LOCUS       NM_172294               5469 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus sulfatase 1 (Sulf1), transcript variant 2, mRNA.
ACCESSION   NM_172294
VERSION     NM_172294.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Kay,R.G.G., Reeves,R., Siggers,P., Greenaway,S., Mallon,A.M.,
            Wells,S., Koo,B.K., Mayere,C., Nef,S., Greenfield,A. and Simon,M.M.
  TITLE     Gonadal sex reversal at single-cell resolution in Znrf3-deficient
            mice
  JOURNAL   Development 151 (23) (2024)
   PUBMED   39629665
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Whitehead,A.K., Wang,Z., Boustany,R.J., Vives,R.R., Lazartigues,E.,
            Liu,J., Siggins,R.W. and Yue,X.
  TITLE     Myeloid deficiency of heparan sulfate 6-O-endosulfatases impairs
            bone marrow hematopoiesis
  JOURNAL   Matrix Biol 134, 107-118 (2024)
   PUBMED   39368561
REFERENCE   3  (bases 1 to 5469)
  AUTHORS   Xiao,Z., Liu,S., Li,Z., Cui,J., Wang,H., Wang,Z., Ren,Q., Xia,L.,
            Wang,Z. and Li,Y.
  TITLE     The Maternal Microbiome Programs the m6A Epitranscriptome of the
            Mouse Fetal Brain and Intestine
  JOURNAL   Front Cell Dev Biol 10, 882994 (2022)
   PUBMED   35874829
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5469)
  AUTHORS   Tsuji,Y., Kerever,A., Furukawa,T., Kamagata,K., Saito,Y., Aoki,S.,
            Hata,J., Okano,H., Kobayashi,K., Okada,T., Miya,K., Keino-Masu,K.,
            Masu,M. and Arikawa-Hirasawa,E.
  TITLE     Diffusion magnetic resonance tractography-based evaluation of
            commissural fiber abnormalities in a heparan sulfate
            endosulfatase-deficient mouse brain
  JOURNAL   Magn Reson Imaging 88, 123-131 (2022)
   PUBMED   35131262
REFERENCE   5  (bases 1 to 5469)
  AUTHORS   Miya,K., Keino-Masu,K., Okada,T., Kobayashi,K. and Masu,M.
  TITLE     Expression of Heparan Sulfate Endosulfatases in the Adult Mouse
            Brain: Co-expression of Sulf1 and Dopamine D1/D2 Receptors
  JOURNAL   Front Neuroanat 15, 726718 (2021)
   PUBMED   34489650
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5469)
  AUTHORS   Lamanna,W.C., Baldwin,R.J., Padva,M., Kalus,I., Ten Dam,G., van
            Kuppevelt,T.H., Gallagher,J.T., von Figura,K., Dierks,T. and
            Merry,C.L.
  TITLE     Heparan sulfate 6-O-endosulfatases: discrete in vivo activities and
            functional co-operativity
  JOURNAL   Biochem J 400 (1), 63-73 (2006)
   PUBMED   16901266
REFERENCE   7  (bases 1 to 5469)
  AUTHORS   Mingin,G., Stahl,D.A., Chacko,J. and Koul,H.
  TITLE     Global gene expression patterns in mouse wolffian duct development
  JOURNAL   J Urol 176 (4 Pt 2), 1701-1705 (2006)
   PUBMED   16945627
REFERENCE   8  (bases 1 to 5469)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   9  (bases 1 to 5469)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 5469)
  AUTHORS   Morimoto-Tomita,M., Uchimura,K., Werb,Z., Hemmerich,S. and
            Rosen,S.D.
  TITLE     Cloning and characterization of two extracellular heparin-degrading
            endosulfatases in mice and humans
  JOURNAL   J Biol Chem 277 (51), 49175-49185 (2002)
   PUBMED   12368295
  REMARK    GeneRIF: Sulfs are extracellular endosulfatases with strong
            potential for modulating the interactions of heparan sulfate
            proteoglycans in the extracellular microenvironment
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC136316.13, AC134787.6 and AC126044.4.
