LOCUS       NM_001281491            6014 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus synaptic Ras GTPase activating protein 1 homolog (rat)
            (Syngap1), transcript variant 1, mRNA.
ACCESSION   NM_001281491 XM_915205 XM_985548
VERSION     NM_001281491.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Vaissiere,T., Michaelson,S.D., Creson,T., Goins,J., Furth,D.,
            Balazsfi,D., Rojas,C., Golovin,R., Meletis,K., Miller,C.A.,
            O'Connor,D., Fontolan,L. and Rumbaugh,G.
  TITLE     Syngap1 promotes cognitive function through regulation of cortical
            sensorimotor dynamics
  JOURNAL   Nat Commun 16 (1), 812 (2025)
   PUBMED   39827187
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Jadhav,V., Carreno-Munoz,M.I., Chehrazi,P., Michaud,J.L.,
            Chattopadhyaya,B. and Di Cristo,G.
  TITLE     Developmental Syngap1 Haploinsufficiency in Medial Ganglionic
            Eminence-Derived Interneurons Impairs Auditory Cortex Activity,
            Social Behavior, and Extinction of Fear Memory
  JOURNAL   J Neurosci 44 (49), e0946242024 (2024)
   PUBMED   39406516
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6014)
  AUTHORS   Haetzel,L.M., Iafrati,J., Cording,K.R., Farhan,M., Noveir,S.D.,
            Rumbaugh,G. and Bateup,H.S.
  TITLE     Haploinsufficiency of Syngap1 in Striatal Indirect Pathway Neurons
            Alters Motor and Goal-Directed Behaviors in Mice
  JOURNAL   J Neurosci 44 (48), e1264232024 (2024)
   PUBMED   39358043
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6014)
  AUTHORS   Fenton,T.A., Haouchine,O.Y., Hallam,E.B., Smith,E.M., Jackson,K.C.,
            Rahbarian,D., Canales,C.P., Adhikari,A., Nord,A.S., Ben-Shalom,R.
            and Silverman,J.L.
  TITLE     Hyperexcitability and translational phenotypes in a preclinical
            mouse model of SYNGAP1-related intellectual disability
  JOURNAL   Transl Psychiatry 14 (1), 405 (2024)
   PUBMED   39358332
  REMARK    GeneRIF: Hyperexcitability and translational phenotypes in a
            preclinical mouse model of SYNGAP1-related intellectual disability.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6014)
  AUTHORS   McMahon,A.C., Barnett,M.W., O'Leary,T.S., Stoney,P.N.,
            Collins,M.O., Papadia,S., Choudhary,J.S., Komiyama,N.H.,
            Grant,S.G., Hardingham,G.E., Wyllie,D.J. and Kind,P.C.
  TITLE     SynGAP isoforms exert opposing effects on synaptic strength
  JOURNAL   Nat Commun 3, 900 (2012)
   PUBMED   22692543
  REMARK    GeneRIF: Overexpression of SynGAP alpha1 versus alpha2
            C-termini-containing proteins in hippocampal neurons has opposing
            effects on synaptic strength, decreasing and increasing miniature
            excitatory synaptic currents amplitude/frequency, respectively.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6014)
  AUTHORS   Porter,K., Komiyama,N.H., Vitalis,T., Kind,P.C. and Grant,S.G.
  TITLE     Differential expression of two NMDA receptor interacting proteins,
            PSD-95 and SynGAP during mouse development
  JOURNAL   Eur J Neurosci 21 (2), 351-362 (2005)
   PUBMED   15673435
  REMARK    GeneRIF: SynGAP expression peaked at times of synaptogenesis and
            developmental plasticity and SynGAP showed a more spatially
            restricted pattern as illustrated by its restriction to forebrain
REFERENCE   7  (bases 1 to 6014)
  AUTHORS   Vazquez,L.E., Chen,H.J., Sokolova,I., Knuesel,I. and Kennedy,M.B.
