LOCUS       NM_001164483            7200 bp    mRNA    linear   ROD 01-OCT-2024
DEFINITION  Mus musculus synaptojanin 1 (Synj1), transcript variant 1, mRNA.
ACCESSION   NM_001164483 XM_001481287 XM_973263
VERSION     NM_001164483.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Kokotos,A.C., Antoniazzi,A.M., Unda,S.R., Ko,M.S., Park,D.,
            Eliezer,D., Kaplitt,M.G., De Camilli,P. and Ryan,T.A.
  TITLE     Phosphoglycerate kinase is a central leverage point in Parkinson's
            disease-driven neuronal metabolic deficits
  JOURNAL   Sci Adv 10 (34), eadn6016 (2024)
   PUBMED   39167658
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   Mohd Rafiq,N., Fujise,K., Rosenfeld,M.S., Xu,P. and De Camilli,P.
  TITLE     Parkinsonism Sac domain mutation in Synaptojanin-1 affects ciliary
            properties in iPSC-derived dopaminergic neurons
  JOURNAL   Proc Natl Acad Sci U S A 121 (17), e2318943121 (2024)
   PUBMED   38635628
  REMARK    GeneRIF: Parkinsonism Sac domain mutation in Synaptojanin-1 affects
            ciliary properties in iPSC-derived dopaminergic neurons.
REFERENCE   3  (bases 1 to 7200)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 7200)
  AUTHORS   Tian,Y., Yi,S., Guo,W., Feng,C., Zhang,X., Dong,H., Wang,K., Li,R.,
            Tian,Y., Gan,M., Wu,T., Xie,H. and Gao,X.
  TITLE     SYNJ1 rescues motor functions in hereditary and sporadic
            Parkinson's disease mice by upregulating TSP-1 expression
  JOURNAL   Behav Brain Res 452, 114569 (2023)
   PUBMED   37419331
  REMARK    GeneRIF: SYNJ1 rescues motor functions in hereditary and sporadic
            Parkinson's disease mice by upregulating TSP-1 expression.
REFERENCE   5  (bases 1 to 7200)
  AUTHORS   Zhu,X., Prakash,S.S., McAuliffe,G. and Pan,P.Y.
  TITLE     Synaptojanin1 Modifies Endolysosomal Parameters in Cultured Ventral
            Midbrain Neurons
  JOURNAL   eNeuro 10 (5) (2023)
   PUBMED   37072173
  REMARK    GeneRIF: Synaptojanin1 Modifies Endolysosomal Parameters in
            Cultured Ventral Midbrain Neurons.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7200)
  AUTHORS   Di Paolo,G., Sankaranarayanan,S., Wenk,M.R., Daniell,L.,
            Perucco,E., Caldarone,B.J., Flavell,R., Picciotto,M.R., Ryan,T.A.,
            Cremona,O. and De Camilli,P.
  TITLE     Decreased synaptic vesicle recycling efficiency and cognitive
            deficits in amphiphysin 1 knockout mice
  JOURNAL   Neuron 33 (5), 789-804 (2002)
   PUBMED   11879655
REFERENCE   7  (bases 1 to 7200)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   8  (bases 1 to 7200)
  AUTHORS   Cremona,O., Nimmakayalu,M., Haffner,C., Bray-Ward,P., Ward,D.C. and
            De Camilli,P.
  TITLE     Assignment of SYNJ1 to human chromosome 21q22.2 and Synj12 to the
            murine homologous region on chromosome 16C3-4 by in situ
            hybridization
  JOURNAL   Cytogenet Cell Genet 88 (1-2), 89-90 (2000)
   PUBMED   10773674
REFERENCE   9  (bases 1 to 7200)
  AUTHORS   Cremona,O., Di Paolo,G., Wenk,M.R., Luthi,A., Kim,W.T., Takei,K.,
            Daniell,L., Nemoto,Y., Shears,S.B., Flavell,R.A., McCormick,D.A.
            and De Camilli,P.