            
            On Feb 7, 2022 this sequence version replaced NM_172294.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. All three variants encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC049276.1, AY101178.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-280               AC136316.13        180817-181096
            281-375             AC136316.13        190907-191001
            376-448             AC134787.6         36338-36410         c
            449-679             AC134787.6         35791-36021         c
            680-919             AC134787.6         25484-25723         c
            920-1071            AC134787.6         17158-17309         c
            1072-1241           AC134787.6         14466-14635         c
            1242-1392           AC134787.6         5001-5151           c
            1393-1568           AC134787.6         3936-4111           c
            1569-1697           AC134787.6         1960-2088           c
            1698-1754           AC134787.6         1638-1694           c
            1755-1884           AC134787.6         390-519             c
            1885-2101           AC126044.4         100632-100848
            2102-2354           AC126044.4         102878-103130
            2355-2451           AC126044.4         104348-104444
            2452-2546           AC126044.4         104922-105016
            2547-2612           AC126044.4         105962-106027
            2613-2788           AC126044.4         107168-107343
            2789-2931           AC126044.4         112614-112756
            2932-3055           AC126044.4         112850-112973
            3056-3089           AC126044.4         115279-115312
            3090-5469           AC126044.4         123331-125710
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 3.35 cM"
     gene            1..5469
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /note="sulfatase 1"
                     /db_xref="GeneID:240725"
                     /db_xref="MGI:MGI:2138563"
     exon            1..280
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            281..375
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            376..448
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            449..679
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             508..3120
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /EC_number="3.1.6.1"
                     /EC_number="3.1.6.14"
                     /note="extracellular sulfatase SULF-1; arylsulfatase;
                     N-acetylglucosamine-6-sulfatase"
                     /codon_start=1
                     /product="extracellular sulfatase Sulf-1 precursor"
                     /protein_id="NP_758498.1"
                     /db_xref="CCDS:CCDS14820.1"
                     /db_xref="GeneID:240725"
                     /db_xref="MGI:MGI:2138563"
                     /translation="MKYSLWALLLAVLGTQLLGSLCSTVRSQRFRGRIQQERKNIRPN
                     IILVLTDDQDVELGSLQVMNKTRKIMEQGGATFTNAFVTTPMCCPSRSSMLTGKYVHN
                     HNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWL
                     GLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESINYFKMSKRMYPHRPIMMV
                     ISHAAPHGPEDSAPQFSKLYPNASQHITPSYNYAPNMDKHWIMQYTGPMLPIHMEFTN
                     VLQRKRLQTLMSVDDSVERLYNMLVESGELDNTYIIYTADHGYHIGQFGLVKGKSMPY
                     DFDIRVPFFIRGPSIEPGSIVPQIVLNIDLAPTILDIAGLDSPSDVDGKSVLKLLDLE
                     KPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESGKNIQQSNHLPKYERVKELCQQARY
                     QTACEQPGQNWQCIEDTSGKLRIHKCKGPSDLLTVRQNARNLYSRGLHDKDKECHCRD
                     SGYRSSRSQRKNQRQFLRNKGTPKYKPRFVHTRQTRSLSVEFEGEIYDINLEEEELQV
                     LPPRSIAKRHDEGHQGFIGHQAAAGDIRNEMLADSNNAVGLPATVRVTHKCFILPNDT
                     IHCERELYQSARAWKDHKAYIDKEIEVLQDKIKNLREVRGHLKKRKPEECGCGDQSYY
                     NKEKGVKRQEKLKSHLHPFKEAAAQEVDSKLQLFKEHRRRKKERKEKKRQRKGEECSL
                     PGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETHNFLFCEFATGFLE
                     YFDMNTDPYQLTNTVHTVERSILNQLHIQLMELRSCQGYKQCNPRPKSLDIGAKEGGN
                     YDPHRGQLWDGWEG"
     exon            680..919
