LOCUS       NM_001290565            1079 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus spectrin repeat containing, nuclear envelope family
            member 4 (Syne4), transcript variant 2, mRNA.
ACCESSION   NM_001290565 XM_006539884
VERSION     NM_001290565.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Taiber,S., Gozlan,O., Cohen,R., Andrade,L.R., Gregory,E.F.,
            Starr,D.A., Moran,Y., Hipp,R., Kelley,M.W., Manor,U., Sprinzak,D.
            and Avraham,K.B.
  TITLE     A Nesprin-4/kinesin-1 cargo model for nuclear positioning in
            cochlear outer hair cells
  JOURNAL   Front Cell Dev Biol 10, 974168 (2022)
   PUBMED   36211453
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Taiber,S., Cohen,R., Yizhar-Barnea,O., Sprinzak,D., Holt,J.R. and
            Avraham,K.B.
  TITLE     Neonatal AAV gene therapy rescues hearing in a mouse model of SYNE4
            deafness
  JOURNAL   EMBO Mol Med 13 (2), e13259 (2021)
   PUBMED   33350593
REFERENCE   3  (bases 1 to 1079)
  AUTHORS   Feng,Q. and Kornmann,B.
  TITLE     Mechanical forces on cellular organelles
  JOURNAL   J Cell Sci 131 (21) (2018)
   PUBMED   30373893
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1079)
  AUTHORS   Horn,H.F., Brownstein,Z., Lenz,D.R., Shivatzki,S., Dror,A.A.,
            Dagan-Rosenfeld,O., Friedman,L.M., Roux,K.J., Kozlov,S.,
            Jeang,K.T., Frydman,M., Burke,B., Stewart,C.L. and Avraham,K.B.
  TITLE     The LINC complex is essential for hearing
  JOURNAL   J Clin Invest 123 (2), 740-750 (2013)
   PUBMED   23348741
REFERENCE   5  (bases 1 to 1079)
  AUTHORS   Morimoto,A., Shibuya,H., Zhu,X., Kim,J., Ishiguro,K., Han,M. and
            Watanabe,Y.
  TITLE     A conserved KASH domain protein associates with telomeres, SUN1,
            and dynactin during mammalian meiosis
  JOURNAL   J Cell Biol 198 (2), 165-172 (2012)
   PUBMED   22826121
REFERENCE   6  (bases 1 to 1079)
  AUTHORS   Roux,K.J., Crisp,M.L., Liu,Q., Kim,D., Kozlov,S., Stewart,C.L. and
            Burke,B.
  TITLE     Nesprin 4 is an outer nuclear membrane protein that can induce
            kinesin-mediated cell polarization
  JOURNAL   Proc Natl Acad Sci U S A 106 (7), 2194-2199 (2009)
   PUBMED   19164528
  REMARK    GeneRIF: Nesprin 4 is an outer nuclear membrane protein that can
            induce kinesin-mediated cell polarization.
REFERENCE   7  (bases 1 to 1079)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC149067.3.
            
            On Dec 30, 2022 this sequence version replaced NM_001290565.1.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (2) has the same
            N- and C-termini but is shorter compared to isoform 1.