  TITLE     Essential role of phosphoinositide metabolism in synaptic vesicle
            recycling
  JOURNAL   Cell 99 (2), 179-188 (1999)
   PUBMED   10535736
REFERENCE   10 (bases 1 to 7200)
  AUTHORS   Khvotchev,M. and Sudhof,T.C.
  TITLE     Developmentally regulated alternative splicing in a novel
            synaptojanin
  JOURNAL   J Biol Chem 273 (4), 2306-2311 (1998)
   PUBMED   9442075
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC141885.3 and AC134560.5.
            
            On Apr 22, 2022 this sequence version replaced NM_001164483.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-331               AC141885.3         68713-69043
            332-477             AC141885.3         69967-70112
            478-564             AC141885.3         86109-86195
            565-832             AC141885.3         88489-88756
            833-1058            AC141885.3         91769-91994
            1059-1142           AC141885.3         92713-92796
            1143-1204           AC141885.3         98267-98328
            1205-1301           AC141885.3         99091-99187
            1302-1471           AC141885.3         100216-100385
            1472-1553           AC141885.3         101366-101447
            1554-1706           AC141885.3         103385-103537
            1707-1863           AC141885.3         106070-106226
            1864-1887           AC141885.3         108549-108572
            1888-2079           AC141885.3         110762-110953
            2080-2164           AC141885.3         112752-112836
            2165-2305           AC141885.3         115491-115631
            2306-2498           AC141885.3         115731-115923
            2499-2657           AC141885.3         116160-116318
            2658-2814           AC141885.3         118606-118762
            2815-2932           AC141885.3         119459-119576
            2933-3148           AC141885.3         119677-119892
            3149-3227           AC141885.3         122464-122542
            3228-3390           AC134560.5         1879-2041
            3391-3600           AC134560.5         5341-5550
            3601-3735           AC134560.5         6865-6999
            3736-3774           AC134560.5         7613-7651
            3775-3822           AC134560.5         9237-9284
            3823-3861           AC134560.5         10297-10335
            3862-3932           AC134560.5         10488-10558
            3933-4041           AC134560.5         12443-12551
            4042-4262           AC134560.5         12775-12995
            4263-7200           AC134560.5         18316-21253
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 52.18 cM"
     gene            1..7200
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /note="synaptojanin 1"
                     /db_xref="GeneID:104015"
                     /db_xref="MGI:MGI:1354961"
     exon            1..331
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     CDS             234..5057
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /EC_number="3.1.3.36"
                     /note="isoform a is encoded by transcript variant 1;
                     synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1"
                     /codon_start=1
                     /product="synaptojanin-1 isoform a"
                     /protein_id="NP_001157955.1"
                     /db_xref="CCDS:CCDS49907.1"
                     /db_xref="GeneID:104015"