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1071
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1241
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1392
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1568
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1697
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..1754
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1884
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..2101
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2102..2354
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2355..2451
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2452..2546
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2547..2612
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2613..2788
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2789..2931
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2932..3055
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3056..3089
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3090..5469
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4635..4640
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /note="hexamer: AATAAA"
     polyA_site      4655
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
     regulatory      5449..5454
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /note="hexamer: AATAAA"
     polyA_site      5469
                     /gene="Sulf1"
                     /gene_synonym="mKIAA1077; MSulf-1"
                     /note="major polyA site"
ORIGIN      
        1 agtcagtccc taatgaatct tggaactggt gtcaggtcgg gcagttttat ctgcaagttg
       61 gaaatccgag gcccagaggc caggagagag ggcggctggg atcagaggcc aagcctccct
      121 ccctggctcc tggagccccg cagaggtcag ggcgataagg aacaatgact ctcccattca
      181 ggaggaggaa ggaagtccct gctgccacct aatctctgct cctctgcttc tcccctgctc
      241 ccgagccttt tctctagaga agactttcaa ggcgactttt gtgctgaggg ccagccgcca
      301 tcagcttgag aaggtgaatt tggcaaatga catgaagatt cttcaaggca gaatccttgc
      361 agggaagctt caaaggactc catctgcaga tgttctgaaa acctctgagt ctagaaattg
      421 attattcacc caggatacct aattcaagct cccagaactc agccgaccaa ggagcttgga
      481 agactttgca actttggacc aagcacaatg aagtattccc tctgggctct gctgcttgcc
      541 gtgctgggca cacagctgct gggaagcctg tgttccaccg ttcggtccca gaggttccga
      601 ggaaggatac agcaggaacg aaaaaacatc agacccaata ttatccttgt gctcactgac
      661 gaccaggatg tggagctggg ttccctgcaa gtcatgaaca agacgagaaa gatcatggaa
      721 cagggtgggg ccaccttcac caatgccttt gtgaccacgc ccatgtgctg tccatcacgc
      781 tcatccatgc tcactgggaa gtacgtgcat aaccacaatg tctacaccaa caatgagaac
      841 tgctcgtctc cctcgtggca ggcaatgcac gagcctcgga cctttgctgt gtatctcaac
      901 aacaccggct acagaacagc cttttttgga aaatacctca atgaatacaa tggcagctac
      961 atccctcctg gatggcgaga atggctcgga ttaatcaaga attctcgttt ctataattac
     1021 actgtttgtc gcaacggcat caaggagaag catggatttg attatgcaaa ggattacttc
     1081 acagacttaa tcactaacga gagcattaat tacttcaaaa tgtctaagag aatgtatccc
     1141 cataggccca ttatgatggt gatcagccat gcagcaccac atggccctga agactccgcc
     1201 ccacagtttt caaaactgta ccccaatgcc tcccaacaca taacacccag ctataactat
     1261 gcaccgaaca tggacaaaca ctggatcatg cagtacacag ggcccatgtt gcctatccac
     1321 atggagttca ccaacgtcct ccagcgcaaa cgactgcaga ctctgatgtc agttgacgac
     1381 tctgtcgaga ggttgtacaa catgcttgtg gagagcgggg agctggacaa cacttacatc
     1441 atctacactg cggaccacgg ctaccacatt ggacagtttg ggctggtcaa ggggaaatcc
     1501 atgccctacg actttgacat ccgtgtgcct ttcttcattc gtggtccaag catagaacca
     1561 gggtcgatag tcccacagat tgttctgaac atcgacctag ctcctaccat tctggatatt
     1621 gctggtcttg actctccttc tgatgtggat ggtaaatctg tcctcaaact tctagacctg
     1681 gaaaagccgg gtaacaggtt tcggacaaac aagaaggcca aaatctggcg tgatacattc
     1741 ctagtggaaa gagggaaatt tttacggaag aaggaggaat ccggcaagaa tatccaacag
     1801 tcaaaccact tgcccaaata tgagagggtc aaggaactgt gccagcaagc cagataccag
     1861 acagcctgtg agcagcctgg gcagaactgg cagtgcatcg aggacacatc cggcaagctc
     1921 cgaatccaca agtgtaaggg acccagcgac ctgctcacgg tccgtcagaa tgcacgcaac
     1981 ctctactctc gcggattgca tgacaaagac aaagagtgcc attgtaggga ctctggctat
     2041 cgctcgagca gaagccagag aaagaatcaa aggcagttcc tgaggaacaa gggaacacca
     2101 aagtataagc ccagatttgt gcacacccgg cagactcggt ccctgtctgt tgaattcgaa
