LOCUS       NM_019829               2128 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus syntaxin 5A (Stx5a), transcript variant 1, mRNA.
ACCESSION   NM_019829
VERSION     NM_019829.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Norlin,S., Parekh,V. and Edlund,H.
  TITLE     The ATPase activity of Asna1/TRC40 is required for pancreatic
            progenitor cell survival
  JOURNAL   Development 145 (1) (2018)
   PUBMED   29180572
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2128)
  AUTHORS   Rivera-Monroy,J., Musiol,L., Unthan-Fechner,K., Farkas,A.,
            Clancy,A., Coy-Vergara,J., Weill,U., Gockel,S., Lin,S.Y.,
            Corey,D.P., Kohl,T., Strobel,P., Schuldiner,M., Schwappach,B. and
            Vilardi,F.
  TITLE     Mice lacking WRB reveal differential biogenesis requirements of
            tail-anchored proteins in vivo
  JOURNAL   Sci Rep 6, 39464 (2016)
   PUBMED   28000760
  REMARK    GeneRIF: Stx5 is an autophagy target that is inefficiently
            membrane-targeted by alternative pathways; mice without WRB have
            different biogenesis requirements for tail anchored proteins
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2128)
  AUTHORS   Alldred,M.J., Lee,S.H., Petkova,E. and Ginsberg,S.D.
  TITLE     Expression profile analysis of vulnerable CA1 pyramidal neurons in
            young-Middle-Aged Ts65Dn mice
  JOURNAL   J Comp Neurol 523 (1), 61-74 (2015)
   PUBMED   25131634
REFERENCE   5  (bases 1 to 2128)
  AUTHORS   Chen,J., Melton,C., Suh,N., Oh,J.S., Horner,K., Xie,F., Sette,C.,
            Blelloch,R. and Conti,M.
  TITLE     Genome-wide analysis of translation reveals a critical role for
            deleted in azoospermia-like (Dazl) at the oocyte-to-zygote
            transition
  JOURNAL   Genes Dev 25 (7), 755-766 (2011)
   PUBMED   21460039
REFERENCE   6  (bases 1 to 2128)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   7  (bases 1 to 2128)
  AUTHORS   Floyd,J.A., Gold,D.A., Concepcion,D., Poon,T.H., Wang,X.,
            Keithley,E., Chen,D., Ward,E.J., Chinn,S.B., Friedman,R.A.,
            Yu,H.T., Moriwaki,K., Shiroishi,T. and Hamilton,B.A.
  TITLE     A natural allele of Nxf1 suppresses retrovirus insertional
            mutations
  JOURNAL   Nat Genet 35 (3), 221-228 (2003)
   PUBMED   14517553
REFERENCE   8  (bases 1 to 2128)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 2128)
  AUTHORS   Lehtonen,S., Riento,K., Olkkonen,V.M. and Lehtonen,E.
  TITLE     Syntaxin 3 and Munc-18-2 in epithelial cells during kidney
            development
  JOURNAL   Kidney Int 56 (3), 815-826 (1999)
   PUBMED   10469351
REFERENCE   10 (bases 1 to 2128)
  AUTHORS   Hay,J.C., Chao,D.S., Kuo,C.S. and Scheller,R.H.
  TITLE     Protein interactions regulating vesicle transport between the
            endoplasmic reticulum and Golgi apparatus in mammalian cells
  JOURNAL   Cell 89 (1), 149-158 (1997)
   PUBMED   9094723
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC025794.9, CN539678.1, DV649852.1 and BQ030996.1.
            
            On Nov 17, 2009 this sequence version replaced NM_019829.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            CCDS Note: he coding region has been updated to extend the
            N-terminus to one that is supported by available conservation data.
            The use of an alternative upstream start codon would result in a
            protein that is 54 aa longer.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC021883.1, AK166087.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-451               AC025794.9         204574-205024
            452-1107            CN539678.1         117-772
            1108-1680           DV649852.1         72-644
            1681-2128           BQ030996.1         1-448               c
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 5.45 cM"
     gene            1..2128
                     /gene="Stx5a"
                     /gene_synonym="0610031F24Rik; D19Ertd627e; Stx5"
                     /note="syntaxin 5A"
                     /db_xref="GeneID:56389"
                     /db_xref="MGI:MGI:1928483"
     exon            1..695
                     /gene="Stx5a"
                     /gene_synonym="0610031F24Rik; D19Ertd627e; Stx5"
                     /inference="alignment:Splign:2.1.0"
     CDS             471..1538
                     /gene="Stx5a"
                     /gene_synonym="0610031F24Rik; D19Ertd627e; Stx5"
                     /codon_start=1
                     /product="syntaxin-5"
                     /protein_id="NP_062803.4"
                     /db_xref="CCDS:CCDS29542.2"
                     /db_xref="GeneID:56389"
                     /db_xref="MGI:MGI:1928483"
                     /translation="MIPRKRYGSKNTDQGVYLGLSKTQVLSPATAISSSSDSTPLPTP
                     VALVPSPPDTMSCRDRTQEFQSACKSLQSRQNGIQTSKPALHAARQCSEFTLMARRIG
                     KDLSNTFAKLEKLTILAKRKSLFDDKAVEIEELTYIIKQDINSLNKQIAQLQDFVRAK
