LOCUS       NM_001252448            2578 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus STE20-related kinase adaptor alpha (Strada),
            transcript variant 1, mRNA.
ACCESSION   NM_001252448
VERSION     NM_001252448.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Dang,L.T., Glanowska,K.M., Iffland Ii,P.H., Barnes,A.E., Baybis,M.,
            Liu,Y., Patino,G., Vaid,S., Streicher,A.M., Parker,W.E., Kim,S.,
            Moon,U.Y., Henry,F.E., Murphy,G.G., Sutton,M., Parent,J.M. and
            Crino,P.B.
  TITLE     Multimodal Analysis of STRADA Function in Brain Development
  JOURNAL   Front Cell Neurosci 14, 122 (2020)
   PUBMED   32457579
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2578)
  AUTHORS   Veleva-Rotse,B.O., Smart,J.L., Baas,A.F., Edmonds,B., Zhao,Z.M.,
            Brown,A., Klug,L.R., Hansen,K., Reilly,G., Gardner,A.P.,
            Subbiah,K., Gaucher,E.A., Clevers,H. and Barnes,A.P.
  TITLE     STRAD pseudokinases regulate axogenesis and LKB1 stability
  JOURNAL   Neural Dev 9, 5 (2014)
   PUBMED   24594058
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2578)
  AUTHORS   Parker,W.E., Orlova,K.A., Parker,W.H., Birnbaum,J.F.,
            Krymskaya,V.P., Goncharov,D.A., Baybis,M., Helfferich,J.,
            Okochi,K., Strauss,K.A. and Crino,P.B.
  TITLE     Rapamycin prevents seizures after depletion of STRADA in a rare
            neurodevelopmental disorder
  JOURNAL   Sci Transl Med 5 (182), 182ra53 (2013)
   PUBMED   23616120
REFERENCE   5  (bases 1 to 2578)
  AUTHORS   Smith,C.D., Compton,R.A., Bowler,J.S., Kemp,J.T., Sudweeks,S.N.,
            Thomson,D.M. and Winder,W.W.
  TITLE     Characterization of the liver kinase B1-mouse protein-25
            -Ste-20-related adaptor protein complex in adult mouse skeletal
            muscle
  JOURNAL   J Appl Physiol (1985) 111 (6), 1622-1628 (2011)
   PUBMED   21903876
  REMARK    GeneRIF: Quantitative PCR revealed significantly reduced LKB1,
            MO25alpha, and STRADbeta mRNA in LKB1(-/-) muscle. These findings
            demonstrate that the LKB1-MO25-STRAD complex is the principal AMPKK
            in skeletal muscle.
REFERENCE   6  (bases 1 to 2578)
  AUTHORS   Matsuki,T., Matthews,R.T., Cooper,J.A., van der Brug,M.P.,
            Cookson,M.R., Hardy,J.A., Olson,E.C. and Howell,B.W.
  TITLE     Reelin and stk25 have opposing roles in neuronal polarization and
            dendritic Golgi deployment
  JOURNAL   Cell 143 (5), 826-836 (2010)
   PUBMED   21111240
REFERENCE   7  (bases 1 to 2578)
  AUTHORS   Orlova,K.A., Parker,W.E., Heuer,G.G., Tsai,V., Yoon,J., Baybis,M.,
            Fenning,R.S., Strauss,K. and Crino,P.B.
  TITLE     STRADalpha deficiency results in aberrant mTORC1 signaling during
            corticogenesis in humans and mice
  JOURNAL   J Clin Invest 120 (5), 1591-1602 (2010)
   PUBMED   20424326
  REMARK    GeneRIF: aberrant nuclear accumulation of LKB1 caused by STRADalpha
            deficiency contributes to hyperactivation of mTORC1 signaling and
            disruption of neuronal lamination during corticogenesis
REFERENCE   8  (bases 1 to 2578)
  AUTHORS   Denison,F.C., Hiscock,N.J., Carling,D. and Woods,A.
  TITLE     Characterization of an alternative splice variant of LKB1
  JOURNAL   J Biol Chem 284 (1), 67-76 (2009)
   PUBMED   18854309
REFERENCE   9  (bases 1 to 2578)
  AUTHORS   Barnes,A.P., Lilley,B.N., Pan,Y.A., Plummer,L.J., Powell,A.W.,
            Raines,A.N., Sanes,J.R. and Polleux,F.
