LOCUS       NM_018768               4148 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus syntaxin 8 (Stx8), transcript variant 1, mRNA.
ACCESSION   NM_018768 XM_917120 XM_924682 XM_924689 XM_924696 XM_924704
            XM_924708 XM_924711 XM_924713
VERSION     NM_018768.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4148)
  AUTHORS   Castonguay,J., Orth,J.H.C., Muller,T., Sleman,F., Grimm,C.,
            Wahl-Schott,C., Biel,M., Mallmann,R.T., Bildl,W., Schulte,U. and
            Klugbauer,N.
  TITLE     The two-pore channel TPC1 is required for efficient protein
            processing through early and recycling endosomes
  JOURNAL   Sci Rep 7 (1), 10038 (2017)
   PUBMED   28855648
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4148)
  AUTHORS   Raspa,M., Guan,M., Paoletti,R., Montoliu,L., Ayadi,A.,
            Marschall,S., Fray,M. and Scavizzi,F.
  CONSRTM   EMMA/Infrafrontier Technical Working Group
  TITLE     Dry ice is a reliable substrate for the distribution of frozen
            mouse spermatozoa: A multi-centric study
  JOURNAL   Theriogenology 96, 49-57 (2017)
   PUBMED   28532839
REFERENCE   5  (bases 1 to 4148)
  AUTHORS   Golebiewska,E.M., Harper,M.T., Williams,C.M., Savage,J.S.,
            Goggs,R., Fischer von Mollard,G. and Poole,A.W.
  TITLE     Syntaxin 8 regulates platelet dense granule secretion, aggregation,
            and thrombus stability
  JOURNAL   J Biol Chem 290 (3), 1536-1545 (2015)
   PUBMED   25404741
  REMARK    GeneRIF: Syntaxin 8 has a role in regulating platelet dense granule
            secretion, aggregation, and thrombus stability
REFERENCE   6  (bases 1 to 4148)
  AUTHORS   Pirooz,S.D., He,S., Zhang,T., Zhang,X., Zhao,Z., Oh,S.,
            O'Connell,D., Khalilzadeh,P., Amini-Bavil-Olyaee,S., Farzan,M. and
            Liang,C.
  TITLE     UVRAG is required for virus entry through combinatorial interaction
            with the class C-Vps complex and SNAREs
  JOURNAL   Proc Natl Acad Sci U S A 111 (7), 2716-2721 (2014)
   PUBMED   24550300
REFERENCE   7  (bases 1 to 4148)
  AUTHORS   Zhang,L., Kang,L., Bond,W. and Zhang,N.
  TITLE     Interaction between syntaxin 8 and HECTd3, a HECT domain ligase
  JOURNAL   Cell Mol Neurobiol 29 (1), 115-121 (2009)
   PUBMED   18821010
  REMARK    GeneRIF: Syntaxin 8 and HECTd3 have similar subcellular
            localization.
REFERENCE   8  (bases 1 to 4148)
  AUTHORS   Salazar,G., Craige,B., Styers,M.L., Newell-Litwa,K.A.,
            Doucette,M.M., Wainer,B.H., Falcon-Perez,J.M., Dell'Angelica,E.C.,
            Peden,A.A., Werner,E. and Faundez,V.
  TITLE     BLOC-1 complex deficiency alters the targeting of adaptor protein
            complex-3 cargoes
  JOURNAL   Mol Biol Cell 17 (9), 4014-4026 (2006)
   PUBMED   16760431
REFERENCE   9  (bases 1 to 4148)
  AUTHORS   MacLaren,E.J., Bennett,B., Johnson,T.E. and Sikela,J.M.
  TITLE     Expression profiling identifies novel candidate genes for ethanol
            sensitivity QTLs
  JOURNAL   Mamm Genome 17 (2), 147-156 (2006)
   PUBMED   16465594
REFERENCE   10 (bases 1 to 4148)
  AUTHORS   Kim,B.Y., Ueda,M., Kominami,E., Akagawa,K., Kohsaka,S. and
            Akazawa,C.
  TITLE     Identification of mouse Vps16 and biochemical characterization of
            mammalian class C Vps complex
  JOURNAL   Biochem Biophys Res Commun 311 (3), 577-582 (2003)
   PUBMED   14623309
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK003297.1 and BC048167.1.
