LOCUS       NM_001305048            1247 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus Sad1 and UNC84 domain containing 5 (Sun5), transcript
            variant 1, mRNA.
ACCESSION   NM_001305048 XM_006500373
VERSION     NM_001305048.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Zhang,Y., Liu,C., Wu,B., Li,L., Li,W. and Yuan,L.
  TITLE     The missing linker between SUN5 and PMFBP1 in sperm head-tail
            coupling apparatus
  JOURNAL   Nat Commun 12 (1), 4926 (2021)
   PUBMED   34389728
  REMARK    GeneRIF: The missing linker between SUN5 and PMFBP1 in sperm
            head-tail coupling apparatus.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1247)
  AUTHORS   Zhang,Y., Yang,L., Huang,L., Liu,G., Nie,X., Zhang,X. and Xing,X.
  TITLE     SUN5 Interacting With Nesprin3 Plays an Essential Role in Sperm
            Head-to-Tail Linkage: Research on Sun5 Gene Knockout Mice
  JOURNAL   Front Cell Dev Biol 9, 684826 (2021)
   PUBMED   34268309
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1247)
  AUTHORS   Shang,Y., Yan,J., Tang,W., Liu,C., Xiao,S., Guo,Y., Yuan,L.,
            Chen,L., Jiang,H., Guo,X., Qiao,J. and Li,W.
  TITLE     Mechanistic insights into acephalic spermatozoa syndrome-associated
            mutations in the human SUN5 gene
  JOURNAL   J Biol Chem 293 (7), 2395-2407 (2018)
   PUBMED   29298896
REFERENCE   5  (bases 1 to 1247)
  AUTHORS   Shang,Y., Zhu,F., Wang,L., Ouyang,Y.C., Dong,M.Z., Liu,C., Zhao,H.,
            Cui,X., Ma,D., Zhang,Z., Yang,X., Guo,Y., Liu,F., Yuan,L., Gao,F.,
            Guo,X., Sun,Q.Y., Cao,Y. and Li,W.
  TITLE     Essential role for SUN5 in anchoring sperm head to the tail
  JOURNAL   Elife 6, e28199 (2017)
   PUBMED   28945193
  REMARK    GeneRIF: These studies reveal the essential role of SUN5 in
            anchoring sperm head to the tail and provide a promising way to
            treat this kind of acephalic spermatozoa-associated male
            infertility.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1247)
  AUTHORS   Jiang,X.Z., Yang,M.G., Huang,L.H., Li,C.Q. and Xing,X.W.
  TITLE     SPAG4L, a novel nuclear envelope protein involved in the meiotic
            stage of spermatogenesis
  JOURNAL   DNA Cell Biol 30 (11), 875-882 (2011)
   PUBMED   21711156
  REMARK    GeneRIF: SPAG4L may play an important role in the meiotic stage of
            spermatogenesis
REFERENCE   7  (bases 1 to 1247)
  AUTHORS   Frohnert,C., Schweizer,S. and Hoyer-Fender,S.
  TITLE     SPAG4L/SPAG4L-2 are testis-specific SUN domain proteins restricted
            to the apical nuclear envelope of round spermatids facing the
            acrosome
  JOURNAL   Mol Hum Reprod 17 (4), 207-218 (2011)
   PUBMED   21159740
  REMARK    GeneRIF: SPAG4L-2 and SPAG4L are transmembrane proteins that
            localize to the nuclear envelope.
REFERENCE   8  (bases 1 to 1247)
  AUTHORS   Xing,X.W., Yuan,H. and Wang,W.
  TITLE     [Prokaryotic expression and purification of SRG4, a novel mouse
            spermatogenesis gene]
  JOURNAL   Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 23 (8), 701-703 (2007)
   PUBMED   17618558
  REMARK    GeneRIF: SRG4 was cloned into pUCm-T vectors and sequenced,
            subcloned into a prokaryotic expression vector and expression of
            induced by IPTG.
REFERENCE   9  (bases 1 to 1247)
  AUTHORS   Xing,X.W., Li,L.Y. and Lu,G.X.
  TITLE     [Expression of a novel spermatogenesis gene SRG4 in postpartum
            normal and cryptorchid mouse testis]
  JOURNAL   Yi Chuan 29 (6), 699-704 (2007)
   PUBMED   17650487
  REMARK    GeneRIF: Results indicated that the expression of SRG4 was
            regulated by development, and SRG4 was mainly expressed in the
            cytoplasm of spermatocytes and round spermatids.
REFERENCE   10 (bases 1 to 1247)
  AUTHORS   Xing,X.W., Li,L.Y., Liu,G. and Lu,G.X.
  TITLE     [Cloning of cDNA of SRG4, a mouse spermatogenesis related gene and
            expression in mouse different developing stages]
  JOURNAL   Yi Chuan Xue Bao 31 (10), 1066-1071 (2004)
   PUBMED   15552040
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL833803.8.
