LOCUS       NM_145934               2352 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus signal transducing adaptor family member 2 (Stap2),
            transcript variant 1, mRNA.
ACCESSION   NM_145934 XM_017317201
VERSION     NM_145934.3
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Sekine,Y., Kikkawa,K., Honda,S., Sasaki,Y., Kawahara,S.,
            Mizushima,A., Togi,S., Fujimuro,M., Oritani,K. and Matsuda,T.
  TITLE     STAP-2 facilitates insulin signaling through binding to CAP/c-Cbl
            and regulates adipocyte differentiation
  JOURNAL   Sci Rep 14 (1), 5799 (2024)
   PUBMED   38461189
  REMARK    GeneRIF: STAP-2 facilitates insulin signaling through binding to
            CAP/c-Cbl and regulates adipocyte differentiation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Kashiwakura,J.I., Kawahara,S., Inagaki,I., Inui,K., Saitoh,K.,
            Kagohashi,K., Sasaki,Y., Kobayashi,F., Kitai,Y., Muromoto,R.,
            Oritani,K. and Matsuda,T.
  TITLE     STAP-2 negatively regulates BCR-mediated B cell activation by
            recruiting tyrosine-protein kinase CSK to LYN
  JOURNAL   FEBS Lett 597 (19), 2433-2445 (2023)
   PUBMED   37669828
  REMARK    GeneRIF: STAP-2 negatively regulates BCR-mediated B cell activation
            by recruiting tyrosine-protein kinase CSK to LYN.
REFERENCE   3  (bases 1 to 2352)
  AUTHORS   Saitoh,K., Kashiwakura,J.I., Kagohashi,K., Sasaki,Y., Kawahara,S.,
            Sekine,Y., Kitai,Y., Muromoto,R., Ichii,M., Nakatsukasa,H.,
            Yoshimura,A., Oritani,K. and Matsuda,T.
  TITLE     STAP-2 Is a Novel Positive Regulator of TCR-Proximal Signals
  JOURNAL   J Immunol 209 (1), 57-68 (2022)
   PUBMED   35725273
  REMARK    GeneRIF: STAP-2 Is a Novel Positive Regulator of TCR-Proximal
            Signals.
REFERENCE   4  (bases 1 to 2352)
  AUTHORS   Kashiwakura,J.I., Koizumi,N., Saitoh,K., Kagohashi,K., Sasaki,Y.,
            Kobayashi,F., Kawahara,S., Yamauchi,Y., Kitai,Y., Muromoto,R.,
            Oritani,K. and Matsuda,T.
  TITLE     Signal-transducing adaptor protein-2 has a nonredundant role for
            IL-33-triggered mast cell activation
  JOURNAL   Biochem Biophys Res Commun 572, 80-85 (2021)
   PUBMED   34358967
  REMARK    GeneRIF: Signal-transducing adaptor protein-2 has a nonredundant
            role for IL-33-triggered mast cell activation.
REFERENCE   5  (bases 1 to 2352)
  AUTHORS   Saito,H., Ichii,M., Toda,J., Kitai,Y., Muromoto,R.,
            Kashiwakura,J.I., Saitoh,K., Tanimura,A., Yokota,T., Shibayama,H.,
            Matsuda,T., Oritani,K., Kanakura,Y. and Hosen,N.
  TITLE     Graft-versus-host disease develops in mice transplanted with
            lymphocyte-depleted bone marrow cells from signal-transducing
            adaptor protein-2 transgenic mice
  JOURNAL   Biochem Biophys Res Commun 537, 118-124 (2021)
   PUBMED   33388414
  REMARK    GeneRIF: Graft-versus-host disease develops in mice transplanted
            with lymphocyte-depleted bone marrow cells from signal-transducing
            adaptor protein-2 transgenic mice.
REFERENCE   6  (bases 1 to 2352)
  AUTHORS   Sekine,Y., Tsuji,S., Ikeda,O., Sugiyma,K., Oritani,K., Shimoda,K.,
            Muromoto,R., Ohbayashi,N., Yoshimura,A. and Matsuda,T.
  TITLE     Signal-transducing adaptor protein-2 regulates integrin-mediated T
            cell adhesion through protein degradation of focal adhesion kinase
  JOURNAL   J Immunol 179 (4), 2397-2407 (2007)
   PUBMED   17675501
  REMARK    GeneRIF: STAP-2 associates with FAK and enhances its degradation,
            proteasome inhibitors block FAK degradation, and STAP-2 recruits an
            endogenous E3 ubiquitin ligase, Cbl, to FAK.
