LOCUS       NM_009288               5082 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus serine/threonine kinase 10 (Stk10), transcript variant
            1, mRNA.
ACCESSION   NM_009288
VERSION     NM_009288.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Branco,M.R., King,M., Perez-Garcia,V., Bogutz,A.B., Caley,M.,
            Fineberg,E., Lefebvre,L., Cook,S.J., Dean,W., Hemberger,M. and
            Reik,W.
  TITLE     Maternal DNA Methylation Regulates Early Trophoblast Development
  JOURNAL   Dev Cell 36 (2), 152-163 (2016)
   PUBMED   26812015
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Edgar,A.J., Trost,M., Watts,C. and Zaru,R.
  TITLE     A combination of SILAC and nucleotide acyl phosphate labelling
            reveals unexpected targets of the Rsk inhibitor BI-D1870
  JOURNAL   Biosci Rep 34 (1), e00091 (2014)
   PUBMED   27919044
  REMARK    GeneRIF: kinases, including Slk, Lok and Mst1, are inhibited by
            BI-D1870 but to a much lesser extent by BIX 02565 and that
            phosphorylation of some of their substrates is blocked by BI-D1870
            in living cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5082)
  AUTHORS   Belkina,N.V., Liu,Y., Hao,J.J., Karasuyama,H. and Shaw,S.
  TITLE     LOK is a major ERM kinase in resting lymphocytes and regulates
            cytoskeletal rearrangement through ERM phosphorylation
  JOURNAL   Proc Natl Acad Sci U S A 106 (12), 4707-4712 (2009)
   PUBMED   19255442
  REMARK    GeneRIF: These studies identify a new ERM kinase of importance in
            lymphocytes and confirm the role of ezrin-radixin-moesin
            phosphorylation in regulating cell shape and motility.
REFERENCE   4  (bases 1 to 5082)
  AUTHORS   Pike,A.C., Rellos,P., Niesen,F.H., Turnbull,A., Oliver,A.W.,
            Parker,S.A., Turk,B.E., Pearl,L.H. and Knapp,S.
  TITLE     Activation segment dimerization: a mechanism for kinase
            autophosphorylation of non-consensus sites
  JOURNAL   EMBO J 27 (4), 704-714 (2008)
   PUBMED   18239682
REFERENCE   5  (bases 1 to 5082)
  AUTHORS   Walter,S.A., Cutler,R.E. Jr., Martinez,R., Gishizky,M. and
            Hill,R.J.
  TITLE     Stk10, a new member of the polo-like kinase kinase family highly
            expressed in hematopoietic tissue
  JOURNAL   J Biol Chem 278 (20), 18221-18228 (2003)
   PUBMED   12639966
  REMARK    GeneRIF: Stk10 is a novel polo-like kinase kinase that cooperates
            with hSlk to regulate Plk1 function in human cells
REFERENCE   6  (bases 1 to 5082)
  AUTHORS   Endo,J., Toyama-Sorimachi,N., Taya,C., Kuramochi-Miyagawa,S.,
            Nagata,K., Kuida,K., Takashi,T., Yonekawa,H., Yoshizawa,Y.,
            Miyasaka,N. and Karasuyama,H.
  TITLE     Deficiency of a STE20/PAK family kinase LOK leads to the
            acceleration of LFA-1 clustering and cell adhesion of activated
            lymphocytes
  JOURNAL   FEBS Lett 468 (2-3), 234-238 (2000)
   PUBMED   10692593
REFERENCE   7  (bases 1 to 5082)
  AUTHORS   Kuramochi,S., Matsuda,Y., Okamoto,M., Kitamura,F., Yonekawa,H. and
            Karasuyama,H.
  TITLE     Molecular cloning of the human gene STK10 encoding
            lymphocyte-oriented kinase, and comparative chromosomal mapping of
            the human, mouse, and rat homologues
  JOURNAL   Immunogenetics 49 (5), 369-375 (1999)
   PUBMED   10199912
REFERENCE   8  (bases 1 to 5082)
  AUTHORS   Kuramochi,S., Moriguchi,T., Kuida,K., Endo,J., Semba,K., Nishida,E.
            and Karasuyama,H.
  TITLE     LOK is a novel mouse STE20-like protein kinase that is expressed
            predominantly in lymphocytes
  JOURNAL   J Biol Chem 272 (36), 22679-22684 (1997)
   PUBMED   9278426
REFERENCE   9  (bases 1 to 5082)
  AUTHORS   Yanagisawa,M., Mukouyama,Y., Watanabe,T., Obinata,M. and Matsui,Y.
  TITLE     A novel serine/threonine kinase gene, Gek1, is expressed in meiotic
            testicular germ cells and primordial germ cells
  JOURNAL   Mol Reprod Dev 45 (4), 411-420 (1996)
   PUBMED   8956278
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY207613.1, BC128363.1,
            BC096624.1, AL669844.20 and AA182088.1.
