LOCUS       NM_021537               3254 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus serine/threonine kinase 25 (yeast) (Stk25), mRNA.
ACCESSION   NM_021537
VERSION     NM_021537.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Yang,X., Wu,S.T., Gao,R., Wang,R., Wang,Y., Dong,Z., Wang,L.,
            Qi,C., Wang,X., Schmitz,M.L., Liu,R., Han,Z., Wang,L. and Zheng,X.
  TITLE     Release of STK24/25 suppression on MEKK3 signaling in endothelial
            cells confers cerebral cavernous malformation
  JOURNAL   JCI Insight 8 (5), e160372 (2023)
   PUBMED   36692953
  REMARK    GeneRIF: Release of STK24/25 suppression on MEKK3 signaling in
            endothelial cells confers cerebral cavernous malformation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Zhang,X., Wang,B.Z., Kim,M., Nash,T.R., Liu,B., Rao,J., Lock,R.,
            Tamargo,M., Soni,R.K., Belov,J., Li,E., Vunjak-Novakovic,G. and
            Fine,B.
  TITLE     STK25 inhibits PKA signaling by phosphorylating PRKAR1A
  JOURNAL   Cell Rep 40 (7), 111203 (2022)
   PUBMED   35977512
  REMARK    GeneRIF: STK25 inhibits PKA signaling by phosphorylating PRKAR1A.
REFERENCE   3  (bases 1 to 3254)
  AUTHORS   Cansby,E., Kumari,S., Caputo,M., Xia,Y., Porosk,R., Robinson,J.,
            Wang,H., Olsson,B.M., Vallin,J., Grantham,J., Soomets,U.,
            Svensson,L.T., Sihlbom,C., Marschall,H.U., Edsfeldt,A.,
            Goncalves,I. and Mahlapuu,M.
  TITLE     Silencing of STE20-type kinase STK25 in human aortic endothelial
            and smooth muscle cells is atheroprotective
  JOURNAL   Commun Biol 5 (1), 379 (2022)
   PUBMED   35440683
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3254)
  AUTHORS   Sartages,M., Garcia-Colomer,M., Iglesias,C., Howell,B.W., Macia,M.,
            Pena,P., Pombo,C.M. and Zalvide,J.
  TITLE     GCKIII (Germinal Center Kinase III) Kinases STK24 and STK25
            (Serine/Threonine Kinase 24 and 25) Inhibit Cavernoma Development
  JOURNAL   Stroke 53 (3), 976-986 (2022)
   PUBMED   35130716
  REMARK    GeneRIF: GCKIII (Germinal Center Kinase III) Kinases STK24 and
            STK25 (Serine/Threonine Kinase 24 and 25) Inhibit Cavernoma
            Development.
REFERENCE   5  (bases 1 to 3254)
  AUTHORS   Kurhe,Y., Caputo,M., Cansby,E., Xia,Y., Kumari,S., Anand,S.K.,
            Howell,B.W., Marschall,H.U. and Mahlapuu,M.
  TITLE     Antagonizing STK25 Signaling Suppresses the Development of
            Hepatocellular Carcinoma Through Targeting Metabolic, Inflammatory,
            and Pro-Oncogenic Pathways
  JOURNAL   Cell Mol Gastroenterol Hepatol 13 (2), 405-423 (2022)
   PUBMED   34624527
REFERENCE   6  (bases 1 to 3254)
  AUTHORS   de Ledesma,A.M., Desai,A.N., Bolivar,V.J., Symula,D.J. and
            Flaherty,L.
  TITLE     Two new behavioral QTLs, Emo4 and Reb1, map to mouse Chromosome 1:
            Congenic strains and candidate gene identification studies
  JOURNAL   Mamm Genome 17 (2), 111-118 (2006)
   PUBMED   16465591
REFERENCE   7  (bases 1 to 3254)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   8  (bases 1 to 3254)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 3254)
  AUTHORS   Davids,M.S., Crawford,E., Weremowicz,S., Morton,C.C.,
            Copeland,N.G., Gilbert,D.J., Jenkins,N.A., Phelan,M.C., Comb,M.J.
            and Melnick,M.B.
  TITLE     STK25 is a candidate gene for pseudopseudohypoparathyroidism
  JOURNAL   Genomics 77 (1-2), 2-4 (2001)
   PUBMED   11543625
REFERENCE   10 (bases 1 to 3254)
  AUTHORS   Osada,S., Izawa,M., Saito,R., Mizuno,K., Suzuki,A., Hirai,S. and
            Ohno,S.
