LOCUS       NM_001042699            5473 bp    mRNA    linear   ROD 08-FEB-2025
DEFINITION  Mus musculus spectrin repeat containing, nuclear envelope family
            member 3 (Syne3), transcript variant 1, mRNA.
ACCESSION   NM_001042699 XM_983651
VERSION     NM_001042699.3
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Liao,L., Zhang,L., Yang,M., Wang,X., Huang,W., Wu,X., Pan,H.,
            Yuan,L., Huang,W., Wu,Y. and Guan,J.
  TITLE     Expression profile of SYNE3 and bioinformatic analysis of its
            prognostic value and functions in tumors
  JOURNAL   J Transl Med 18 (1), 355 (2020)
   PUBMED   32948197
  REMARK    GeneRIF: Expression profile of SYNE3 and bioinformatic analysis of
            its prognostic value and functions in tumors.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5473)
  AUTHORS   Rapisarda,V., Malashchuk,I., Asamaowei,I.E., Poterlowicz,K.,
            Fessing,M.Y., Sharov,A.A., Karakesisoglou,I., Botchkarev,V.A. and
            Mardaryev,A.
  TITLE     p63 Transcription Factor Regulates Nuclear Shape and Expression of
            Nuclear Envelope-Associated Genes in Epidermal Keratinocytes
  JOURNAL   J Invest Dermatol 137 (10), 2157-2167 (2017)
   PUBMED   28595999
REFERENCE   4  (bases 1 to 5473)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 5473)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 5473)
  AUTHORS   Young,K.G. and Kothary,R.
  TITLE     Dystonin/Bpag1 is a necessary endoplasmic reticulum/nuclear
            envelope protein in sensory neurons
  JOURNAL   Exp Cell Res 314 (15), 2750-2761 (2008)
   PUBMED   18638474
REFERENCE   7  (bases 1 to 5473)
  AUTHORS   Stewart-Hutchinson,P.J., Hale,C.M., Wirtz,D. and Hodzic,D.
  TITLE     Structural requirements for the assembly of LINC complexes and
            their function in cellular mechanical stiffness
  JOURNAL   Exp Cell Res 314 (8), 1892-1905 (2008)
   PUBMED   18396275
REFERENCE   8  (bases 1 to 5473)
  AUTHORS   Ketema,M., Wilhelmsen,K., Kuikman,I., Janssen,H., Hodzic,D. and
            Sonnenberg,A.
  TITLE     Requirements for the localization of nesprin-3 at the nuclear
            envelope and its interaction with plectin
  JOURNAL   J Cell Sci 120 (Pt 19), 3384-3394 (2007)
   PUBMED   17881500
  REMARK    GeneRIF: Data support a model in which the actin cytoskeleton
            influences the binding of plectin dimers to dimers of
            nesprin-3alpha, which in turn are retained at the nuclear envelope
            through an interaction with Sun proteins.
REFERENCE   9  (bases 1 to 5473)
  AUTHORS   Wilhelmsen,K., Litjens,S.H., Kuikman,I., Tshimbalanga,N.,
            Janssen,H., van den Bout,I., Raymond,K. and Sonnenberg,A.
  TITLE     Nesprin-3, a novel outer nuclear membrane protein, associates with
            the cytoskeletal linker protein plectin
  JOURNAL   J Cell Biol 171 (5), 799-810 (2005)
   PUBMED   16330710
  REMARK    GeneRIF: plectin binds to the integrin alpha6beta4 at the cell
            surface and to nesprin-3 at the ONM in keratinocytes.
REFERENCE   10 (bases 1 to 5473)
  AUTHORS   Starr,D.A. and Fischer,J.A.
  TITLE     KASH 'n Karry: the KASH domain family of cargo-specific
            cytoskeletal adaptor proteins
  JOURNAL   Bioessays 27 (11), 1136-1146 (2005)
   PUBMED   16237665
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140477.2 and AC147375.3.
