LOCUS       NM_008408               5810 bp    mRNA    linear   ROD 06-JUN-2024
DEFINITION  Mus musculus STT3, subunit of the oligosaccharyltransferase
            complex, homolog A (S. cerevisiae) (Stt3a), mRNA.
ACCESSION   NM_008408
VERSION     NM_008408.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Wang,D., Wu,S., He,J., Sun,L., Zhu,H., Zhang,Y., Liu,S., Duan,X.,
            Wang,Y. and Xu,T.
  TITLE     FAT4 overexpression promotes antitumor immunity by regulating the
            beta-catenin/STT3/PD-L1 axis in cervical cancer
  JOURNAL   J Exp Clin Cancer Res 42 (1), 222 (2023)
   PUBMED   37658376
  REMARK    GeneRIF: FAT4 overexpression promotes antitumor immunity by
            regulating the beta-catenin/STT3/PD-L1 axis in cervical cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Ramirez,A.S., Kowal,J. and Locher,K.P.
  TITLE     Cryo-electron microscopy structures of human
            oligosaccharyltransferase complexes OST-A and OST-B
  JOURNAL   Science 366 (6471), 1372-1375 (2019)
   PUBMED   31831667
REFERENCE   3  (bases 1 to 5810)
  AUTHORS   Hsu,J.M., Xia,W., Hsu,Y.H., Chan,L.C., Yu,W.H., Cha,J.H.,
            Chen,C.T., Liao,H.W., Kuo,C.W., Khoo,K.H., Hsu,J.L., Li,C.W.,
            Lim,S.O., Chang,S.S., Chen,Y.C., Ren,G.X. and Hung,M.C.
  TITLE     STT3-dependent PD-L1 accumulation on cancer stem cells promotes
            immune evasion
  JOURNAL   Nat Commun 9 (1), 1908 (2018)
   PUBMED   29765039
  REMARK    GeneRIF: N-glycosyltransferases STT3A and STT3B isoforms are
            sufficient and required for effective PD-L1 protein induction in
            the general cell population of mesenchymal-like cancer cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5810)
  AUTHORS   Lara,P., Ojemalm,K., Reithinger,J., Holgado,A., Maojun,Y.,
            Hammed,A., Mattle,D., Kim,H. and Nilsson,I.
  TITLE     Refined topology model of the STT3/Stt3 protein subunit of the
            oligosaccharyltransferase complex
  JOURNAL   J Biol Chem 292 (27), 11349-11360 (2017)
   PUBMED   28512128
  REMARK    GeneRIF: results show that most of the STT3 TMDs are well inserted
            into the ER membrane on their own or in the presence of the natural
            flanking residues.
REFERENCE   5  (bases 1 to 5810)
  AUTHORS   Shibatani,T., David,L.L., McCormack,A.L., Frueh,K. and Skach,W.R.
  TITLE     Proteomic analysis of mammalian oligosaccharyltransferase reveals
            multiple subcomplexes that contain Sec61, TRAP, and two potential
            new subunits
  JOURNAL   Biochemistry 44 (16), 5982-5992 (2005)
   PUBMED   15835887
REFERENCE   6  (bases 1 to 5810)
  AUTHORS   Kelleher,D.J., Karaoglu,D., Mandon,E.C. and Gilmore,R.
  TITLE     Oligosaccharyltransferase isoforms that contain different catalytic
            STT3 subunits have distinct enzymatic properties
  JOURNAL   Mol Cell 12 (1), 101-111 (2003)
   PUBMED   12887896
REFERENCE   7  (bases 1 to 5810)
  AUTHORS   Kim,M.G., Chen,C., Lyu,M.S., Cho,E.G., Park,D., Kozak,C. and
            Schwartz,R.H.
  TITLE     Cloning and chromosomal mapping of a gene isolated from thymic
            stromal cells encoding a new mouse type II membrane serine
            protease, epithin, containing four LDL receptor modules and two CUB
            domains
  JOURNAL   Immunogenetics 49 (5), 420-428 (1999)
   PUBMED   10199918
REFERENCE   8  (bases 1 to 5810)
  AUTHORS   Hunt,C.R., Parsian,A.J., Goswami,P.C. and Kozak,C.A.
  TITLE     Characterization and expression of the mouse Hsc70 gene
  JOURNAL   Biochim Biophys Acta 1444 (3), 315-325 (1999)
   PUBMED   10095055
REFERENCE   9  (bases 1 to 5810)
  AUTHORS   Lissy,N.A., Bellacosa,A., Sonoda,G., Miller,P.D., Jhanwar,S.C. and
            Testa,J.R.
