LOCUS       NM_016866               3303 bp    mRNA    linear   ROD 31-DEC-2024
DEFINITION  Mus musculus serine/threonine kinase 39 (Stk39), transcript variant
            1, mRNA.
ACCESSION   NM_016866
VERSION     NM_016866.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Wu,A., Zhang,Y., Bock,F., Arroyo,J.P., Delpire,E., Zhang,M.Z.,
            Harris,R.C. and Terker,A.S.
  TITLE     Macrophage SPAK deletion limits a low potassium-induced kidney
            inflammatory program
  JOURNAL   Am J Physiol Renal Physiol 327 (5), F899-F909 (2024)
   PUBMED   39298551
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Xu,Q., Liu,F., Wu,Z., Chen,M., Zhou,Y. and Shi,Y.
  TITLE     Suppression of STK39 weakens the MASLD/MASH process by protecting
            the intestinal barrier
  JOURNAL   Biosci Trends 18 (3), 289-302 (2024)
   PUBMED   38925962
  REMARK    GeneRIF: Suppression of STK39 weakens the MASLD/MASH process by
            protecting the intestinal barrier.
REFERENCE   3  (bases 1 to 3303)
  AUTHORS   Welling,P.A., Little,R., Al-Qusairi,L., Delpire,E., Ellison,D.H.,
            Fenton,R.A. and Grimm,P.R.
  TITLE     Potassium-Switch Signaling Pathway Dictates Acute Blood Pressure
            Response to Dietary Potassium
  JOURNAL   Hypertension 81 (5), 1044-1054 (2024)
   PUBMED   38465625
REFERENCE   4  (bases 1 to 3303)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 3303)
  AUTHORS   de Boer,L.L., Vanes,L., Melgrati,S., Biggs O'May,J., Hayward,D.,
            Driscoll,P.C., Day,J., Griffiths,A., Magueta,R., Morrell,A.,
            MacRae,J.I., Kochl,R. and Tybulewicz,V.L.J.
  TITLE     T cell migration requires ion and water influx to regulate actin
            polymerization
  JOURNAL   Nat Commun 14 (1), 7844 (2023)
   PUBMED   38057317
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3303)
  AUTHORS   Gagnon,K.B., England,R. and Delpire,E.
  TITLE     Volume sensitivity of cation-Cl- cotransporters is modulated by the
            interaction of two kinases: Ste20-related proline-alanine-rich
            kinase and WNK4
  JOURNAL   Am J Physiol Cell Physiol 290 (1), C134-C142 (2006)
   PUBMED   15930150
  REMARK    GeneRIF: WNK4 interacting with SPAK, which in turn phosphorylates
            and activates NKCC1 and phosphorylates and deactivates KCC2
REFERENCE   7  (bases 1 to 3303)
  AUTHORS   Chen,B., Schaevitz,L.R. and McConnell,S.K.
  TITLE     Fezl regulates the differentiation and axon targeting of layer 5
            subcortical projection neurons in cerebral cortex
  JOURNAL   Proc Natl Acad Sci U S A 102 (47), 17184-17189 (2005)
   PUBMED   16284245
REFERENCE   8  (bases 1 to 3303)
  AUTHORS   Piechotta,K., Garbarini,N., England,R. and Delpire,E.
  TITLE     Characterization of the interaction of the stress kinase SPAK with
            the Na+-K+-2Cl- cotransporter in the nervous system: evidence for a
            scaffolding role of the kinase
  JOURNAL   J Biol Chem 278 (52), 52848-52856 (2003)
   PUBMED   14563843
  REMARK    GeneRIF: SPAK interacts with the cotransporter and serves as an
            intermediate in the response to cellular stress
REFERENCE   9  (bases 1 to 3303)
  AUTHORS   Piechotta,K., Lu,J. and Delpire,E.
  TITLE     Cation chloride cotransporters interact with the stress-related
            kinases Ste20-related proline-alanine-rich kinase (SPAK) and
            oxidative stress response 1 (OSR1)
  JOURNAL   J Biol Chem 277 (52), 50812-50819 (2002)
   PUBMED   12386165
  REMARK    GeneRIF: Cation chloride cotransporters interact with this protein
REFERENCE   10 (bases 1 to 3303)
  AUTHORS   Johnston,A.M., Naselli,G., Gonez,L.J., Martin,R.M., Harrison,L.C.
            and DeAizpurua,H.J.