            
            On Mar 30, 2022 this sequence version replaced NM_001042699.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC099694.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN00849374,
                                           SAMN00849375 [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-171               AC140477.2         47458-47628         c
            172-303             AC140477.2         46397-46528         c
            304-461             AC140477.2         24664-24821         c
            462-634             AC140477.2         18127-18299         c
            635-944             AC140477.2         16808-17117         c
            945-1106            AC140477.2         12097-12258         c
            1107-1207           AC140477.2         10499-10599         c
            1208-1454           AC147375.3         176264-176510       c
            1455-1588           AC147375.3         174335-174468       c
            1589-1763           AC147375.3         172892-173066       c
            1764-1946           AC147375.3         170465-170647       c
            1947-2090           AC147375.3         169101-169244       c
            2091-2225           AC147375.3         167924-168058       c
            2226-2387           AC147375.3         161830-161991       c
            2388-2560           AC147375.3         161504-161676       c
            2561-2690           AC147375.3         160019-160148       c
            2691-2849           AC147375.3         158243-158401       c
            2850-2999           AC147375.3         155544-155693       c
            3000-3038           AC147375.3         154520-154558       c
            3039-5473           AC147375.3         144875-147309       c
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 54.97 cM"
     gene            1..5473
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /note="spectrin repeat containing, nuclear envelope family
                     member 3"
                     /db_xref="GeneID:212073"
                     /db_xref="MGI:MGI:2442408"
     exon            1..171
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            172..303
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            304..461
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     CDS             318..3245
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /note="isoform alpha is encoded by transcript variant 1;
                     nuclear envelope spectrin repeat protein 3; KASH
                     domain-containing protein 3"
                     /codon_start=1
                     /product="nesprin-3 isoform alpha"
                     /protein_id="NP_001036164.1"
                     /db_xref="CCDS:CCDS36542.1"
                     /db_xref="GeneID:212073"
                     /db_xref="MGI:MGI:2442408"
                     /translation="MTQQPQEDFERSVEDAQAWMKVIQEQLQVNDNTKGPRAALEARL
                     RETEKICQLESEGMVKVELVLRAAEALLATCQEGQKPEILARLRDIKSQWEETVTYMT
                     HCHSRIEWVWLHWSEYLLAQDEFYRWFQKMVVALEPPVELQLGLKEKQWQLSHAQVLL
                     HNVDNQAVLLDRLLEEAGSLFSRIGDPSVDEDAQKRMKAEYDAVKARAQRRVDLLAQV
                     AQDHEQYREDVNEFQLWLKAVVEKVHSCLGRNCKLATELRLSTLQDIAKDFPRGEESL
                     KRLEEQAVGVIQNTSPLGAEKISGELEEMRGVLEKLRVLWKEEEGRLRGLLQSRGDCE
                     QQIQQLEAELGDFKKSLQRLAQEGLEPTVKTATEDELVAQWRLFSGTRAALASEEPRV
                     DRLQTQLKKLVTFPDLQSLSDSVVATIQEYQSMKGKNTRLHNATRAELWQRFQRPLND
                     LQLWKALAQRLLDITASLPDLASIHTFLPQIEAALTESSRLKEQLAMLQLKTDLLGSI
                     FGQERAATLLEQVTSSVRDRDLLHNSLLQRKSKLQSLLVQHKDFGVAFDPLNRKLLDL
                     QARIQAEKGLPRDLPGKQVQLLRLQGLQEEGLDLGTQIEAVRPLAHGNSKHQQKVDQI
                     SCDQQALQRSLEDLVDRCQQNVREHCTFSHRLSELQLWITMATQTLESHQGDVRLWDA
                     ESQEAGLETLLSEIPEKEVQVSLLQALGQLVMKKSSPEGATMVQEELRKLMESWQALR
                     LLEENMLSLMRNQQLQRTEVDTGKKQVFTNNIPKAGFLINPQDPIPRRQHGANPLEGH
                     DLPEDHPQLLRDFEQWLQAENSKLRRIITMRVATAKDLRTREVKLQELEARIPEGQHL
                     FENLLRLRPARDPSNELEDLRYRWMLYKSKLKDSGHLLTESSPGELTAFQKSRRQKRW