  TITLE     YSK1, a novel mammalian protein kinase structurally related to
            Ste20 and SPS1, but is not involved in the known MAPK pathways
  JOURNAL   Oncogene 14 (17), 2047-2057 (1997)
   PUBMED   9160885
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CF914439.1, AK148041.1, AK152917.1 and AK165787.1.
            
            On Mar 10, 2006 this sequence version replaced NM_021537.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK148041.1, BC071218.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                CF914439.1         1-27
            28-2003             AK148041.1         2-1977
            2004-2097           AK152917.1         1947-2040
            2098-2760           AK148041.1         2070-2732
            2761-2952           AK165787.1         563-754
            2953-3254           AK148041.1         2925-3226
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 47.25 cM"
     gene            1..3254
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /note="serine/threonine kinase 25 (yeast)"
                     /db_xref="GeneID:59041"
                     /db_xref="MGI:MGI:1891699"
     exon            1..181
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /inference="alignment:Splign:2.1.0"
     exon            182..267
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /inference="alignment:Splign:2.1.0"
     CDS             238..1518
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /EC_number="2.7.11.1"
                     /note="Ste20-like kinase; SOK-1; ste20/oxidant stress
                     response kinase 1; sterile 20/oxidant stress-response
                     kinase 1; Yeast Sps1/Ste20-related kinase 1;
                     serine/threonine kinase 25 (Ste20, yeast homolog)"
                     /codon_start=1
                     /product="serine/threonine-protein kinase 25"
                     /protein_id="NP_067512.3"
                     /db_xref="CCDS:CCDS15192.1"
                     /db_xref="GeneID:59041"
                     /db_xref="MGI:MGI:1891699"
                     /translation="MAHLRGFAHQHSRVDPEELFTKLDRIGKGSFGEVYKGIDNHTKE
                     VVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPYITRYFGSYLKSTKLWIIMEYLGGGS
                     ALDLLKPGPLEETYIATILREILKGLDYLHSERKIHRDIKAANVLLSEQGDVKLADFG
                     VAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDFKADIWSLGITAIELAKGEPPNSDL
                     HPMRVLFLIPKNNPPTLEGHHSKPFKEFVEACLNKDPRFRPTAKELLKHKFITRYTKK
                     TSFLTELIDRYKRWKSEGHGEESSSEDSDIDGEAEDGEQGPIWTFPPTIRPSPHSKLH
                     KGTALHSSQKPAEPIKRQPRSQCLSTLVRPVFGELKEKHKQSGGSVGALEELENAFSL
                     AEESCPGISDKLMVHLVERVQRFSHSRNHLTSTR"
     exon            268..498
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /inference="alignment:Splign:2.1.0"
     exon            499..555
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /inference="alignment:Splign:2.1.0"
     exon            556..664
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /inference="alignment:Splign:2.1.0"
     exon            665..822
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /inference="alignment:Splign:2.1.0"
     exon            823..1008
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1154
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1269
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1341
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1478
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1479..3254
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2080..2085
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /note="hexamer: AATAAA"
     polyA_site      2100
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /note="major polyA site"
     regulatory      2842..2847
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
                     /note="hexamer: AATACA"
     polyA_site      2876
                     /gene="Stk25"
                     /gene_synonym="1500019J11Rik; Ste20-like; Ysk1"
ORIGIN      
