LOCUS       NM_019992               1225 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus signal transducing adaptor family member 1 (Stap1),
            transcript variant 1, mRNA.
ACCESSION   NM_019992 XM_917126 XM_924678 XM_924680 XM_924686
VERSION     NM_019992.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Kagohashi,K., Sasaki,Y., Ozawa,K., Tsuchiya,T., Kawahara,S.,
            Saitoh,K., Ichii,M., Toda,J., Harada,Y., Kubo,M., Kitai,Y.,
            Muromoto,R., Oritani,K., Kashiwakura,J.I. and Matsuda,T.
  TITLE     Role of Signal-Transducing Adaptor Protein-1 for T Cell Activation
            and Pathogenesis of Autoimmune Demyelination and Airway
            Inflammation
  JOURNAL   J Immunol 212 (6), 951-961 (2024)
   PUBMED   38315039
  REMARK    GeneRIF: Role of Signal-Transducing Adaptor Protein-1 for T Cell
            Activation and Pathogenesis of Autoimmune Demyelination and Airway
            Inflammation.
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1225)
  AUTHORS   Yang,X., Ji,C., Qi,Y., Huang,J., Hu,L., Zhou,Y., Zou,L., Xia,Y.,
            Tan,F., Yao,Y. and Chen,D.
  TITLE     Signal-transducing adaptor protein 1 (STAP1) in microglia promotes
            the malignant progression of glioma
  JOURNAL   J Neurooncol 164 (1), 127-139 (2023)
   PUBMED   37462801
  REMARK    GeneRIF: Signal-transducing adaptor protein 1 (STAP1) in microglia
            promotes the malignant progression of glioma.
REFERENCE   4  (bases 1 to 1225)
  AUTHORS   Kanuri,B., Fong,V., Haller,A., Hui,D.Y. and Patel,S.B.
  TITLE     Mice lacking global Stap1 expression do not manifest
            hypercholesterolemia
  JOURNAL   BMC Med Genet 21 (1), 234 (2020)
   PUBMED   33228548
  REMARK    GeneRIF: Mice lacking global Stap1 expression do not manifest
            hypercholesterolemia.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1225)
  AUTHORS   Kashiwakura,J.I., Saitoh,K., Ihara,T., Sasaki,Y., Kagohashi,K.,
            Enohara,S., Morioka,Y., Watarai,H., Muromoto,R., Kitai,Y.,
            Iwabuchi,K., Oritani,K. and Matsuda,T.
  TITLE     Expression of signal-transducing adaptor protein-1 attenuates
            experimental autoimmune hepatitis via down-regulating activation
            and homeostasis of invariant natural killer T cells
  JOURNAL   PLoS One 15 (11), e0241440 (2020)
   PUBMED   33175848
  REMARK    GeneRIF: Expression of signal-transducing adaptor protein-1
            attenuates experimental autoimmune hepatitis via down-regulating
            activation and homeostasis of invariant natural killer T cells.
            Erratum:[PLoS One. 2021 Apr 15;16(4):e0250536. doi:
            10.1371/journal.pone.0250536.. PMID: 33857256]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1225)
  AUTHORS   Lyssand,J.S., DeFino,M.C., Tang,X.B., Hertz,A.L., Feller,D.B.,
            Wacker,J.L., Adams,M.E. and Hague,C.
  TITLE     Blood pressure is regulated by an alpha1D-adrenergic
            receptor/dystrophin signalosome
  JOURNAL   J Biol Chem 283 (27), 18792-18800 (2008)
   PUBMED   18468998
REFERENCE   7  (bases 1 to 1225)
  AUTHORS   Ghazalpour,A., Doss,S., Zhang,B., Wang,S., Plaisier,C.,
            Castellanos,R., Brozell,A., Schadt,E.E., Drake,T.A., Lusis,A.J. and
            Horvath,S.
  TITLE     Integrating genetic and network analysis to characterize genes
            related to mouse weight
  JOURNAL   PLoS Genet 2 (8), e130 (2006)
   PUBMED   16934000
REFERENCE   8  (bases 1 to 1225)
  AUTHORS   Sollars,V.E., Pequignot,E., Rothstein,J.L. and Buchberg,A.M.
  TITLE     Analysis of expansion of myeloid progenitors in mice to identify
            leukemic susceptibility genes
  JOURNAL   Mamm Genome 17 (8), 808-821 (2006)
   PUBMED   16897342
REFERENCE   9  (bases 1 to 1225)
  AUTHORS   Yokohari,K., Yamashita,Y., Okada,S., Ohya,K., Oda,S., Hatano,M.,
            Mano,H., Hirasawa,H. and Tokuhisa,T.
  TITLE     Isoform-dependent interaction of BRDG1 with Tec kinase
  JOURNAL   Biochem Biophys Res Commun 289 (2), 414-420 (2001)
   PUBMED   11716489
REFERENCE   10 (bases 1 to 1225)
  AUTHORS   Masuhara,M., Nagao,K., Nishikawa,M., Sasaki,M., Yoshimura,A. and
            Osawa,M.