                     /db_xref="MGI:MGI:1354961"
                     /translation="MRKSWTCWSGSDASGGCSGGGCGRRRRRSRRKRAASEERRMAFS
                     KGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGL
                     LGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVR
                     KVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDW
                     LLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQV
                     IYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYG
                     KQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSILK
                     PQVQKFLDYGFFYFDGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALG
                     LAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFF
                     DSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYK
                     YSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDI
                     FAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFI
                     RPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKER
                     NEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGD
                     QLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWP
                     FDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVE
                     AEERQKIYKEVIAVQGPPDGTVLVSIKSSAQESTFFDDALIDELLRQFAHFGEVILIR
                     FVEDKMWVTFLEGSSALNALSLNGKELLNRTITITLKSPDWIKHLEEEMSLEKISVTL
                     PSSASSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRT
                     SPCQSPTVPEYSAPSLPIRPSRAPSRTPGPPSSQGSPVDTQPAAQKDSSQTLEPKRPP
                     PPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGGVGAPPSPGVARREIEAPKSPGTA
                     RKDNIGRNQPSPQAGLAGPGPAGYGAARPTIPARAGVISAPQSQARVCAGRPTPDSQS
                     KPSETLKGPAVLPEPLKPQAAFPQQPSLPTPAQKLQDPLVPIAAPTMPPSGPQPNLET
                     PPQPPPRSRSSQSLPSDSSPQLQVKINGISGVKQEPTLKSDPFEDLSLSVLAVSKAQP
                     SVQISPVLTPDPKMLIQLPSASQSQVNPLSSVSCMPTRPPGPEESKSQESMGSSANPF
                     PSLPCRNPFTDRTAAPGNPFRVQSQESEATSWLSKEEPVPNSPFPPLMPLSHDTSKAS
                     SSLGGFEDNFDLQSQSTVKTSNPKGWVTFDEDDNFPTTGKSKSVCPDLVGNAPASFDD
                     DWSKGASVSFCVLPARRPPPPPPPVPLLPPGTTSSAGPSTTLPSKAPSTLDFTER"
     exon            332..477
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            478..564
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            565..832
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            833..1058
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1142
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1204
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1301
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1471
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1553
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1706
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1863
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            1864..1887
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..2079
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            2080..2164
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            2165..2305
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            2306..2498
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            2499..2657
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            2658..2814