        1 ggggcgggct gcgggactcc tagtcccaca gagcttcgcg ctcgccacga cgccctgctc
       61 agagccgctg cctccacctc acgggctggg aggcctgggc gttccgggag accgaattgc
      121 ggtgtgagcc gccgtgggga ctgcgaagcg ggcccggagt ccgagatgga gccgcccgga
      181 ggtgcttgag gcgagcgggc ggagcggctg cgacggactg gtcacgtcag cggggacatg
      241 gctcacctcc ggggcttcgc gcaccagcat tctcgtgtgg acccagagga actcttcacc
      301 aagcttgacc gcattggcaa aggctcattt ggggaggtgt acaaggggat cgacaaccac
      361 accaaggaag tggtggccat caagatcatt gacttggaag aggccgagga tgaaattgag
      421 gacatccaac aggagatcac tgtgctcagc caatgtgaca gtccttatat cacccgctac
      481 ttcggctcct acctgaagag caccaagctg tggattatca tggagtacct gggtggtggc
      541 tctgcactgg acttgctgaa acctggtccc ctggaagaga cctatattgc caccatcttg
      601 cgggagatcc tgaaaggcct ggattatctg cactcagagc gcaagatcca ccgagatatc
      661 aaagctgcca acgtgctgct ctcggagcag ggtgatgtga agctggcaga cttcggtgtg
      721 gctgggcagc tcacagacac acaaatcaag aggaacacat ttgtgggtac acccttctgg
      781 atggcacctg aggttatcaa gcagtcggcc tatgacttca aggctgacat ctggtccctg
      841 ggcatcacag ccattgagct ggccaagggg gagccgccaa actctgatct ccaccctatg
      901 cgagtcctgt tcctgattcc caagaacaac ccacccacat tggagggcca ccacagcaaa
      961 cccttcaagg agtttgtaga ggcctgcctc aataaggacc cacgattccg acccactgcc
     1021 aaggagctcc tgaagcacaa attcatcaca cgatacacca agaagacctc ctttctcact
     1081 gaacttattg accgatataa gcgctggaag tctgaggggc atggagagga gtccagttct
     1141 gaggattctg acattgatgg cgaggctgag gatggggagc agggtcccat ctggacgttt
     1201 cctccaacca tccggccaag tccacatagc aagctgcaca agggaactgc tctgcacagc
     1261 tcacagaagc ctgcagagcc tatcaagaga cagccaaggt cccagtgctt gtccacacta
     1321 gtccgtcctg tctttggaga gctcaaagag aagcacaagc agagcggtgg gagcgtgggt
     1381 gccctggagg agctagagaa tgccttcagc ctggctgagg aatcctgtcc tggcatctca
     1441 gacaagctga tggtgcacct ggtggagcgt gtgcagaggt tctcacacag caggaaccac
     1501 ctgacatcta cccgctgaac aaccaactga atgcactact cttcacagag agggagaaga
     1561 atgctgtttg ttttgagctg taaaaggact gacttggaag ccctcttgtg ttgtgttaca
     1621 actgtaaaga ctgaccagcc tgtaggcttc acaagccagg ttaccttctt tctgccctac
     1681 acagcagccc cttgacacct gtgttcactc cttggttgtc tgaagctatg ctaggcacct
     1741 gggagccctt gcagaactgg gcagcaactt gggaggctgg tatgaccttc tatatagact
     1801 cctcagtccc tgcagcaggt agaccaagga gcatagtaac tattgggcag ctttggctct
     1861 tcgtctacac cagggacatt gtcttgaggg ctcatagggg cccaagatgg tcatcatttg
     1921 ccttgtggtg tcagttcagg tactgtgtgt tttcatgagt actcgaatcg tccagaatat
     1981 tttgggtttt tttttttttt tttttaggaa aattgaactt cctgagtatt ctgacattaa
     2041 gtgttagttt taatagagca ttgaaaaccc taccatttca ataaagacct ggattggaaa
     2101 tgttctgatg tacagagcct ggccagacag gatttctata gcttctgagc tattgtcata
     2161 taacctaaca ctttctggtg gccttctgaa gaacaaagta gggcaaaggg gatgctggtg
     2221 ggtgggggag atgttctttt attttaagtc acatgggcat gtgcgatggg aaccagagca
     2281 gcttttgctg atctggagac taacccctag agtaatatga gctcatcaca taagcctgag
     2341 tatatagtgc tagcctgagg ctacaggtag aagaagaagc aatccaacct gtatttaggg
     2401 ctagtcctca gcagggctat gttcatgaaa ctaaggacct tcacttcctt ttcacggatc
     2461 tcagacatgt gccccagcca tcatgagtct tcattgtttc tggaggttgg gggcagcaga
     2521 acccctccta gagtttttat gaggcccatt ccagggtcag tgccacctat ctggtaggag
     2581 gatgccagtt attaagtgcc tgggatgatg cttacagtcc ctgcaagcct ctgtcagagg
     2641 ttcttgtcca agcagggtga agggcatgcc tccttttctc tgatctctgc ctccagccct
     2701 ggagagtgat gtgagcagtc ctgagtgaaa cttgggggtc tccctggtga gctgctggcc
     2761 cttttgatga cgtccatcca cctgtgaaaa gggtcaaacc agtcggcata aattcacata
     2821 ccttatcaag agtgcctaaa aaatacagct agttcttgct gtgcaagcat gaggaactga
     2881 gtttcatcct tagcccctga gtacaaagct agccatgatg ttcactccta atccagcact
     2941 gaggaggtgg agctggagat ggatttgctg ctcagccagc cagcctaatc agcaagacac
     3001 ctgtctccta aaacaaggca gattgttcca gtgcaaggct tcagcagcta acacttgcca
     3061 tgcaagcctg gcaacctgaa ttcaatcccc agaacccaca taaaggtacg tggcaaaact
     3121 aaccccacat agtagtcctc tgacctgcac actcaagtgg cacacgtgtc cttcccacaa
     3181 cttaaaaaaa aaaggctcca gaagtccttt ggtcattgta tgtatgcaca catgtaaaca
     3241 tgaacatatc actg
//
