LOCUS       NM_153399               6982 bp    mRNA    linear   ROD 17-DEC-2022
DEFINITION  Mus musculus spectrin repeat containing, nuclear envelope 1
            (Syne1), transcript variant 1, mRNA.
ACCESSION   NM_153399 XM_358299
VERSION     NM_153399.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Sankoda N, Tanabe W, Tanaka A, Shibata H, Woltjen K, Chiba T, Haga
            H, Sakai Y, Mandai M, Yamamoto T, Yamada Y, Uemoto S and Kawaguchi
            Y.
  TITLE     Epithelial expression of Gata4 and Sox2 regulates specification of
            the squamous-columnar junction via MAPK/ERK signaling in mice
  JOURNAL   Nat Commun 12 (1), 560 (2021)
   PUBMED   33495473
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Levy Y, Ross JA, Niglas M, Snetkov VA, Lynham S, Liao CY,
            Puckelwartz MJ, Hsu YM, McNally EM, Alsheimer M, Harridge SD, Young
            SG, Fong LG, Espanol Y, Lopez-Otin C, Kennedy BK, Lowe DA and
            Ochala J.
  TITLE     Prelamin A causes aberrant myonuclear arrangement and results in
            muscle fiber weakness
  JOURNAL   JCI Insight 3 (19), 120920 (2018)
   PUBMED   30282816
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6982)
  AUTHORS   Potter C, Razafsky D, Wozniak D, Casey M, Penrose S, Ge X, Mahjoub
            MR and Hodzic D.
  TITLE     The KASH-containing isoform of Nesprin1 giant associates with
            ciliary rootlets of ependymal cells
  JOURNAL   Neurobiol Dis 115, 82-91 (2018)
   PUBMED   29630990
REFERENCE   4  (bases 1 to 6982)
  AUTHORS   Gimpel P, Lee YL, Sobota RM, Calvi A, Koullourou V, Patel R,
            Mamchaoui K, Nedelec F, Shackleton S, Schmoranzer J, Burke B, Cadot
            B and Gomes ER.
  TITLE     Nesprin-1alpha-Dependent Microtubule Nucleation from the Nuclear
            Envelope via Akap450 Is Necessary for Nuclear Positioning in Muscle
            Cells
  JOURNAL   Curr Biol 27 (19), 2999-3009 (2017)
   PUBMED   28966089
  REMARK    GeneRIF: Nesprin-1alpha recruits Akap450 to the nuclear envelope
            (NE)independently of kinesin and that Akap450, but not other
            centrosomal proteins, is required for microtubule nucleation from
            the NE.
REFERENCE   5  (bases 1 to 6982)
  AUTHORS   Potter C, Zhu W, Razafsky D, Ruzycki P, Kolesnikov AV, Doggett T,
            Kefalov VJ, Betleja E, Mahjoub MR and Hodzic D.
  TITLE     Multiple Isoforms of Nesprin1 Are Integral Components of Ciliary
            Rootlets
  JOURNAL   Curr Biol 27 (13), 2014-2022 (2017)
   PUBMED   28625779
  REMARK    GeneRIF: In agreement with the association of Nesprin1alpha with
            photoreceptor ciliary rootlets and the functional interaction
            between rootletin and Nesprin1 in fibroblasts, the authors
            demonstrate that multiple isoforms of Nesprin1 are integral
            components of ciliary rootlets of multiciliated ependymal and
            tracheal cells.
REFERENCE   6  (bases 1 to 6982)
  AUTHORS   Padmakumar VC, Abraham S, Braune S, Noegel AA, Tunggal B,
            Karakesisoglou I and Korenbaum E.
  TITLE     Enaptin, a giant actin-binding protein, is an element of the
            nuclear membrane and the actin cytoskeleton
  JOURNAL   Exp Cell Res 295 (2), 330-339 (2004)
   PUBMED   15093733
REFERENCE   7  (bases 1 to 6982)
  AUTHORS   Okazaki N, Kikuno R, Ohara R, Inamoto S, Aizawa H, Yuasa S,
            Nakajima D, Nagase T, Ohara O and Koga H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   8  (bases 1 to 6982)
  AUTHORS   Zhang Q, Ragnauth C, Greener MJ, Shanahan CM and Roberts RG.
