LOCUS       NP_291096               1047 aa            linear   ROD 27-APR-2025
DEFINITION  FACT complex subunit SPT16 [Mus musculus].
ACCESSION   NP_291096
VERSION     NP_291096.2
DBSOURCE    REFSEQ: accession NM_033618.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 1047)
  AUTHORS   Wang,J., Zhu,X., Dai,L., Wang,Z., Guan,X., Tan,X., Li,J., Zhang,M.,
            Bai,Y. and Guo,H.
  TITLE     Supt16 haploinsufficiency causes neurodevelopment disorder by
            disrupting MAPK pathway in neural stem cells
  JOURNAL   Hum Mol Genet 32 (5), 860-872 (2023)
   PUBMED   36226587
  REMARK    GeneRIF: Supt16 haploinsufficiency causes neurodevelopment disorder
            by disrupting MAPK pathway in neural stem cells.
REFERENCE   2  (residues 1 to 1047)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 1047)
  AUTHORS   Chen,F., Zhang,W., Xie,D., Gao,T., Dong,Z. and Lu,X.
  TITLE     Histone chaperone FACT represses retrotransposon MERVL and
            MERVL-derived cryptic promoters
  JOURNAL   Nucleic Acids Res 48 (18), 10211-10225 (2020)
   PUBMED   32894293
  REMARK    GeneRIF: Histone chaperone FACT represses retrotransposon MERVL and
            MERVL-derived cryptic promoters.
REFERENCE   4  (residues 1 to 1047)
  AUTHORS   Liu,X., Jiao,Y., Cao,Y., Deng,N., Ma,Y., Hasty,K.A., Kang,A.,
            Chen,H., Stuart,J.M. and Gu,W.
  TITLE     Decreased expression levels of Ifi genes is associated to the
            increased resistance to spontaneous arthritis disease in mice
            deficiency of IL-1RA
  JOURNAL   BMC Immunol 17 (1), 25 (2016)
   PUBMED   27480124
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 1047)
  AUTHORS   Husain,A., Begum,N.A., Taniguchi,T., Taniguchi,H., Kobayashi,M. and
            Honjo,T.
  TITLE     Chromatin remodeller SMARCA4 recruits topoisomerase 1 and
            suppresses transcription-associated genomic instability
  JOURNAL   Nat Commun 7, 10549 (2016)
   PUBMED   26842758
  REMARK    GeneRIF: FACT is required for TOP1 binding to H3K4me3 at non-B DNA
            containing chromatin for the site-specific cleavage.
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 1047)
  AUTHORS   Tan,J.J., Ong,S.A. and Chen,K.S.
  TITLE     Rasd1 interacts with Ear2 (Nr2f6) to regulate renin transcription
  JOURNAL   BMC Mol Biol 12, 4 (2011)
   PUBMED   21247419
  REMARK    Publication Status: Online-Only
REFERENCE   7  (residues 1 to 1047)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   8  (residues 1 to 1047)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   9  (residues 1 to 1047)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (residues 1 to 1047)
  AUTHORS   McGuire,M.V., Suthipinijtham,P. and Gascoigne,N.R.
  TITLE     The mouse Supt16h/Fact140 gene, encoding part of the FACT chromatin
            transcription complex, maps close to Tcra and is highly expressed
            in thymus
  JOURNAL   Mamm Genome 12 (8), 664-667 (2001)
   PUBMED   11471063
  REMARK    GeneRIF: The mouse Supt16h/Fact140 gene, encoding part of the FACT
            chromatin transcription complex, maps close to Tcra and is highly
            expressed in thymus
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AI930189.1, AK148506.1, AF323667.1, AC159323.3 and CB202003.1.
            
            On Aug 1, 2007 this sequence version replaced NP_291096.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF323667.1, SRR1660815.117488.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164137, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 26.83 cM"
     Protein         1..1047
                     /product="FACT complex subunit SPT16"
                     /note="FACTp140; FACT 140 kDa subunit; facilitates
                     chromatin transcription complex subunit SPT16;
                     chromatin-specific transcription elongation factor 140 kDa
                     subunit; suppressor of Ty 16 homolog"
                     /calculated_mol_wt=119708
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylalanine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Region          5..1001
                     /region_name="COG5406"
                     /note="Nucleosome binding factor SPN, SPT16 subunit
                     [Transcription, Replication, recombination and repair,
                     Chromatin structure and dynamics]"
                     /db_xref="CDD:227693"
     Site            139
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            188
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            196
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0007744|PubMed:23806337; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            223
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            455
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Region          491..518
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            508
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            513
                     /site_type="acetylation"
                     /note="N6-acetyllysine, alternate.
                     /evidence=ECO:0007744|PubMed:23806337; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            650
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            658
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            732
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            786
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            903
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            904
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Region          918..1047
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            979
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            982
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            986
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     Site            1015
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5B9; propagated from
                     UniProtKB/Swiss-Prot (Q920B9.2)"
     CDS             1..1047
                     /gene="Supt16"
                     /gene_synonym="Cdc68; Fact140; Spt16; Supt16h"
                     /coded_by="NM_033618.3:90..3233"
                     /db_xref="CCDS:CCDS27051.1"
                     /db_xref="GeneID:114741"
                     /db_xref="MGI:MGI:1890948"
ORIGIN      
        1 mavtldkday yrrvkrlysn wrkgedeyas idaivvsvgv deeivyakst alqtwlfgye
       61 ltdtimvfcd dkiifmaskk kveflkqian tkgnenanga paitllvrek nesnkssfdk
      121 midaikesks gkkigvfskd kfpgefmksw sdclnkegfd kvdisavvay tiavkedgel
      181 nlmkkaasit sevfnkffke rvmeivdade kvrhsklaes vekaieekky lagadpstve
      241 mcyppiiqsg gnynlkfsvv sdknhmhfga itcamgirfk sycsnlvrtl mvdptqevqe
      301 nynfllqlqe ellkelrhgv kicdvynsvm dvvkkqkpel lnkitknlgf gmgiefregs
      361 lvinsknqyk lkkgmvfsin lgfsdltnke gkkpeektya lfigdtvlvd edgpatilts
      421 vkkkvknvgi flkneddeee eeekdeaedl lgrgsraall tertrnemta eekrrahqke
      481 laaqlneeak rrlteqkgeq qiqkarksnv syknpslmpk ephiremkiy idkkyetvim
      541 pvfgiatpfh iatiknisms vegdytylri nfycpgsalg rnegnifpnp eatfvkeity
      601 rasnmkapge qtvpalnlqn afriikevqk ryktreaeek ekegivkqds lvinlnrsnp
      661 klkdlyirpn iaqkrmqgsl eahvngfrft svrgdkvdil ynnikhalfq pcdgemiivl
      721 hfhlknaimf gkkrhtdvqf ytevgeittd lgkhqhmhdr ddlyaeqmer emrhklktaf
      781 knfiekveal tkeelefevp frdlgfngap yrstcllqpt ssalvnatew ppfvvtldev
      841 elihfervqf hlknfdmviv ykdyskkvtm inaipvasld pikewlnscd lkytegvqsl
      901 nwtkimktiv ddpegffeqg gwsflepege gsdaedgdse seiedetfnp seddyeeeee
      961 dsdedyssea eesdyskesl gseeesgkdw deleeearka dresryeeee eqsrsmsrkr
     1021 kasvhssgrg snrgsrhssa ppkkkrk
//