  TITLE     SPAK, a STE20/SPS1-related kinase that activates the p38 pathway
  JOURNAL   Oncogene 19 (37), 4290-4297 (2000)
   PUBMED   10980603
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844841.10, AL845486.6 and BX936296.17.
            
            On Oct 4, 2022 this sequence version replaced NM_016866.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC064443.1, AF099988.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164141
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-404               AL844841.10        28404-28807         c
            405-517             AL845486.6         163280-163392       c
            518-626             AL845486.6         151675-151783       c
            627-768             AL845486.6         145362-145503       c
            769-824             AL845486.6         144165-144220       c
            825-934             AL845486.6         122247-122356       c
            935-1036            AL845486.6         121648-121749       c
            1037-1170           AL845486.6         119325-119458       c
            1171-1219           AL845486.6         112237-112285       c
            1220-1285           AL845486.6         112065-112130       c
            1286-1408           AL845486.6         67732-67854         c
            1409-1438           AL845486.6         67615-67644         c
            1439-1501           AL845486.6         62243-62305         c
            1502-1569           AL845486.6         60292-60359         c
            1570-1622           AL845486.6         20290-20342         c
            1623-1691           AL845486.6         16486-16554         c
            1692-1756           BX936296.17        160428-160492       c
            1757-3303           BX936296.17        149971-151517       c
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2"
     gene            1..3303
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /note="serine/threonine kinase 39"
                     /db_xref="GeneID:53416"
                     /db_xref="MGI:MGI:1858416"
     exon            1..404
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            405..517
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            518..626
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            627..768
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            769..824
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            825..934
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1036
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1170
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1219
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1285
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1408
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..1438
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1501
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1569
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1622
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..1691
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1756
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1757..3303
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3282..3287
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /note="hexamer: AATAAA"
     polyA_site      3303
                     /gene="Stk39"
                     /gene_synonym="DCHT; Gm50618; RF005; Rnl5; SPAK"
                     /note="major polyA site"
ORIGIN      
        1 tggctccctc ccctcgtggc aggctccggc ggcggcggcg gctgcggcgg acggggaggg
       61 cgtgcgccgg ccgagaggtg tgggcgacga ggctagggaa gtttcaagtg gaaggtcgtc
      121 cgccggccgg cgcgtcccct cactctcctc ccgcagcatc atggcggagc cgagcggctc
      181 gcctgtgcac gtccagcttt cccagcaggc ggccccggtg acagcggcgg cggcgacggc
      241 cccggcggcc gcgacatcag caccagcccc ggccccggcc ccggctccgg cggcgtccgc