  TITLE     The nesprins are giant actin-binding proteins, orthologous to
            Drosophila melanogaster muscle protein MSP-300
  JOURNAL   Genomics 80 (5), 473-481 (2002)
   PUBMED   12408964
REFERENCE   9  (bases 1 to 6982)
  AUTHORS   Zhang Q, Skepper JN, Yang F, Davies JD, Hegyi L, Roberts RG,
            Weissberg PL, Ellis JA and Shanahan CM.
  TITLE     Nesprins: a novel family of spectrin-repeat-containing proteins
            that localize to the nuclear membrane in multiple tissues
  JOURNAL   J Cell Sci 114 (Pt 24), 4485-4498 (2001)
   PUBMED   11792814
REFERENCE   10 (bases 1 to 6982)
  AUTHORS   Apel ED, Lewis RM, Grady RM and Sanes JR.
  TITLE     Syne-1, a dystrophin- and Klarsicht-related protein associated with
            synaptic nuclei at the neuromuscular junction
  JOURNAL   J Biol Chem 275 (41), 31986-31995 (2000)
   PUBMED   10878022
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC157020.8, AK044459.1, AK039486.1 and AK122550.3.
            
            [WARNING] On Apr 20, 2023 this sequence was replaced by
            NM_153399.3.
            
            On Dec 8, 2006 this sequence version replaced NM_153399.1.
            
            Transcript Variant: This variant (1) differs in both UTRs and has
            multiple differences in the coding region compared to variant 4.
            These differences result in the use of an alternate start codon,
            compared to variant 4. The encoded isoform (1) is shorter and has
            distinct N- and C-termini compared to isoform 4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF535143.1, SRR9219382.63468.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-135               AC157020.8         4275-4409           c
            136-946             AK044459.1         123-933
            947-2299            AK039486.1         1377-2729
            2300-6982           AK122550.3         730-5412
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10"
     gene            1..6982
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /note="spectrin repeat containing, nuclear envelope 1"
                     /db_xref="GeneID:64009"
                     /db_xref="MGI:MGI:1927152"
     exon            1..234
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            235..499
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     CDS             433..4728
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /note="isoform 1 is encoded by transcript variant 1; KASH
                     domain-containing protein 1; nesprin 1; enaptin; myocyte
                     nuclear envelope protein 1; synaptic nuclear envelope
                     protein 1; synaptic nuclear envelope 1; nuclear envelope
                     spectrin repeat protein 1; enaptin165"
                     /codon_start=1
                     /product="nesprin-1 isoform 1"
                     /protein_id="NP_700448.2"
                     /db_xref="CCDS:CCDS56680.1"
                     /db_xref="GeneID:64009"
                     /db_xref="MGI:MGI:1927152"
                     /translation="MATSRASSRSHRDITNVMQRLQDEQEIVQKRTFTKWINSHLAKR
                     KPPMVVDDLFEDMKDGIKLLALLEVLSGQKLPCEQGHRVKRIHAVANIGTALKFLEGR
