LOCUS       NM_009296                728 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus SPT4A, DSIF elongation factor subunit (Supt4a),
            transcript variant 1, mRNA.
ACCESSION   NM_009296
VERSION     NM_009296.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 728)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 728)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 728)
  AUTHORS   Cheng,H.M., Chern,Y., Chen,I.H., Liu,C.R., Li,S.H., Chun,S.J.,
            Rigo,F., Bennett,C.F., Deng,N., Feng,Y., Lin,C.S., Yan,Y.T.,
            Cohen,S.N. and Cheng,T.H.
  TITLE     Effects on murine behavior and lifespan of selectively decreasing
            expression of mutant huntingtin allele by supt4h knockdown
  JOURNAL   PLoS Genet 11 (3), e1005043 (2015)
   PUBMED   25760041
  REMARK    GeneRIF: reduction of SUPT4H in mouse brains is associated with
            decreased HTT protein aggregation, and in R6/2 mice, also with
            prolonged lifespan and delay of the motor impairment that normally
            develops in these animals.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 728)
  AUTHORS   Stanlie,A., Begum,N.A., Akiyama,H. and Honjo,T.
  TITLE     The DSIF subunits Spt4 and Spt5 have distinct roles at various
            phases of immunoglobulin class switch recombination
  JOURNAL   PLoS Genet 8 (4), e1002675 (2012)
   PUBMED   22570620
  REMARK    GeneRIF: our findings indicate that Spt4 and Spt5 have essential
            functions in the DNA repair phase of immunoglobulin class switch
            recombination.
REFERENCE   6  (bases 1 to 728)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   7  (bases 1 to 728)
  AUTHORS   Nelson,P.S., Pritchard,C., Abbott,D. and Clegg,N.
  TITLE     The human (PEDB) and mouse (mPEDB) Prostate Expression Databases
  JOURNAL   Nucleic Acids Res 30 (1), 218-220 (2002)
   PUBMED   11752298
REFERENCE   8  (bases 1 to 728)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 728)
  AUTHORS   Chiang,P.W., Zhang,R., Stubbs,L., Zhang,L., Zhu,L. and Kurnit,D.M.
  TITLE     Comparison of murine Supt4h and a nearly identical expressed,
            processed gene: evidence of sequence conservation through gene
            conversion extending into the untranslated regions
  JOURNAL   Nucleic Acids Res 26 (21), 4960-4964 (1998)
   PUBMED   9776760
REFERENCE   10 (bases 1 to 728)
  AUTHORS   Chiang,P.W., Wang,S.Q., Smithivas,P., Song,W.J., Crombez,E.,
            Akhtar,A., Im,R., Greenfield,J., Ramamoorthy,S., Van Keuren,M.,
            Blackburn,C.C., Tsai,C.H. and Kurnit,D.M.
  TITLE     Isolation and characterization of the human and mouse homologues
            (SUPT4H and Supt4h) of the yeast SPT4 gene
  JOURNAL   Genomics 34 (3), 368-375 (1996)
   PUBMED   8786137
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DV061290.1, DV044501.1 and BQ176059.1.
            
            On Sep 27, 2017 this sequence version replaced NM_009296.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BQ176059.1, DV061290.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                DV061290.1         71-104
            35-574              DV044501.1         19-558
            575-728             BQ176059.1         1-154               c
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 52.2 cM"
     gene            1..728
                     /gene="Supt4a"
                     /gene_synonym="Supt4h; Supt4h1; Supt4h1a"
                     /note="SPT4A, DSIF elongation factor subunit"
                     /db_xref="GeneID:20922"
                     /db_xref="MGI:MGI:107416"
     exon            1..118
                     /gene="Supt4a"
                     /gene_synonym="Supt4h; Supt4h1; Supt4h1a"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..403
                     /gene="Supt4a"
                     /gene_synonym="Supt4h; Supt4h1; Supt4h1a"
                     /note="isoform 1 is encoded by transcript variant 1;
                     transcription elongation factor SPT4 1; suppressor of Ty 4
                     homolog 1; DSIF small subunit 1; DRB sensitivity-inducing
                     factor small subunit 1; Transcription elongation factor
                     SPT4-A; suppressor of Ty 4A"
                     /codon_start=1
                     /product="transcription elongation factor SPT4-A isoform
                     1"
                     /protein_id="NP_033322.1"
                     /db_xref="CCDS:CCDS36270.1"
                     /db_xref="GeneID:20922"
                     /db_xref="MGI:MGI:107416"
                     /translation="MALETVPKDLRHLRACLLCSLVKTIDQFEYDGCDNCDAYLQMKG
                     NREMVYDCTSSSFDGIIAMMSPEDSWVSKWQRVSNFKPGVYAVSVTGRLPQGIVRELK
                     SRGVAYKSRDTAIKT"
     exon            119..225
                     /gene="Supt4a"
                     /gene_synonym="Supt4h; Supt4h1; Supt4h1a"
                     /inference="alignment:Splign:2.1.0"
     exon            226..281
                     /gene="Supt4a"
                     /gene_synonym="Supt4h; Supt4h1; Supt4h1a"
                     /inference="alignment:Splign:2.1.0"
     exon            282..335
                     /gene="Supt4a"
                     /gene_synonym="Supt4h; Supt4h1; Supt4h1a"
                     /inference="alignment:Splign:2.1.0"
     exon            336..713
                     /gene="Supt4a"
                     /gene_synonym="Supt4h; Supt4h1; Supt4h1a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctgtttactt cctgcggctg gaggaacctg tgttgtcgta tcggtgagga tggccctgga
       61 gacggtacca aaggacctgc ggcatctgcg ggcttgtttg ctgtgctcgt tagtcaagac
      121 tatagaccag ttcgaatatg atgggtgtga caattgcgat gcatacctac aaatgaaggg
      181 caacagagag atggtttatg actgcaccag ctcttcattt gatggaatca ttgcgatgat
      241 gagtccagag gacagctggg tctccaagtg gcagcgagtc agtaacttta agccaggtgt
      301 atatgctgtg tccgtcactg gtcgcctgcc ccaaggaatc gtgcgggagc tgaaaagtcg
      361 aggagtggcc tacaaatcca gagacacagc aataaagacc tagctaggtg caggccatag
      421 catcttgttc ttcacttctg tacttgtttt cttgtggaac taaaccgtgg gaccccaaag
      481 actccccgcc ctccatctcc gaatcagcag actccacgaa agcagcacat cggccttaag
      541 tcatctcact ttctctgccc actggtgaag tgggagtgtc tgggcggatt ctcagaggct
      601 gtgtcaacag aggaagatgc tgatttccca cctgtgttca gggttttgcc tttgtctatg
      661 ggcaggactt tggttcaaat accaataaag cgagcctttg gaacatactt aaaaaaaaaa
      721 aaaaaaaa
//