                     SPCSLLQKACRVALPLQLLLLLFLLLLFLLPAGEEERSCALANNFARSFALMLRYNGP
                     PPT"
     exon            462..634
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            635..944
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1106
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1454
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1588
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1763
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1946
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2090
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2091..2225
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2226..2387
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2388..2560
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2561..2690
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2691..2849
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2850..2999
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3000..3038
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3039..5473
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5445..5450
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /note="hexamer: AATATA"
     polyA_site      5473
                     /gene="Syne3"
                     /gene_synonym="KASH3; nesprin-3"
                     /note="major polyA site"
ORIGIN      
        1 ccttcctgct gtgcctggca gtcttggagg accagcattg ttgaggggtc ccacagctag
       61 gcgtggctcc agactgaggg cgtatcttca catagagttc caatttagag actccagcct
      121 acagacaaac atttgggaag gatcatgttt ttctgagttt cttctggaca ggtggaagct
      181 ttcttatcct ggggccaacc ctgcctgtct ggaaccctgt gcttaataat tcctgtccct
      241 gtggatgtgt gggctggaga gtcttccttg tttcctccgt gctggagaca agcgaccggg
      301 aagccctcgc aggtgcgatg acgcagcagc cccaggagga ctttgagagg agcgtggagg
      361 atgcccaggc ctggatgaag gtgatacagg agcagcttca ggtcaatgac aacacgaagg
      421 ggccccgagc ggccctggag gcaaggcttc gagagacaga gaaaatctgc cagctggagt
      481 ctgaaggaat ggtgaaggtg gaactggtcc tgcgggcggc ggaggccctc ttggcaactt
      541 gccaggaggg ccagaaacct gagatcctgg cccggctgag ggatatcaag tctcagtggg
      601 aggagacggt cacctacatg acccactgcc acagtcgcat cgagtgggtg tggctgcact
      661 ggagtgagta cctgctggcc caggatgagt tttaccgctg gttccagaag atggtggtcg
      721 cactggagcc ccccgtggag ctgcagctgg gcttgaagga gaagcaatgg cagctgagcc
      781 acgcccaggt gctgctgcac aacgtggaca atcaggctgt gctcctggac aggctgttgg
      841 aggaggcggg ctccctgttc agcaggatcg gagaccccag cgtggatgaa gatgcccaga
      901 agaggatgaa ggctgagtac gatgccgtga aggccagagc ccagcgcagg gtggacctcc
      961 tggcccaggt ggcccaggac catgagcagt accgggagga cgtgaatgag ttccagctgt
     1021 ggctgaaggc ggtggtggag aaggtgcaca gctgtctggg gcggaactgc aagctggcca
     1081 cagaacttcg tctctctacg ctgcaggaca tcgccaagga tttccctagg ggtgaggagt
     1141 ctctgaaaag attggaggaa caggctgtgg gtgtcattca aaacacctct cccttgggtg
     1201 cagagaagat ctcaggggag ctggaggaga tgcggggtgt cctggagaag ctgagagtcc
     1261 tctggaaaga ggaggaaggg aggctgcggg gcctgctcca gtccaggggg gactgtgagc
     1321 agcagatcca acagctggag gcagaactgg gagacttcaa gaaaagcctt cagaggctgg
     1381 cccaggaggg cttggagccc acggtgaaga cagccacaga ggatgagctg gtggcccagt
     1441 ggaggctgtt ctcggggact cgggctgcac tggcttcaga ggaaccccgt gtagaccggt
     1501 tacaaactca actgaagaaa cttgtcacct tcccggacct gcagtcactc tctgacagcg
     1561 tggtagccac cattcaggaa taccaaagta tgaaggggaa gaataccagg ctccacaatg
     1621 cgacccgggc agagctgtgg cagcgtttcc agcggcccct aaatgacctg cagctgtgga
     1681 aggccctggc ccagaggctc ctggacatca cggccagcct gcctgacctg gcctccattc
     1741 acacctttct accccagatt gaggcggccc tcacggaaag ctctcgcctg aaagagcagc
     1801 tggcgatgct gcagctgaag accgacctgc tgggcagcat ctttggccag gagagagcag
     1861 ccaccctcct ggagcaggtg acaagttctg tgagggacag agacctactg cataacagcc
     1921 ttcttcagcg gaagagcaaa cttcagagcc tgcttgttca gcacaaggac tttggggtgg
     1981 cttttgatcc cctaaacagg aagctcctag acctccaggc caggatccaa gcagagaaag
     2041 ggcttccgag ggaccttcct ggaaagcagg tccagctcct aaggttgcag gggctgcagg
     2101 aagaggggct ggatctgggg acacagatcg aggctgtgag gcctcttgcc catgggaact
     2161 ctaagcacca gcagaaagta gaccagatct cctgtgacca gcaagccctg cagaggtccc