                     KSMYRGSPIKLVNINATDIADGRPSIVLGLMWTIILYFQIEELTSNLPQLQSLSSSAS
                     SVDSMVSTETASPPSKRKVAAKIQGNAKKTLLKWVQHTAGKQMGIEVKDFGKSWRTGL
                     AFHSVIHAIQPELVDLEKVKTRSNRENLEDAFTIAETQLGIPRLLDPEDVDVDKPDEK
                     SIMTYVAQFLTQYPDIHGAGCDGQEDDRDDRLILKETKVWIEQFERDFTRAQMTESSL
                     QDKYQAFKHFRVQYEMKRKQVEHIIQPLQRDGKLTLDQALVKQCWERVSSRLFDWHIQ
                     LDKSLPAPLGTIGAWLYRAEVALREEITIQQVHEETANTIQRKLEQHKDLLQNTDAHK
                     RAFHEIYQTRSVNGIPMPPDQLEDMAERFHFVSSTSELHLMKMEFLELKYRLLSLLVL
                     AESKLKSWIIKYGRRESVELLLQSYISFIENSKFFEQYEVTYQILKQTADIYVKAEGS
                     VEEAENVMKFMSEATAQWRNLSVEVRSVRSMLEEVISNWDRYGDTVASLQAWLEDAEK
                     MLSQSEHAKKDFFRNLPHWIQQHTAMNDAGNFLIETCDEIVSRDLKQQLLLLNGRWRE
                     LFMEVKQYARADEMDRMKKEYIDVTTTLFGFATEAHRKLSEPLEVSFINVKLLIQDLE
                     DLEKRVPVMDAQYKMIAKKAHLFAKESPQEEANEMLTTMSKLKEQLSKVKECCSPLLY
                     EAQQLTVPLEELETQITSFYDSLGKINEILSVLEQEAQSSTLFKQKHQELLASQENCK
                     KSLTLIEKGSQSVQKLVTSSQARKPWDHTKLQKQIADVHHAFQSMIKKTGDWKKHVEA
                     NSRLMKKFEESRAELEKVLRVAQEGLEEKGDPEELLRRHTEFFSQLDQRVLNAFLKAC
                     DELTDILPEQEQQGLQEAVRKLHKQWKDLQGEAPYHLLHLKIAVEKDRFSAAVEECRA
                     ELEQETKLAPQEGSEKIIKEHRVFFSDKGPHHLCEKRLQLIEELCGKLPVQDPVRDTC
                     GACHTALKELKASIDNTYTMLVDDPDKWKDYTSRFSEFSSWVSAKKACLKKIKDEPID
                     TGNHDEVKHMVDEIRNDITKKGESLSWLKSRLKYLIDISSENEAQKRGDELAELSSSF
                     KALVALLSEVEKLLSNFGECVQYKEIVKSSLEGLISGPQESKEEAEMILDSKNLLEAQ
                     QLLLHHQQKTKMISAKKRDLQEQMEQAQQGGQAGPGQEELRKLESTLTGLEQSRERQE
                     RRIQVSLRKWERFETNKETVVRYLFQTGSSHERFLSFSSLESLSSELEQTKEFSKRTE
                     SIATQAENLVKEAAELPLGPRNKRVLQRQAKSIKEQVTTLEDTLEEEYVLHHF"
     exon            500..561
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            562..657
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            658..741
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            742..762
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            763..855
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            856..1034
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1231
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1341
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1449
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1587
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1752
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..1865
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..2034
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..2131
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2334
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            2335..2499
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            2500..2652
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            2653..2796
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            2797..2970
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            2971..3129
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            3130..3294
