LOCUS       NM_001408063            9370 bp    mRNA    linear   ROD 07-AUG-2024
DEFINITION  Mus musculus syntaxin binding protein 5 (tomosyn) (Stxbp5),
            transcript variant 2, mRNA.
ACCESSION   NM_001408063 XM_006512926
VERSION     NM_001408063.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 9370)
  AUTHORS   Subkhangulova,A., Gonzalez-Lozano,M.A., Groffen,A.J.A., van
            Weering,J.R.T., Smit,A.B., Toonen,R.F. and Verhage,M.
  TITLE     Tomosyn affects dense core vesicle composition but not exocytosis
            in mammalian neurons
  JOURNAL   Elife 12, e85561 (2023)
   PUBMED   37695731
  REMARK    GeneRIF: Tomosyn affects dense core vesicle composition but not
            exocytosis in mammalian neurons.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9370)
  AUTHORS   Rahman,M.M., Pathak,A., Schueler,K.L., Alsharif,H., Michl,A.,
            Alexander,J., Kim,J.A. and Bhatnagar,S.
  TITLE     Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to
            increase insulin secretion from pancreatic beta-cells improving
            glucose clearance
  JOURNAL   FASEB J 37 (8), e23075 (2023)
   PUBMED   37432648
  REMARK    GeneRIF: Genetic ablation of synaptotagmin-9 alters tomosyn-1
            function to increase insulin secretion from pancreatic beta-cells
            improving glucose clearance.
REFERENCE   4  (bases 1 to 9370)
  AUTHORS   Geerts,C.J., Mancini,R., Chen,N., Koopmans,F.T.W., Li,K.W.,
            Smit,A.B., van Weering,J.R.T., Verhage,M. and Groffen,A.J.A.
  TITLE     Tomosyn associates with secretory vesicles in neurons through its
            N- and C-terminal domains
  JOURNAL   PLoS One 12 (7), e0180912 (2017)
   PUBMED   28746398
  REMARK    GeneRIF: Data indicate that bidirectional moving tomosyn-1 (Stxbp5)
            and tomosyn-2 (Stxbp5l) puncta were observed.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9370)
  AUTHORS   Zhu,Q.M., Ko,K.A., Ture,S., Mastrangelo,M.A., Chen,M.H.,
            Johnson,A.D., O'Donnell,C.J., Morrell,C.N., Miano,J.M. and
            Lowenstein,C.J.
  TITLE     Novel Thrombotic Function of a Human SNP in STXBP5 Revealed by
            CRISPR/Cas9 Gene Editing in Mice
  JOURNAL   Arterioscler Thromb Vasc Biol 37 (2), 264-270 (2017)
   PUBMED   28062498
REFERENCE   6  (bases 1 to 9370)
  AUTHORS   Zhang,W., Lilja,L., Mandic,S.A., Gromada,J., Smidt,K., Janson,J.,
            Takai,Y., Bark,C., Berggren,P.O. and Meister,B.
  TITLE     Tomosyn is expressed in beta-cells and negatively regulates insulin
            exocytosis
  JOURNAL   Diabetes 55 (3), 574-581 (2006)
   PUBMED   16505218
  REMARK    GeneRIF: In the pancreatic beta-cell, tomosyn negatively regulates
            insulin exocytosis.
REFERENCE   7  (bases 1 to 9370)
  AUTHORS   Kanda,H., Tamori,Y., Shinoda,H., Yoshikawa,M., Sakaue,M.,
            Udagawa,J., Otani,H., Tashiro,F., Miyazaki,J. and Kasuga,M.
  TITLE     Adipocytes from Munc18c-null mice show increased sensitivity to
            insulin-stimulated GLUT4 externalization
  JOURNAL   J Clin Invest 115 (2), 291-301 (2005)
   PUBMED   15690082
  REMARK    GeneRIF: The interaction between syntaxin4 and Munc18c in
            adipocytes results in enhancement of insulin-stimulated GLUT4
            externalization.
REFERENCE   8  (bases 1 to 9370)
  AUTHORS   Groffen,A.J., Jacobsen,L., Schut,D. and Verhage,M.