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            2815..2932
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            2933..3148
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            3149..3227
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            3228..3390
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            3391..3600
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            3601..3735
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            3736..3774
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            3775..3822
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            3823..3861
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            3862..3932
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            3933..4041
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            4042..4262
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     exon            4263..7200
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7179..7184
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /note="hexamer: AATAAA"
     polyA_site      7200
                     /gene="Synj1"
                     /gene_synonym="A930006D20Rik; mKIAA0910"
                     /note="major polyA site"
ORIGIN      
        1 agagctacag ggctccacgc gaggaggacg gaccctccct ctgccggctc ccctggccgt
       61 gccaccttcc cactcccggg gcgacggagg ctcgcttcac ggacccagga cgcggcggcc
      121 gcaggaaccc ggtgaccagt aagcgccctg ctcggcagca cggtcccgcg tgggcggaaa
      181 cggctccgcc ctttcaccct aaggggcggg ccttccgcct gggcagcggc gcaatgcgga
      241 agagctggac ctgctggagc ggaagtgacg cttcgggcgg ctgtagcggc ggaggctgcg
      301 ggagaaggag gaggaggagc cggaggaaga gggctgcctc tgaagaaagg agaatggcgt
      361 tcagcaaagg atttcgaatc taccacaaat tggatccccc accgttcagc ctcatagtgg
      421 aaactaggca taaggaagaa tgtctcatgt tcgagtctgg ggctgtggct gtgctctcat
      481 ctgcagaaaa ggaggctatt aaaggcacat atgccaaagt actggatgca tacggacttt
      541 tgggagtttt acgattaaat cttggtgata ccatgctgca ctatctggtc ctagtcactg
      601 gatgcatgtc tgtggggaaa atccaagaat ctgaagtttt ccgagttact tccactgagt
      661 ttatatcatt gcgagttgat gcttcagatg aggaccgcat ttcggaagta cgaaaagttt
      721 tgaactcggg aaacttttat tttgcatggt ctgcatctgg agtcagctta gatctgagtc
      781 tgaatgcaca tcgaagcatg caagagcaca caaccgacaa taggtttttc tggaatcagt
      841 ctctgcattt gcatctcaag cactatggtg tgaactgcga tgactggtta ttacggctca
      901 tgtgtggggg agtagagatt agaaccattt atgctgccca taaacaggca aaagcttgcc
      961 tcatctcaag actaagctgt gaacgagctg ggacaaggtt caatgtccgg ggaaccaacg
     1021 atgatggtca tgttgccaac tttgtagaga cagagcaggt tatatactta gatgactgtg
     1081 tctcttcctt catacaaatc cgaggatctg ttccattgtt ctgggagcaa ccagggttgc
     1141 aagtgggatc ccatcgtgtt cgtatgtcaa ggggatttga agccaatgcg cctgcctttg
     1201 acaggcattt ccgaacactt aaggacttat atggtaaaca gatagtagtg aatctgcttg
     1261 ggtctaagga aggcgagcat atgctgagca aggctttcca gagtcattta aaagcctctg
     1321 aacacgcttc tgatatccac atggtgtctt ttgactatca tcaaatggtt aaaggaggaa
     1381 aggcagaaaa actacacagt attctcaaac cacaagtcca gaagtttcta gattatggat
     1441 ttttttattt cgatggcagt gaagttcaaa ggtgccagag tggtacagtt cgaacaaact
     1501 gcttggattg tctggataga acaaatagtg tgcaggcatt ccttggctta gagatgctcg
     1561 ctaagcagct ggaagctctg ggcttggctg agaagcctca gctggtgacc cgcttccaag
     1621 aagttttccg gtccatgtgg tctgtgaatg gcgactcaat cagtaaaata tatgcaggca