  TITLE     SynGAP regulates spine formation
  JOURNAL   J Neurosci 24 (40), 8862-8872 (2004)
   PUBMED   15470153
  REMARK    GeneRIF: The GAP activity of synGAP and its association with PSD-95
            are important for normal regulation of spine and synapse formation
            in hippocampal neurons.
REFERENCE   8  (bases 1 to 6014)
  AUTHORS   Tomoda,T., Kim,J.H., Zhan,C. and Hatten,M.E.
  TITLE     Role of Unc51.1 and its binding partners in CNS axon outgrowth
  JOURNAL   Genes Dev 18 (5), 541-558 (2004)
   PUBMED   15014045
REFERENCE   9  (bases 1 to 6014)
  AUTHORS   Kim,J.H., Lee,H.K., Takamiya,K. and Huganir,R.L.
  TITLE     The role of synaptic GTPase-activating protein in neuronal
            development and synaptic plasticity
  JOURNAL   J Neurosci 23 (4), 1119-1124 (2003)
   PUBMED   12598599
REFERENCE   10 (bases 1 to 6014)
  AUTHORS   Komiyama,N.H., Watabe,A.M., Carlisle,H.J., Porter,K.,
            Charlesworth,P., Monti,J., Strathdee,D.J., O'Carroll,C.M.,
            Martin,S.J., Morris,R.G., O'Dell,T.J. and Grant,S.G.
  TITLE     SynGAP regulates ERK/MAPK signaling, synaptic plasticity, and
            learning in the complex with postsynaptic density 95 and NMDA
            receptor
  JOURNAL   J Neurosci 22 (22), 9721-9732 (2002)
   PUBMED   12427827
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC144621.3.
            
            On Jun 25, 2019 this sequence version replaced NM_001281491.1.
            
            Summary: This gene encodes a Ras GTPase activating protein that is
            a member of the N-methyl-D-aspartate receptor complex. The
            N-terminal domain of the protein contains a Ras-GAP domain, a
            pleckstrin homology domain, and a C2 domain that may be involved in
            binding of calcium and phospholipids. The C-terminal domain
            consists of a ten histidine repeat region, serine and tyrosine
            phosphorylation sites, and a T/SXV motif required for postsynaptic
            scaffold protein interaction. The encoded protein negatively
            regulates Ras, Rap and
            alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor
            trafficking to the postsynaptic membrane to regulate synaptic
            plasticity and neuronal homeostasis. Homozygous null mutations
            result in early post-embryonic lethality, while heterozygous mutant
            mice display a variety of phenotypes that include learning and
            memory defects, hyperactivity, and audiogenic seizures. [provided
            by RefSeq, Nov 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            CCDS Note: This CCDS representation has an inferred exon
            combination due to a lack of full-length transcript support for
            this gene in mouse. This is likely to be one of several possible
            transcript variants for this gene. The exon structure of this
            representation is supported by human AB067525.2 and rat AF048976.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219382.178525.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-269               AC144621.3         171801-172069
            270-391             AC144621.3         175184-175305
            392-497             AC144621.3         177184-177289