  TITLE     Two distinct genes drive expression of seven tomosyn isoforms in
            the mammalian brain, sharing a conserved structure with a unique
            variable domain
  JOURNAL   J Neurochem 92 (3), 554-568 (2005)
   PUBMED   15659226
REFERENCE   9  (bases 1 to 9370)
  AUTHORS   Widberg,C.H., Bryant,N.J., Girotti,M., Rea,S. and James,D.E.
  TITLE     Tomosyn interacts with the t-SNAREs syntaxin4 and SNAP23 and plays
            a role in insulin-stimulated GLUT4 translocation
  JOURNAL   J Biol Chem 278 (37), 35093-35101 (2003)
   PUBMED   12832401
  REMARK    GeneRIF: Interacts with SNAP23 and Syntaxin4 and plays a role in
            Insulin-stimulated GLUT4 translocacion.
REFERENCE   10 (bases 1 to 9370)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122390.4.
            
            On Jun 9, 2022 this sequence version replaced XM_006512926.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-692               AC122390.4         148308-148999       c
            693-790             AC122390.4         146934-147031       c
            791-872             AC122390.4         114390-114471       c
            873-973             AC122390.4         112790-112890       c
            974-1108            AC122390.4         91004-91138         c
            1109-1172           AC122390.4         89648-89711         c
            1173-1256           AC122390.4         85854-85937         c
            1257-1380           AC122390.4         83611-83734         c
            1381-1459           AC122390.4         73147-73225         c
            1460-1614           AC122390.4         65083-65237         c
            1615-1687           AC122390.4         64028-64100         c
            1688-1835           AC122390.4         60037-60184         c
            1836-1903           AC122390.4         59742-59809         c
            1904-1944           AC122390.4         59445-59485         c
            1945-2165           AC122390.4         56806-57026         c
            2166-2344           AC122390.4         56197-56375         c
            2345-2470           AC122390.4         47720-47845         c
            2471-2622           AC122390.4         45899-46050         c
            2623-2688           AC122390.4         37140-37205         c
            2689-2748           AC122390.4         32118-32177         c
            2749-2796           AC122390.4         31965-32012         c
            2797-2847           AC122390.4         25798-25848         c
            2848-3087           AC122390.4         18227-18466         c
            3088-3457           AC122390.4         17118-17487         c
            3458-3623           AC122390.4         16607-16772         c
            3624-3735           AC122390.4         14935-15046         c
            3736-3956           AC122390.4         10522-10742         c
            3957-9370           AC122390.4         3318-8731           c
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 3.21 cM"
     gene            1..9370
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /note="syntaxin binding protein 5 (tomosyn)"
                     /db_xref="GeneID:78808"
                     /db_xref="MGI:MGI:1926058"
     exon            1..692
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     CDS             540..3998