     2221 tggaggatct cgtggacagg tgtcagcaga acgtacggga acattgtacc ttcagtcaca
     2281 ggctgtcgga gctgcagcta tggatcacca tggccacaca gacattagag tcacaccaag
     2341 gggatgtgcg tctgtgggat gctgagtccc aagaggctgg actcgagacg ctgctgtctg
     2401 aaatcccaga gaaagaggtc caggtgtccc tgctccaagc actgggccag cttgtgatga
     2461 agaagtcttc cccagaaggg gcaaccatgg tccaggagga gctgaggaag ctgatggagt
     2521 cttggcaggc cctgcggctg ctagaggaga acatgctgag tctcatgaga aaccagcagc
     2581 tgcagaggac agaggtggac acggggaaga agcaggtgtt caccaacaac atcccaaagg
     2641 ccggctttct catcaaccct caggacccca ttcccaggag acagcatggg gcaaacccac
     2701 tggaaggaca cgacctccct gaagatcatc cccagctcct gagggacttt gaacagtggc
     2761 tgcaggcaga aaactccaag ctacgtagaa tcatcacaat gagagtggcc acagccaagg
     2821 acttgaggac cagagaggtg aagctgcagg agctggaggc ccgaatccca gaaggccagc
     2881 acctctttga gaacctgctt cgtctcaggc cggcaaggga cccctccaac gagctggaag
     2941 atctgcgcta ccggtggatg ctgtacaagt ccaagctcaa ggactctggc cacctgctga
     3001 ccgagagttc tccgggggag ctgactgcat tccagaagag tcggaggcag aagcggtgga
     3061 gtccctgctc tctcctacag aaagcatgcc gtgtggcact gccattgcag ctgttgctcc
     3121 tgctctttct gctgctgctg ttcctgctgc cggccggcga ggaggagcgc agctgcgccc
     3181 tggccaacaa cttcgcccgc tcctttgcgc tcatgcttcg gtacaatggc cccccgccca
     3241 cctagcccac tgggcgcaca ggtgaccttc tgcagtccct cagaggctgg ctgctggggg
     3301 cccagtagcc gatctcacac ccagagcagc cttagaccaa atgctgcctc tgtttccaga
     3361 acaaacttgc ctttttctac gatgctgctt ctgtctattt atactaaaat gtgtactgtg
     3421 tgtggttagg gaatatgtac agaattttta tatgccatgt ctgtacatgc tcagtgtaga
     3481 taacttcaga tacaatgtct ctttgtgcct atggaagccc aaaacagtgg gtttctctag
     3541 tgggatttgt ggcacttgtg ccttagcctc ctggaatccc tgggggagag ggaaaaaaaa
     3601 aaatcacccg ttactttgat gacagcctgt taaccagaaa tccagatgtt gctgaagaag
     3661 cctctgagtg gttaccatag ccacacagaa ttggagtcct gggacttgag tatttggcta
     3721 gttttaaagt cacacagact tgggccgtgg gctagataaa gagaagccgt gatggtgggc
     3781 cactggctta gccagagcag tgagacaaga tgcgtgccag agggatgcca agagagcaac
     3841 aagaagagct gtaaagatgg agcccaatgg agcaccacaa aatgatggta cggagcaccc
     3901 aaccctgcac agcagccact gataacacag accatggagg aatgcccatg actatgactg
     3961 tgaccgtgac cgtgaccatg accgtgactg cgatcccacg tcttcagaag tggctgggtt
     4021 ttatttgcat gttttctcca ggagaaagaa aaagatgaac ttgacttttt attctcatgc
     4081 ataagggtga agtggcctgt gtcagtcctg ggaggcccgt gccactgtac acagctgttg
     4141 agctgtctgc agcctagggc atgagctgag agcagagtgg aaatgcttgc ccaccaggcc
     4201 aggaggtcca ccctaagcag agaggagaaa ctcaggccat ctgaaggggc ctctcttccc
     4261 ttccaagaag ggactgtgca ggctggtggg tctacaaggc tgggcaggtc ccacattccc
     4321 ccatgggcct acggctcaca gtctgacccc aggcaggagc aggcaaaggc agctccaggc
     4381 acaggtggga ggcatctggg tgattccagg ttatgcccaa ggctggacac ataaccaggg
     4441 aattctggga tgattcctaa gtgctttaaa actggatcgg gttcacccta aaccgacact
     4501 ttttttttta acctttagaa aatacttaac actccctttt tccagatggg attacacact
     4561 cctttggagt gtgtgtgctc gcaggcctga tcaccagctc accatcatca gtgtcacaga
     4621 cccagagagg cactttaaat acagcaaata ctgtagccct aagagggact ggactcccag
     4681 gtcaccgcac tgatgcacat gcaagctggc atcaggctgt atcagaggca gctttcatct
     4741 tagcatggta cccagaaaag gttggacatc agcaaacagg aaagcagtgt cttggggctc
     4801 gtgaccctga ctttgtctgt aaccccagtc acaaagccct ctctgcacct ggccactttg
     4861 ctgggaaatc agggactggt gaaccctgac ctcacttaga cccaggtagc cgagccctga
     4921 tctcagaccc agatagcaga gccctgatgc tcactggcca agaaagacca ctggtccacc
     4981 actccatcag aactgtggca agggccatca atacttctgt aaccactttt ccatttcaaa
     5041 agtaaaaaac ctgaggccca gagaggggaa gtaactcacc catagccaca cagcaagaac
     5101 ctatacagcc agaaatggac tgataccagg tttattagag caaacctccc cacattttcc
     5161 ttcccaggaa aaatgaacag ggctcgactt tggagcactt gcctaacagg tgtgagtcct
     5221 gagttccatg acctgaattg aagagggatt gggggagagc tgtttgctac gaatgtgggc
     5281 gtgtctcctg ataaatactg gggagggtca aacttcaata cctgggtggg ggtggggcag
     5341 ggtgggagcc tggctgtgtt tttaacgaca atgacactgt ggattgcttg ttgtgtaaca
     5401 ttttttttaa cagaggggac tgatattttt attactttca tatgaatata gctgcaatat
     5461 gacgtgctag cca
//