            498-589             AC144621.3         183113-183204
            590-711             AC144621.3         183581-183702
            712-865             AC144621.3         185479-185632
            866-964             AC144621.3         185824-185922
            965-1588            AC144621.3         187534-188157
            1589-1733           AC144621.3         188284-188428
            1734-1878           AC144621.3         188628-188772
            1879-2115           AC144621.3         190160-190396
            2116-2317           AC144621.3         190483-190684
            2318-2496           AC144621.3         190894-191072
            2497-2538           AC144621.3         191620-191661
            2539-3601           AC144621.3         191987-193049
            3602-3775           AC144621.3         193465-193638
            3776-3987           AC144621.3         195378-195589
            3988-4078           AC144621.3         197434-197524
            4079-6014           AC144621.3         201050-202985
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 13.6 cM"
     gene            1..6014
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /note="synaptic Ras GTPase activating protein 1 homolog
                     (rat)"
                     /db_xref="GeneID:240057"
                     /db_xref="MGI:MGI:3039785"
     exon            1..269
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     CDS             203..4225
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /note="isoform 1 is encoded by transcript variant 1;
                     neuronal RasGAP; synaptic Ras-GAP 1; ras/Rap
                     GTPase-activating protein SynGAP; synaptic Ras
                     GTPase-activating protein 1; ras GTPase-activating protein
                     SynGAP"
                     /codon_start=1
                     /product="ras/Rap GTPase-activating protein SynGAP isoform
                     1"
                     /protein_id="NP_001268420.1"
                     /db_xref="CCDS:CCDS59625.1"
                     /db_xref="GeneID:240057"
                     /db_xref="MGI:MGI:3039785"
                     /translation="MSRSRASIHRGSIPAMSYAPFRDVRGPPMHRTQYVHSPYDRPGW
                     NPRFCIISGNQLLMLDEDEIHPLLIRDRRSESSRNKLLRRTVSVPVEGRPHGEHEYHL
                     GRSRRKSVPGGKQYSMEAAPAAPFRPSQGFLSRRLKSSIKRTKSQPKLDRTSSFRQIL
                     PRFRSADHDRARLMQSFKESHSHESLLSPSSAAEALELNLDEDSIIKPVHSSILGQEF
                     CFEVTTSSGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPP
                     KKRYYCELCLDDMLYARTTSKPRSASGDTVFWGEHFEFNNLPAVRALRLHLYRDSDKK
                     RKKDKAGYVGLVTVPVATLAGRHFTEQWYPVTLPTGSGGSGGMGSGGGGGSGGGSGGK
                     GKGGCPAVRLKARYQTMSILPMELYKEFAEYVTNHYRMLCAVLEPALNVKGKEEVASA
                     LVHILQSTGKAKDFLSDMAMSEVDRFMEREHLIFRENTLATKAIEEYMRLIGQKYLKD
                     AIGEFIRALYESEENCEVDPIKCTASSLAEHQANLRMCCELALCKVVNSHCVFPRELK
                     EVFASWRLRCAERGREDIADRLISASLFLRFLCPAIMSPSLFGLMQEYPDEQTSRTLT
                     LIAKVIQNLANFSKFTSKEDFLGFMNEFLELEWGSMQQFLYEISNLDTLTNSSSFEGY
                     IDLGRELSTLHALLWEVLPQLSKEALLKLGPLPRLLNDISTALRNPNIQRQPSRQSER
                     TRSQPMVLRGPSAEMQGYMMRDLNSSIDLQSFMARGLNSSMDMARLPSPTKEKPPPPP