            
            On Feb 28, 2007 this sequence version replaced NM_009288.1.
            
            Summary: This gene encodes a member of the Ste20 family of
            serine/threonine protein kinases, and is similar to several known
            polo-like kinase kinases. Mice deficient for this gene product are
            viable, but exhibit altered integrin-mediated lymphocyte adhesion
            characteristics. The orthologous gene product in humans can
            associate with and phosphorylate polo-like kinase 1, and
            overexpression of a kinase-dead version of the protein interferes
            with normal cell cycle progression. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: D89728.1, AK148118.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-114               BY207613.1         2-115
            115-1261            BC128363.1         1-1147
            1262-1602           BC096624.1         1347-1687
            1603-2291           BC128363.1         1489-2177
            2292-2831           BC096624.1         2377-2916
            2832-3241           BC128363.1         2718-3127
            3242-4788           AL669844.20        162997-164543
            4789-5082           AA182088.1         169-462
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 18.97 cM"
     gene            1..5082
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /note="serine/threonine kinase 10"
                     /db_xref="GeneID:20868"
                     /db_xref="MGI:MGI:1099439"
     exon            1..294
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..3039
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     lymphocyte-oriented kinase; serine/threonine-protein
                     kinase 10; germ cell kinase 1"
                     /codon_start=1
                     /product="serine/threonine-protein kinase 10 isoform 1"
                     /protein_id="NP_033314.2"
                     /db_xref="CCDS:CCDS24529.1"
                     /db_xref="GeneID:20868"
                     /db_xref="MGI:MGI:1099439"
                     /translation="MAFANFRRILRLSTFEKRKSREYEHVRRDLDPNDVWEIVGELGD
                     GAFGKVYKAKNKETGALAAAKVIETKSEEELEDYIVEIEILATCDHPYIVKLLGAYYY
                     DGKLWIMIEFCPGGAVDAIMLELDRGLTEPQIQVVCRQMLEALNFLHGKRIIHRDLKA
                     GNVLMTLEGDIRLADFGVSAKNLKTLQKRDSFIGTPYWMAPEVVLCETMKDAPYDYKA
                     DIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDPPTLLTPSKWSVEFRDFLKIALD
                     KNPETRPSAAQLLQHPFVSRVTSNKALRELVAEAKAEVMEEIEDGREDGEEEDAVDAV
                     PPLVNHTQDSANVTQPSLDSNKLLQDSSTPLPPSQPQEPVSGSCSQPSGDGPLQTTSP
                     ADGLSKNDNDLKVPVPLRKSRPLSMDARIQMDEEKQIPDQDENPSPAASKSQKANQSR
                     PNSSALETLGGEALTNGGLELPSSVTPSHSKRASDCSNLSTSESMDYGTSLSADLSLN
                     KETGSLSLKGSKLHNKTLKRTRRFVVDGVEVSITTSKIISEDEKKDEEMRFLRRQELR
                     ELRLLQKEEHRNQTQLSSKHELQLEQMHKRFEQEINAKKKFYDVELENLERQQKQQVE
                     KMEQDHSVRRKEEAKRIRLEQDRDYAKFQEQLKQMKKEVKSEVEKLPRQQRKESMKQK
                     MEEHSQKKQRLDRDFVAKQKEDLELAMRKLTTENRREICDKERDCLSKKQELLRDREA
                     ALWEMEEHQLQERHQLVKQQLKDQYFLQRHDLLRKHEKEREQMQRYNQRMMEQLKVRQ
                     QQEKARLPKIQRSDGKTRMAMYKKSLHINGAGSASEQREKIKQFSQQEEKRQKAERLQ
                     QQQKHENQMRDMVAQCESNMSELQQLQNEKCHLLVEHETQKLKALDESHNQSLKEWRD
                     KLRPRKKALEEDLNQKKREQEMFFKLSEEAEPRPTTPSKASNFFPYSSGDAS"
     exon            295..459
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            460..508
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            509..658
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            659..731
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            732..926
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1008
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1143