                     GSQSGRHLQTHSNTIVVSLQSKLASMSNDFKSVLEVRTENLKQQRNRREQFSRAPVSA
                     LPLAPNNLGGGPIILGAESRASRDVAIDMMDPRTSQQLQLIDEQDSYIQSRADTMQNI
                     ESTIVELGSIFQQLAHMVKEQEETIQRIDENVLGAQLDVEAAHSEILKYFQSVTSNRW
                     LMVKIFLILIVFFIIFVVFLA"
     exon            696..766
                     /gene="Stx5a"
                     /gene_synonym="0610031F24Rik; D19Ertd627e; Stx5"
                     /inference="alignment:Splign:2.1.0"
     exon            767..822
                     /gene="Stx5a"
                     /gene_synonym="0610031F24Rik; D19Ertd627e; Stx5"
                     /inference="alignment:Splign:2.1.0"
     exon            823..893
                     /gene="Stx5a"
                     /gene_synonym="0610031F24Rik; D19Ertd627e; Stx5"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1010
                     /gene="Stx5a"
                     /gene_synonym="0610031F24Rik; D19Ertd627e; Stx5"
                     /inference="alignment:Splign:2.1.0"
     exon            1011..1067
                     /gene="Stx5a"
                     /gene_synonym="0610031F24Rik; D19Ertd627e; Stx5"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1149
                     /gene="Stx5a"
                     /gene_synonym="0610031F24Rik; D19Ertd627e; Stx5"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1256
                     /gene="Stx5a"
                     /gene_synonym="0610031F24Rik; D19Ertd627e; Stx5"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1378
                     /gene="Stx5a"
                     /gene_synonym="0610031F24Rik; D19Ertd627e; Stx5"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..2112
                     /gene="Stx5a"
                     /gene_synonym="0610031F24Rik; D19Ertd627e; Stx5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2089..2094
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stx5a"
                     /gene_synonym="0610031F24Rik; D19Ertd627e; Stx5"
                     /note="hexamer: ATTACA"
     polyA_site      2112
                     /gene="Stx5a"
                     /gene_synonym="0610031F24Rik; D19Ertd627e; Stx5"
                     /note="major polyA site"
ORIGIN      
        1 ttcctgtttc aaataaacac ttttaagagg gttttttcgc ctccccctcc gccctccgaa
       61 gtgtgtccac ctctggagat cggttgctgt tttctgtgag agggggaagg aaatcacgta
      121 taccaaaggc aggattgaga cctgggctaa ctgcaaaggt ctgcctggaa gttcagggcc
      181 gcaggaccag gctgtggctg ttttctcgac tttaggggcc ccggccggag ctctggcagt
      241 cagagccaag tcggaaggag actctgagga ggcttcgcct tgggcctgga ggaagctgac
      301 cgttctcgcc gtgtccaggg tgcaggatct gcgggtggcc gtgggctcca aagtctgtca
      361 aatacgggaa agggcctggg gatttgctag ttgaggggag cgtttgggga aagaggctgt
      421 ctggagaatg acattctcct ctccacaaca gtctgaggtt atgcgactcg atgatcccgc
      481 ggaaacgcta cggatctaag aacacggatc agggtgtcta cctgggtctc tcaaagacac
      541 aggttctgtc ccctgcaact gctatcagta gcagcagtga cagcactcct ttgcccactc
      601 cggtggccct ggtcccctcc cctcccgaca ccatgtcctg ccgggatcgg acccaggagt
      661 tccagtctgc ctgcaagtcc ctgcagagcc gtcagaatgg aatccaaaca agcaaaccgg
      721 ctctgcacgc agcccgacag tgcagtgaat ttaccctcat ggccaggcgc attgggaaag
      781 atctcagcaa tacatttgcc aagctggaga agctaacaat cttggcaaag cgcaagtccc
      841 tctttgatga taaagcagta gaaattgagg agctaacata catcatcaaa caggatatca
      901 atagcctcaa caaacaaatt gctcagcttc aagattttgt gagggccaag ggcagccaga
      961 gtggccggca tctgcagacc cattctaaca ccattgtggt ttcgttgcag tcaaagctgg
     1021 cttctatgtc caatgacttc aagtcagttc tagaagtgag gactgagaat ctgaaacagc
     1081 agaggaaccg tcgggaacag ttctccaggg caccagtgtc agccctgcct cttgccccca
     1141 acaaccttgg aggtggtccc ataattttgg gagcagagtc ccgagcttcc agggacgtgg
     1201 ctattgacat gatggaccct agaacaagcc agcagcttca gctcattgat gagcaggatt
     1261 cctacatcca gagtcgggca gacaccatgc agaacattga gtctacaatc gttgagctgg
     1321 gctccatttt tcagcaattg gcacacatgg ttaaagaaca ggaggaaacg attcagagga
     1381 tcgacgagaa tgtgcttgga gcccagctgg atgtggaggc agcccattca gagatcctca
     1441 agtacttcca gtcagttacc tccaatcggt ggctcatggt caaaatcttc ctcatcctca
     1501 ttgtcttctt catcatcttt gtggtcttcc ttgcctgaac cctcctccct cattctgagc
     1561 cactccatgg agggcttggg atcctcctgg gaggacaggt ggctactatt gccactgagc
     1621 ctgtgcaagg tggttgggag aaaggccatt tccttggaac tgctaagaat ggccagtgtc
     1681 cctgattccc cacccttgtc tctggccact ctgtcctatc ctcaggccca tgaaacacac
     1741 tggttctgga tttggactct gctgtgaaga ggctgggaac agaagccagc taggggccag
     1801 tgggggaggt tgtctccacc ttgagatttt tataagccta taaccagcct cccccaaacg
     1861 taactggcag caagaggaga aaacgccctt ccttcttgag aggcaagaat cctcaagaag
     1921 tatccaaggg accaacttca tcatctgggt cagctagaac ttgactgccg ccttctcctc
     1981 accatgtgag gtgagggggc tctgagccct acagttgcct gcacaaacct gactttggct
     2041 actggtgact ctcaatatgc caaacatgct gcagcctatt tcctcccaat tacagcaaga
     2101 ctgtcagcct caaaaaaaaa aaaaaaaa
//