                     PGGGKDLFYVSRPPLARSSPAYCTSSSDITEPEQKMLSVNKSVSMLDLQGDGPGGRLN
                     SSSVSNLAAVGDLLHSSQASLTAALGLRPAPAGRLSQGSGSSITAAGMRLSQMGVTTD
                     GVPAQQLRIPLSFQNPLFHMAADGPGPPAGHGGSSGHGPPSSHHHHHHHHHHRGGEPP
                     GDTFAPFHGYSKSEDLSSGVPKPPAASILHSHSYSDEFGPSGTDFTRRQLSLQDSLQH
                     MLSPPQITIGPQRPAPSGPGGGSGGGSGGGQPPPLQRGKSQQLTVSAAQKPRPSSGNL
                     LQSPEPSYGPARPRQQSLSKEGSIGGSGGSGGGGGGGLKPSITKQHSQTPSTLNPTMP
                     ASERTVAWVSNMPHLSADIESAHIEREEYKLKEYSKSMDESRLDRVKEYEEEIHSLKE
                     RLHMSNRKLEEYERRLLSQEEQTSKILMQYQARLEQSEKRLRQQQVEKDSQIKSIIGR
                     LMLVEEELRRDHPAMAEPLPEPKKRLLDAQERQLPPLGPTNPRVTLAPPWNGLAPPAP
                     PPPPRLQITENGEFRNTADH"
     exon            270..391
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            392..497
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            498..589
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            590..711
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            712..865
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            866..964
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1588
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1733
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..1878
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..2115
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            2116..2317
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            2318..2496
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            2497..2538
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            2539..3601
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            3602..3775
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            3776..3987
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            3988..4078
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     exon            4079..6014
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5992..5997
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /note="hexamer: AATAAA"
     polyA_site      6014
                     /gene="Syngap1"
                     /gene_synonym="Gm1963; Syngap"
                     /note="major polyA site"
ORIGIN      
        1 ctctctctgc tgccgctgct gccgttggct cttattctcc tcctcctcct cctctctcct
       61 cctctctgct cctctccgct cctctctcct cctctctcct cctcctcctc ctcctccacc
      121 tcctccttct ccccctttct ctccccctct ttctctcttc tttttctccc cccgtccccc
      181 cgccccctcc ccctcaggcc tgatgagcag gtctcgagcc tccatccatc gggggagcat
      241 ccccgcgatg tcctatgccc ccttcagaga tgtacgggga ccccctatgc accgaaccca
      301 atacgttcat tccccgtacg atcgtcctgg ctggaaccct cggttctgca tcatctcggg
      361 gaaccagctg ctcatgctgg atgaggatga gatacacccc cttctgatcc gcgaccggag
      421 gagcgagtcc agccgaaaca aactgctgag acgcaccgtc tctgtgccag tggaggggcg
      481 gccccacggc gagcatgaat accacttggg tcgctcgagg aggaagagtg tcccaggggg
      541 gaaacagtac agcatggagg ccgcccccgc tgcgcccttc cggccctcgc aaggcttcct
      601 gagccggagg ctaaaaagct ctatcaaacg tacaaagtca caacccaaac ttgaccggac
      661 cagcagcttt cgacagatcc tgcctcgctt ccgaagtgct gaccatgacc gggcccggct
      721 gatgcagagc ttcaaggagt cacattccca cgagtccctg ctgagtccca gtagtgctgc