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /note="isoform 2 is encoded by transcript variant 2;
                     syntaxin-binding protein 5; tomosyn-1; lethal(2) giant
                     larvae protein homolog 3"
                     /codon_start=1
                     /product="syntaxin-binding protein 5 isoform 2"
                     /protein_id="NP_001394992.1"
                     /db_xref="GeneID:78808"
                     /db_xref="MGI:MGI:1926058"
                     /translation="MRKFNIRKVLDGLTAGSSSASQQQQQQQHPPGNREPEIQETLQS
                     EHFQLCKTVRHGFPYQPSALAFDPVQKILAVGTQTGALRLFGRPGVECYCQHDSGAAV
                     IQLQFLINEGALVSALADDTLHLWNLRQKRPAVLHSLKFCRERVTFCHLPFQSKWLYV
                     GTERGNIHIVNVESFTLSGYVIMWNKAIELSSKAHPGPVVHISDNPMDEGKLLIGFES
                     GTVVLWDLKSKKADYRYTYDEAIHSVAWHHEGKQFICSHSDGTLTIWNVRSPAKPVQT
                     ITPHGKQLKDGKKPEPCKPILKVELKTTRSGEPFIILSGGLSYDTVGRRPCLTVMHGK
                     STAVLEMDYSIVDFLTLCETPYPNDFQEPYAVVVLLEKDLVLIDLAQNGYPIFENPYP
                     LSIHESPVTCCEYFADCPVDLIPALYSVGARQKRQGYSKKEWPINGGNWGLGAQSYPE
                     IIITGHADGSVKFWDASAITLQVLYKLKTSKVFEKSRNKDDRQNTDIVDEDPYAIQII
                     SWCPESRMLCIAGVSAHVIIYRFSKQEVLTEVIPMLEVRLLYEINDVDTPEGEQPPPL
                     STPVGSSNPQPIPPQSHPSTSSSSSDGLRDNVPCLKVKNSPLKQSPGYQTELVIQLVW
                     VGGEPPQQITSLALNSSYGLVVFGNCNGIAMVDYLQKAVLLNLSTIELYGSNDPYRRE
                     PRSPRKSRQPSGAGLCDITEGTVVPEDRCKSPTSGSSSPHNSDDEQKVNNFIEKVKTQ
                     SRKFSKMVANDLAKMSRKLSLPTDLKPDLDVKDNSFSRSRSSSVTSIDKESRETISAL
                     HFCETLTRKADSSPSPCLWVGTTVGTAFVITLNLPPGPEQRLLQPVIVSPSGTILRLK
                     GAILRMAFLDATGCLMSPAYEPWKEHNVAEEKDEKEKLKKRRPVSVSPSSSQEISENQ
                     YAVICSEKQAKVMSLPTQSCAYKQNITETSFVLRGDIVALSNSVCLACFCANGHIMTF
                     SLPSLRPLLDVYYLPLTNMRIARTFCFANNGQALYLVSPTEIQRLTYSQETCENLQEM
                     LGELFTPVETPEAPNRGFFKGLFGGGAQSLDREELFGESSSGKASRSLAQHIPGPGGI
                     EGVKGAASGVVGELARARLALDERGQKLSDLEERTAAMMSSADSFSKHAHEMMLKYKD
                     KKWYQF"
     exon            693..790
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            791..872
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            873..973
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1108
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1172
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1256
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1380
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..1459
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1614
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..1687
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1835
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1903
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            1904..1944
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..2165
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            2166..2344
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            2345..2470
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            2471..2622
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            2623..2688
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            2689..2748