            
            On Mar 19, 2018 this sequence version replaced NM_018768.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC048167.1, AK003297.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                AK003297.1         2-26
            26-4148             BC048167.1         1-4123
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 41.2 cM"
     gene            1..4148
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /note="syntaxin 8"
                     /db_xref="GeneID:55943"
                     /db_xref="MGI:MGI:1890156"
     exon            1..60
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..754
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /note="isoform 1 is encoded by transcript variant 1;
                     syntaxin-like protein 3I35"
                     /codon_start=1
                     /product="syntaxin-8 isoform 1"
                     /protein_id="NP_061238.1"
                     /db_xref="CCDS:CCDS24863.1"
                     /db_xref="GeneID:55943"
                     /db_xref="MGI:MGI:1890156"
                     /translation="MAPDPWFSTYDSTCQIAQEIAEKIQERNQCERRGEKTPKLTLTI
                     RTLLKNLKVKIDLLKDLLLRAVSTRQITQLEGDRRQNLLDDLVTRERLLLASFKNEGA
                     EPDLIRSSLMSEEAKRGTPNPWLCEEPEETRGLGFDEIRQQQQKIIQEQDAGLDALSS
                     IISRQKQMGQEIGNELDEQNEIIDDLANLVENTDEKLRTEARRVTLVDRKSTSCGMIM
                     VILLLLVAIVVVAVWPTN"
     exon            61..160
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /inference="alignment:Splign:2.1.0"
     exon            161..255
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /inference="alignment:Splign:2.1.0"
     exon            256..366
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /inference="alignment:Splign:2.1.0"
     exon            367..491
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /inference="alignment:Splign:2.1.0"
     exon            492..584
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /inference="alignment:Splign:2.1.0"
     exon            585..686
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /inference="alignment:Splign:2.1.0"
     exon            687..4129
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      839..844
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /note="hexamer: AATAAA"
     polyA_site      861
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /note="major polyA site"
     regulatory      1336..1341
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /note="hexamer: AATAAA"
     polyA_site      1355
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
     regulatory      4034..4039
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /note="hexamer: ATTAAA"
     polyA_site      4054
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
     regulatory      4110..4115
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
                     /note="hexamer: AAGAAA"
     polyA_site      4129
                     /gene="Stx8"
                     /gene_synonym="0610007H08Rik; 1110002H11Rik;
                     4930571E13Rik; CARB"
ORIGIN      
        1 gctacgagca ggaagtgtcc cagctgcggg cggagactgc accatggccc cggacccctg
       61 gttctccaca tacgattcta cgtgtcagat agcccaagaa atcgctgaga agattcaaga
      121 acgaaatcag tgtgaaagaa gaggtgaaaa gacaccgaag cttaccttga caatcagaac
      181 gttgttgaag aatctgaagg taaagatcga ccttttgaag gacttactgc taagagctgt
      241 gtcgacacgc cagataacac aactggaggg ggatcgaaga cagaacctgc tggatgacct
      301 tgtcacccga gagagactgc tcctggcatc gtttaagaat gagggtgcag agccggatct
      361 gatcaggtcc agcctgatga gcgaagaagc aaagcgagga actcccaacc cttggctctg
      421 tgaggagccg gaggagacca gaggtttggg ttttgatgag atccgacaac agcagcagaa
      481 aattattcaa gaacaggacg cgggtcttga tgccctttcc tctatcataa gtcgccaaaa
      541 acaaatgggc caggaaattg ggaacgaact ggatgaacag aatgagatca tcgatgacct
      601 tgccaacctc gttgagaaca cagatgagaa gcttcgaact gaagccaggc gagtgaccct
      661 ggtggacaga aagtcaactt cctgtgggat gatcatggtg attttattgc tgctcgtggc
      721 tattgtggtc gttgcagtgt ggccaaccaa ctgatagcaa taaagggacc acccgctgtg
      781 acacctgcca actacgaatg aaagcccagc atccttttgg tacataacac ctgctctcaa