     2161 ggtgaaatat atgacatcaa cctggaagaa gaagaactgc aagtgttacc accaaggagc
     2221 attgccaagc gccatgatga gggtcaccag ggcttcattg gccaccaggc tgctgctggt
     2281 gacatcagga atgagatgtt ggcagacagc aacaatgctg tggggttacc cgccactgtc
     2341 cgggtgacac acaagtgctt tattcttcca aacgacacaa tccactgtga gagggagctg
     2401 taccagtcag ccagagcgtg gaaggaccat aaggcctaca ttgataaaga gattgaagtt
     2461 ctacaagata aaattaagaa tttaagggaa gtgaggggac acctaaagaa aaggaaacct
     2521 gaggagtgtg gctgtggtga ccagagctat tacaacaaag agaaaggtgt caaacgacag
     2581 gagaagctaa agagtcacct tcaccccttc aaggaggctg ctgcccagga ggtggatagc
     2641 aaacttcagc tcttcaagga gcatcggagg aggaagaagg agaggaagga gaagaaacgg
     2701 cagaggaagg gagaggagtg tagcctgcct ggccttacct gcttcaccca tgacaacaac
     2761 cactggcaga ctgccccatt ctggaacttg ggatctttct gtgcctgcac aagttctaac
     2821 aacaatacct actggtgttt gcgtacagtc aacgagacgc acaatttcct gttttgtgag
     2881 tttgctactg gctttctgga atatttcgac atgaatacgg atccttatca gctcacaaat
     2941 acagtacaca cagtagaacg gagcatctta aatcagctac acatacagct gatggagctc
     3001 cgaagctgcc aagggtataa acagtgcaac ccaagaccca agagcctgga cattggagct
     3061 aaagaaggag gaaactatga cccgcacaga ggacagttat gggatggatg ggaaggttag
     3121 tcagtccaat attacttcag acaccagctg gcaaggcctg gaggagttat ccggtgcaag
     3181 caacatcgat gagtacaggt ctaaccctag actaagtctg gaggactgga ctaactacct
     3241 gagggctgtc tacagagcct ttgcactgct gaacagtcac cctgatccaa acaaagcaaa
     3301 tgggactcca accacacaag gtggtgactt cctggtcacc tctgctgagc gcttggtgcc
     3361 agcagagatg gcttctgcag aatcaggtga agacccaagt catgtggttg gggaaacacc
     3421 tcctttgacc ttgccagtca acctccaaac cctgcatctg aacagaccaa cgttaagtcc
     3481 agagagaaaa cttgaatggg ataatgacat tccagaagtg aatcatttga attctgaaca
     3541 ctggagaaaa actgagaagc agataggatg ggaggagctg catcatcctg aaggtgacgt
     3601 cgtcagtggc aatggtatga cagagctgct gccccagtct catcttgggc atcagctcac
     3661 cagtcagcac caacaaaaat gttcccagga tgtggagaca gagaaggatg cttttgaaga
     3721 tcaattgcgt cctcttgtcc actctgacag aactccggtt catcgggtgt tcgatgtgtc
     3781 ccgtgtggag cagcctcttc actccagcca cacggagaga atgttggcca agatggggca
     3841 gagtgataca aaggagtggc taccaaatct cgaaggcagt gcctccgctc cagtcatctc
     3901 tgcttagatt agatgttgcc taacataata tttctttcca tgttttgatt agtaaactag
     3961 tggtggcaat catgactgcc aacattctga gcccccacct ccacccccct ccctgggcac
     4021 actcatgtgc acagcagaca ctcatgtgca cgtgtgtggg caatgctcac acagactcat
     4081 tgtaacttgc tattctacca agaagggaga ggtgggggca cattttctgt tttgatgctt
     4141 tgtttttggt actaaaacag tattatcttt tgaatatagt agggacatga gtatataaaa
     4201 tctatccagt caaaatggct agaattgtgc ctttgtaagt tttaaaagtt tgatgcccct
     4261 ggtgagtctg tcaacatatc ttctctgcct gcatagtgaa gtttgaattt gtttctaacc
     4321 actggaattt ttcaacatca tcatctttgg ttcaatgatt ttgcactttg agtttggaat
     4381 actgtgtctg cttggttggt agtgttatta tttttctttt aaacaggctt atcagagtca
     4441 cacactttgt cctaggaagg acaaaggaat agacccccgg caaggacaca cagtatggat
     4501 cacatattgt ttagcgtacg cttttcccag gaaccattgc atgtatttta cctcgacgtg
     4561 ctaaacttga ttagcagaaa ggcatggctc acaatgttgg tggtaagaaa taaatcctgc
     4621 gggaataagc aataaataaa gcaagaatga ccggctctct ctgtgcttgc tagatagacg
     4681 ctcacactgc tgacgctcgg gtgccctctt gaatgaagga acaggacaaa ggctgttttc
     4741 cttcagtctt tctccgtttc atcagtgagc gtaaacattg gaaacatacg tgtagtctta
     4801 ttattctata agacaatttt gataaactaa aatcacaaat gctggaaagt ttaagtgcac
     4861 caaaacagag gctaacttca caaacattgt gttacgttat tatttttaaa actttcgcca
     4921 agctctggtt ttctaaaaga catagttcaa aactgctttt gaaaatctgt attcttgagc
     4981 tcaccctgtt tctgttggac ttttagagac cagtcgaaga attacctcgt tgagactttt
     5041 cagtgtctcc ttcaacttca caaggtcttg ctgttgttgt ttagctcgat ggtggttttg
     5101 tttttccttc tagataattc aaacatgtag atgagttata tttttcttta agtgtttcaa
     5161 gtaaacactt ttcaagaaaa cttaatgtgg tgtagttgag tccttgctga ttttaagtct
     5221 ttaccataga gtctgttaaa cctaaccagc catttctcag atgcggatgc catctgctga
     5281 gtgacagagt aagcatattt atggtcctga ctgatgtgct gtgtgaagag attaagatat
     5341 taggaggcat actatgctct tttttttttt taatccaatg taatactctg tgatacattt
     5401 tataaaacaa ttctggcttc aggaaagtct agaagcaaca tttctggaaa taaaaggtgt
     5461 tgaaacttg
//
