LOCUS       NM_011506               2110 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus succinate-Coenzyme A ligase, ADP-forming, beta subunit
            (Sucla2), transcript variant 1, mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_011506 XM_127733
VERSION     NM_011506.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Lancaster,M.S., Kim,B., Doud,E.H., Tate,M.D., Sharify,A.D., Gao,H.,
            Chen,D., Simpson,E., Gillespie,P., Chu,X., Miller,M.J., Wang,Y.,
            Liu,Y., Mosley,A.L., Kim,J. and Graham,B.H.
  TITLE     Loss of succinyl-CoA synthetase in mouse forebrain results in
            hypersuccinylation with perturbed neuronal transcription and
            metabolism
  JOURNAL   Cell Rep 42 (10), 113241 (2023)
   PUBMED   37819759
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Tong,Y., Guo,D., Lin,S.H., Liang,J., Yang,D., Ma,C., Shao,F.,
            Li,M., Yu,Q., Jiang,Y., Li,L., Fang,J., Yu,R. and Lu,Z.
  TITLE     SUCLA2-coupled regulation of GLS succinylation and activity
            counteracts oxidative stress in tumor cells
  JOURNAL   Mol Cell 81 (11), 2303-2316 (2021)
   PUBMED   33991485
  REMARK    GeneRIF: SUCLA2-coupled regulation of GLS succinylation and
            activity counteracts oxidative stress in tumor cells.
REFERENCE   3  (bases 1 to 2110)
  AUTHORS   Zhao,Y., Tian,J., Sui,S., Yuan,X., Chen,H., Qu,C., Du,Y., Guo,L.
            and Du,H.
  TITLE     Loss of succinyl-CoA synthase ADP-forming beta subunit disrupts
            mtDNA stability and mitochondrial dynamics in neurons
  JOURNAL   Sci Rep 7 (1), 7169 (2017)
   PUBMED   28769029
  REMARK    GeneRIF: SCS A-beta loss mediates severe mitochondrial dysfunction
            including compromised OXPHOS efficiency, increased ROS generation,
            mtDNA depletion as well as altered mitochondrial dynamics towards
            fusion.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2110)
  AUTHORS   Kacso,G., Ravasz,D., Doczi,J., Nemeth,B., Madgar,O., Saada,A.,
            Ilin,P., Miller,C., Ostergaard,E., Iordanov,I., Adams,D.,
            Vargedo,Z., Araki,M., Araki,K., Nakahara,M., Ito,H., Gal,A.,
            Molnar,M.J., Nagy,Z., Patocs,A., Adam-Vizi,V. and Chinopoulos,C.
  TITLE     Two transgenic mouse models for beta-subunit components of
            succinate-CoA ligase yielding pleiotropic metabolic alterations
  JOURNAL   Biochem J 473 (20), 3463-3485 (2016)
   PUBMED   27496549
REFERENCE   5  (bases 1 to 2110)
  AUTHORS   Bowman,C.E., Zhao,L., Hartung,T. and Wolfgang,M.J.
  TITLE     Requirement for the Mitochondrial Pyruvate Carrier in Mammalian
            Development Revealed by a Hypomorphic Allelic Series
  JOURNAL   Mol Cell Biol 36 (15), 2089-2104 (2016)
   PUBMED   27215380
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2110)
  AUTHORS   Donti,T.R., Stromberger,C., Ge,M., Eldin,K.W., Craigen,W.J. and
            Graham,B.H.
  TITLE     Screen for abnormal mitochondrial phenotypes in mouse embryonic
            stem cells identifies a model for succinyl-CoA ligase deficiency
            and mtDNA depletion
  JOURNAL   Dis Model Mech 7 (2), 271-280 (2014)
   PUBMED   24271779
  REMARK    GeneRIF: Sucla2(SAbetageo) homozygotes exhibited recessive
            lethality, with most mutants dying late in gestation (e18.5).
            Mutant placenta and embryonic (e17.5) brain, heart and muscle
            showed varying degrees of mitochondrial DNA depletion.
REFERENCE   7  (bases 1 to 2110)
  AUTHORS   Lambeth,D.O., Tews,K.N., Adkins,S., Frohlich,D. and Milavetz,B.I.
