LOCUS       NM_009181               5354 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus ST8 alpha-N-acetyl-neuraminide
            alpha-2,8-sialyltransferase 2 (St8sia2), transcript variant 1,
            mRNA.
ACCESSION   NM_009181
VERSION     NM_009181.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Humpfle,L., Hachem,N.E., Simon,P., Weinhold,B., Galuska,S.P. and
            Middendorff,R.
  TITLE     Knockout of the polysialyltransferases ST8SiaII and ST8SiaIV leads
            to a dilatation of rete testis during postnatal development
  JOURNAL   Front Physiol 14, 1240296 (2023)
   PUBMED   37520830
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Shinde,P., Kiepas,A., Zhang,L., Sudhir,S., Konstantopoulos,K. and
            Stamatos,N.M.
  TITLE     Polysialylation controls immune function of myeloid cells in murine
            model of pneumococcal pneumonia
  JOURNAL   Cell Rep 42 (6), 112648 (2023)
   PUBMED   37339052
REFERENCE   3  (bases 1 to 5354)
  AUTHORS   Kucukerden,M., Schuster,U.E., Rockle,I., Alvarez-Bolado,G.,
            Schwabe,K. and Hildebrandt,H.
  TITLE     Compromised mammillary body connectivity and psychotic symptoms in
            mice with di- and mesencephalic ablation of ST8SIA2
  JOURNAL   Transl Psychiatry 12 (1), 51 (2022)
   PUBMED   35115485
  REMARK    GeneRIF: Compromised mammillary body connectivity and psychotic
            symptoms in mice with di- and mesencephalic ablation of ST8SIA2.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5354)
  AUTHORS   Delgado,C., Bu,L., Zhang,J., Liu,F.Y., Sall,J., Liang,F.X.,
            Furley,A.J. and Fishman,G.I.
  TITLE     Neural cell adhesion molecule is required for ventricular
            conduction system development
  JOURNAL   Development 148 (11) (2021)
   PUBMED   34100064
REFERENCE   5  (bases 1 to 5354)
  AUTHORS   Hachem,N.E., Humpfle,L., Simon,P., Kaese,M., Weinhold,B.,
            Gunther,J., Galuska,S.P. and Middendorff,R.
  TITLE     The Loss of Polysialic Acid Impairs the Contractile Phenotype of
            Peritubular Smooth Muscle Cells in the Postnatal Testis
  JOURNAL   Cells 10 (6), 1347 (2021)
   PUBMED   34072405
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5354)
  AUTHORS   Angata,K., Nakayama,J., Fredette,B., Chong,K., Ranscht,B. and
            Fukuda,M.
  TITLE     Human STX polysialyltransferase forms the embryonic form of the
            neural cell adhesion molecule. Tissue-specific expression, neurite
            outgrowth, and chromosomal localization in comparison with another
            polysialyltransferase, PST
  JOURNAL   J Biol Chem 272 (11), 7182-7190 (1997)
   PUBMED   9054414
REFERENCE   7  (bases 1 to 5354)
  AUTHORS   Yoshida,Y., Kurosawa,N., Kanematsu,T., Kojima,N. and Tsuji,S.
  TITLE     Genomic structure and promoter activity of the mouse polysialic
            acid synthase gene (mST8Sia II). Brain-specific expression from a
            TATA-less GC-rich sequence
  JOURNAL   J Biol Chem 271 (47), 30167-30173 (1996)
   PUBMED   8939967
REFERENCE   8  (bases 1 to 5354)
  AUTHORS   Yoshida,Y., Kojima,N., Kurosawa,N., Hamamoto,T. and Tsuji,S.
  TITLE     Molecular cloning of Sia alpha 2,3Gal beta 1,4GlcNAc alpha
            2,8-sialyltransferase from mouse brain
  JOURNAL   J Biol Chem 270 (24), 14628-14633 (1995)
   PUBMED   7782326
REFERENCE   9  (bases 1 to 5354)
  AUTHORS   Kojima,N., Yoshida,Y., Kurosawa,N., Lee,Y.C. and Tsuji,S.
  TITLE     Enzymatic activity of a developmentally regulated member of the
            sialyltransferase family (STX): evidence for alpha
            2,8-sialyltransferase activity toward N-linked oligosaccharides
  JOURNAL   FEBS Lett 360 (1), 1-4 (1995)
   PUBMED   7875291
REFERENCE   10 (bases 1 to 5354)
  AUTHORS   Kuhn,R., Lohler,J., Rennick,D., Rajewsky,K. and Muller,W.