REFERENCE   7  (bases 1 to 2352)
  AUTHORS   Ikeda,O., Sekine,Y., Kakisaka,M., Tsuji,S., Muromoto,R.,
            Ohbayashi,N., Oritani,K., Yoshimura,A. and Matsuda,T.
  TITLE     STAP-2 regulates c-Fms/M-CSF receptor signaling in murine
            macrophage Raw 264.7 cells
  JOURNAL   Biochem Biophys Res Commun 358 (3), 931-937 (2007)
   PUBMED   17512498
  REMARK    GeneRIF: STAP-2 directly binds to c-Fms and interferes with the
            PI3K signaling, which leads to macrophage motility, in Raw 264.7
            cells
REFERENCE   8  (bases 1 to 2352)
  AUTHORS   Sekine,Y., Yumioka,T., Yamamoto,T., Muromoto,R., Imoto,S.,
            Sugiyma,K., Oritani,K., Shimoda,K., Minoguchi,M., Akira,S.,
            Yoshimura,A. and Matsuda,T.
  TITLE     Modulation of TLR4 signaling by a novel adaptor protein
            signal-transducing adaptor protein-2 in macrophages
  JOURNAL   J Immunol 176 (1), 380-389 (2006)
   PUBMED   16365431
REFERENCE   9  (bases 1 to 2352)
  AUTHORS   Sekine,Y., Yamamoto,T., Yumioka,T., Sugiyama,K., Tsuji,S.,
            Oritani,K., Shimoda,K., Minoguchi,M., Yoshimura,A. and Matsuda,T.
  TITLE     Physical and functional interactions between STAP-2/BKS and STAT5
  JOURNAL   J Biol Chem 280 (9), 8188-8196 (2005)
   PUBMED   15611091
  REMARK    GeneRIF: STAP-2/BKS is a modulator of STAT5-mediated signaling
REFERENCE   10 (bases 1 to 2352)
  AUTHORS   Minoguchi,M., Minoguchi,S., Aki,D., Joo,A., Yamamoto,T.,
            Yumioka,T., Matsuda,T. and Yoshimura,A.
  TITLE     STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation
            in acute-phase response through its YXXQ motif
  JOURNAL   J Biol Chem 278 (13), 11182-11189 (2003)
   PUBMED   12540842
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT009719.10, AK050131.1 and AW049765.1.
            
            [WARNING] On Aug 5, 2024 this sequence was replaced by NM_145934.4.
            
            On or before Nov 10, 2017 this sequence version replaced
            XM_017317201.1, NM_145934.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660825.105133.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-720               CT009719.10        51605-52324         c
            721-829             CT009719.10        49774-49882         c
            830-2334            AK050131.1         2-1506
            2335-2352           AW049765.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 29.1 cM"
     gene            1..2352
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /note="signal transducing adaptor family member 2"
                     /db_xref="GeneID:106766"
                     /db_xref="MGI:MGI:2147039"
     exon            1..720
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            721..985
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             884..2119
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     signal-transducing adaptor protein 2"
                     /codon_start=1
                     /product="signal-transducing adaptor protein 2 isoform 1"
                     /protein_id="NP_666046.1"
                     /db_xref="CCDS:CCDS28889.1"
                     /db_xref="GeneID:106766"
                     /db_xref="MGI:MGI:2147039"
                     /translation="MATALSPPRGPKLKGAPPSHYYESFLEKKGPCDQDYRKFWAGLQ
                     GLAICFYNSNRDLQPLEKLDLRLFSKLRDEALLGSSRDTAYHFSLVLRDQEVKFKVES
                     LESCEMWKGFILTVVELRVPSNLTLLPGHLYMMAEVLTKEEVRRAAEVPWCFLQVSRL
                     EAQLLLERYPECGNLLLRPGGDGKDSVSVTTRQILNGSPVVKHYKVKRAGSKYVIDVE
                     DPFSCPSLEAVVNYFVTHTKRALVPFLLDEDYEKVLGFVDSDRENGESAWAVPSFRAS
                     GPALPANVLKPLPPVPVSVSSQEDKLPQLPPLPQLPDTDENYVTPIEDSPAAEYMNQD
                     VSLSSQAVPLKPKKPARLPAKPPKPSVVPKPDLKAITSVWTRKLGGSSSQASSLVTRL
                     GDITAELEEKLQKRRALEH"
     exon            986..1057
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1058..1180