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            2749..2796
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            2797..2847
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            2848..3087
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            3088..3457
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            3458..3623
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            3624..3735
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            3736..3956
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     exon            3957..9370
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4314..4319
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /note="hexamer: ATTAAA"
     polyA_site      4335
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /note="major polyA site"
     regulatory      4389..4394
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /note="hexamer: AATAAA"
     polyA_site      4412
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /note="major polyA site"
     regulatory      7134..7139
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /note="hexamer: AATAAA"
     polyA_site      7156
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /note="major polyA site"
     regulatory      9337..9342
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
                     /note="hexamer: AATAAA"
     polyA_site      9370
                     /gene="Stxbp5"
                     /gene_synonym="0710001E20Rik; 4930565N16Rik; LGL3;
                     mKIAA1006"
ORIGIN      
        1 agctcgctcc gcggaggccc agctgccgcc accgccgcgg ccggcgctgg aaccgcggct
       61 cgccgcctgc tctaccgagc gttgagggcc agggtccgag gctcacggga ggcgacagcg
      121 agcatccggg gagagagcga caggcggcgc ggagaccggc cccgggtgcc cgggccatgc
      181 gggccgggcg gctgcgctga acggaggccg agcccgggac ctgccggggg actgccgggc
      241 tgcgggccat ggatgcggcg gcagcggcgc gggacggcgg gagcccggcc gcgaccaggt
      301 gaggaggcgg cgtccggcgc cactgcagcc gcggcggcct cggaggaaga aggctcgccg
      361 ccgcgcgccc gcccgcccgt cgtcgcagcc cttcctgttg ggattatctt ctgctccccg
      421 ctgctccttc gctccccgcg gtcgaagccg ccgctcggct tcagcggctc ggactccttc
      481 gcagccggtg cctcctcccc tacctgggcc tcgcagcggg gagacccttg ggcgagacca
      541 tgaggaaatt caacatcagg aaggtgctgg acggcctgac cgcaggctcg tcctcggcct
      601 cgcaacagca gcaacagcag cagcacccgc ctgggaaccg ggagccggag atccaggaga
      661 cgctccagtc cgaacacttc caactctgca agactgttcg ccatggattc ccctatcagc
      721 cctcagccct ggcctttgat cccgttcaga agatcctggc ggtaggaact cagactggtg
      781 ctttaaggct ctttggtcgt ccaggagtgg aatgttattg ccagcacgac agcggagcag
      841 cagtgatcca actccagttc ctgattaatg agggagccct tgtgagtgcc ttggctgatg
      901 acaccctaca cttatggaat ttacgtcaaa aaaggcctgc cgtactacat tcacttaaat
      961 tttgcagaga aagggttaca ttttgccatc tgcctttcca gagtaagtgg ctctatgtgg
     1021 gcactgagcg aggaaacata cacattgtta atgtggagtc cttcacactc tcaggctatg
     1081 tcattatgtg gaataaagcc atcgaactgt catctaaagc tcacccagga ccagttgtgc
     1141 atataagtga caatcccatg gacgagggga agcttttgat tggcttcgaa tctggaacag
     1201 tagtcttatg ggacctcaag tcaaagaagg ctgactacag atacacatac gacgaggcta
     1261 ttcactctgt ggcttggcat catgaaggaa aacaatttat ttgcagtcat tctgacggta
     1321 ctttgaccat atggaatgtg agatcccctg ctaaacctgt acagaccatc actcctcacg
     1381 gaaaacagtt gaaggatggg aagaagccag agccatgcaa gcctatcctg aaggtggagt