  TITLE     Interleukin-10-deficient mice develop chronic enterocolitis
  JOURNAL   Cell 75 (2), 263-274 (1993)
   PUBMED   8402911
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC101658.8 and AC162361.7.
            
            On Mar 1, 2023 this sequence version replaced NM_009181.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC096546.1, X83562.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-264               AC101658.8         32072-32335
            265-327             AC101658.8         69012-69074
            328-456             AC101658.8         73686-73814
            457-714             AC101658.8         78734-78991
            715-1008            AC101658.8         84747-85040
            1009-1216           AC101658.8         102268-102475
            1217-5354           AC162361.7         1-4138
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 42.27 cM"
     gene            1..5354
                     /gene="St8sia2"
                     /gene_synonym="Siat8b; ST8SiaII; STX"
                     /note="ST8 alpha-N-acetyl-neuraminide
                     alpha-2,8-sialyltransferase 2"
                     /db_xref="GeneID:20450"
                     /db_xref="MGI:MGI:106020"
     exon            1..264
                     /gene="St8sia2"
                     /gene_synonym="Siat8b; ST8SiaII; STX"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..1294
                     /gene="St8sia2"
                     /gene_synonym="Siat8b; ST8SiaII; STX"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; sialyltransferase 8 (alpha-2,
                     8-sialyltransferase) B; alpha-2,8-sialyltransferase 8B;
                     SIAT8-B; sialyltransferase X; polysialic acid synthase;
                     sialytransferase St8Sia II; sialyltransferase St8Sia II"
                     /codon_start=1
                     /product="alpha-2,8-sialyltransferase 8B isoform 1
                     precursor"
                     /protein_id="NP_033207.2"
                     /db_xref="CCDS:CCDS21365.1"
                     /db_xref="GeneID:20450"
                     /db_xref="MGI:MGI:106020"
                     /translation="MQLQFRSWMLAALTLLVVFLIFADISEIEEEIGNSGGRGTIRSA
                     VNSLHSKSNRAEVVINGSSPPAVADRSNESLKHNIQPASSKWRHNQTLSLRIRKQILK
                     FLDAEKDISVLKGTLKPGDIIHYIFDRDSTMNVSQNLYELLPRTSPLKNKHFQTCAIV
                     GNSGVLLNSGCGQEIDTHSFVIRCNLAPVQEYARDVGLKTDLVTMNPSVIQRAFEDLV
                     NATWREKLLQRLHGLNGSILWIPAFMARGGKERVEWVNALILKHHVNVRTAYPSLRLL
                     HAVRGYWLTNKVHIKRPTTGLLMYTLATRFCNQIYLYGFWPFPLDQNQNPVKYHYYDS
                     LKYGYTSQASPHTMPLEFKALKSLHEQGALKLTVGQCDGAT"
     exon            265..327
                     /gene="St8sia2"
                     /gene_synonym="Siat8b; ST8SiaII; STX"
                     /inference="alignment:Splign:2.1.0"
     exon            328..456
                     /gene="St8sia2"
                     /gene_synonym="Siat8b; ST8SiaII; STX"
                     /inference="alignment:Splign:2.1.0"
     exon            457..714
                     /gene="St8sia2"
                     /gene_synonym="Siat8b; ST8SiaII; STX"
                     /inference="alignment:Splign:2.1.0"
     exon            715..1008
                     /gene="St8sia2"
                     /gene_synonym="Siat8b; ST8SiaII; STX"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..5354
                     /gene="St8sia2"
                     /gene_synonym="Siat8b; ST8SiaII; STX"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5333..5338