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            3295..3429
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            3430..3588
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            3589..3797
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            3798..3906
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            3907..4071
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            4072..4239
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            4240..4404
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            4405..4545
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
     exon            4546..6982
                     /gene="Syne1"
                     /gene_synonym="8B; A330049M09Rik; C130039F11Rik; CPG2;
                     KASH1; mKIAA1756; myne-1; Myne1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccagcagcca gcgcagagca gcgcgtgtgc gccaacccca tcaccggccc acaccgggct
       61 gggattgttg tactctgtca ccagggcatc ctggctcccg cagccctgcg gacagcgccg
      121 gagaccgggc tactaagcca gggctgccgc gcacagccgg agcggcacgc agccgggaag
      181 aggcgttcgc tagcgtcacc tcactgcttg gaaccagagc aaggccggat caaggttcct
      241 gagtctactt aagaagaaaa ggaggagagc atgcagttca caggaattac agcgtctcct
      301 cactgatcac cacgagtgcc tgagatttgt ctgagactca atgggtatac acaagacagt
      361 gtatgttatc tgaagcagca taatttcccc tgcactgaga gacttcactt ggtgctggtt
      421 ccatgctgga ccatggcaac ctccagagca tcttcccggt cccatcggga catcaccaat
      481 gtgatgcaga ggctacaaga tgaacaagag attgtacaga aacgtacctt cacaaaatgg
      541 atcaattccc atctggccaa gcggaagcct cccatggtgg tggatgacct ttttgaagac
      601 atgaaagatg gcatcaagct gcttgcttta ctggaggtcc tgtctgggca gaaactgcct
      661 tgtgaacaag gacaccgggt gaagcgtatt catgctgtgg ctaacattgg caccgcactc
      721 aaattccttg aaggaaggaa gtccatgtac agaggatcac cgattaaatt agtcaacatt
      781 aatgctactg atattgctga tggccgacca tcaatcgttc ttgggctgat gtggaccatt
      841 atcctgtatt tccagataga agagttgacc agcaacctac cacagctgca gtctctatcc
      901 agcagtgctt cctctgtgga cagcatggtc agcaccgaga ctgccagccc acccagcaaa
      961 cgaaaggtgg ctgccaagat ccagggaaat gccaagaaaa ctctattaaa gtgggtccag
     1021 cacacagcag gcaagcagat gggaatagaa gtgaaagact tcgggaagag ttggagaact
     1081 gggttggcct ttcattcagt catccacgct atccagcctg agctggtgga cctggagaag
     1141 gtgaagacta gatctaacag ggaaaacctg gaggacgcct tcacgatcgc tgaaacacag
     1201 ctaggcatcc ccagactgct tgatcccgaa gatgtggatg tggacaagcc agatgaaaag
     1261 tctatcatga cttacgtggc ccagtttcta acacaatacc ctgacattca tggtgcaggc
     1321 tgtgatgggc aagaggatga tagggatgac agactaattt tgaaggaaac aaaagtttgg
     1381 atagaacaat ttgagagaga ttttacaagg gcacagatga ccgaatcaag tctgcaggac
     1441 aaatatcagg catttaagca cttcagagtt caatatgaaa tgaaaaggaa acaagtggag
     1501 catataatac agccattgca aagagatggc aaactgacgc tcgaccaagc cttggtcaag
     1561 cagtgctggg agagagtatc atcccggctc ttcgattggc atatacagct ggataaatct
     1621 ctccctgcac ctctgggcac tataggtgct tggctataca gggcagaagt ggccctgaga
     1681 gaagagatca ccattcagca ggttcacgag gagacagcaa acacaatcca acggaaactt
     1741 gagcaacata aggatctgct tcaaaataca gatgcccaca aaagggcgtt ccatgaaatc
     1801 taccagacca ggtctgtcaa tgggatcccg atgcctcctg atcagctaga ggacatggct