     1441 tgaagacaac aagatccggg gaacctttta ttattttgtc aggaggctta tcctatgata
     1501 ccgtgggaag aagaccctgc ttaacagtga tgcacgggaa aagcacagca gtgctggaaa
     1561 tggactattc aatcgttgac tttctcacac tctgtgaaac gccatatcca aacgattttc
     1621 aggaaccata tgctgtggtt gttctcctgg aaaaggactt agtgctgata gaccttgcac
     1681 aaaatggata ccctatattt gagaatccct accctttgag tatacacgag tcccctgtta
     1741 catgttgtga atattttgct gattgtcctg tggatcttat tcctgcactt tactctgttg
     1801 gagctagaca gaagcgtcaa ggttacagca aaaaggaatg gcccatcaat ggtggtaatt
     1861 ggggcttggg tgctcaaagt tacccagaaa taattattac agggcatgct gatggctcgg
     1921 tgaaattctg ggacgcttct gcaataactc tacaagtact atataaatta aaaacatcta
     1981 aagtatttga gaagtcaaga aacaaagatg acagacagaa cacagacatt gtagatgaag
     2041 atccatatgc cattcagatc atctcctggt gcccagagag caggatgctg tgcatagctg
     2101 gagtgtcagc ccatgtcatc atctatagat tcagcaagca ggaagtgctt accgaagtca
     2161 tcccgatgct tgaagttcga ctgttatatg aaataaatga tgtggatact ccagagggtg
     2221 agcagccccc gcctctgtcc acccccgtgg gcagctccaa ccctcagccc attcctcctc
     2281 agtctcatcc gtctaccagc agcagctcat ctgacgggct tcgtgataac gttccttgct
     2341 taaaagttaa aaactcccca cttaagcagt ctccaggcta tcagacagag ctggtcattc
     2401 agttggtgtg ggtgggtgga gaacccccgc agcagatcac cagcctggca ctcaactctt
     2461 cctatggact ggtggttttc ggcaactgta acggcatcgc aatggttgac tacctccaga
     2521 aagcagtact gctcaacctc agcaccatcg aactttacgg ctcaaatgat ccttatcgga
     2581 gagaacctcg ctcgccccga aaatcccgac agccttcagg agcgggcctg tgtgatatca
     2641 ccgaagggac tgtcgtcccg gaggatcgct gcaaatctcc cacttccggt tcttcatcgc
     2701 cacacaattc agatgatgaa caaaaagtga ataattttat agaaaaggtg aagacccaaa
     2761 gcagaaagtt ttccaagatg gtagccaatg atctagcaaa gatgtcaagg aaattaagct
     2821 tgccaactga cctaaagcct gatttagatg taaaagacaa ttccttcagc agatcgcgga
     2881 gttcaagtgt aaccagcatt gacaaagagt cccgggaaac catctctgct cttcatttct
     2941 gtgagacttt gacgcggaag gccgactctt ccccctcccc ctgcctgtgg gtcggaacta
     3001 cagtgggaac tgcgtttgtc atcacgctga atctcccccc ggggcctgag cagagactcc
     3061 ttcagccagt gattgtgtct ccaagcggta ctatattgag gttaaaaggt gcaatcttga
     3121 ggatggcatt tctggatgcc acgggctgct taatgtctcc tgcatacgaa ccctggaaag
     3181 aacacaacgt tgctgaagaa aaagacgaaa aggagaaatt gaaaaagcgg cgacctgtct
     3241 cagtgtcccc ctcctcttct caggaaatta gtgagaacca gtacgcagtg atatgctctg
     3301 aaaagcaagc aaaggtcatg tcactgccaa cccagagctg tgcatacaag cagaacatca
     3361 ccgagacgtc cttcgtgctc cgcggagaca ttgtcgcact gagtaacagt gtctgcctcg
     3421 cctgcttctg tgccaatggc catatcatga ctttcagttt gccaagcttg aggcctctcc
     3481 tggatgtcta ctacctgccc cttaccaaca tgcggatagc caggacattc tgcttcgcca
     3541 acaacggaca agccttatac cttgtttcac ctacagaaat ccagagactc acctacagtc
     3601 aggagacatg tgaaaatctt caggagatgc tcggtgaact cttcacgcct gtagagacac
     3661 cagaagcacc aaacagaggc ttcttcaaag gcttatttgg aggcggtgca caatctctcg
     3721 atagagaaga actatttgga gagtcgtcct cgggaaaggc ctccaggagc ctcgcacaac
     3781 acatcccggg ccctggtggg atcgaaggtg tgaaaggagc cgcgtcagga gtggtgggtg
     3841 aacttgcccg agccaggctg gccctcgacg aaagaggaca gaagctcagt gacttggaag
     3901 aaaggacggc agccatgatg tccagcgcag actccttctc caaacatgct catgagatga
     3961 tgctgaaata caaagataag aagtggtacc agttctgaca actagaactc agtaagtcca
     4021 gcttcaacca gaaggaaaaa gaagtttcct tgttgatatc actgatgtat ttggggaaga