                     /regulatory_class="polyA_signal_sequence"
                     /gene="St8sia2"
                     /gene_synonym="Siat8b; ST8SiaII; STX"
                     /note="hexamer: AATAAA"
     polyA_site      5354
                     /gene="St8sia2"
                     /gene_synonym="Siat8b; ST8SiaII; STX"
                     /note="major polyA site"
ORIGIN      
        1 agagcaaccc ttgtctgttg ctgctgctgt cgccttggcc gccgccggcc aggactcgcc
       61 cggagcgcag ggtgtgtgcc ctgctgcgcg tggagcgcgg aggctccttc agccccagct
      121 cctcgccccg gcccgcgcgc gggtcccgga gggcaccgac cccaccatgc agctgcagtt
      181 ccggagctgg atgctggccg cgcttacgct gctcgtggtc ttcctcatct tcgcagacat
      241 ctcagagatc gaagaagaaa tcgggaattc tggaggcaga ggtacaatca gatcagctgt
      301 gaacagctta catagcaaat ctaatagagc tgaagttgta ataaatggct cttcaccgcc
      361 agctgtcgct gacagaagta atgaaagcct taagcacaac atccagccag cctcatccaa
      421 atggagacac aaccagacgc tctctctgag gatcaggaag caaattttaa agttcttgga
      481 tgcggagaag gatatttctg tccttaaggg gaccctgaag cctggagaca ttattcatta
      541 tatctttgat cgagacagca caatgaacgt gtcccagaac ctctatgaac tcctccccag
      601 aacctcacct ctgaagaata agcatttcca gacttgtgcc atcgtgggca actcgggggt
      661 cttgctgaac agcggctgtg gccaggagat tgacacacac agctttgtca taaggtgcaa
      721 cctggctcca gttcaggagt atgcccggga tgtgggcctc aagacagacc tagtgaccat
      781 gaacccctct gtcatccagc gggcctttga ggaccttgtg aacgccacgt ggcgggagaa
      841 gctgctgcag cggctgcacg gcctcaatgg gagcatcctg tggatccctg ccttcatggc
      901 ccggggtggc aaggagcgtg tggagtgggt caatgctctc atcctgaagc accatgtgaa
      961 cgtgcgcacg gcttaccctt ccctgcgcct gctgcacgca gtccgaggat attggctgac
     1021 caacaaagtc cacatcaaaa gaccaaccac cggcctcttg atgtacaccc tggccacacg
     1081 cttctgcaat cagatctacc tttatggctt ctggcccttc ccactggatc agaatcagaa
     1141 ccccgtcaag tatcactatt atgacagcct caagtatggc tacacttccc aggccagccc
     1201 ccacaccatg cccttggaat tcaaggccct caagagccta catgaacagg gggcattgaa
     1261 actgactgtc ggccagtgtg atggggctac gtaaggagga cagcccatgg gactcagtgg
     1321 ctcactttcc tgccagctac accacaggca gatgggagtc ggggtggcac aaacaataga
     1381 acaagcaggc tagtggtttt ctttgttaaa gtgtaaaaca gtgaccagaa tatatatatc
     1441 tatacctgca tatatatatt gacctgagta tttataactg tgtggtgttc atctagcact
     1501 aggcagataa gccacaggaa gaaggtgtgg agaacccacc tggaacagat ggagactcag
     1561 tccatctttg ggggcagaag gacttgacat gaaaagaagc cagtcccatg actttggatg
     1621 acaacccact tcctgggctg gatggatctt tgggctcatg gatgaatgga gagccacctg
     1681 ttgtcagctt ccccgaacct ccaagtgaag tggattctgg gtagcccaga cccagagatg
     1741 acagcttgga ccagtctaaa gaggtgcagt tcatggagga gagacttgga aaattccatt
     1801 gggcaaagag gcacaaggag agcaagcagc actgatcaca acggagctga gggctttgtt
     1861 catgaacttg agagtgcagc tgcccaaagg acaagtatgc atggcacctg cgtcccagac
     1921 aagccttcac agctatggag aaaggctgga ggaaactcag tgacagcccc atatcctggt
     1981 tagaccagta ccacccggga cttagtaggg cagaagagca catggcccta aagaggtaga
     2041 ttgttgcaaa tcgtggtttt agaaacttgg catcttctag gagtttgctc cacctcagaa
     2101 aaggacaaaa gggtgtacaa aataggatca ttttgttggg tgcagcccac ggaggcagaa
     2161 gtaaccctag gacttcaact tccaggttag cttttatccc atcctcttag aatgtcacat
     2221 gaaggaaact ctactgtccc atctactgcc agaaagacac agcaagtgct tgaaagagca
     2281 cagtcacaat aggtgtcatg ttgttcaaaa catatgcagt tttctaggca cctcacagag
     2341 ccctagaact ggacacctgg aagactgtga caaatctaac cctgggtctc tgcagtcact
     2401 ccctgtttgc tttctgcatc ctgcactctg gagtcgcctg ccgtgtcaat gtcaccttgt
     2461 cactttccca tcttgacagt catacctgcc cacaccaagc tcttctggga gaggttccag
