretion
  JOURNAL   J Neurosci 43 (9), 1475-1491 (2023)
   PUBMED   36732068
REFERENCE   4  (bases 1 to 1747)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1747)
  AUTHORS   Awasthi,A., Ramachandran,B., Ahmed,S., Benito,E., Shinoda,Y.,
            Nitzan,N., Heukamp,A., Rannio,S., Martens,H., Barth,J., Burk,K.,
            Wang,Y.T., Fischer,A. and Dean,C.
  TITLE     Synaptotagmin-3 drives AMPA receptor endocytosis, depression of
            synapse strength, and forgetting
  JOURNAL   Science 363 (6422) (2019)
   PUBMED   30545844
REFERENCE   6  (bases 1 to 1747)
  AUTHORS   Lang,J.
  TITLE     Molecular mechanisms and regulation of insulin exocytosis as a
            paradigm of endocrine secretion
  JOURNAL   Eur J Biochem 259 (1-2), 3-17 (1999)
   PUBMED   9914469
  REMARK    Review article
REFERENCE   7  (bases 1 to 1747)
  AUTHORS   Craxton,M., Olsen,A. and Goedert,M.
  TITLE     Human synaptotagmin V (SYT5): sequence, genomic structure, and
            chromosomal location
  JOURNAL   Genomics 42 (1), 165-169 (1997)
   PUBMED   9177789
REFERENCE   8  (bases 1 to 1747)
  AUTHORS   Fukuda,M., Kojima,T. and Mikoshiba,K.
  TITLE     Phospholipid composition dependence of Ca2+-dependent phospholipid
            binding to the C2A domain of synaptotagmin IV
  JOURNAL   J Biol Chem 271 (14), 8430-8434 (1996)
   PUBMED   8626542
REFERENCE   9  (bases 1 to 1747)
  AUTHORS   Morita,T., Kubota,H., Murata,K., Nozaki,M., Delarbre,C.,
            Willison,K., Satta,Y., Sakaizumi,M., Takahata,N., Gachelin,G. et
            al.
  TITLE     Evolution of the mouse t haplotype: recent and worldwide
            introgression to Mus musculus
  JOURNAL   Proc Natl Acad Sci U S A 89 (15), 6851-6855 (1992)
   PUBMED   1495973
REFERENCE   10 (bases 1 to 1747)
  AUTHORS   Willison,K.
  TITLE     The mouse Brachyury gene and mesoderm formation
  JOURNAL   Trends Genet 6 (4), 104-105 (1990)
   PUBMED   2132728
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161218.10.
            
            On Feb 3, 2018 this sequence version replaced XM_006540189.3.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11927932.2354525.1,
                                           SRR7345562.3002132.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-229               AC161218.10        162669-162897       c
            230-332             AC161218.10        161589-161691       c
            333-505             AC161218.10        160936-161108       c
            506-625             AC161218.10        158374-158493       c
            626-793             AC161218.10        158079-158246       c
            794-961             AC161218.10        157547-157714       c
            962-1079            AC161218.10        157183-157300       c
            1080-1213           AC161218.10        156436-156569       c
            1214-1747           AC161218.10        155121-155654       c
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 2.62 cM"
     gene            1..1747
                     /gene="Syt5"
                     /gene_synonym="SytV"
                     /note="synaptotagmin V"
                     /db_xref="GeneID:53420"
                     /db_xref="MGI:MGI:1926368"
     exon            1..229
                     /gene="Syt5"
                     /gene_synonym="SytV"
                     /inference="alignment:Splign:2.1.0"
     exon            230..332
                     /gene="Syt5"
                     /gene_synonym="SytV"
                     /inference="alignment:Splign:2.1.0"
     CDS             254..1414
                     /gene="Syt5"
                     /gene_synonym="SytV"
                     /note="synaptotagmin IX"
                     /codon_start=1
                     /product="synaptotagmin-5"
                     /protein_id="NP_001347351.1"
                     /db_xref="CCDS:CCDS20738.1"
                     /db_xref="GeneID:53420"
                     /db_xref="MGI:MGI:1926368"
                     /translation="MFPEPPTLGSPAPKTPPDSSRIRQGAVPAWVLATIVLGSGLLVF