  TITLE     Molecular cloning of murine STAP-1, the stem-cell-specific adaptor
            protein containing PH and SH2 domains
  JOURNAL   Biochem Biophys Res Commun 268 (3), 697-703 (2000)
   PUBMED   10679268
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK162655.1, AK041474.1 and BF660908.1.
            
            On Jul 16, 2015 this sequence version replaced NM_019992.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK162655.1, AK041474.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-82                AK162655.1         1-82
            83-1218             AK041474.1         2-1137
            1219-1225           BF660908.1         1-7                 c
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 43.56 cM"
     gene            1..1225
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
                     /note="signal transducing adaptor family member 1"
                     /db_xref="GeneID:56792"
                     /db_xref="MGI:MGI:1926193"
     exon            1..212
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..986
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
                     /note="isoform 1 is encoded by transcript variant 1; stem
                     cell adaptor protein STAP-1; signal-transducing adaptor
                     protein 1; BCR downstream signaling 1"
                     /codon_start=1
                     /product="signal-transducing adaptor protein 1 isoform 1"
                     /protein_id="NP_064376.1"
                     /db_xref="CCDS:CCDS19377.1"
                     /db_xref="GeneID:56792"
                     /db_xref="MGI:MGI:1926193"
                     /translation="MMAKKPPKPAPRRIFQERLKITALPLYFEGFLLVKRSDHQEYKH
                     YWTELRGTTLFFYTDKKSTIYVGKLDIIDLVCLTGQHSTEKNCAKFTLVLPKEEVHVK
                     TENTESGEEWRGFILTVTELTVPQHVSLLPGQVIRLHEVLEREKKRRIETDQLPLMPP
                     EKEKEPVQDYADVLNPLPECFYAVSRKEATAMLEKNPSWGNMILRPGSDSKNYSITIR
                     QEIEMPRIKHFKVTRTGNNYTIELEKPVTLPNLFSVIDYFVKETRGNLRPFIHSADDN
                     FGQDPNIEDRSEKFKKNPHNA"
     exon            213..284
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            285..398
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            399..455
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            456..628
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            629..757
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            758..827
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            828..924
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1225
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1095..1100
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
                     /note="hexamer: AATAAA"
     polyA_site      1116
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
     regulatory      1195..1200
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
                     /note="hexamer: AATAGA"
     polyA_site      1217
                     /gene="Stap1"
                     /gene_synonym="Brdg1; STAP-1"
                     /note="major polyA site"
ORIGIN      
        1 gaacagttgc ctctcctctc aacactacgg agatttcggt ttttttgagg ctggatagaa
       61 ctgaaagcaa gccaaactcc aaagcaaagg ctatgatggc gaaaaagccc ccgaaacctg
      121 cccctcgcag gatcttccag gaaagactga agatcactgc tctgcccctg tactttgaag
      181 gatttttact ggtcaaacgg tctgatcacc aggagtacaa acactattgg acggagctga
      241 gagggaccac gctgttcttt tacactgaca aaaaaagcac aatctatgtt ggcaagttag
      301 atataataga cctcgtgtgc cttactggcc aacattcaac tgaaaagaat tgtgcaaaat
      361 tcactcttgt tttgcccaaa gaagaagtgc atgtgaagac agaaaacaca gaaagtggag
      421 aagaatggag gggcttcatt cttacagtaa cagagttgac agttcctcag catgtgtcac
      481 ttctacctgg gcaagtgatt agactgcacg aagtcttaga gagagaaaag aaaagaagga
      541 ttgagacaga tcagctgcct cttatgcctc cggagaaaga gaaggagcca gtacaagact
      601 atgcggatgt actgaaccca ctgcctgaat gtttttatgc agtttcccgg aaagaagcaa
      661 ctgcaatgct ggaaaagaac ccttcttggg gaaatatgat cctgaggcct ggtagtgaca
      721 gcaaaaacta ctccatcact atccggcagg agatagaaat gccaagaatc aagcacttca
      781 aagtgacgcg cactggaaac aactacacca tcgagctgga aaaacctgta acactcccaa
      841 accttttcag tgtcattgat tattttgtga aggagactcg agggaatttg agaccattta
      901 tacattcagc tgatgacaac tttggtcaag atcccaacat agaagacagg agtgagaagt
      961 ttaagaaaaa tccacacaat gcatgaagta acatgacacc tttcttcatt tatctcagta
     1021 tttattattt tcaaaaggaa gactttcact tttaaagaaa acaatctatt tccctgacct
     1081 agtttgggca taagaataaa atgatgtagc atataatttg attatcagca ggaaaataac
     1141 ttaccacaac tctactctgt agagtagctt gataacatgc atttctttgc attaaataga
     1201 aaaaaaaatt tactttccaa aacaa
//