  TITLE     LKB1 and SAD kinases define a pathway required for the polarization
            of cortical neurons
  JOURNAL   Cell 129 (3), 549-563 (2007)
   PUBMED   17482548
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL596331.15.
            
            On Dec 21, 2022 this sequence version replaced NM_001252448.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK138358.1, SRR17784651.636261.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-112               AL596331.15        201906-202017       c
            113-192             AL596331.15        195541-195620       c
            193-250             AL596331.15        193002-193059       c
            251-279             AL596331.15        189395-189423       c
            280-382             AL596331.15        182119-182221       c
            383-504             AL596331.15        181693-181814       c
            505-613             AL596331.15        179596-179704       c
            614-737             AL596331.15        179394-179517       c
            738-909             AL596331.15        176749-176920       c
            910-1014            AL596331.15        176252-176356       c
            1015-1256           AL596331.15        173160-173401       c
            1257-1299           AL596331.15        172871-172913       c
            1300-2578           AL596331.15        171353-172631       c
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 68.89 cM"
     gene            1..2578
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /note="STE20-related kinase adaptor alpha"
                     /db_xref="GeneID:72149"
                     /db_xref="MGI:MGI:1919399"
     exon            1..112
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            113..192
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..1452
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein kinase LYK5; STE20-related kinase adapter protein
                     alpha; STRAD alpha; STE20-related adapter protein"
                     /codon_start=1
                     /product="STE20-related kinase adapter protein alpha
                     isoform 1"
                     /protein_id="NP_001239377.1"
                     /db_xref="CCDS:CCDS56816.1"
                     /db_xref="GeneID:72149"
                     /db_xref="MGI:MGI:1919399"
                     /translation="MSFLVSKPERIRRWVSEKFIVEGLRDLELFGEQPPGDTRRKANE
                     ASSESIASFSKPEMMSSFLPEGGCYELLTIIGKGFEDLMTVNLARYKPTGEYVTVRRI
                     NLEACSNEMVTFLQGELHVSKLFSHPNIVPYRATFIADNELWVVTSFMAYGSAKDLIG
                     THFMDGMNELAIAYILQGVLKALDYIHHMGYVHRSVKASHILISTDGKVYLSGLRSNL
                     SMISHGQRQRAVHDFPKYSIKVLPWLSPEVLQQNLQGYDAKSDIYSVGITACELANGH
                     VPFKDMPATQMLLEKLNGTVPCLLDTSTIPAEELTMSPSRSIANPGLNDSLAAGSLRP
                     SNGDSPSHPYHRTFSPHFHNFVEQCLQRNPDARPNASTLLNHSFFKQIKRRASEALPE
                     LLRPVTPITNFEGSQSQDHSGIFGLVTNLEDLEVDDWEF"
     exon            193..250
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            251..279
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            280..382
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            383..504
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            505..613
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            614..737
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            738..909
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1014
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1256
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1299
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..2578
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2144..2149