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1689
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..1820
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..1944
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..2124
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            2125..2217
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            2218..2347
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            2348..2472
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            2473..2661
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            2662..2787
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            2788..2901
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     exon            2902..5073
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3353
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /note="major polyA site"
     regulatory      5049..5054
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /note="hexamer: AATAAA"
     regulatory      5057..5062
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /note="hexamer: AATAAG"
     polyA_site      5070
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
     polyA_site      5073
                     /gene="Stk10"
                     /gene_synonym="Gek1; Lok; mKIAA4026"
                     /note="major polyA site"
ORIGIN      
        1 taggaggggg cggggcgggg cgggcgggcc gcggttcctg cagctccctg tggccgctgg
       61 cgcactcggc gggtgctgcg ctgcgaggag ggcggctctc cgaagccgcg ggtcctgtcg
      121 tcgcgctcct catccagcat ggctttcgcc aatttccgcc gcatcttgcg gttatccacc
      181 ttcgagaaga gaaagtcccg tgaatatgag cacgtccgcc gggacctgga ccccaacgat
      241 gtatgggaga tcgtgggcga gctgggagac ggcgccttcg gcaaagtcta caaggccaaa
      301 aacaaggaga ccggtgccct ggcagcagcc aaagtcatag agaccaagag cgaggaggag
      361 ctggaggact acatcgtgga gatagagatc ctggccacct gtgaccaccc gtatatcgtg
      421 aagctcctgg gggcctatta ctatgatggg aagctctgga tcatgattga gttctgccct
      481 gggggagctg tggatgccat catgctggag ctggatcgag gcctcactga gccccagatt
      541 caggtcgtat gccgccagat gttggaggct ctcaacttcc tgcacggcaa gaggatcatc
      601 caccgagacc tgaaagctgg caacgtgctt atgacccttg agggggacat caggctggct
      661 gattttggtg tgtctgccaa gaatctgaaa actctgcaaa agcgagactc cttcataggg
      721 acaccttact ggatggcccc tgaggtggtg ctgtgtgaaa ccatgaagga cgccccctat
      781 gactacaagg ctgacatctg gtccttgggc atcacgctga tcgagatggc gcagatcgag
      841 cccccacacc acgagctcaa ccccatgcgg gtcctgctca agattgccaa gtcggaccct
      901 cctaccctgc ttacaccctc caagtggtct gtggagttcc gagactttct gaagatagcc
      961 ttggataaga acccagaaac ccggcccagt gctgcacagc tgctgcagca tcccttcgtc
     1021 agcagagtca ccagcaataa ggctcttcgg gagctggtgg ccgaggcaaa ggcggaggta
     1081 atggaggaga ttgaggatgg cagggaggac ggagaagagg aggatgctgt ggatgctgtt
     1141 ccgcccctgg tcaaccacac tcaggactct gccaatgtaa ctcagccaag cctcgactcc
     1201 aacaagcttc tccaggattc ttccaccccc ctgcctccca gccagcctca ggagcctgtg
     1261 agcgggtcct gtagccaacc ctctggggat ggacccctcc aaaccaccag ccctgcagat
     1321 gggctctcca agaatgacaa tgacttaaag gtgcctgttc ccctccggaa gtcccggcca
     1381 ctgtccatgg atgccagaat tcagatggat gaagagaaac aaatccctga ccaggatgag
     1441 aacccgagtc ctgcagccag caagtcccaa aaggccaacc agagccgacc taacagcagt
     1501 gccctggaga ccttgggtgg cgaggctctg accaacggtg gcctggaact ccccagctct
     1561 gtcactccaa gccattctaa gagggcctcg gactgtagca acctgtctac ctcagagagc
     1621 atggactatg gcacctccct gtctgctgac ctgtcactga acaaagaaac gggctcattg
     1681 tctctcaagg gctcaaaact gcacaataag accctgaaac ggacccgccg gtttgtggtg
     1741 gatggtgtgg aggtgagcat caccacctcc aagatcatca gcgaggatga gaagaaagac
     1801 gaggagatga gatttctcag gcgccaggag ctccgagagc ttcggcttct gcagaaagaa
     1861 gaacatcgga accagacgca gctgagcagc aagcacgagc tgcagctgga gcagatgcac
     1921 aaacgttttg aacaagaaat caacgccaag aagaaattct atgacgtgga gctagaaaac