  TITLE     Expression of two succinyl-CoA synthetases with different
            nucleotide specificities in mammalian tissues
  JOURNAL   J Biol Chem 279 (35), 36621-36624 (2004)
   PUBMED   15234968
  REMARK    GeneRIF: ADP and GDP succinyl-CoA synthetases have different
            expression patterns and specificity
REFERENCE   8  (bases 1 to 2110)
  AUTHORS   Johnson,J.D., Mehus,J.G., Tews,K., Milavetz,B.I. and Lambeth,D.O.
  TITLE     Genetic evidence for the expression of ATP- and GTP-specific
            succinyl-CoA synthetases in multicellular eucaryotes
  JOURNAL   J Biol Chem 273 (42), 27580-27586 (1998)
   PUBMED   9765291
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113198.15 and AC155246.1.
            
            On Mar 29, 2018 this sequence version replaced NM_011506.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK160130.1, BC057605.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-112               AC113198.15        19203-19314         c
            113-293             AC113198.15        11479-11659         c
            294-393             AC113198.15        3367-3466           c
            394-556             AC113198.15        3076-3238           c
            557-685             AC155246.1         115027-115155       c
            686-824             AC155246.1         112694-112832       c
            825-986             AC155246.1         103593-103754       c
            987-1129            AC155246.1         103359-103501       c
            1130-1250           AC155246.1         101759-101879       c
            1251-1339           AC155246.1         100746-100834       c
            1340-2110           AC155246.1         98367-99137         c
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 39.06 cM"
     gene            1..2110
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /note="succinate-Coenzyme A ligase, ADP-forming, beta
                     subunit"
                     /db_xref="GeneID:20916"
                     /db_xref="MGI:MGI:1306775"
     exon            1..112
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..1414
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /EC_number="6.2.1.5"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; succinyl-CoA ligase [ADP-forming] subunit beta,
                     mitochondrial; succinyl-CoA synthetase beta-A chain;
                     ATP-specific succinyl-CoA synthetase subunit beta;
                     succinate--CoA ligase [ADP-forming] subunit beta,
                     mitochondrial"
                     /codon_start=1
                     /product="succinate--CoA ligase [ADP-forming] subunit
                     beta, mitochondrial isoform 1 precursor"
                     /protein_id="NP_035636.1"
                     /db_xref="CCDS:CCDS27271.1"
                     /db_xref="GeneID:20916"
                     /db_xref="MGI:MGI:1306775"
                     /translation="MAASMFYGRQLAAAALRSHRPQTTLRAAAQVLGNSGLFNKHGLQ
                     VQQQQQRTLSLHEYLSMELLQEAGVSVPKGFVAKSSDEAYAIAKKLGSKDVVIKAQVL
                     AGGRGKGTFTSGLKGGVKIVFSPEEAKAVSSQMIGQKLITKQTGEKGRICNQVLVCER
                     KYPRREYYFAITMERSFQGPVLIGSAQGGVNIEDVAAENPEAIVKEPIDIVEGIKKEQ
                     AVTLAQKMGFPSNIVDSAAENMIKLYNLFLKYDATMVEINPMVEDSDGKVLCMDAKIN
                     FDSNSAYRQKKIFDLQDWSQEDERDKEAANADINYIGLDGSIGCLVNGAGLAMATMDI
                     IKLHGGTPANFLDVGGGATVQQVTEAFKLITSDKKVQAILVNIFGGIMRCDVIAQGIV
                     MAVKDLEIRIPVVVRLQGTRVDDAKALIADSGLKILACDDLDEAAKMVVKLSEIVTLA
                     KEAHVDVKFQLPI"
     exon            113..293
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /inference="alignment:Splign:2.1.0"
     exon            294..393
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /inference="alignment:Splign:2.1.0"
     exon            394..556
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /inference="alignment:Splign:2.1.0"