            
            On Dec 22, 2022 this sequence version replaced NM_001305048.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FJ667498.1, SRR5189685.255804.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-175               AL833803.8         162594-162768       c
            176-231             AL833803.8         161084-161139       c
            232-306             AL833803.8         160853-160927       c
            307-373             AL833803.8         159888-159954       c
            374-435             AL833803.8         159179-159240       c
            436-485             AL833803.8         158571-158620       c
            486-520             AL833803.8         157639-157673       c
            521-629             AL833803.8         156973-157081       c
            630-708             AL833803.8         153098-153176       c
            709-824             AL833803.8         152256-152371       c
            825-992             AL833803.8         150626-150793       c
            993-1079            AL833803.8         150092-150178       c
            1080-1247           AL833803.8         147857-148024       c
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 75.99 cM"
     gene            1..1247
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /note="Sad1 and UNC84 domain containing 5"
                     /db_xref="GeneID:76407"
                     /db_xref="MGI:MGI:1923657"
     exon            1..175
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..1223
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sperm-associated antigen 4-like protein; SUN
                     domain-containing protein 5; spermatogenesis-related gene
                     4"
                     /codon_start=1
                     /product="SUN domain-containing protein 5 isoform 1"
                     /protein_id="NP_001291977.1"
                     /db_xref="GeneID:76407"
                     /db_xref="MGI:MGI:1923657"
                     /translation="MPRTRNIGALCTLPEDTTHSGRPRRGVQRSYISRMAEPAPANMN
                     DPLLLPLRMNTPGLSLVQILLGYMSWLTYLACFLRTQTQQVFLNTCRCKLFCQKVMEK
                     MGLLVLCVFGFWMFSMHLPSKVEVWQDDSINGPLQSLRMYQEKVRHHTGEIQDLRGSM
                     NQLIAKLQKMEAISDEQKMAQKIMKMIQGDYIEKPDFALKSIGASIDFEHTSATYNHD
                     KARSYWNWIRLWNYAQPPDVILEPNVTPGNCWAFASDRGQVTIRLAQKVYLSNITLQH
                     IPKTISLSGSLDTAPKDFVIYGLESLPREEVFLGAFQFQPENIIQTFQLQNLPPRSFA
                     AVKVKISSNWGNPRFTCMYRVRVHGSVTPPKDSHLEPLS"
     exon            176..231
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            232..306
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            307..373
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            374..435
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            436..485
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            486..520
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            521..629
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            630..708
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            709..824
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            825..992
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1079
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1247
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1224..1229
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /note="hexamer: AGTAAA"
     polyA_site      1247
                     /gene="Sun5"
                     /gene_synonym="1700021O15Rik; Spag4l; SPAG4L-2; SRG4"
                     /note="major polyA site"
ORIGIN      
        1 ccttccaggg aatactcaat ggtggtggcg gcagtggcag ccacttcctc ctcattgact
       61 cccatagacc tccatccttg gccctttata aaaggaaatc aatgccccgg acgaggaaca
      121 tcggggcctt gtgtacccta cctgaagaca caactcacag tggcaggcct aggaggggcg
      181 tccaaagaag ctatatcagc agaatggcag agccagcccc ggcaaacatg aatgaccccc
      241 ttctgttgcc tctccgcatg aacaccccag gcctcagcct agtccagatc ctactgggat
      301 atatgtcctg gctcacctac ctagcctgct tcctgaggac tcagacccag caagttttcc
      361 ttaacacctg cagatgcaag ttgttctgcc agaaagtcat ggaaaagatg ggccttctgg
      421 tcctctgtgt ttttggcttc tggatgtttt ccatgcactt accatcaaaa gtggaagtct
      481 ggcaggatga cagcataaat ggtccattgc agagcctgag gatgtaccag gagaaggtac
      541 gacatcacac tggggaaatc caggacctac gaggtagcat gaaccagctc attgccaaac
      601 tccagaagat ggaagccata tctgatgagc aaaaaatggc ccagaaaata atgaagatga
      661 ttcaaggtga ttacattgaa aagccagact ttgccctgaa gtccataggg gccagcatcg
      721 acttcgagca cacgtcagcc acgtataacc acgacaaggc tcgctcctac tggaactgga
      781 tccgactgtg gaactacgcg cagcccccgg atgtgatcct tgagccaaac gtgacacctg
      841 ggaactgctg ggcctttgcc agtgaccgcg gccaggtgac catccgtttg gcccagaaag
      901 tctacctgtc caacattaca ctgcagcaca ttcctaagac aatctcgttg tcgggaagcc
      961 tggacaccgc accaaaggac tttgtcatct atggcttgga gagcctaccc agggaggaag
     1021 tgttcctagg ggcattccag tttcagccag aaaacatcat ccaaacgttt cagctccaga
     1081 acctgccacc caggagcttt gctgcagtca aggtgaagat ctccagcaac tgggggaacc
     1141 ctcgcttcac ctgcatgtac cgtgtgcgtg tgcacggctc tgtaacccct cccaaagact
     1201 cacacctaga gcccctatcc tagagtaaag tatattattc agtccaa
//