      781 tgaggccttg gagctcaacc tggatgaaga ctccattatc aagccagtac acagctccat
      841 cctgggtcag gagttctgct ttgaggtaac aacatcatct ggaacaaaat gctttgcctg
      901 tcggtctgca gctgaaaggg acaaatggat tgagaatctg cagagggctg taaaacccaa
      961 caaggacaac agccgccgag tagataacgt gctgaagcta tggatcatag aggctcgaga
     1021 gctgcccccc aagaagagat attactgtga gctgtgcctg gacgacatgc tgtatgcacg
     1081 aaccacctcc aagccccgct cggcttcagg agacaccgtc ttttggggcg agcactttga
     1141 gtttaacaac ctgcctgccg tccgggccct tcggctgcat ctgtaccgtg actcagacaa
     1201 aaagcggaag aaggacaagg ctggctacgt tggcctggtg actgttccag tggccaccct
     1261 agctgggcgc cacttcacag agcagtggta ccccgtgacc ttgccgacag gcagtggggg
     1321 ctctgggggc atgggctcgg ggggaggagg agggtcaggg ggtggctcag ggggcaaagg
     1381 gaaaggaggt tgtcctgctg tgagactgaa agcccgttac cagacgatga gcatcctgcc
     1441 catggagctg tataaggagt ttgcagagta tgtgaccaac cattaccgga tgctatgtgc
     1501 agtgctggag cctgccctga atgtcaaagg caaggaagag gtggccagtg cactggttca
     1561 catcctgcag agcacaggca aggccaagga cttcctttca gacatggcca tgtcagaggt
     1621 agaccgattc atggagcggg aacacctcat attccgagag aacacgctcg ccactaaagc
     1681 catagaagag tatatgagac tgattggcca gaaatacctc aaggatgcca ttggggagtt
     1741 cattcgtgct ctgtatgaat ctgaggagaa ctgtgaagta gaccccatca agtgcacagc
     1801 gtccagtctg gcagagcacc aggccaacct gcggatgtgc tgtgagttgg ccctgtgcaa
     1861 ggtggtcaac tcccattgcg tgttcccgag ggagctgaag gaggtgtttg catcttggcg
     1921 gctgcgctgc gcagagcggg gccgagagga cattgctgac aggctgatca gcgcctcgct
     1981 cttcctgcgc ttcctctgcc cggccattat gtcgcccagt ctatttgggc ttatgcagga
     2041 gtacccagat gagcagacct cacgaaccct caccctcatc gccaaggtca tccagaacct
     2101 ggccaacttt tccaagttta cctcaaagga ggacttcctg ggcttcatga atgagtttct
     2161 ggagctggag tggggctcta tgcagcagtt cttgtatgag atatccaacc tggacacctt
     2221 gaccaacagc agcagttttg agggctatat agacttgggc cgcgagctct ccacacttca
     2281 cgccctgctc tgggaggtgc tgccccagct cagcaaggaa gccctcctga agctgggccc
     2341 actgccccgg ctcctcaatg acatcagcac agccctgagg aaccctaaca tccaaaggca
     2401 gccaagccgc cagagtgaac ggactcggtc tcagcccatg gtgctgcgag ggccatcagc
     2461 cgagatgcag ggctacatga tgcgggacct caatagctcc atcgaccttc aatccttcat
     2521 ggctcgaggc ctcaacagct ctatggacat ggctcgcctc ccctccccaa ccaaggaaaa
     2581 accaccacca ccaccacccg gtgggggtaa agaccttttc tatgtgagcc ggccaccact
     2641 ggcccggtcc tccccagcat actgcacgag cagctcggac atcacagagc cagagcagaa
     2701 gatgctgagt gtcaacaaga gtgtgtccat gctggacctt cagggcgacg ggcctggagg
     2761 tcgccttaac agtagcagtg tttccaacct ggcagctgtt ggggacttgt tgcattccag
     2821 ccaggcctcg ctgacagcag ctttggggtt gcggcctgca cctgccgggc gcctctccca
     2881 ggggagtggc tcttccatca cagcagctgg catgcgcctc agccagatgg gggtcactac
     2941 agatggtgtc cccgcccagc aactgcgcat ccccctttcc ttccagaacc ctctcttcca
     3001 tatggctgct gatgggccag ggcccccagc aggccatgga gggagcagtg gtcatggtcc
     3061 accttcctcc catcaccacc accaccacca tcaccaccac cgagggggag aacccccagg
     3121 ggacactttt gccccattcc atggctatag caagagcgag gacctctctt caggggtccc
     3181 taagcccccc gccgcctcca tccttcacag ccacagctac agcgacgagt ttggaccctc
     3241 tggcactgat tttacccgcc ggcagctctc gcttcaggac agtctacagc acatgctctc
     3301 ccctccccag attaccatcg gtccccagag gccagctccc tcagggccgg gagggggcag
     3361 cggcgggggc agcggtgggg gccagccacc ccccttgcag aggggcaagt ctcaacagtt