     4081 taacgtaaaa gggacgcact ttgctgagaa cttctttccc agcacaatca tgcactgttt
     4141 tacctcagtc acacggcttt aactgagaag actccccatg cgtgcaaacc ggagcataga
     4201 gtgtgtgctg gctgtgcatt gacgaacgga gaactgagtg gggtcacgct gacagatgaa
     4261 catccagaag atgttgacaa gtcctggagg actattctgt ggatcattcc tgtattaaac
     4321 tttcatatgc caaaagactt tgtgccgttg tatctgccat cagtgtttga tccgacgtgg
     4381 ggagaggcaa taaaccagaa gttgtaagtc cacaaaggtc actttgtatc tggactgcat
     4441 tgtgagcagc tgtctgcact caccctctct caaacagact gcttatcagt cttagtgaaa
     4501 aagcagcaag ctgtcccttc tcgctagact ctgccgaatg gtgggctttc agtaatcaga
     4561 gcacacacac acagctcgtg tgcgtgcggg atcctcttct actctcatca tcacatactg
     4621 tgttcagttc ctatgttttc caaagcaaaa caaggtgatg tgtcactttt catggaaaag
     4681 tgtggcgcat agctgagtcg ctcatcttcg gggagtttcc atgtagtagc catgcagagt
     4741 ggaagagtga gagaacctgc attgtggtga ggagaatccg tccctgtcct gtgtcctgtt
     4801 cacatcagtt cagctaaaag tagacctgac caacacagtt actggttaaa tttgtagaaa
     4861 agtcgaaatt ggctaagttt ttaattttgc ttgaaatttt ataaaatgtt taaccaaatg
     4921 tacccttgca ttatttaatt aaaattgcta aaacagcagt gttttatcat ttcagtaaag
     4981 tgctcagctc ccttttaaaa ctgccctcca ttcgctcaca gttccagtac actcaggtga
     5041 tgagccagtt taatctcagt gcacacactg ctgccgcatg gagagctgca gggctctctt
     5101 gtccagttat ccaagagtct agtctgatga gctacaaaat atctcccgta gaaaacatca
     5161 cgctcctgta catatccctt tgacatacta aaagcaaaaa ggaaatgttg acagcagtca
     5221 gtcatgaagt tttttctgta accaagagtg gtgtgcattg gagggcagca gacactgagt
     5281 gattggttct tgcagtagca tgacagccta gagaggatgc agcctgtact gcagtcaggg
     5341 gctgtgattg ctaaagccac aaaggaagcc gggaagggaa ggggagtggg ggtgtgaact
     5401 tcacacactg tctcctgtgg acgtctgtac tgggtcactg tccttacgtg caggcagggc
     5461 acagattcct gcgactcggg ctttttcctg ctctgtgtga gaagttaagt ccctgtaggt
     5521 catcctcact tctcagcctg ccgcttgccc ctgtctcttt cattaataat tcttttgttt
     5581 acattttata atgtgcacta ctggatatca aagtttacat caaagtttac tttaaatagc
     5641 atagtaagga ttaaatatat gtgataaaga gaactgatca agataaaaat gtttttaaac
     5701 tataatcatt ttttaaaatt tctgaatctg tagaaagtga aaaaacattc gtttcatgtc
     5761 tgttctctga acattcggca atttacaaat tgcttaattt gcattatata ttgtccatta
     5821 aacccatgtt ccatggtacc tcactggaag aattgggtcc tttcttaaag gtatagtttt
     5881 atgaatatcg atagtcgaga gttttaaaaa gttgaaaagt actaagtacc taacacactg
     5941 cttgactctg tgtggagcct cagctgttta atttgttggg ttgtttgcat atcaggttct
     6001 tttctaccgt cattttaacc gctgtaaata gatgagtcaa agttgtgatt atttactaat
     6061 agagaagtag agaagtttac cggagttgga tttttataag aacattttaa ttcattttaa
     6121 attatttgtt ctttatttta attgatctac atattgtgca caacaggcta agagaccagt
     6181 actagaaagt acaaagtatt tttaagtaga gaaaatgagc ttcccagggc tgcacatgac
     6241 tatgtaaata agacagttcc cagtgataac tggcccaggg catggactgg tctgaaatgg
     6301 cctgcctcct cccaacccac tcctgtcttt ttgatgcaaa aagcagtagc caccagcaag
     6361 ccaaagtttc aggaacttta acaataacaa caaaaggtca aaaactaaaa tatcaaaaac
     6421 ataaaagagt gtgtgagtac gtgtgtgtct tctctgtaaa ctcatttgtc tttgattctg
     6481 aaattcaagt attcttcagt taattttatt aatgggaaga gattgtctct atgtaccaca
     6541 taacaaactt gtttcatttt gcaaactaaa atcagtccct gtttgcttag aatgttgtta
     6601 tttgaagtat ctcattttgt caaaatcttt atgtgtttga acatggcgtc accttaatac
     6661 ctgctcacat tacggccata ccacttccaa aaactggtta gagatttaac aaaacctgtc