     2521 tgtctaccct actacattcc tgcccaccct caccccccta ccccatcaac cattgcagag
     2581 tgtttgcaaa agccttcccc actctgttag gcctcccact taagatttgt ttttcttgta
     2641 aaaaagccca aagccaaaca cagttccaaa cactggacat aatctccggg ggaaatctgg
     2701 gctccatgcc aacaacttag gggttggact tacaggtcac acacagcaaa gcattagcca
     2761 gcttgatgtg ttagatgcag ttccctcgcc tgataaataa tggtgtttta aaatatattt
     2821 gaaagataaa tgtttaaaaa aaaaaacact attatcttga agatgaagag aggttttttt
     2881 tttaagtaat caagatctgc tcccaagcat atgaggcctg acgtaattaa tcaacagcca
     2941 ttcagaacac gatgcttgct ctaaattaat ggctgatggg aagtgagcag agaccatgga
     3001 gggataccct agaactatta gccctgcgta cattctctcc agccccaaac aggccaagga
     3061 ctgtacatct ctgttcccct ctttgttctt gttacagtat gaagcttgag atttgggatt
     3121 tgcatcataa aatgaatatt taaaaaagta acagccataa cagcctccct gggagctgag
     3181 tggctcagcc tcctcatgag cagtggagat aattagtatc atattttaca tgtctccaga
     3241 gcctttcatc tgaaggttct caaaggctct agaagacatt taatttctaa agtttgccgt
     3301 gccctggagg aataggtacg atcaccaaca gggcctcgaa ttagtaaaac ccttggatgt
     3361 cagaacatcc catgtggaat catgaagaag gcccagaggt caggttctgg aggtgaccct
     3421 gagccccacc cagcatcctc tctgtgaaga ggagttagag gggtaattgt ctaactagac
     3481 ttatgcaggc tgtaacctga tgggaaaggg cagaggtccc aggactaccc atgtatgccc
     3541 tttccctaac tcagcagccc ctgtgctgtt agaatggtct ggacatgtag ctgcagggtc
     3601 tctgtggacc ttggcttccc ctcttgccat tcttctccag tcagcagcca agtgtccttt
     3661 actgcctagt gagagcgcac ttggagctgg gctccactga agtcttttaa catgggctgg
     3721 ttgactttgg ctggccccag ggagacacag cctgcactgt taacctcata gctaccacac
     3781 aacatcacat cagcctcagc cagtcccctc aactgttggc tttcagtcac ctcctcagtc
     3841 tgcctgccta agcctgccag acatccattt actctaagcc cccagtgaca ccaccctcag
     3901 cctctgccat caccaaaaaa gaaaaagaaa aaaaaggggg gggggaccat ttggcatgag
     3961 actttatctc ataatctctt ggcctctccc tcccacttaa ccttcattct ttgacaaatg
     4021 aggtacctcc tgtaaatggg gagtctttcc tctcatgatc tcagactttt cccagccccc
     4081 cttgcattca ccctgattcc tatagcctct ccactcaact cctccactac cagaccttgg
     4141 cagggtctgc agtccctact gccaccagtt ccctgtacca caccttaatg tcccctctta
     4201 ccagcatctc aggcccctcc gtgtgctaaa tgcaaagccc tacagcaaat tcccctgggc
     4261 aagacactag gttcttaaga tttttatccc tcccatccca acctccatga ccatttattt
     4321 atatatttat ttatgtattt atgaggtatt tattggcatt cctaccttga gctgaaaaga
     4381 gccaaggaag atctgcccat ttcccctctc ttctccctgt accacaaagt agccattccc
     4441 tccaacaagg tcctccccaa aggcttataa gctgcgcatc cacagttcca tgccttcctt
     4501 cagactcaac tactacaacc caaagaatac ccttcactcc ccttagctgt ttagctccac
     4561 tggaagctcc cagttccagg atgatacact gcttttccag catactctat ctgctctgac
     4621 atcttgaact caagctcatc tgaagctaag tagaccatct ctcttccctg catgatccct
     4681 tattttctgg ttggaacacc aatgattctt tcaacacatt ctgggaccct aagatgatca
     4741 ctgactcttc catgtatccc ctcattaccc tttccaacta gtcccaacct cacctctcct
     4801 tgtcccttgg actccttagt agtggtctcc tctcccagac ctccctgagc atgcacgatg
     4861 gtattctcag ctcttcacga actcagctaa gatctcctga cccacagaaa tgtgcccatc
     4921 tttatcctga cattacactc aatcaggtcc cttcatagcc tgcactggcc aatccagaag
     4981 agttgtcagt attggggaaa gcaccacgtt tcctggccct gtgcctttgg ccttgcagtt
     5041 cgcatcttct ggatcattct tgagtttctt ccaaggcccc tgcagagacc acatctgcca
     5101 tgaagccctc cccgatccat caacgccggg aactctcccc ccacagaacc accacagcac
     5161 tttatttccc cctttctccc ggcacttacc atactctgcc ttgtattata gctccttgtg
     5221 cacttgtcct atgtctgttt gtagactgta accctgccaa gggcaggcac tgttttattc
     5281 atctgtatcc atcacagcat taggcgtggt gcatagcaca caacagttgc tcaataaatg
     5341 tttgttggat taaa
//