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2161
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /note="major polyA site"
     regulatory      2559..2564
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
                     /note="hexamer: TATAAA"
     polyA_site      2578
                     /gene="Strada"
                     /gene_synonym="2610019A05Rik; 6030402H20Rik;
                     E130112C09Rik"
ORIGIN      
        1 atgtcacgtt gcgtacctcc ctcccagcaa ccggcctggc ggcagcgcgg ctacaaaact
       61 gaggaggcgg agccgagacg gagtcggtac tgcgctctga ctcctagacc aggtttaagt
      121 ttttgaagtt gaagtaggtc tacacagtag gaacccatgt cttttcttgt aagtaaacca
      181 gagcgcatta ggcggtgggt ctcggaaaag ttcattgttg agggcttaag agatttggaa
      241 ctgtttggag agcagcctcc gggtgacact cggagaaaag ccaatgaggc gagctcagag
      301 tccatagcat ctttctccaa accagagatg atgagcagct ttcttccaga gggcgggtgt
      361 tatgagctgc tcaccatcat aggcaaaggg tttgaggact tgatgacagt gaatttagcc
      421 aggtacaaac caacgggaga gtatgtgaca gtacgaagga tcaacctaga agcttgttcc
      481 aatgagatgg tgacattctt gcagggggag cttcatgtct ctaaactctt cagccatccc
      541 aatatagtac cgtatcgagc caccttcatc gcagataatg agctgtgggt cgtcacatca
      601 tttatggcgt acggctctgc aaaggatctc attggcacac acttcatgga tggcatgaat
      661 gaactggcga ttgcatacat cctgcagggg gtgctaaagg ccttagacta catccatcac
      721 atgggctatg tgcacaggag tgtcaaggcc agccacattc tgatctccac ggatgggaag
      781 gtctacctgt ctggtttacg cagcaacctt agcatgatta gtcacgggca gcggcagcgt
      841 gcagtccacg atttccccaa atatagtatc aaggtcctgc cttggctcag cccagaagtc
      901 ctccagcaga atcttcaggg ttacgatgcc aagtccgata tctacagtgt gggaatcaca
      961 gcatgtgaac tagccaatgg ccatgtcccc ttcaaagata tgcctgccac tcagatgctg
     1021 ctagaaaaac ttaatggcac agtgccctgc ctgctggaca ccagcaccat ccctgccgag
     1081 gagctgacca tgagtccctc ccgctccatc gccaaccctg gcctgaatga cagcctggct
     1141 gctggtagcc tccggccttc caatggcgac tcgccttccc acccctacca ccgaaccttc
     1201 tcgcctcact tccacaactt tgtggaacag tgccttcagc gcaaccctga cgcaaggcca
     1261 aacgccagca ccctgctgaa ccactccttc ttcaagcaga tcaagcggcg tgcttccgag
     1321 gctttgcctg agttgctccg cccagtcact cccatcacca acttcgaggg cagccagtct
     1381 caggatcaca gtggaatctt cggcctggtg acaaacctag aagacctgga ggtggatgac
     1441 tgggagttct gagtctctgc agcctgtcct ccagctggct ggcgtggggt atgagcctgg
     1501 ggccttcttg aggaccagca tctgcctctg gccacagact gggttcaaag gatattctgc
     1561 ctgccaagct ggctgttggc tacttgaaag gacattctag aagggacttc actgtttttg
     1621 tggcttttga gacccaggga atcccaatca ccagggactt ggagaggccg gaaagctgac
     1681 atcctgttgt gtgagctcag gggacctcct tagaaggaag aagtgctgca tgggccccga
     1741 ggctcacggc caggcccaga gtaactcttg acccagggac cttcacatcc tcttcccaac
     1801 acttgctctc agtagcctag tccctcttcc ctctgttccc atgtcctgaa gggctgaaag
     1861 gggacaagtc ctaagctgtc tggccctgcc cagccgcagg cattgcccca gctactgagc
     1921 accaggtcag ttaaggctgg gactggccat cctcatctcc acctctcgtg ggccctgaca
     1981 cctgaaacca tcttagctca tttattacac tcctttccca agaatccatc ttactgcctc
     2041 ctgccagctg ctgccttggt gcctcctcca agggccatac atgtcttgcc tcatctgagg
     2101 gcttaagagt ccttaagttg tattagtttc attgtcagaa caaattaaaa ttttcagaga
     2161 atgttgggaa tggcaccagt gtttccttgt gttcagagcc tgtatgctgg cttctgggat
     2221 tgccaggctg gattggctgc aggtcactgg ttcttccaga gtgggctatg tggacttgtg
     2281 ccgctggcct ggcagtgatt tctttcataa gcatacatac cagtttctta gaaaactggt
     2341 cttagaagaa acatccgtgg tcaccttttt cttttctttt cttttctttt cttttccgag
     2401 acagggtttc tctgtgtagt cctggttatc ctggaactct gtagaccagg ctggcttcga
     2461 actcagaaat ccgcctgcct ctgcctccct agtgctggga ttaaaggcat gcgccaccac
     2521 gcccggctcc gtggtctctt aattccctag agtttgagta taaaattttt ttaaaaga
//