     1861 gagaggtttc attttgtgtc ctccacatcc gagctgcact taatgaaaat ggagtttctc
     1921 gaactgaagt accgtctgct gtccctgctg gtcctcgctg agtcaaagct gaagtcctgg
     1981 atcatcaagt atgggaggcg agagtctgtg gagctgctgc tgcaaagcta tatctctttt
     2041 atagaaaaca gcaagttctt tgaacagtat gaagtgacat accagatcct gaaacagaca
     2101 gctgacatat acgtcaaagc agagggctca gtggaggaag ctgagaacgt gatgaaattc
     2161 atgagtgaag ccactgccca gtggaggaac ctctccgtgg aagtgcggag tgtacggagc
     2221 atgctggagg aggtgatctc caactgggat cgctatggtg ataccgtggc tagtctccaa
     2281 gcctggctag aagatgcaga gaaaatgcta agccaatcag aacatgccaa gaaggatttt
     2341 tttcgaaatc tgcctcactg gattcagcag cacacagcca tgaacgatgc cgggaatttc
     2401 ctgattgaaa cctgtgatga gattgtttct cgagacctta aacagcagtt gctcttattg
     2461 aacggtcgat ggagggagct cttcatggaa gtcaagcaat atgcaagagc agacgagatg
     2521 gacagaatga agaaagagta tatagacgtc actaccaccc tctttggttt tgcaactgaa
     2581 gcccacagga aactctctga gcctttagaa gtctctttta tcaatgtcaa gttattaatt
     2641 caagacttgg aggatctgga gaagagggtg cctgtgatgg atgctcagta caagatgatt
     2701 gccaagaagg cacatctctt tgccaaagaa agtccccaag aagaagcgaa tgaaatgctt
     2761 acaaccatgt ccaagctcaa agaacagctc tccaaggtca aagaatgttg ctccccactg
     2821 ttgtacgagg cccagcaact gacggttcct ttggaagaac tggagacgca aatcacgtcc
     2881 ttctatgact cccttggaaa aatcaatgag attctatcag ttcttgagca agaagcacaa
     2941 tctagcactc tttttaagca aaaacaccag gaactgttag cttctcaaga gaactgtaag
     3001 aaatccttga cactcataga gaagggcagc caaagtgttc agaagctggt gacctcgagc
     3061 caggcacgaa agccctggga tcacacaaaa ctgcagaagc agatagcaga tgtccatcat
     3121 gcattccaga gcatgatcaa gaaaactgga gactggaaga agcacgtgga agccaacagc
     3181 cgtttgatga agaaatttga ggagtctcgg gccgagttgg agaaggtgct tagggttgct
     3241 caggagggcc tagaggagaa gggggacccc gaggaactcc ttcggagaca cacggagttc
     3301 ttcagccaac tggaccagag ggtcctcaat gctttcctaa aagcctgtga tgagctaact
     3361 gacatcctcc cagagcagga gcaacaaggc ctacaggagg ctgttcgaaa gctacacaaa
     3421 cagtggaagg atcttcaggg agaagcgcct tatcatttgc tccatctgaa aatcgctgtg
     3481 gagaaagaca gattctcagc tgctgtggaa gagtgcagag ctgagctgga gcaagagacc
     3541 aagctggctc ctcaagaagg cagcgagaag atcataaagg aacacagggt tttcttcagt
     3601 gacaagggtc ctcaccatct ctgtgagaag agactacagc tcatagagga attgtgtggg
     3661 aaactccccg tccaggaccc agtaagggac acatgtggag cctgtcacac agctctcaaa
     3721 gagctcaaag cttccattga caacacttac acaatgctcg tggatgaccc agacaagtgg
     3781 aaggactata ccagcaggtt ttctgagttc tcctcttggg tgtctgcaaa gaaggcatgt
     3841 ttgaagaaaa tcaaggatga gcccattgac actggcaacc acgacgaggt taagcatatg
     3901 gtggatgaga tcagaaatga cattaccaag aaaggagaga gtctcagctg gctgaaatcc
     3961 aggcttaagt atttgattga tatttcttca gagaatgaag cccaaaagcg aggagatgag
     4021 ctggcagaat tatcaagctc tttcaaggct cttgtggcat tactgtcaga ggtcgaaaag
     4081 cttctaagca actttggaga atgcgttcag tacaaggaaa tcgtgaaaag ttcccttgag
     4141 gggttgatat cgggccccca agaaagcaag gaagaggctg agatgatcct ggactcgaaa
     4201 aatctgcttg aggcacagca gttgctcctg catcaccagc aaaagacaaa gatgatctca
     4261 gcaaagaaga gagacctcca ggagcagatg gagcaggcac agcagggtgg gcaggcaggc
     4321 ccaggtcaag aggagctgcg gaagctggag agcaccctca caggcctgga gcagagcagg
     4381 gagaggcagg aacgtcgcat ccaggtcagc ttgaggaaat gggagcggtt tgagacaaac