     3421 gacagtgagt gccgcccaga aacctcggcc gtctagcggg aacctgttgc agtccccgga
     3481 gccaagctac ggacctgccc gtcctcggca gcagagcctc agcaaagagg gcagcattgg
     3541 gggcagcggg ggcagcgggg gcggaggggg tggggggctc aagccctcca tcaccaagca
     3601 gcattcccag actccatcca cgcttaaccc cacaatgcca gcctcggagc ggaccgtagc
     3661 ctgggtctcc aacatgcctc acctgtcggc tgacatcgag agtgcacaca tcgagcgaga
     3721 agagtacaag ctcaaggagt actccaagtc catggacgaa agccggctgg acagggtgaa
     3781 ggagtatgag gaggagatac attcgctgaa ggagaggcta cacatgtcca accggaagct
     3841 ggaagagtat gagcggaggt tgctgtccca ggaagaacag accagcaaga tcctgatgca
     3901 gtaccaagcc cgcctggagc agagtgagaa gcgcttgaga cagcagcagg tggagaagga
     3961 ctcccagatc aagagcatca ttggcaggct gatgctggtg gaggaggagc tgcgtcggga
     4021 ccaccccgcc atggctgagc cgctgcctga gcccaagaag aggctgctcg acgctcagga
     4081 gaggcagctt ccccccttgg gtccaacaaa cccgcgtgtg acgctggccc caccttggaa
     4141 cggcctggcc cccccagccc cacccccccc accccggctg cagatcacag agaacggcga
     4201 gttccggaac accgcagacc actagcccac ccagcatcac agacctcctt ccctgtgcac
     4261 cctaccccag cccacccagc gtcacagacc tccttccttg tgcaccccac cctggaacat
     4321 caccaaccac caggactgga cgtcgccaag ggacagcggg attgtctccc ttaatgcctc
     4381 cttggggcac ccatctgccg accccactac tccattctag gagggagagt gggaccctca
     4441 gctgccctct ttcaccccag tacaccacct accccacaca gaccccttca ctctggggtg
     4501 ctatccctgt cctctgcctc atagttcccc tgagcactgg ggggacagac cctcaccccc
     4561 acactggggg tgtggcacct ccaaactttc aacttcaggg tgatttttta gcagtaacca
     4621 gagctgacaa tctataactc ccctctacca ccccattttg gcctcccctg cccccccttg
     4681 ttatggggag gggaccccgg gtgagggggc cctattaccc cttgatttct caggagcgtc
     4741 tgggggggct cagcacgcac aaactccttc tcctctttcc actcttacat taactctccc
     4801 cacccagaac ccagatgggg tggagggggc caccggggca gggagggggc ggcaaggggg
     4861 gaatgggagt tgcctcccct tcttcccaca cctgatctgc tcttggctgg tcccagagcg
     4921 gggtgagggg gcttatgccc ccccctcccc cagtgtgttg ggtggggtgg aattgaggtt
     4981 tggatgaggg ggttagggta taggaggcca tgtgtgttgg gttgtggagg gacagactag
     5041 ctgatctgcc ctaccctgac atgcagccag ccctctaccc cttccctctc ttgtctctac
     5101 tcccatgggg aggggggaac ttactctagg aaaagccatg tctctctccc ccaggatggg
     5161 gggacctgtg ttggaggatg ggtgtttggg ggccccctta catgactctg tcccctgggg
     5221 gaggtaggac agggctgggc ttccctctca ccctcaccct cacccagtct ccctccctcc
     5281 cgccagctcc atgctttttc ggtgtttttc tgtacatagc tctctggcgg tataggggag
     5341 gtaggatgga tggggtttgg ggtgggtagg ccatggaagg ggagaaaccc ctcccgggta
     5401 ccccctcttc tctgactgct gccccctacc cagccttgtc tcttcactcc ttgcgtttgg
     5461 tattgagaat tcttcccaga ctccagactc gatctttctt ttgtatggac agctctcctt
     5521 cggtctaacc ctctacatac acccacccag ccggggccaa atttatactt atataaaagt
     5581 tgtaaatatg tgaaatttta tcccatgccc tttcctctgc tttccccatc aggccttacc
     5641 cttgaacctc ctccctcctc tttggctgtt gtaattatct ggggtttgta ctgtacataa
     5701 ttggggaggg gggctgggag gcgacccctc tgtacagagc ttcctggctc cctagcccca
     5761 cccctcactt ccctccccac ccaccacctt aagagtaggg agaagatctt cttactcttt
     5821 tttttttttt ttttttttgc tgtttgttta ctcgttctcc ttcctgcccc tgctcccaga
     5881 ctcttggtcc cccaccactg cccccccttt tctcccactc ccaggcccat ttcctttttt
     5941 tttttttctt ttcttttctg gagtgtgtgg tgaaacagaa aaatcatgtt taataaacgg
     6001 agattgttct ttta
//
