LOCUS       NM_029599               1172 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus Sad1 and UNC84 domain containing 5 (Sun5), transcript
            variant 2, mRNA.
ACCESSION   NM_029599 XM_905722
VERSION     NM_029599.3
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Zhang,Y., Liu,C., Wu,B., Li,L., Li,W. and Yuan,L.
  TITLE     The missing linker between SUN5 and PMFBP1 in sperm head-tail
            coupling apparatus
  JOURNAL   Nat Commun 12 (1), 4926 (2021)
   PUBMED   34389728
  REMARK    GeneRIF: The missing linker between SUN5 and PMFBP1 in sperm
            head-tail coupling apparatus.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1172)
  AUTHORS   Zhang,Y., Yang,L., Huang,L., Liu,G., Nie,X., Zhang,X. and Xing,X.
  TITLE     SUN5 Interacting With Nesprin3 Plays an Essential Role in Sperm
            Head-to-Tail Linkage: Research on Sun5 Gene Knockout Mice
  JOURNAL   Front Cell Dev Biol 9, 684826 (2021)
   PUBMED   34268309
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1172)
  AUTHORS   Shang,Y., Yan,J., Tang,W., Liu,C., Xiao,S., Guo,Y., Yuan,L.,
            Chen,L., Jiang,H., Guo,X., Qiao,J. and Li,W.
  TITLE     Mechanistic insights into acephalic spermatozoa syndrome-associated
            mutations in the human SUN5 gene
  JOURNAL   J Biol Chem 293 (7), 2395-2407 (2018)
   PUBMED   29298896
REFERENCE   5  (bases 1 to 1172)
  AUTHORS   Shang,Y., Zhu,F., Wang,L., Ouyang,Y.C., Dong,M.Z., Liu,C., Zhao,H.,
            Cui,X., Ma,D., Zhang,Z., Yang,X., Guo,Y., Liu,F., Yuan,L., Gao,F.,
            Guo,X., Sun,Q.Y., Cao,Y. and Li,W.
  TITLE     Essential role for SUN5 in anchoring sperm head to the tail
  JOURNAL   Elife 6, e28199 (2017)
   PUBMED   28945193
  REMARK    GeneRIF: These studies reveal the essential role of SUN5 in
            anchoring sperm head to the tail and provide a promising way to
            treat this kind of acephalic spermatozoa-associated male
            infertility.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1172)
  AUTHORS   Jiang,X.Z., Yang,M.G., Huang,L.H., Li,C.Q. and Xing,X.W.
  TITLE     SPAG4L, a novel nuclear envelope protein involved in the meiotic
            stage of spermatogenesis
  JOURNAL   DNA Cell Biol 30 (11), 875-882 (2011)
   PUBMED   21711156
  REMARK    GeneRIF: SPAG4L may play an important role in the meiotic stage of
            spermatogenesis
REFERENCE   7  (bases 1 to 1172)
  AUTHORS   Frohnert,C., Schweizer,S. and Hoyer-Fender,S.
  TITLE     SPAG4L/SPAG4L-2 are testis-specific SUN domain proteins restricted
            to the apical nuclear envelope of round spermatids facing the
            acrosome
  JOURNAL   Mol Hum Reprod 17 (4), 207-218 (2011)
   PUBMED   21159740
  REMARK    GeneRIF: SPAG4L-2 and SPAG4L are transmembrane proteins that
            localize to the nuclear envelope.
REFERENCE   8  (bases 1 to 1172)
  AUTHORS   Xing,X.W., Yuan,H. and Wang,W.
  TITLE     [Prokaryotic expression and purification of SRG4, a novel mouse
            spermatogenesis gene]
  JOURNAL   Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 23 (8), 701-703 (2007)
   PUBMED   17618558
  REMARK    GeneRIF: SRG4 was cloned into pUCm-T vectors and sequenced,
            subcloned into a prokaryotic expression vector and expression of
            induced by IPTG.
REFERENCE   9  (bases 1 to 1172)
  AUTHORS   Xing,X.W., Li,L.Y. and Lu,G.X.
  TITLE     [Expression of a novel spermatogenesis gene SRG4 in postpartum
            normal and cryptorchid mouse testis]
  JOURNAL   Yi Chuan 29 (6), 699-704 (2007)
   PUBMED   17650487
  REMARK    GeneRIF: Results indicated that the expression of SRG4 was
            regulated by development, and SRG4 was mainly expressed in the
            cytoplasm of spermatocytes and round spermatids.
REFERENCE   10 (bases 1 to 1172)
  AUTHORS   Xing,X.W., Li,L.Y., Liu,G. and Lu,G.X.
  TITLE     [Cloning of cDNA of SRG4, a mouse spermatogenesis related gene and
            expression in mouse different developing stages]
  JOURNAL   Yi Chuan Xue Bao 31 (10), 1066-1071 (2004)
   PUBMED   15552040
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL833803.8.