     4441 aaggagacgg tggtcagata tcttttccag acaggttcca gccacgaacg cttcttgagc
     4501 ttcagcagtt tggaaagcct gtcatcagag ttggagcaga caaaggagtt ttctaagagg
     4561 acggaaagca ttgccaccca ggctgagaac ctggtcaagg aagccgcaga gttgccgctg
     4621 gggcccagga ataagcgtgt gcttcagcgg caggccaagt caatcaagga gcaggtcaca
     4681 accctggaag acacgcttga agaagagtat gtgcttcacc acttctaaac gttcctctct
     4741 gagataaaat cccaatagtg tctcctgtgt cttctatatt ccagtctttc caaaatctca
     4801 aggtgtcatc tttatgtttc aagctctctc aaggaagcaa ctcaatttaa aagaaaagta
     4861 tcctcactcc aagaactggt atcaataata acaaaactat acgagggatt tggtgtttgc
     4921 atcttgatat ggaatattca tcactaagcc atatctattc atttctgtgg ccttcacata
     4981 atacagggac aacctaaaaa ctaacccaca actttttttg tttttttttt tttttactat
     5041 tttattatgt tgtcacctgt tagaagtgga ggttttctga gagcatagat tctaggtcta
     5101 agcacacact atgtacaata tatgtctcag agtcaaagct tgtatgtcca tagtgcctct
     5161 gtattcaaac taccatggat taatataacc cttccagaaa ttacctgcaa aactctacct
     5221 tgcttttaaa catgaaatat ttatctgaaa tgaagaaaca atctcttgtg tccctggaaa
     5281 atccttctct agagtttaaa gaacacaagc ttttgatatc attgtcagta tttcttaaaa
     5341 tggtactgct aacgttttta tgtagcaaca cattgaatac tagtctatga ctctcaagaa
     5401 acaggactgt acttgcaaga gggttttggg gaggtgaaaa ctgtttctag tactcttggg
     5461 gccttgcatt caacatttta tatttgccta aattttaaat tttgatcaac attttatttg
     5521 caaccaattc tgactttaaa ctttgacaac attatatttg atatccccca ccaaattctg
     5581 taaacagcaa atctcagaat atattagaaa ataggctgtt tctaaaagga ttagaatatc
     5641 cagttgaagc taaattttat gtaaaaaaaa aaaactgaac tataaaatag attgcatttg
     5701 cctttcttac actgctaagt gcattagaca taaacagggt aataatcttc gactgtgaca
     5761 ctattgaatt tgacaatcaa ttgatgtcat tatttgttct tggcatcgag tgccttttat
     5821 gatcaggtat aacgtccctt catccaagga catcagttaa tttagtgcta aagtgttact
     5881 tttttagttt cttctaaact gaaaaccatg tttactgggt tatgttatgt attcagagct
     5941 agagtcgtat ttgtcagact tactgcacag agatgttgtc tataaataaa gaactcatga
     6001 gcaagagttt aggaaagtct tttggaatca ggtgatctgt ggatagctga gaatgtggta
     6061 ggtcacaaac caaaggcttc gctgagcagg tgagaagagg tgcttccctg tagcctgcga
     6121 ctacaccatg gttctacctt gattcacaag tatgtacttt accaaaggat tttaatataa
     6181 agtgttccaa cggcgcccca ccttgaaagc aaaggcaata gatagaacta tcaaatcccg
     6241 gaagctatgc tgggagagct ccctattctg agttctaaag atggctgctc ctcagcagag
     6301 ctggttcatg ctggttcatg ccctttggag acagcacagt tgaattcatt gtgcacactg
     6361 acactaacag cctagcactt gcactgcctt cccctgagca cattcccaga agagacaggg
     6421 gggctggggt cagatagaca actcttctca agtgtgtgtg ctttcctgct ccaatggttc
     6481 acgcttggaa ggaatcaaat atcctcaaac cctttcacct ggtgatgcac agaggggaaa
     6541 atgtcttgct attcttaatg gagatactgc attttaactc aatttggtta attttagtat
     6601 taaaaccatg gaaatggtga aatccaagtg ggatcatttt ggcagtaatt ttgagaccct
     6661 gtccatctgg atattggaga aagagaatga actcagttcc ttggaagcgt cagcatctgc
     6721 cgcggatgtg cagatcagcc aaatcaaggt gacctgcacc cacgttatgt tactctctct
     6781 tcctttaaga acatctttct tagatttgta tcacccaaag ctctctgatc ccaggacaga
     6841 tcttccgttc ttctgtggct cttttaagtt aaattcatgc caagcttaga gctcatgatt
     6901 gatgacattg tgtgttgtgt atctgactta cagattcaaa gtcaatattg tgtgcaatat
     6961 cattaaacta tttttatggt tg
//