  TITLE     Isolation, characterization, and mapping to human chromosome
            11q24-25 of a cDNA encoding a highly conserved putative
            transmembrane protein, TMC
  JOURNAL   Biochim Biophys Acta 1306 (2-3), 137-141 (1996)
   PUBMED   8634329
REFERENCE   10 (bases 1 to 5810)
  AUTHORS   Hong,G., Deleersnijder,W., Kozak,C.A., Van Marck,E., Tylzanowski,P.
            and Merregaert,J.
  TITLE     Molecular cloning of a highly conserved mouse and human integral
            membrane protein (Itm1) and genetic mapping to mouse chromosome 9
  JOURNAL   Genomics 31 (3), 295-300 (1996)
   PUBMED   8838310
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK150640.1, AK153840.1, AK050050.1 and AC155921.2.
            
            On Jun 19, 2018 this sequence version replaced NM_008408.4.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK168937.1, AK152242.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                AK150640.1         2-45
            45-2741             AK153840.1         2-2698
            2742-3771           AK050050.1         966-1995
            3772-5810           AC155921.2         88750-90788
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 20.67 cM"
     gene            1..5810
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /note="STT3, subunit of the oligosaccharyltransferase
                     complex, homolog A (S. cerevisiae)"
                     /db_xref="GeneID:16430"
                     /db_xref="MGI:MGI:105124"
     exon            1..73
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            74..200
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..2230
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /EC_number="2.4.99.18"
                     /note="integral membrane protein 1; oligosaccharyl
                     transferase subunit STT3A"
                     /codon_start=1
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit STT3A"
                     /protein_id="NP_032434.3"
                     /db_xref="CCDS:CCDS52768.1"
                     /db_xref="GeneID:16430"
                     /db_xref="MGI:MGI:105124"
                     /translation="MTKLGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFAVLRFESV
                     IHEFDPYFNYRTTRFLAEEGFYKFHNWFDDRAWYPLGRIIGGTIYPGLMITSAAIYHV
                     LHFFHITIDIRNVCVFLAPLFSSFTTIVTYHLTKELKDAGAGLLAAAMIAVVPGYISR
                     SVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSIYWAAKCALAYFYMVSSWGGYVFLINL
                     IPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGL
                     CQIHAFVDYLRSKLNPQQFEVLFRSVISLVGFVLLTVGALLMLTGKISPWTGRFYSLL
                     DPSYAKNNIPIIASVSEHQPTTWSSYYFDLQLLVFMFPVGLYYCFSNLSDARIFIIMY
                     GVTSMYFSAVMVRLMLVLAPVMCILSGIGVSQVLSTYMKNLDISRPDKKSKKQQDSTY
                     PIKNEVASGMILVMAFFLITYTFHSTWVTSEAYSSPSIVLSARGGDGSRIIFDDFREA
                     YYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKA
                     YEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTETGRHIKENDYYTPTGEF
                     RVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAY
                     TTEHWLVRIYKVKDLDNRGLSRT"
     exon            201..261
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            262..383
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            384..529
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            530..620
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            621..727
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            728..892
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1073
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1229
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..1321
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1477
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..1666
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1783
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1784..1886
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1887..2075
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2076..2191