     6721 cctgatttga gattttcaca ggaaaatact gctatgtaca tctgcatatc attgcaagta
     6781 tgctgcagtt ttatccagaa tgcttcctta agacctcact tagaacatcc tgtgggttct
     6841 tctgctgtta gttcaggtag acatttgtgg aaggcagcaa gacgggtctg aaagagcaca
     6901 catgtttaga gtgtgggaga tgctgggaga cgggagagga acaggggggc ttgttccaag
     6961 ttttacccta agttctgtat tcaaatcttt gctcaaggca tttcgcatgt gacattagta
     7021 aagctatctc caaaggtttt tgtttgtttg tttgtttttg tatctgtttt tcttttttgt
     7081 ggcaaagatt tattttaact tgggggattt tgtttgtttc tatgtttctg caaaataaag
     7141 agcactgaac tttaaacttg aatttttctg cactttctta ggtcatgact ttttattgtc
     7201 ctttatttaa tctgcacttt ttcggtttaa atcaagtgat acatgtgtct gagacatacc
     7261 aaaatcgtga ctacgctgct agcgtcacct aaaaccaatc ggttctaaaa cctttagtag
     7321 ggctttgcct agcttttaaa gattaaaaac aaatgaaatg atttgctgtg tgtttcgatg
     7381 ggcttgatct ttgttgcgac actttcggag ttgcttttat aatcacataa aaacatgcta
     7441 tgtttatatg ctgctagaaa gtatactttt agttttagat gaatttttat gaatgtactc
     7501 tttaatttta aaaatggtga acttgttaag tcaaagtact taaaaagtat gtatattatc
     7561 catataaaaa gttatgtttt acccttatac taagaaatac tggtatctca tttgagatac
     7621 atttaagttt taaaaggtca tgcattattg aaaggccttc acacctgaca aaacacagtt
     7681 atgtagttaa aaaaaaaaac tctgccaaat attttccaat attgatcttt ttgaggggaa
     7741 atgtccacaa ccataatttc tagacctttt ctgaagcttc agcttgctat tggatgccta
     7801 ttttagttat tgttttaaaa tacatatata tatacatata ttgaaaatgg ttttccagat
     7861 attttcttct acctttttta aaaataaatt tccaaaaata gaaacagagt ttatgtattt
     7921 ttgtcaatga aggttttttc tttttatttt aatagtttat agagtttgtt ccttatcagt
     7981 ttgatactta atcaatactc ctaggccttt ttattctgta tttactttga aaagaacact
     8041 cctatgtagc cagtatccct tctggttttc tgtttgtctt tttctttcag tgtttgttct
     8101 ggggtgagat tgaagcccaa gtgtgctgct gccttggaga tgactatgca gcggctgagc
     8161 tttgaccttt gtccgtctgt ctctccttcc tctggcccgc tgtagagtaa tgtgggcacc
     8221 atcgctcttg cttcagatgc tgtgagcatt ctgtgaacgg aagacagttg ctgcaaatga
     8281 aaggggcaaa gctgtagcgt agcttcctgg gaaagtgaat gattttaata acttcccagt
     8341 ctgggtctga agttactggt tcagtttttc tatgaattca gtattgtctc cagagttgct
     8401 tacgggtggc ctctttctgt tacgtgcagg aaatgggatt atatatctgc aagtattttt
     8461 cagtgaggca catagtttaa tctttataag gtataaatag tgtcaactca ttgtaggtca
     8521 tttgactcag gcatttaaaa tgggtgtagg ttaattgtgg gattttattt atgtgagttt
     8581 ggggttttga ttattttttt atagcacttg tattttgtta tccaaacttt gctccaattg
     8641 aagagagtgt actgtgtttc tgacagactt agatgcacta agaagtaatg aaaagatgag
     8701 aaatagtggc atcatttctt tccttctttc ttttcttttt ttgtaaatag ctatgtgata
     8761 ctttaggtgt gacctctact taacatggta gctagtctag ggaggggagc actgtctagt
     8821 tacacttcct ttcattgtaa acgtgtttca aaaaaaaaaa cagttataaa acagacagac
     8881 ttagtttcct agggttgtcc atgcggaggg caagcatgca tccagactgc tagcctgtct
     8941 gtcaacaccg tacgcactgt tcatctcctg cattttctct ttacgttcgc tcagtgcaga
     9001 actgtttcta aaataattcc tggaatgtgc ttttggagta cagttttttt taattatctg
     9061 tgtagtgttt catttttttt taagaaaaaa aaaagcacta ttgcaaaccc tgaaaaacat
     9121 cctcagactt tgtagttctt ttgcgtttct gtaacttaat gagaaaaaaa aaatactgtc
     9181 atcacagcaa ataggttcaa aattaggtga tgaaatattt ttgtaaataa ataccattaa
     9241 gctggtattt tgagaatgta ttacaaatta atgtttctga gaaatgttcc tatatatgta
     9301 aatgaatttc tttgctgaag agctctgatt gggcaaaata aaatactcta atctcttctg
     9361 aaacaaaaaa
//