            
            On Dec 22, 2022 this sequence version replaced NM_029599.2.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 5' coding region compared to variant 1. It encodes
            isoform 2 which is shorter compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY307077.1, AK006225.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-175               AL833803.8         162594-162768       c
            176-231             AL833803.8         161084-161139       c
            232-298             AL833803.8         159888-159954       c
            299-360             AL833803.8         159179-159240       c
            361-410             AL833803.8         158571-158620       c
            411-445             AL833803.8         157639-157673       c
            446-554             AL833803.8         156973-157081       c
            555-633             AL833803.8         153098-153176       c
            634-749             AL833803.8         152256-152371       c
            750-917             AL833803.8         150626-150793       c
            918-1004            AL833803.8         150092-150178       c
            1005-1172           AL833803.8         147857-148024       c
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 75.99 cM"
     gene            1..1172
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /note="Sad1 and UNC84 domain containing 5"
                     /db_xref="GeneID:76407"
                     /db_xref="MGI:MGI:1923657"
     exon            1..175
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..1148
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /note="isoform 2 is encoded by transcript variant 2;
                     sperm-associated antigen 4-like protein; SUN
                     domain-containing protein 5; spermatogenesis-related gene
                     4"
                     /codon_start=1
                     /product="SUN domain-containing protein 5 isoform 2"
                     /protein_id="NP_083875.1"
                     /db_xref="CCDS:CCDS38288.1"
                     /db_xref="GeneID:76407"
                     /db_xref="MGI:MGI:1923657"
                     /translation="MPRTRNIGALCTLPEDTTHSGRPRRGVQRSYISRMAEPAPANMT
                     WLTYLACFLRTQTQQVFLNTCRCKLFCQKVMEKMGLLVLCVFGFWMFSMHLPSKVEVW
                     QDDSINGPLQSLRMYQEKVRHHTGEIQDLRGSMNQLIAKLQKMEAISDEQKMAQKIMK
                     MIQGDYIEKPDFALKSIGASIDFEHTSATYNHDKARSYWNWIRLWNYAQPPDVILEPN
                     VTPGNCWAFASDRGQVTIRLAQKVYLSNITLQHIPKTISLSGSLDTAPKDFVIYGLES
                     LPREEVFLGAFQFQPENIIQTFQLQNLPPRSFAAVKVKISSNWGNPRFTCMYRVRVHG
                     SVTPPKDSHLEPLS"
     exon            176..231
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            232..298
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            299..360
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            361..410
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            411..445
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            446..554
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            555..633
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            634..749
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            750..917
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1004
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1172
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1149..1154
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /note="hexamer: AGTAAA"
     polyA_site      1172
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /note="major polyA site"
ORIGIN      
        1 ccttccaggg aatactcaat ggtggtggcg gcagtggcag ccacttcctc ctcattgact
       61 cccatagacc tccatccttg gccctttata aaaggaaatc aatgccccgg acgaggaaca
      121 tcggggcctt gtgtacccta cctgaagaca caactcacag tggcaggcct aggaggggcg
      181 tccaaagaag ctatatcagc agaatggcag agccagcccc ggcaaacatg acctggctca
      241 cctacctagc ctgcttcctg aggactcaga cccagcaagt tttccttaac acctgcagat
      301 gcaagttgtt ctgccagaaa gtcatggaaa agatgggcct tctggtcctc tgtgtttttg
      361 gcttctggat gttttccatg cacttaccat caaaagtgga agtctggcag gatgacagca
      421 taaatggtcc attgcagagc ctgaggatgt accaggagaa ggtacgacat cacactgggg
      481 aaatccagga cctacgaggt agcatgaacc agctcattgc caaactccag aagatggaag
      541 ccatatctga tgagcaaaaa atggcccaga aaataatgaa gatgattcaa ggtgattaca
      601 ttgaaaagcc agactttgcc ctgaagtcca taggggccag catcgacttc gagcacacgt
      661 cagccacgta taaccacgac aaggctcgct cctactggaa ctggatccga ctgtggaact
      721 acgcgcagcc cccggatgtg atccttgagc caaacgtgac acctgggaac tgctgggcct
      781 ttgccagtga ccgcggccag gtgaccatcc gtttggccca gaaagtctac ctgtccaaca
      841 ttacactgca gcacattcct aagacaatct cgttgtcggg aagcctggac accgcaccaa
      901 aggactttgt catctatggc ttggagagcc tacccaggga ggaagtgttc ctaggggcat
      961 tccagtttca gccagaaaac atcatccaaa cgtttcagct ccagaacctg ccacccagga
     1021 gctttgctgc agtcaaggtg aagatctcca gcaactgggg gaaccctcgc ttcacctgca
     1081 tgtaccgtgt gcgtgtgcac ggctctgtaa cccctcccaa agactcacac ctagagcccc
     1141 tatcctagag taaagtatat tattcagtcc aa
//
