LOCUS       NM_009308               3901 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus synaptotagmin IV (Syt4), mRNA.
ACCESSION   NM_009308
VERSION     NM_009308.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3901)
  AUTHORS   Li,J., Sun,L., Peng,X.L., Yu,X.M., Qi,S.J., Lu,Z.J., Han,J.J. and
            Shen,Q.
  TITLE     Integrative genomic analysis of early neurogenesis reveals a
            temporal genetic program for differentiation and specification of
            preplate and Cajal-Retzius neurons
  JOURNAL   PLoS Genet 17 (3), e1009355 (2021)
   PUBMED   33760820
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3901)
  AUTHORS   Roome,R.B., Bourojeni,F.B., Mona,B., Rastegar-Pouyani,S., Blain,R.,
            Dumouchel,A., Salesse,C., Thompson,W.S., Brookbank,M., Gitton,Y.,
            Tessarollo,L., Goulding,M., Johnson,J.E., Kmita,M., Chedotal,A. and
            Kania,A.
  TITLE     Phox2a Defines a Developmental Origin of the Anterolateral System
            in Mice and Humans
  JOURNAL   Cell Rep 33 (8), 108425 (2020)
   PUBMED   33238113
REFERENCE   5  (bases 1 to 3901)
  AUTHORS   Spaiardi,P., Marcotti,W., Masetto,S. and Johnson,S.L.
  TITLE     Exocytosis in mouse vestibular Type II hair cells shows a
            high-order Ca2+ dependence that is independent of synaptotagmin-4
  JOURNAL   Physiol Rep 8 (14), e14509 (2020)
   PUBMED   32691536
  REMARK    GeneRIF: Exocytosis in mouse vestibular Type II hair cells shows a
            high-order Ca(2+) dependence that is independent of
            synaptotagmin-4.
REFERENCE   6  (bases 1 to 3901)
  AUTHORS   Vician,L., Lim,I.K., Ferguson,G., Tocco,G., Baudry,M. and
            Herschman,H.R.
  TITLE     Synaptotagmin IV is an immediate early gene induced by
            depolarization in PC12 cells and in brain
  JOURNAL   Proc Natl Acad Sci U S A 92 (6), 2164-2168 (1995)
   PUBMED   7892240
REFERENCE   7  (bases 1 to 3901)
  AUTHORS   Hilbush,B.S. and Morgan,J.I.
  TITLE     A third synaptotagmin gene, Syt3, in the mouse
  JOURNAL   Proc Natl Acad Sci U S A 91 (17), 8195-8199 (1994)
   PUBMED   8058779
REFERENCE   8  (bases 1 to 3901)
  AUTHORS   Morita,T., Kubota,H., Murata,K., Nozaki,M., Delarbre,C.,
            Willison,K., Satta,Y., Sakaizumi,M., Takahata,N., Gachelin,G. et
            al.
  TITLE     Evolution of the mouse t haplotype: recent and worldwide
            introgression to Mus musculus
  JOURNAL   Proc Natl Acad Sci U S A 89 (15), 6851-6855 (1992)
   PUBMED   1495973
REFERENCE   9  (bases 1 to 3901)
  AUTHORS   Perin,M.S., Fried,V.A., Mignery,G.A., Jahn,R. and Sudhof,T.C.
  TITLE     Phospholipid binding by a synaptic vesicle protein homologous to
            the regulatory region of protein kinase C
  JOURNAL   Nature 345 (6272), 260-263 (1990)
   PUBMED   2333096
REFERENCE   10 (bases 1 to 3901)
  AUTHORS   Willison,K.
  TITLE     The mouse Brachyury gene and mesoderm formation
  JOURNAL   Trends Genet 6 (4), 104-105 (1990)
   PUBMED   2132728
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CN666882.1, AK032106.1 and
            CAAA01195961.1.
            
            On Feb 23, 2005 this sequence version replaced NM_009308.2.