     1681 ccggggccct ggaagggaag gccaagttaa aagatggtgc gcgatctgtc accagaacca
     1741 tccagaataa cttcttcgac agctccaagc aggaagccat tgatgtcctg ctcctgggaa
     1801 atactctcaa cagcgattta gctgacaaag cccgagcgct tctaactact ggaagtttgc
     1861 gtgtttctga acagacatta cagtcagcat cttccaaagt cttaaagaac atgtgtgaga
     1921 acttctacaa atactcaaag cccaagaaga tccgagtgtg tgttggcacc tggaatgtga
     1981 acggcgggaa gcagttccgc agcatagcgt tcaagaacca gacgctcaca gactggcttc
     2041 tggacgctcc caagttagcc ggcatccagg agtttcaaga taagagaagt aagccaactg
     2101 atatatttgc aattggcttt gaagaaatgg tggagctgaa tgctgggaac attgtgaatg
     2161 caagcacaac caaccagaag ctgtgggcag tggagctgca gaagaccatc tccagggaca
     2221 acaagtatgt cctgctggcc tcggagcagc tggtgggtgt ctgcctgttt gtctttatca
     2281 gaccacagca cgctcctttt atcagggatg ttgcagttga tactgtgaaa actggcatgg
     2341 gaggtgcaac tggaaacaag ggcgccgttg caattcggat gctcttccac accaccagcc
     2401 tttgcttcgt ctgcagccac tttgctgcag ggcagtccca agtcaaagag agaaatgaag
     2461 attttgtaga aatagcacga aaactgagtt tccccatggg acgaatgctc ttctcccatg
     2521 actatgtatt ttggtgtggt gatttcaact atcgaattga ccttccgaat gaagaggtta
     2581 aagagctcat aagacaacaa aactgggatt ctctgatcgc tggggatcag ctcatcaacc
     2641 agaaaaatgc aggacagatc tttagaggat tcttagaggg aaaagtaacg tttgctccaa
     2701 cctataaata tgacttgttt tctgaagact atgacaccag tgagaagtgt cgcacccctg
     2761 cttggacaga ccgtgtcctc tggagaagga ggaagtggcc ttttgacaga tcagctgaag
     2821 atttagatct cctgaatgct agtttccaag atgaaagtaa aattctttat acatggaccc
     2881 ctggcacctt gctgcactat ggaagagccg agctgaagac ttctgaccat aggcccgtcg
     2941 ttgccttgat tgacatagat atatttgaag ttgaagctga agagagacaa aaaatttata
     3001 aagaagttat tgccgtccag ggcccaccag atggcacagt gctggtctca atcaaaagtt
     3061 ctgcacaaga aagtactttt tttgatgatg ctttgattga tgagcttctg cggcagtttg
     3121 cacactttgg tgaagttata ctcataagat ttgtagaaga taaaatgtgg gttacatttt
     3181 tggagggaag ctctgccttg aatgctctga gcctaaatgg gaaagagcta ttaaatcgga
     3241 ctataacaat tactttaaaa agtccagact ggatcaaaca tttggaagaa gagatgagtt
     3301 tagagaaaat cagtgtcacg ttgccctcat cagcaagctc caccctgctt ggtgaagacg
     3361 cagaggtcgc agcagacttt gacatggaag gtgacgtcga tgactacagc gctgaagtag
     3421 aggagcttct tcctcagcat ctgcagccgt cctcaagttc tggcctgggc acttctccaa
     3481 gttcttcacc ccggaccagt ccctgccagt cccccacagt accagagtac tccgcccctt
     3541 ctcttcccat cagacctagc cgagcaccgt caagaactcc aggacctcca agttcgcagg
     3601 gctctccagt tgacactcag ccagcggccc agaaggattc ttcccagact ctagagccca
     3661 agagaccacc ccctccccgc ccagtcgctc ctccggcacg tcctgcccca ccacagagac
     3721 cacctccacc ttcaggggct aggagtcctg cgcctgctag aaaagaattt ggaggtgttg
     3781 gagcccctcc cagtcccggg gttgctagga gagagataga agcacccaaa agccctggaa
     3841 cagcacggaa agataatata gggcgtaatc agccttcccc tcaagccgga cttgcaggcc
     3901 caggacccgc tggatacggt gcggctagac cgacaattcc agctcgtgct ggagtgatca
     3961 gcgcccctca gagccaggct cgggtatgtg ctggaaggcc gactcctgac agccaaagca
     4021 agccctcgga gacattgaaa ggtcctgctg tccttccaga accactgaag cctcaggctg
     4081 catttcctca gcagccttct ctgcccacac ctgctcaaaa gttacaggac ccgcttgtcc
     4141 ccatagcagc acctactatg cctccctctg gcccccaacc aaatttggaa acccccccac
     4201 agcccccacc tcggagcagg tcatctcaaa gcttgccttc agactcctca ccacagctgc
     4261 aggtaaaaat aaatggcatt tctggtgtca aacaagagcc aacattgaag agtgacccat
     4321 ttgaagatct ctcacttagt gtgcttgctg tatcaaaggc tcagccttct gttcagattt
     4381 cacctgttct aaccccagac ccaaagatgt tgattcagtt gccttctgca tcgcaaagtc