            
            Sequence Note:.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR15390195.615918.1,
                                           SRR15390196.132631.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-244               AC149067.3         123542-123785
            245-383             AC149067.3         123961-124099
            384-527             AC149067.3         124929-125072
            528-722             AC149067.3         125272-125466
            723-797             AC149067.3         126667-126741
            798-856             AC149067.3         127372-127430
            857-1079            AC149067.3         127523-127745
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 17.39 cM"
     gene            1..1079
                     /gene="Syne4"
                     /gene_synonym="0610012K07Rik; KASH4; Nesp4"
                     /note="spectrin repeat containing, nuclear envelope family
                     member 4"
                     /db_xref="GeneID:233066"
                     /db_xref="MGI:MGI:2141950"
     exon            1..244
                     /gene="Syne4"
                     /gene_synonym="0610012K07Rik; KASH4; Nesp4"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..1034
                     /gene="Syne4"
                     /gene_synonym="0610012K07Rik; KASH4; Nesp4"
                     /note="isoform 2 is encoded by transcript variant 2;
                     nesprin-4; nuclear envelope spectrin repeat protein 4;
                     KASH domain-containing protein 4"
                     /codon_start=1
                     /product="nesprin-4 isoform 2"
                     /protein_id="NP_001277494.1"
                     /db_xref="CCDS:CCDS71929.1"
                     /db_xref="GeneID:233066"
                     /db_xref="MGI:MGI:2141950"
                     /translation="MALVPPLGREFPPEPVNCPLAAPRELDVVGGTICPAPEEETSRP
                     EQVQASLGLPEHCMGELKSTESATSPSRLPLASSHEHQDGGKPCEHSDSGLEVLEAEQ
                     DSLHLCLLRLNFRLQDLERGLGSWTLAHNRIVQMQALQAELRGAAERVDALLAFGEGL
                     AERSEPRAWASLEQVLRALGTHRDTIFQRLWQLQAQLISYSLDLLSLGLGHRKHLAAH
                     HRRRLRKPQDRKRQVSPSLPDAMLEVDRGVPAPASKRPLTLFFLLLFLLLVGATLLLP
                     LSGVSCCSHARLARTPYLVLSYVNGLPPI"
     exon            245..383
                     /gene="Syne4"
                     /gene_synonym="0610012K07Rik; KASH4; Nesp4"
                     /inference="alignment:Splign:2.1.0"
     exon            384..527
                     /gene="Syne4"
                     /gene_synonym="0610012K07Rik; KASH4; Nesp4"
                     /inference="alignment:Splign:2.1.0"
     exon            528..722
                     /gene="Syne4"
                     /gene_synonym="0610012K07Rik; KASH4; Nesp4"
                     /inference="alignment:Splign:2.1.0"
     exon            723..797
                     /gene="Syne4"
                     /gene_synonym="0610012K07Rik; KASH4; Nesp4"
                     /inference="alignment:Splign:2.1.0"
     exon            798..856
                     /gene="Syne4"
                     /gene_synonym="0610012K07Rik; KASH4; Nesp4"
                     /inference="alignment:Splign:2.1.0"
     exon            857..1079
                     /gene="Syne4"
                     /gene_synonym="0610012K07Rik; KASH4; Nesp4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1057..1062
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Syne4"
                     /gene_synonym="0610012K07Rik; KASH4; Nesp4"
                     /note="hexamer: ATTAAA"
     polyA_site      1075
                     /gene="Syne4"
                     /gene_synonym="0610012K07Rik; KASH4; Nesp4"
                     /note="major polyA site"
ORIGIN      
        1 agtagcctcg gacttaggct gtgagtcctg ctgagaaagc atgggggtct ggactgtcta
       61 agaaagcggc tgctgttact ggacatcctc acctgacgat ccccaagtgc acggccatgg
      121 ccctggttcc acctcttggc cgtgaattcc cgccagagcc tgtgaattgt cccctggcag
      181 ctcctagaga gctggatgtt gtcggaggga ccatctgccc tgcacctgag gaagagacaa
      241 gcaggccaga gcaggtccag gcctccttgg gcctgcctga gcattgcatg ggtgaactga
      301 agagcactga gtctgccacc agcccctcaa gactccccct ggcctcttcc catgagcatc
      361 aagacggggg caagccctgt gagcactctg actctggttt ggaagtacta gaagctgaac
      421 aggacagtct acatctttgt ctgttgaggt tgaacttccg gctgcaggac ctggaacgag
      481 gccttgggtc ttggacgctg gcccacaaca ggattgtcca gatgcaggcc ctgcaggcag
      541 agctacgagg ggccgctgag cgcgtggatg cattgcttgc atttggtgag ggcctggcag
      601 agaggagtga gcccagggcc tgggcatccc tggagcaggt cctgagggcc cttggaaccc
      661 accgagacac catcttccaa cggctctggc agctgcaggc ccagttgatc agctatagcc
      721 tggacctgct ctccttgggg cttggccacc ggaaacattt agcggctcac catcgaaggc
      781 ggctccggaa gcctcaggac aggaagaggc aagtatcccc cagtctgcct gatgcaatgc
      841 tggaagtgga tcgcggggtc ccagctcctg catccaagcg gcccctgacc ctcttctttc
      901 tccttctctt cctccttctg gtgggtgcca cgctgttgct gcccttgtcg ggggtctcct
      961 gctgttctca tgcccggctg gctaggacgc cctacctggt gctcagttat gtcaatggtc
     1021 tccctccaat ctgagcccac catccaagac acgtgcatta aatggtcact ttcaagaga
//