      841 taaattttcc accgctctgg gctcttggtg taccttgttt gggtagagaa cagagctgag
      901 cagctctgga gaccttccta gagcactggc ccagttgtgt cccctgtctt ctgtcagtga
      961 ttttgttggt agtcagaagt ccctcagatg gccattgtcc cacagctgcc aatggattcc
     1021 agtcaccctc tgtgtgctgg gcatcctgag ggctgtcata ttaataggca gtgtgtgacc
     1081 ttggtggctg cttgtactgc ggaaaaccac gtgcccttgt gcaaatgagt ggctgatcat
     1141 gtgtctgggt gtccagatag caggatctac gtaaggcaag agccacacag aagacatagc
     1201 caacagcctc tctgtcagcc accatgtgta atttcccaca aagcttagat gcccaacagt
     1261 gctggctatt tctgctctaa gagtttctgg accatcttcg tatctggacg tgaacctttt
     1321 caattttgct ttgttaataa acgcgtcctt caagattgat gacacctttc taaaacaaaa
     1381 tccagacgtg gaggcacatg cctttaaccc cagcacttag gagtcagagg cagtggagtt
     1441 caaaaaaaaa aaaacaacaa caacaaaaga catatttgac acctttctaa gtagcccaca
     1501 gccttagatc ttgagggagg gagcagtcag aggaaagcct ggttataaag caggaagtgc
     1561 tgcctccgtc cagcctctcc actggctctg aagccatgtg tgtgccaagt gagggcatgc
     1621 acataagtat ttcagagaca ggactcagag cgaaactgac tgcaaaagat accgggtcta
     1681 cagatgggtg ctggggcagc gagccctctc tcctctctct acagaaagca ggatgggcag
     1741 ttagcctcgc tttctcatag cagaaagaag agttaaagct aatgccagaa gtcttgccgt
     1801 agcattggct tcagtgagga ggagacaggc ctgtggtagg tcctgtgact ttccttaaga
     1861 gaacataatc caaagcctgc gctggacaga atcaggcacc aggaagaaag ccccagtgtc
     1921 acctagactg gtgccctttc ctgggaccct gagaccgagg attccaagtg ttttgtttga
     1981 ctctgactca taccagaatg cagacaggca ggacagcaat ggggtctctg tggcacagca
     2041 aagggtttct gggagctctc aggccagcag tgaagtgtcc atctcgagag cacatcacct
     2101 cagctcgagt tctggctgaa ctacttaccc tggcctccca cgggacaaga cgaagtaaca
     2161 gaagacaaga gagtaagaaa cacgctgccg acgggggttg ccataccact tatcagctct
     2221 gtgaccacag acagacaggt tccctttctg tgccagtggc ctcatgtaca aaaaggtgac
     2281 aatcccccgc tgccccacct tgccccacct cagggctgct gggaggactg ggtgaggtac
     2341 tgaagcacag caagccactt ggctcaggtg gaaggggact cttcctataa cactagtcta
     2401 agccgaggtg tccagcaggc tcaggaccct gtctcccact cttctaagta cacgaactgt
     2461 aggagcaagc caggcagggg caaagtttgc tgctaacttg gccaatgatg cccaagttcc
     2521 tgaggatccc aaggccctag gagctgactt agccagattg tcagggtcat agtataccag
     2581 gaggtggcaa ctggcagagg gtgtgaagca ggctgtgagg ggcggactgg aggtgaagaa
     2641 gagtgtgggc ttgtgtcagg ggcgtgtgat tgggaagaca ggagccttgc tggactgctg
     2701 gccagggctt taagacacag tgtaccctga gacacatttg tcagggcagg gtggttgcta
     2761 atggatcatc caatgtaaga ccaagaatct gtagagctcc atcatccagt cttgtgggaa
     2821 accccaaatc tgtacaaaca gtgtctcctg ggagcccccc actttggggc tcagaagcag
     2881 cagcattgag agtctgttta actttctcac ctcctcttga cttgctcagc acgaagcccc
     2941 ggcaactcgt gggacacttt gaaccagagc cacgaaggcc tggctcagtt gcactgaaag
     3001 cccttttcag gaagacactg gcccatgtct gcacacagtg gcttctgttc ttctggccga
     3061 atgggctcta ggcccagtca gggcattgtg tgaccccgga caagttcctt cccttctgta
     3121 taccatgggc aggggagcgc aggaggaaag ctgcctgaga ggttcatggt gtgaggaccg
     3181 tgggcactgc aggcagggag actcatcccc aggcagtctt cctcaaagat ggctttcccc
     3241 gcaccatcca aagcagccag gcatgcccag ctgagtgggg tgtaccagag agcctgctgc
     3301 ttcaaagccc aggggacagc tcctgtagag tggggtttgc cagcggtgtt tgcaaggcct
     3361 agtttcccag ttactatgtc aggtctgtag accagtgacc tgctgtgagc gttctggaac
     3421 cagtgacctg ctgtgagcat tctggaacca gtgacctgct gtgagcattc tggaaccagt
     3481 gacctgctgt gagcattctg gaaccagtga cctgctgtga gcattctgga accagtgacc
     3541 tgctgtgagc attctggaac cagtgacctg ctgtgagcat tctggaacca gtgacctgct
     3601 gtgagcattc tggaaccagt gacctgctgt gagcattctg gaaccagtga cctgctgtga
     3661 gcattctgga accagtgacc tgctgtgagc attctggaac cagtgacctg ctgtgagcat
     3721 tctggaacca gtgacctgct gtgagcattc tggaaccagt gacctgctgt gagcattctg
     3781 gaaccagtga cctgctgtga gcgttctgga accagtgacc tgctgtgagc attctggaac
     3841 cagtgacctg ctgtgagcgt tctggaacca gtgacctgct gtgagcattc tggaaccagt
     3901 gacctgctgt gagctttctg gaaccagtga cctgctgtga gcgttctaga agtacaccac
     3961 tcacagaaca cagagtggcc aggcagccag agcacccctg tcttcaggga ctttgtcaat
     4021 gaaagtaaac cttattaaaa cagcagcata tgaattgttt aacttaactg attattaatt
     4081 atatgcatct taaatctgga taccatataa agaaatccag atacctggaa aaaaaaaaaa
     4141 aaaaaaaa
//