     exon            557..685
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /inference="alignment:Splign:2.1.0"
     exon            686..824
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /inference="alignment:Splign:2.1.0"
     exon            825..986
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1129
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1250
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1339
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..2110
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1592..1597
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /note="hexamer: TATAAA"
     polyA_site      1617
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /note="major polyA site"
     regulatory      1732..1737
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /note="hexamer: AATAAA"
     polyA_site      1754
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
     regulatory      2090..2095
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /note="hexamer: AATAAA"
     polyA_site      2110
                     /gene="Sucla2"
                     /gene_synonym="4930547K18Rik; A-SCS; SCS-betaA"
                     /note="major polyA site"
ORIGIN      
        1 aggggacggg tcggactcag aaatggcggc gtccatgttc tacggccggc agttggccgc
       61 ggccgccctg aggagccacc ggccgcagac tacgctgcga gccgccgccc aggttctggg
      121 aaattctggc ttgtttaaca agcatggact acaagtgcag cagcagcagc agaggaccct
      181 ttcgctgcat gaatacttga gtatggagtt attgcaggaa gcgggtgtct ctgttcccaa
      241 aggcttcgtg gcaaagtcat ccgatgaagc ttacgcaatt gccaaaaaat taggatcaaa
      301 ggatgttgtg ataaaggcac aggttttggc tggcggcaga ggaaaaggaa catttacaag
      361 tggcctcaaa ggaggagtga agattgtttt ctctccagaa gaagcaaaag ctgtttcctc
      421 acagatgatt gggcaaaaat tgattaccaa gcaaactgga gaaaagggca gaatatgcaa
      481 tcaagttttg gtctgtgagc gcaaatatcc caggagagaa tactactttg caataacaat
      541 ggagaggtca tttcaaggtc ctgtactgat aggaagtgca caaggtggtg ttaacattga
      601 agatgtggct gccgagaacc ctgaagccat tgtaaaggag cctattgata tagtggaggg
      661 catcaaaaag gaacaagccg tgacacttgc acagaagatg ggatttcctt ctaacattgt
      721 ggactctgca gcagaaaaca tgatcaagct ttataatctt tttctgaagt atgatgcaac
      781 tatggtagaa ataaacccaa tggtagaaga ttctgacgga aaggtgttgt gcatggatgc
      841 aaagatcaat tttgattcaa attcagccta ccgccagaag aagatctttg atcttcagga
      901 ctggagtcag gaagacgaaa gggacaaaga agcagctaat gcagatatta actacatcgg
      961 cctcgatggg agcataggat gtctagtaaa tggtgctggc ttggctatgg ccacaatgga
     1021 tataataaaa ctacacggag ggactccagc caactttctt gatgttggtg gaggcgccac
     1081 agtccagcaa gtaacagaag cgtttaaact catcacttca gataaaaagg tgcaggctat
     1141 tctggtcaac atttttggag gaatcatgag atgtgatgtt attgcacaag gaatagtcat
     1201 ggcagtaaag gatttagaaa tcagaatacc tgttgtggtg cggttacaag gtacacgagt
     1261 tgatgatgct aaggcactaa tagcagacag tggtcttaaa atccttgctt gtgatgattt
     1321 ggatgaagct gctaaaatgg ttgtaaagct ctctgaaata gtgaccttag ccaaagaagc
     1381 ccatgtggat gtgaagtttc aattgccaat atgagcaaga ccttggtgga tggctacggg
     1441 tattaatgtg ctgtaattct ttattatact gtggtctctc ctagtgtccc ttttctttta
     1501 atgtgtggag aatgtaatca ccacataggc acacatacct ttgaaagcat ttgctctgca
     1561 tctgattctg ctgctgagaa tggactgttt atataaagaa tgtctgatat aaacttacgg
     1621 tggtctcttt tcttgcagcc atcttttttt gcttcctcta cagaatgtca cttgcagtat
     1681 gcctgctttc tggtgttgga tacagagttc ttcattgata agagttctat aaataaaatt
     1741 actacaaaga taaagcttta ttctttagta taaaaatata tctaataaca gcctcattag
     1801 gacaatggta tgttataaca atgttttata taagaaatat ttatcttcag catcaaatgg
     1861 ctcttaaatg tatcaatcat tatttataca tagaacttgg aagcactcct cagaacatct
     1921 tttaagtatt ttagagaagt aacttgctga ttatttgaac atcattttaa ttattacagg
     1981 acaatgataa gtgcaagtcg tcatgattct gtggtggtaa aaacacacgc agattgataa
     2041 gaaaggcacc tattaagact tggacaaaac tagaatgcca gcttaagtca ataaaagttt
     2101 cctaatgtaa
//