     1981 ctggagcggc agcagaagca gcaggtagag aagatggagc aggaccacag cgtgcgccgc
     2041 aaagaggagg ccaagcggat ccgcctggag caggatcgag actacgccaa gttccaagag
     2101 caactcaagc aaatgaagaa ggaggtgaag agtgaggttg agaaactgcc ccggcagcag
     2161 cggaaggaga gcatgaagca gaagatggag gaacactctc agaagaaaca gcggctggac
     2221 cgggactttg tagccaagca gaaggaggac ctggagctgg ccatgaggaa gctcaccacg
     2281 gaaaacaggc gtgagatctg cgacaaggaa cgagattgcc ttagcaagaa gcaggagctc
     2341 ctccgagacc gtgaggcggc cctgtgggag atggaggagc accagttgca ggagcgacat
     2401 cagctggtga agcagcaact gaaggaccag tacttcctgc agcggcatga cctgctgcgt
     2461 aagcatgaga aggagcggga acagatgcag cgctacaacc agcgcatgat ggagcagctg
     2521 aaggtcagac agcaacagga gaaggcgcgg ctacccaaga tccagaggag cgacggcaag
     2581 acgcgcatgg ccatgtacaa gaagagcctg cacatcaacg gtgcgggcag tgcctctgag
     2641 cagcgcgaga agatcaagca gttctcccag caggaggaga agaggcagaa ggcggagagg
     2701 ctgcagcagc agcagaaaca cgagaaccag atgcgagaca tggtggcgca gtgtgaaagc
     2761 aacatgagcg agctacagca gctgcagaat gaaaagtgtc atctgttggt agagcatgaa
     2821 acccagaagc tgaaggccct ggatgagagc cacaaccaga gcctgaagga atggcgagac
     2881 aagcttcggc cacgcaaaaa ggccctggaa gaggatttga accagaagaa gcgggaacag
     2941 gaaatgttct tcaaattaag cgaggaggca gagcccagac ccaccacacc cagcaaagcc
     3001 agcaacttct tcccctacag ctctggggat gcttcctaac acccacaagc ctgggctgcg
     3061 gtgggcagcg tggctactgg ggcccccaag cctctatgaa caagtgactc aggacctctt
     3121 cctcttgctt ctgtgccagc tcaattaccc atccccctgc agcgccccag ttgtccacag
     3181 tgccaccttg gtgcacggtg acctcctcaa gactcagatt cccatcacct aggagtgatt
     3241 tagactgttg aggtcagggg ccaggcagga tgggtgaacc cctcctgttt gcccaaacac
     3301 cagctctact gtctgtgggc acaagcgcta ctaatgacat caccgcgaac gcatccttat
     3361 tgtgatcctt gtgggttttt tgtttgtttg tctgtttgtt tgtttgcctt cagtaattcc
     3421 tcacagtgtc gggaaacatc cctaagagcc acttgggttc tcagcagcca gctcccgggt
     3481 gtccccattc ccttgtttca catagctgac tttgctttgt gtatgtcggg gtagggagcg
     3541 tggagaagga tgccagagat gcctgttctg ctgattctga cattgtatcc catcttattt
     3601 tcctctccct gtgagacatg caatacacac acacacacac acacacacac acacacacac
     3661 acacacacac acacacactg caaatacata acttggcccc cctgacacca cccaggctgc
     3721 ctctgctgtg acctcctagg accaatgcat tggaacctgc ttgcttgcct cctgagcctg
     3781 atccagggcc actgagcatg tcaggtggag cagctggtgg ggccatgctg agtgctggcg
     3841 ccagaaccca gagaaggcac aggctgtact gccagcctgc aactcatttg gctgcacaca
     3901 ggatcccgtg ttcaggggta cacccccctc cacacttgac ttctgctgcc tagcacgtgc
     3961 caatctcacc cttgcccgct tgtctgcagg tacccaggaa ggcttttgac ttctgcccta
     4021 gtcctgtact ggagtcccac tgtacatttc cactaagctt tggacttctc tgttcaggaa
     4081 gaactgttcc ccaccctcag gaacatgctg cgctcaggga aatgtggagc ctccatgact
     4141 gggccttcgg gctgctcagg tagcctccca agaggctgag ccccatagaa gaaaagcagc
     4201 ctaagtgctc agttacagta caggcaaatg gctggctgcc tcagacaccc tctttttaaa
     4261 aagccccttc ccagggacaa tggggtgggg tgggggaggg gggacccagg cggttgagtc
     4321 tggcgagtgg ctgagacctt agcagtattt ctctgtcact gcccgatgct gaagcaactt
     4381 tctaaaaagt ttcctcaaat gaaaactggg tgcttgggag ggagtgggct agaaaaaaaa
     4441 taggaaaaca ggtcttggga ctcccagatc tagtgtgcag taaagaagct cacagaaccc
     4501 caggaaggcc atagttctca ggggagcgag tgagccttcg cttgctttca ggcctcacat
     4561 caaggagttg actcagaggg cagatgggcc aggccacgcc acaggaggcc gggcagggat
     4621 acttgctttc aggggacttg acagtggcta ttaggcaaaa tgctagtttt gtggttcata
     4681 taacagtctt tgaccgctcc ctgacatcat tcgtaatgtt ccagtccttt gttgaatctg
     4741 cacttagtac cacctgagcc ctggtagctc tgctgtgcct tttggagtgg ggtctagcct
     4801 agtcttccag cctcataatt atagcgtaag tgcaatgcct gccaccacca agggcccacg
     4861 cagtattcct catgccctgt gctaatcttt tctgtatagg aacaggcaga agtgttttta
     4921 gcgcagtaaa tataactaac ttatatttcc cttcatgaag gatggaaata acgtgtatgc
     4981 atttccaagc accgtggata tttgtaaact gttctctgca aacaaaaaaa gaatgtaaat
     5041 atgcttctaa taaagtaata aggtgatttg caaaaaaaaa aa
//