                     SSCFCLYRKRCRRRMGKKSQAQAQVHLQEVKELGRSYIDKVQPEIEELDRSPSMPGQQ
                     VSDKHQLGRLQYSLDYDFQTGQLLVGILQAQGLAALDLGGSSDPYVSVYLLPDKRRRH
                     ETKVHRQTLNPHFGETFAFKVPYVELGGRVLVMAVYDFDRFSRNDAIGEVRVPMSSVN
                     LGRPVQAWRELQVAPKEEQEKLGDICFSLRYVPTAGKLTVIVLEAKNLKKMDVGGLSD
                     PYVKVHLLQGGKKVRKKKTTIKKNTLNPYYNEAFSFEVPCDQVQKVQVELTVLDYDKL
                     GKNEAIGRVAVGAAVGGAGLRHWADMLANPRRPIAQWHSLRPPDRARPIPAP"
     exon            333..505
                     /gene="Syt5"
                     /gene_synonym="SytV"
                     /inference="alignment:Splign:2.1.0"
     exon            506..625
                     /gene="Syt5"
                     /gene_synonym="SytV"
                     /inference="alignment:Splign:2.1.0"
     exon            626..793
                     /gene="Syt5"
                     /gene_synonym="SytV"
                     /inference="alignment:Splign:2.1.0"
     exon            794..961
                     /gene="Syt5"
                     /gene_synonym="SytV"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1079
                     /gene="Syt5"
                     /gene_synonym="SytV"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1213
                     /gene="Syt5"
                     /gene_synonym="SytV"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1747
                     /gene="Syt5"
                     /gene_synonym="SytV"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1725..1730
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Syt5"
                     /gene_synonym="SytV"
                     /note="hexamer: AATAAA"
     polyA_site      1747
                     /gene="Syt5"
                     /gene_synonym="SytV"
                     /note="major polyA site"
ORIGIN      
        1 ttctccgggg cagaacacgc acgcacacat atgcacaaat acacgcgcgc gcgcgcgcgc
       61 gatctcacac aggggcgcag acattagcct ggctaggctc agccgcgccc ccaccctgcc
      121 aaactgtgtg atcagcacac aagggaaagc gccatccacc cacgtgctgg gggcggggtc
      181 ccagcagccc acaacacctc cagaagggtc ctgtcgagac acctagcagg aagcaccgca
      241 ggaccccgac gccatgttcc cggaaccccc gaccctgggg tctccagcgc ctaagacacc
      301 tccagactcc agccgcatca ggcagggcgc agtgcctgcc tgggtcctgg ctaccatcgt
      361 gctgggctcg ggcctcctgg tcttcagcag ttgtttctgt ctctaccgga agcgctgtcg
      421 gagacggatg ggcaaaaaga gtcaggccca agcccaagtc catcttcagg aagtgaagga
      481 gctgggccgg agttacatag ataaggttca gcctgaaata gaggagctgg accggtcacc
      541 atccatgcct ggacagcagg tatcagacaa acaccagcta ggccgactgc agtattcact
      601 ggactatgac ttccagactg gccagctcct ggtgggcatc ttgcaagctc agggactggc
      661 tgcgttggac ctaggaggtt cctcagatcc ctatgttagt gtctatctgc tgccagacaa
      721 gcggaggcga catgagacca aggtgcatcg gcagaccctg aatccacact ttggagagac
      781 atttgccttc aaggtcccat acgtggaact agggggcaga gtgctggtca tggcggtgta
      841 tgactttgat cggttctccc gcaacgatgc catcggggag gtgcgggtgc ctatgagttc
      901 agtgaacctg gggcggccgg tgcaggcctg gagagagctg caggtggctc ccaaagagga
      961 gcaagagaaa ctgggggaca tctgcttctc tctccgttat gtccccacgg ccgggaagct
     1021 caccgtcatt gtcttggaag ctaaaaacct gaagaaaatg gatgtaggag gactctcaga
     1081 tccctatgtg aaggtgcacc tgctccaggg aggtaaaaag gttcgcaaga agaaaaccac
     1141 cattaagaag aacaccctga acccctatta caacgaggcc ttcagctttg aggtgccctg
     1201 tgaccaggtg cagaaagtcc aggtggagct gactgttctg gattatgaca aactggggaa
     1261 gaatgaggcc atcgggagag tggcggtggg cgcagcagtc ggtggggctg gcctgcggca
     1321 ctgggctgac atgttggcca accccaggcg ccccattgcc cagtggcact ctctgcggcc
     1381 ccctgacaga gccaggccaa taccagcacc ctgatttcca tcaagcaagc acctgacccc
     1441 agatcccttg ctcctagacc cttatccctc tccctgctcc ccaaacattg cattcattcc
     1501 tacttccaca ttggaaatgt ttccaagttc catccaatac cactcctata cccagaaacc
     1561 acagtgagcc tgcggggagg tgggagagcc gggaaagtct ggtatcccct gccccagggt
     1621 cctaccctct gctttgacaa tcagctgtga agcctctgat ttcctttttc ccagtctctt
     1681 acctcttccc acacagcttg actcctatgc tgctcttggg gccaaataaa atactcaata
     1741 gcttaga
//