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1237
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1338
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1339..1473
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1543
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1646
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1712
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1713..1886
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1887..1979
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1980..2054
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2055..2339
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2315..2320
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /note="hexamer: AATAAA"
     polyA_site      2339
                     /gene="Stap2"
                     /gene_synonym="STAP-2"
                     /note="major polyA site"
ORIGIN      
        1 gctgggattt gaactcctga ccttcggaag aacagtcggg tgctcttacc cactgagcca
       61 tctcaccagt ccgagaacca ctcttccctt agcccaggcc accagacttt gggctccata
      121 gtttaccgct tccaagtccc ggcacaatat gcaggtctaa ccatcttgta aacaggcaaa
      181 gccttgagag aggctccaag ttctgctcac tggacgtttt catcctgttt atcttccacc
      241 tcgatccaaa agggactttt ctatgatgta actactgcca catcctatcc ttgcgctaca
      301 acagtccttg gctcccggct aatttttctt ttccttcctt ccacttgcag tgatcctcct
      361 gctcagcccc ctcctgctta cgtcataggc ttgcatcccc acacctagct cccctgtggc
      421 ctttgacact ttgaagcaac tactgtggct tgacaatgta cagaaaccta aagataacat
      481 tggttgaaga taaaggaagg ttgtgccact cacagccact atctgggtcc ccatctccac
      541 ccttttacct gtgtaaagtc caggtgccct agctgggaac tgaagaaaat gagttcacaa
      601 tctgttctgc ctttgccttt tgaaaatagc atctcgcagg gcagtggtgg cctgtgatcc
      661 tagcacttgg gaggcagaag ccggtggatt tctaaattca acgccagcct ggtctacaga
      721 gagcccagaa ctaccgggat ttccgcagga tccagccccc gcccagaact gctagagagg
      781 gaggtgacag ctgactgagt tgaggaaact ccccaggaac ctgtttatcc acttcctggc
      841 cagactgagg ctcctactga aaggacaggg acccaggctg gccatggcca cagccctgag
      901 tccaccccgg ggtcccaagc tcaaaggtgc tccaccttca cactactacg agagcttcct
      961 agagaaaaag ggaccctgtg accaggatta caggaagttc tgggcaggcc tccagggttt
     1021 agccatctgt ttctacaata gcaatcggga cttgcagccc ctggaaaagc tggacctgag
     1081 gctgttttca aagctcagag atgaggctct gctgggaagc tcacgggaca ctgcctatca
     1141 cttcagcctg gttctccggg accaggaggt gaaattcaag gtggagagcc tggagtcttg
     1201 tgagatgtgg aaaggattta tcttgactgt ggtagagctc cgtgtcccat ctaacctgac
     1261 cctgctgccc ggacacctgt acatgatggc cgaggtcctg accaaagagg aggtacggag
     1321 ggcagccgag gtgccctggt gctttcttca ggtgagcagg ctcgaggcgc agctgctcct
     1381 ggagcgctac ccagagtgcg ggaacctgct tctgcggcct ggcggggatg gcaaggacag
     1441 tgtgtctgtc accacccggc agattctcaa tgggtctccc gtggtcaaac actacaaagt
     1501 gaagcgggca ggttctaagt atgtgatcga cgtggaagac ccgttttcct gcccctcgct
     1561 ggaagctgta gtcaactatt ttgtgacgca caccaagagg gcgctggtcc ccttcttgtt
     1621 ggatgaggac tatgagaagg ttctaggctt cgtggactcg gatcgggaga atggcgagag
     1681 tgcatgggct gtgccctcct tccgagcctc aggccctgcc ctccctgcta atgtcctgaa
     1741 gccactccca cctgtacctg tgtctgtgtc cagccaggaa gacaagttgc ctcaactacc
     1801 tcccctgcct cagttaccag acacggatga gaactacgtg actcccattg aagactcccc
     1861 agcagccgaa tacatgaatc aggatgtgtc tctctccagt caggcagtcc ctctgaagcc
     1921 caagaagccg gcaaggctcc cagcaaaacc cccaaagcca tcagttgtgc ctaagccaga
     1981 tctcaaagcc attaccagcg tttggaccag gaagctaggt ggaagctcgt cccaggcttc
     2041 ctcccttgtg acaagacttg gggatatcac cgctgagctg gaagagaaac tacagaagag
     2101 gagggcactg gaacactgat ccaggctccc agggatcagt gggctatctc tgggtacaat
     2161 agtcttcttc cagctctatc ctcactcagg ggataggaca cagccaaaat aacctccctc
     2221 aagtaaagca gggagagccc tgagaatttt ggtgctaccc tgagctgtat agtgagttcc
     2281 acggtagcct gtgctacaaa gtaagatctt gtttaataaa gctaaaacca aacctaaaaa
     2341 aaaaaaaaaa aa
//