      301 agccccggcc ccggctccag ctgctgctcc agccccggcc ccggcagctc aggcggtcgg
      361 ctggcccatc tgcagggacg cgtacgagct ccaggaggtt atcggcagcg gagcaactgc
      421 ggtggttcag gcagccctat gcaaacccag gcaagaacgc gtagccataa agcggatcaa
      481 cttggaaaaa tgccagacga gtatggatga actcctgaaa gaaattcaag ccatgagcca
      541 atgcagccat cccaacgtag tgacttatta cacctccttt gtggtcaaag atgagctgtg
      601 gctggtcatg aaattactaa gtggaggttc catgttggat atcatcaaat acatcgttaa
      661 tcgaggagaa cataaaaatg gtgtcctaga agaggcgata attgcaacaa ttcttaagga
      721 ggttttggaa ggtttagact atctgcacag aaacggtcag atccataggg atttgaaagc
      781 tggaaatatt cttctgggtg aggatggctc agtacaaata gcagattttg gagtaagcgc
      841 attcttagcc acagggggtg atgttacacg gaataaagtc agaaaaacat ttgttggtac
      901 cccatgttgg atggcccctg aagtcatgga acaggtgaga ggctatgact tcaaggctga
      961 tatgtggagt tttggaataa ctgccattga attagcaacc ggagcagcgc cttaccacaa
     1021 ataccctcca atgaaagtgc taatgctgac tttgcaaaat gacccgccca ctttagaaac
     1081 tggtgtagag gataaagaaa tgatgaaaaa atacggcaag tccttcagaa agttactgtc
     1141 actgtgtctt cagaaagatc cttccaaaag gcccacagca gcagaacttt taaaatgcaa
     1201 gttcttccag aaagccaaga acagagagta cctgatcgag aagctgctga caagaacccc
     1261 agacatagcc caaagagcca agaaggtaag gcgagttcct gggtcgagcg gtcaccttca
     1321 taagactgaa gacggcgact gggagtggag cgatgacgag atggatgaga agagcgagga
     1381 ggggaaggca gccgcctccc aagagaagtc acgaagagta aaagaagaga actcagagat
     1441 ctcggtgaat gctggtggca tccctgagca aatacagtct ctctctgtgc acgactctca
     1501 ggcccaacca aacgctaatg aagactacag agaaggtcct tgtgcagtca accttgtttt
     1561 aagattaaga aactccagaa aggaacttaa tgacatacga tttgagttta ctccaggaag
     1621 agatacagca gacggtgtgt ctcaggagct cttctctgct ggcttggttg acggccatga
     1681 tgtagttata gtggctgcta atttacagaa gattgtagat gaccccaaag ctttaaaaac
     1741 gttgacattt aagttggctt ctggctgcga tgggtcggag attcctgatg aagtgaagct
     1801 gatcgggttc gcccagttga gtgtgagctg atgtacgccc cttgctgtca ccctcatctg
     1861 tcgtgaccct tccctctccc agctccccct ttgcctcctt cctctcaccc cctcactccc
     1921 aaatcccaac aaataagaag attgtgaaga ggctggcttc gatgaagtgc gatcaaaaaa
     1981 aaataataat aataatttaa aaaaaaaaaa ctgtgagcac tccgagttct gctttattgt
     2041 ccagcaaccc acagtgtgag cacaggcaag ccctacagct gcgcaactgt tgctgattcc
     2101 tttccagaga gctatttact attcttagca gtcaatttgg ctagcccgta agtgaggcgt
     2161 cggaaacaca ctcaggtggt cctatctctt ggcagcaaaa ggaatcttct accagaagcc
     2221 ttttctcctc ttactgttgt atttctgttt aagactttaa ttgtacatct gacaatactg
     2281 cctcttttat gttgtactta gaaattaata tacttataaa attaagattt attagccaaa
     2341 cttgaattct agttttaaaa ctgactgtga attttatttt tcatatattt atgctttaca
     2401 caccgtagct ccgaggggga aagttttgat tacgcgcttt ctgcaattga tctcttgtta
     2461 tttaaaccaa cggtttcagg tcaatctttg gagtatttgt agcttctaat ttttgaaatg
     2521 actgaattaa gaatttggat gcttgctctt ttggttggtt tgcctaaaat ccagcccaca
     2581 atccagtcgt ctcttgggag agggaggtgc cttgcaaact ttcatataac gaatgtgcct
     2641 gaggctgctt aactctggac tagtctcaga tctcaaacct gcactacacg aggaggcata
     2701 cttttgcttc atctggacat ttagaatact gtaaccttgc tgccgttctg ttagattgct
     2761 aactacgtcc cccgtctcca atttggctct ccttaggcga taggatttgt tgtttttaag
     2821 gcaataaact tgacaacacc agaatccaag ttttacttga aaagctcggc agaatacaca
     2881 gtggtgtgaa aaaaacaaca gaaagggttt ttttgtgcaa tgacaaacgg taaaaatgct
     2941 gtaaacgttg aagaataaat atttccaacg caagaacctc ctgcttgact gtgtattatg
     3001 aacccaggaa accttagaag tttcaaattt ggagtcaaga ccttcaagaa ggtgggctcc
     3061 ggggatggta ccattgctct ttcctagcta accctagata cggcagctct ttaatgtact
     3121 ttaaaaagca aatatatatt actaaggaaa gaagttattt ataattgcct tgtcataatc
     3181 gttaaggtgt tctagaacca tttgcataca atttaatgta attccattcc attctgttgt
     3241 ttacacaatt actcaaagat gactgcaaag gtaaacggaa aaataaaagt gtattgcaca
     3301 gtg
//