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2192..5810
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2723..2728
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /note="hexamer: AATAAA"
     polyA_site      2724
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /note="major polyA site"
     regulatory      3874..3879
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /note="hexamer: AATAAA"
     polyA_site      3895
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
     regulatory      5172..5177
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /note="hexamer: ATTAAA"
     polyA_site      5196
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
     regulatory      5790..5795
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
                     /note="hexamer: ATTAAA"
     polyA_site      5810
                     /gene="Stt3a"
                     /gene_synonym="B5; Itm1"
ORIGIN      
        1 gctgtcaggg ttgagtgcgc cgctgaacgg atggcagggg gagcagagtg ggttcctgag
       61 gagcatccgt gaggtatttg aatatcatca gttgccaccc attgatgtca agatgactaa
      121 gcttggattt ttgcgattgt cctatgagaa gcaggacaca cttctaaagc ttctcatcct
      181 gtcgatggct gctgtgttat ctttttctac tcgtcttttt gctgtgctga gatttgaaag
      241 tgtcatccat gagtttgatc cgtactttaa ttatcggact acccggtttc tggctgagga
      301 ggggttttat aaattccata actggtttga tgaccgggct tggtaccctt tgggccgaat
      361 cattggagga acaatttacc caggtttaat gatcacttct gctgcaatct accatgtact
      421 ccatttcttc catatcacca ttgacattcg gaatgtctgt gttttcctgg ccccgctttt
      481 ctcctctttc accaccatcg ttacgtacca ccttaccaaa gagctcaagg atgcaggagc
      541 tgggcttctt gctgctgcca tgattgctgt agttcctggg tatatttctc gatctgtagc
      601 tggctcctat gataatgaag gaattgctat cttttgcatg ctgcttactt actacatgtg
      661 gatcaaggca gtgaagactg gttccatcta ttgggctgcc aagtgtgccc tcgcttattt
      721 ctacatggtc tcttcatggg gaggctatgt gttcctgatc aacttgattc ctctacatgt
      781 cctggtgcta atgctgacag gccgtttttc tcaccggatc tacgtagcct actgtactgt
      841 ttactgcctg ggcaccattc tttctatgca gatttccttt gttggtttcc agcccgtcct
      901 ttcatcagaa cacatggcag cctttggagt gtttggtctc tgtcagatcc atgctttcgt
      961 agattacctg cgcagcaagt tgaatccaca gcaattcgaa gttcttttcc ggagtgttat
     1021 ctccctggtt ggctttgtcc tcctcactgt gggagctctc ctcatgctaa caggaaaaat
     1081 ttctccctgg acagggcgtt tctactctct gctggatccc tcttatgcta agaataacat
     1141 tcccattatt gcatctgttt ctgagcacca gcccacaacc tggtcttcct actattttga
     1201 tctacagctc cttgtcttca tgtttccagt tggcctctat tactgcttta gcaacctgtc
     1261 tgatgctcgg atttttatca tcatgtatgg tgtgaccagc atgtactttt cagctgtaat
     1321 ggtgcgtcta atgctggtat tggcacctgt tatgtgcatt ctttctggca ttggtgtttc
     1381 ccaggtgctg tccacatata tgaaaaatct ggacataagt cgcccagaca agaagagcaa
     1441 gaagcaacag gattctactt accctattaa gaatgaggtg gcgagtggga tgatactggt
     1501 catggctttc tttctcatca cctacacgtt tcattcgact tgggtgacca gtgaagccta
     1561 ttcttctccc tccattgttc tgtctgctcg tggtggggat ggcagtagga tcatttttga
     1621 tgacttccga gaagcgtatt attggctccg tcacaatact ccagaggatg caaaagtcat
     1681 gtcatggtgg gattatggct accaaattac tgcaatggca aatcggacaa ttttagtgga
     1741 caataacaca tggaataata cccatatttc tcgagtaggg caggcaatgg catccacaga
     1801 agaaaaagcc tatgaaatca tgagggagct tgatgtcagc tatgtgcttg tcatttttgg
     1861 aggccttact gggtattctt cggatgatat caacaagttt ctttggatgg tccggattgg
     1921 aggaagcaca gagacaggaa gacacattaa ggagaatgac tactatactc ctactgggga
     1981 attccgtgtt gatcgtgagg gttctccggt gctgctcaac tgccttatgt acaaaatgtg
     2041 ttactaccgc tttgggcagg tctacacaga agccaagcgt ccaccaggct ttgaccgtgt
     2101 tcgaaatgct gagattggta ataaagactt tgagcttgat gtcctggagg aagcgtatac
     2161 cacagaacac tggctagtca ggatatacaa ggtaaaggac ctggataatc gaggcttgtc
     2221 aaggacataa acgtcacatt gtgccctgag cattatgctt cgcactgagc gcgtcatgtt
     2281 gaggacgctg aagatgtttt ttatatgcag tttataagaa cagccggatg gggttagaat
     2341 tgtctgcaag ttttgccctg gacaatatgg gctgggccaa gtgaaatgat ttttataatt
     2401 ctgagcaggt taccaaatga aatgttatgg ctttactttg gtcaattaaa aggggggggg
     2461 gatttttttt aaatgtgcct tatttgtttt gacttaaatt ggctgatacg aggatcacag