            
            Summary: The protein encoded by this gene belongs to the
            synaptotagmin family. Members of this family are multi-domained,
            integral membrane proteins of synaptic vesicles, and are thought to
            serve as Ca2+ sensors in the process of vesicular trafficking and
            exocytosis. This gene is primarily expressed in the nervous
            tissues. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC058208.1, SRR1660819.123904.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 CN666882.1         1-9
            10-2064             AK032106.1         2-2056
            2065-3901           CAAA01195961.1     6156-7992
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 17.75 cM"
     gene            1..3901
                     /gene="Syt4"
                     /gene_synonym="SytIV"
                     /note="synaptotagmin IV"
                     /db_xref="GeneID:20983"
                     /db_xref="MGI:MGI:101759"
     exon            1..282
                     /gene="Syt4"
                     /gene_synonym="SytIV"
                     /inference="alignment:Splign:2.1.0"
     CDS             249..1526
                     /gene="Syt4"
                     /gene_synonym="SytIV"
                     /codon_start=1
                     /product="synaptotagmin-4"
                     /protein_id="NP_033334.2"
                     /db_xref="CCDS:CCDS29109.1"
                     /db_xref="GeneID:20983"
                     /db_xref="MGI:MGI:101759"
                     /translation="MAPITTSRVEFDEIPTVVGIFSAFGLVFTVSLFAWICCQRRSAK
                     SNKTPPYKFVHVLKGVDIYPENLSSKKKFGGDDKSEVKGKAALPNLSLHLDLEKRDLN
                     GNFPKANPKAGSSSDLENVTPKLFTETEKEANSPESLKSSTSLTSEEKQEKLGTLFLS
                     LEYNFEKKAFVVNIKEAQGLPAMDEQSMTSDPYIKMTILPEKKHRVKTRVLRKTLDPV
                     FDETFTFYGIPYPHIQELSLHFTVLSFDRFSRDDVIGEVLIPLSGIELSDGKMLMTRE
                     IIKRNAKKSSGRGELLVSLCYQSTTNTLTVVVLKARHLPKSDVSGLSDPYVKVNLYHA
                     KKRISKKKTHVKKCTPNAVFNELFVFDIPCESLEEISVEFLVLDSERGSRNEVIGRLV
                     LGATAEGSGGGHWKEICDFPRRQIAKWHMLCDG"
     exon            283..1097
                     /gene="Syt4"
                     /gene_synonym="SytIV"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1218
                     /gene="Syt4"
                     /gene_synonym="SytIV"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..3901
                     /gene="Syt4"
                     /gene_synonym="SytIV"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3879..3884
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Syt4"
                     /gene_synonym="SytIV"
                     /note="hexamer: AATAAA"
     polyA_site      3901
                     /gene="Syt4"
                     /gene_synonym="SytIV"
                     /note="major polyA site"
ORIGIN      
        1 gtcaggattg cgtcaattcg ggtttcagcc acgtctggag tctcagagac aagcttttca
       61 gaagagccaa caggaacagg gcagggtggc acattgctga tagagagcgc ggaccttcct
      121 ccagcccctc ccgctgccgg tgctggtccc caatcccctg tgtttcccct ggaagccttg
      181 ttcctggatc cttccaggtg aatctgcccg gagttctttc agcacctcgg acagagcacg
      241 cagaaaacat ggctcctatc accaccagcc gcgtggaatt cgatgaaatt cccacagtgg
      301 tgggcatctt cagtgctttt ggcctcgtct tcactgtctc tctctttgcc tggatctgct
      361 gtcagagaag atcagccaaa tccaacaaga ctcctccata caagtttgtg cacgtgctta
      421 aaggagttga tatctaccca gaaaacctaa gtagcaaaaa gaagtttgga ggagatgaca
      481 agagtgaagt gaagggtaaa gccgctctgc ccaacctttc cctgcatctt gacctagaga
      541 agcgagacct caatggcaac ttccccaaag ccaaccccaa agctggcagc tcttctgatc
      601 tggaaaatgt caccccaaag ctctttacgg agacagaaaa ggaggccaat tcccctgaga
      661 gcttgaagtc cagcacttcc ctcacgtcag aggagaaaca agagaagctg gggacactct
      721 tcttgtctct agagtacaac ttcgagaaga aagcatttgt ggtgaacatc aaggaagccc