     4441 aagttaaccc tttgagttct gtaagttgca tgccaaccag gcctccaggt ccagaggaaa
     4501 gcaagtcaca ggagagtatg ggcagttctg caaacccgtt tcccagcctg ccctgccgga
     4561 acccttttac agacaggact gctgcccctg gaaacccatt tagagttcag tcccaagaat
     4621 cagaggcaac ttcttggctc tccaaagaag agcctgttcc caacagtccc ttccctcctc
     4681 tcatgcctct cagtcatgac acgagcaagg cttcaagttc gcttggtggc tttgaggaca
     4741 attttgatct gcagagccag tctacagtaa aaacaagcaa ccccaaagga tgggtaacct
     4801 ttgacgaaga cgacaacttt cctacaacag gaaagtcaaa gtcagtttgt ccagacttag
     4861 tgggtaacgc gccagcttca tttgatgatg actggagtaa aggtgcgagc gtttctttct
     4921 gtgtgttgcc agccagaagg ccaccaccac caccacctcc tgtccctctg ctaccacctg
     4981 gcacaacctc atctgcagga cctagcacaa ccctgccatc taaggcacct tccacattgg
     5041 acttcacgga aagataatta agtagggagg gcgggagttt ctcctcgtgt gtttggctgt
     5101 ttgtttctgt tttgtcaggg tggatccaag agaatggggc attagtcagt catgtgcaat
     5161 aaatgatcag taagagcact gatatttata taaaatacca ctggaggatg tggagaaagc
     5221 tggaatttgt gtatatcaga aataacaaag acatcctctt attaactgga gttctacttt
     5281 ttctcttcag atgaatagaa tagagcagga actattttta aaagtgctta aaactactct
     5341 agaaaatagt ccttattcgc atagtcacat aaaaatcacg taaagcccac acaactgcag
     5401 ctgacaggac tgcagcccat tgtgctgttg aggattggta gtcatggata cttaccaaag
     5461 agcttccaga gttctggtga gattgttgtg aaaggcatca ctttagtcaa aaggtctgtg
     5521 tatgtgtaca ctatgagtat atgtctgtat ttatgcacac aaacatgtat acatccacat
     5581 acttataaaa cttgatataa ttcagatcaa attttaccta acagttctga caatgtcctt
     5641 ttttcaatat ctgtcaagaa gatggccgcc accggctgtg ctttctatat tttgactgat
     5701 tgtgcatcct ggagtagaat aatcccttta gagaactgcc atttcctatt attctctacc
     5761 ccagcccttc agagtcaccc tcattacctt cctcctctgt acctctatga tgcctttctc
     5821 tgggcattta cagttggccg tctggacgtg gtcggtcacg tggacagata gaagagtggg
     5881 cgcacaccac cagcttcacg gaggaacatt gtccgtgtgc tcttttctct gcgtggccct
     5941 ttgcccttcc atacctttct gtttgccagt tgcctggagc tctgctttca gctgttgagt
     6001 ggtctgaatt aggaacatgt ccagtgtatg ttctcccatg taagaggcta catagcacat
     6061 ccatgatgct gctttaaggc tttagaggct atacctcaaa ttagctgtgg attttgtctc
     6121 ctgcactgcc agtatgcaac tgatcttacg tttattaatt ttgtgggaaa aaatacatgg
     6181 aatttttaca aaaaaacaaa aacaaaaaac aaacaaacaa aaaaaaaaca aaacaacaaa
     6241 aacatagtat tttgatatca ttagttaagc cagtcagaaa actccctaag ctattgtttg
     6301 gtcagttggt tgtcagtcag ctgaagttaa aacatcctta cccattaaga cttaatgttc
     6361 tctttatata aaaagagagt tatgtgctta cacccacgtt cctacatcta cctacctgaa
     6421 cctttaaaga tgtgtattgt aagattagaa aggacgtatg gctgccctca cctccgtgta
     6481 aaagcacagc aggctgcatc gcacttctct cctagagcag atctgttagg acagagcacc
     6541 tttgcaaatg ctcagtgtta tcacaatcct gtgtttatag aagtaactct gctgcagcca
     6601 tcactcgctg ttagcaataa ctcttgtgag ttttagatcg tgcttgttgg ggttctaatc
     6661 atttcaacag tagtagcttt taaactgcag tattactaga gcagtgtgct tcaaaatgtg
     6721 aattaacttg ttgagattgt ggctgtaaca tctgtgtgag tagtgtgtat ggtatccact
     6781 ccccattagc aagctaatcc actttagaaa actgaagcag tgtggatcgc agccctgagc
     6841 agtgactccc tcaggtgtga accactgtaa tgtctgcaga gcaatagctc tgccttgtca
     6901 ggaagtctgg tagctgctga aacgcacaaa gcaagttaaa gtttgctaca aagaactcga
     6961 catcgtgtgt atttgccagg tctggtcaaa gcagaaaaca gacactaaga atctctgtga
     7021 gtctctgttc tctctagtag attgctgttt atatgtatgt aagaggttta tcgcttatgt
     7081 ggcatacagt atttataact atatacttta tagaagtaca gtattaaagt tgctggtata
     7141 cattctgtac atatcttgtg agatgtgctg tcatatgaaa taaatatgtc tttatcacca
//