     2521 aagtgagcgg atggaagacc atatccatgc tctaggtccc caaatgaacc agataggagc
     2581 atttttttct cctatcagca atctcaagga ctagctctgg ttcaacaaat gtaaacaaca
     2641 actttgtcac acttttttgt tttttagcac ccaggtacaa tgctttcctt ataatgggtg
     2701 cttaataaat ttttatcaaa tgaataaatg tttctgggac cagaggagtg ctgtttctgg
     2761 gcaagaaaga cagctttctt gctgttatgt ctatgttctc gatgtctatt tctttagaag
     2821 ctctttggct ttataaggac agaaagttgc tgagtattcc tgatctcacc agtatccttt
     2881 caaactaatg gcagttattc tttttctaag tagaaatgtg aagcaaaagt gactaatcca
     2941 gtagttctta agatcagtga aacatcaatc ctagaggaag acactcctcc aacatcaggt
     3001 tgatgatcag tagatgtttc tggaatcaga tgtcattatg tggacctaca tgaagtttag
     3061 gcattcaata cttcactaaa cctaaaacat agtaaatggc tctttgctca ttttctctag
     3121 actgcactaa atctcttagg gttatccacc gtgagcttct gtttttctta atgtcagtct
     3181 gctgtgttca tataccactt tgaggcttga ttacttggtt ttgaatccct tgctaaatat
     3241 atacatagga agtgggaagt attgctacta gcaggtactt tgatatcatt gccagtctga
     3301 ctgcaagaaa agagacttca aaaactacaa agcacagaga tcccatagat attttgtgtc
     3361 aggataaata gctttctgtt ctgtataata ggatatttca tgtaatttac aacatagggc
     3421 ataatcactg atgtatataa accatcttaa attaattata gtgtttctat ttaagaaaac
     3481 aggaggaatt atatgacttt tagatagtca cagtggtact caatgcatat gtgcttaggt
     3541 gtacttgtat acctaagggc ttttccttca aaccggtgta tgtactgtgt attttaactg
     3601 tgggtgaaat tttagattta acagatgatt tgatatccta tatgtaaata cattatttga
     3661 tttatataca gaactactgc ctagtgggaa aggttaacct gagacttatc aatatgatta
     3721 acattacagg ttattagtaa gctttctcta tagtataaat gggatacccc cttttcatat
     3781 cattttgact cctgggatct tgatattttc tagtctacaa agatttttaa gttgatttca
     3841 ttttcagttg tagtcttctt agtgtaaaaa aaaaataaat ccacctcaaa agcaacaata
     3901 aaatttcaca cacatgccca cccacacacc cagccagcac tcgccagaga ctcttgcttc
     3961 caggtctggt gaacctcgca ctgtgcctcc tgcctgatca ttagtaactt gctttgttat
     4021 gttttggtgt tttagtttga ggtaacattg atttcttttt tctttctttc tttttttttt
     4081 tttttttttt aaccagtggc tttcaacatt gtgcgtcatg ttctctttgg caaaccatta
     4141 tctcccaaag tatttacatt gcaattcata acagcaaaat cttagctatg aggtcacagt
     4201 gaaaatactt ttatggctgg gatgtcacca caagatgatg agctgtatta aagagtagca
     4261 gccttaggaa ggttgagaac cactgcccta tgttttgtga caccattttt actatcatct
     4321 gttactgttc taattacttt tttttttttt ttaaagactg ttccatgtag ctcaggccag
     4381 ctttgaactc aagatacaga tgatgctagc tttgaactct tgatcttcct gcctctacct
     4441 cccagttgtg ggctactaca gctagttagg tactattttt cctatatatt ttaaaggaaa
     4501 tactttaatt ttttggtact tgttatcttt cctgcttcct taccacccca gctagttctc
     4561 cctcatttgt gcccattttc tcatactgtg atcaaaccta ttcgttaaac aactttgggt
     4621 gttttgagac agtatctttt atagctaggg ctggctttga attcctgatt ctcctctctc
     4681 tatttgccaa atgctggtat taagatgtat gccaccatac ttagttacat tgggtttttc
     4741 agaatgtctt tgatttttca gcttgaaata actcttcagg tttgttgaat tatattattt
     4801 tactgttgta tttgttgatc tttgccattt tcattgattt gccatgtctt agtatcttct
     4861 tatatgtagg ggaaatacat ttctggagct cttctctatc atactcaatg catagctggg
     4921 tatagcattc cagactggcg ctctttacat tataaaggca ctggcccact gttctaatgt
     4981 tgagaaatgg cagttatttt gatgagtatt tgtctattct ttgccccaga tcttttttgc
     5041 atcttttttg tgggtgttca ctgtcctggg tactctggat tttcatgtgt gcatagtcaa
     5101 atttttctgt tttttttttt ttttttttca aacagttata tatggattcc tccaattctc
     5161 tgataaggct aattaaaacg ttcatatttt aggcaacatg gtatgacact tgaaatattt
     5221 aaatttttgt gtttgggtgt tttaccacat atatgtgtat catgtgcatt cctagtaccc
     5281 atagaatcca gaggaagaca tcagattcct ggcactggag ttatcgacag ttgtgagttg
     5341 ccatgcaagt gctcggaatt gaaccctttg taagaacagc cattggtttt aactgttgaa
     5401 ttaactcccc agtccatgat gaagttattt gagtgtgggg ctttctgagt cagttttcta
     5461 atagtttctt agttgctctt ctttattcta cctttttgga gatttcctca gctgtatttg
     5521 tggtcttgct ctgttttccc ctccctccct ccctccctct ctttttcctc ccttcctctt
     5581 ccctccctct ccccttttgt atgttgtgcg taggtttaag cccagggccc tgtgcatgct
     5641 aggtaagcac tcttaacagt gagcaacatc tcttaatttc aaccatattt aaaaaaaata
     5701 acttttttct tttctgcttt catatttcta aagtcttaag cactcttgtt tagagatctc
     5761 ttttgtccca ttgttttgct gcctttccaa ttaaagactt ttccaaattc
//