      781 agggcttacc agccatggac gagcaatcca tgacctctga cccgtacatc aaaatgacaa
      841 tcttaccaga gaagaagcac agagtgaaga ccagagtgct caggaagacg ctggaccctg
      901 tttttgatga aaccttcaca ttctatggaa tcccttatcc ccacatccaa gagctctcct
      961 tgcactttac agttctgagt tttgacaggt tttctagaga tgatgtcatt ggagaagtcc
     1021 tgattcctct ttcagggatt gaattatcag atggaaaaat gttaatgact agagagatca
     1081 tcaagagaaa tgctaagaag tcttctggcc ggggtgaact tctggtctct ctctgttatc
     1141 agtccactac aaacacgctc actgtggtgg tcttaaaagc gcggcaccta ccgaaatctg
     1201 atgtgtctgg actttcagat ccctacgtca aagtgaacct gtaccatgcc aagaagagaa
     1261 tctctaaaaa gaagactcac gttaagaaat gcactcccaa cgcagtgttc aatgaactgt
     1321 ttgtctttga tattccttgt gagagtcttg aagaaatcag tgttgaattc ttggttttgg
     1381 attctgaaag gggatcccga aatgaggtga ttggacggtt ggtcctgggt gccacagcag
     1441 aaggaagcgg tggggggcac tggaaggaga tctgtgactt ccccaggaga caaattgcta
     1501 agtggcacat gctctgtgat ggttagcttc ctggccatga gctgcaactt aaagattttt
     1561 actaggcaag aagcaatttt ctttcttata cacgattgta agcttgcagc acagcaagct
     1621 actttattat ttatttttat ttacttattt gttttggtta gaagtggatt aagttaatag
     1681 accagaaaat agttgtaaat gtgtattatg ataatttcct cttttattag aggtgttggc
     1741 caagttttca taagatattc agtttccctc tgtgtggtat taagtgggcc aacccagcct
     1801 gttatgagca ccctagctta accttgtcac agatgtgtgt tgtaggtgat ggtttcagtt
     1861 ttgcttggta gtggaaatca ggtgattgtt agagttaaaa atgagaacca cacctttttg
     1921 tatgaaaatt aatccatgtt tcttcaggtt gggggagata ggttttattt aaatccaaag
     1981 atatttccaa aatgtcctca agtctggttt tttttttttt agttaaatac gtcatgtaca
     2041 aatgattgtc ttatatatca aagtatgctg taattttcat ggtgattatt tgatacagtc
     2101 tcatcataag aagggtagct taggttcatt tcataaatac agcaaacaag ttgagaaatt
     2161 aatttataaa agggctgaat tgaagaacac tgtggttgat atgagatttt tttttgttcc
     2221 atcacatgtg agtcatgtaa aatttaactt cacctaagaa ccatggtctt gaattattca
     2281 ctgcctgtca gaagcctcag tgtggcctaa gatactccta tttaccattg tgaagttgtc
     2341 agactagtga aaaaaaataa acctaagcta gcaattcagt taggaataca tttttttaaa
     2401 tataaaaatc tatcagtgta caagtgtatt ttcaagattg tctttccccc tcagagtaga
     2461 catggctact taagttttta acttactctt accgagaaag aactaagcga cttaaaaaga
     2521 aagaaaagaa aagaaaaaaa aaacttgtgg aattcattgt caagttgaag tcaaccacat
     2581 taagtaaaag actaagacaa gaaaagagta agcctttcag aatttacaaa tattcacaaa
     2641 atacagttaa gctgtaaaaa tagcagagac agtgtgttct tcgctgtctt tagacgccag
     2701 tttgttttga gctgttattt ttaagatagc ttaaattatc gtgtcttgtg ccactcccaa
     2761 ttgctaagtt ctgaaattct tgcaaaacct gtgtgaggcg agcagagctc tggcatttcc
     2821 tgggaagact taattcaatg gcaatgtctt tatatttgag tcatcagaga ctcatgagta
     2881 tgacttaggt ccagccgaat tattaaatat gcaagttaga gataatgtct actggaaatt
     2941 ttacctttga gtcaagtttt gtgtcagtgc tttagcattt ttgagaatgt gtttgatatc
     3001 acagtgtttg taaattcgat gaaaatgtat ttctgaacca cttgtctgtg ctttttatga
     3061 ctacgacaaa actctgactt tctataagga gatatatttc attctgtttg tacaaaaatt
     3121 ttatgccttc ccctcactca tcgccatcca gtgacaacgt catctgacat ctctgcctac
     3181 tcagcagtct gagaataaca tggagtgagc tgcggtcatg tggagttggg gaggccaggc
     3241 ggcctctgca tgtatgtaca tttgatgtca taggccatgg aacctgtgga gcttggagct
     3301 ttaatgtcaa gagacttcat ttccaagtat cccacagaat ggcaggaagc tgatggtgac
     3361 ccaggttata ggatgtttgg tggaaaaaaa attttttttt ttaaaataga aaataaatct
     3421 gacaacaaat actttaaaaa ttgtaaatag tttttagttt tacgacttaa agtgttttga
     3481 tggggaaagt tgattgaaac tataactggt tttaagggaa aaaaaagaga gaaacacaag
     3541 aaaggagaaa agcaaggaaa aaaattgaaa caggcaggga gatatgcaat ctcattattt
     3601 ctgaaaaatt gtactgttta taatgtatta caatcttaac gtgaatatcc aattctgcta
     3661 aaattcctgc actgtattca acatgtacat tattattttt tgtgtgctta gccagtctaa
     3721 ccccccaaat cgggaggtta ccatgtccga ggaactgtgt gattcagtaa ctgacccgtt
     3781 ctgacttgtt gtagttcagt agtgatccgg agggaagcat ggtgttaggg agatgctgtc
     3841 tgttcctttg tgccagcact gtgtgtttgt aagacatgaa taaactgctg cttttgccca
     3901 a
//
