LOCUS       NM_016973               2438 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus ST6
            (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,
            3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6
            (St6galnac6), transcript variant 1, mRNA.
ACCESSION   NM_016973
VERSION     NM_016973.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Taniguchi,M., Okumura,R., Matsuzaki,T., Nakatani,A., Sakaki,K.,
            Okamoto,S., Ishibashi,A., Tani,H., Horikiri,M., Kobayashi,N.,
            Yoshikawa,H.Y., Motooka,D., Okuzaki,D., Nakamura,S., Kida,T.,
            Kameyama,A. and Takeda,K.
  TITLE     Sialylation shapes mucus architecture inhibiting bacterial invasion
            in the colon
  JOURNAL   Mucosal Immunol 16 (5), 624-641 (2023)
   PUBMED   37385587
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Ngamukote,S., Yanagisawa,M., Ariga,T., Ando,S. and Yu,R.K.
  TITLE     Developmental changes of glycosphingolipids and expression of
            glycogenes in mouse brains
  JOURNAL   J Neurochem 103 (6), 2327-2341 (2007)
   PUBMED   17883393
REFERENCE   3  (bases 1 to 2438)
  AUTHORS   Tsuchida,A., Okajima,T., Furukawa,K., Ando,T., Ishida,H.,
            Yoshida,A., Nakamura,Y., Kannagi,R., Kiso,M. and Furukawa,K.
  TITLE     Synthesis of disialyl Lewis a (Le(a)) structure in colon cancer
            cell lines by a sialyltransferase, ST6GalNAc VI, responsible for
            the synthesis of alpha-series gangliosides
  JOURNAL   J Biol Chem 278 (25), 22787-22794 (2003)
   PUBMED   12668675
REFERENCE   4  (bases 1 to 2438)
  AUTHORS   Okajima,T., Chen,H.H., Ito,H., Kiso,M., Tai,T., Furukawa,K.,
            Urano,T. and Furukawa,K.
  TITLE     Molecular cloning and expression of mouse
            GD1alpha/GT1aalpha/GQ1balpha synthase (ST6GalNAc VI) gene
  JOURNAL   J Biol Chem 275 (10), 6717-6723 (2000)
   PUBMED   10702226
REFERENCE   5  (bases 1 to 2438)
  AUTHORS   Takashima,S., Kurosawa,N., Tachida,Y., Inoue,M. and Tsuji,S.
  TITLE     Comparative analysis of the genomic structures and promoter
            activities of mouse Siaalpha2,3Galbeta1,3GalNAc GalNAcalpha2,
            6-sialyltransferase genes (ST6GalNAc III and IV): characterization
            of their Sp1 binding sites
  JOURNAL   J Biochem 127 (3), 399-409 (2000)
   PUBMED   10731711
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY072844.1, AB035123.1, AK030648.1 and AK159158.1.
            
            On Jan 23, 2014 this sequence version replaced NM_016973.2.
            
            Transcript Variant: This variant (1) uses an alternate splice site
            in the 3' coding region, compared to variant 4. This results in a
            frameshift and an early stop codon, compared to variant 4. It
            encodes isoform 1, which is shorter and has a distinct C-terminus,
            compared to isoform 4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660813.214910.1,
                                           SRR1660817.993.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                BY072844.1         3-43
            42-1160             AB035123.1         3-1121
            1161-2433           AK030648.1         1074-2346
            2434-2438           AK159158.1         2416-2420
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 22.09 cM"
     gene            1..2438
                     /gene="St6galnac6"
                     /gene_synonym="Siat7f; ST6GalNAcVI"
                     /note="ST6
                     (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,
                     3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6"
                     /db_xref="GeneID:50935"
                     /db_xref="MGI:MGI:1355316"
     exon            1..127
                     /gene="St6galnac6"
                     /gene_synonym="Siat7f; ST6GalNAcVI"
                     /inference="alignment:Splign:2.1.0"
     exon            128..182
                     /gene="St6galnac6"
                     /gene_synonym="Siat7f; ST6GalNAcVI"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..1158
                     /gene="St6galnac6"
                     /gene_synonym="Siat7f; ST6GalNAcVI"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sialytransferase 7 ((alpha-N-acetylneuraminyl
                     2,3-betagalactosyl-1,3)-N-acetyl galactosaminide
                     alpha-2,6-sialytransferase) F;
                     alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase
                     6; (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,
                     3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6;
                     galNAc alpha-2,6-sialyltransferase VI"
                     /codon_start=1
                     /product="alpha-N-acetylgalactosaminide
                     alpha-2,6-sialyltransferase 6 isoform 1"
                     /protein_id="NP_058669.1"
                     /db_xref="CCDS:CCDS15923.1"
                     /db_xref="GeneID:50935"
                     /db_xref="MGI:MGI:1355316"
                     /translation="MACSRPPSQCDPTTLPPGPPAGRWPLPFSRRRREMSSNKEQRSA
                     VFVILFALITILILYSSNSANEVFHYGSLRGRTRRPVNLKKWSFSSAYFPILGNKTLP
                     SRCNQCVIITSSSHLLGTKLGPEIERAECTIRMNDAPTSGYSADVGNKTTFRVVAHSS
                     VFRVLRKPQEFVNRTPETVFIFWGPPNKMQKPQGSLLRVIQRAGLMFPNMEAYAVSPA
                     RMQQFDDLFRGETGKDREKSHSWLSTGWFTMVIAVELCDHVHVYGMVPPDYCSQRPRL
                     QRMPYHYYEPKGPDECVTYIQNEHSRKGNHHRFITEKRVFSSWAQLYGITFSHPSWT"
     exon            183..273
                     /gene="St6galnac6"
                     /gene_synonym="Siat7f; ST6GalNAcVI"
                     /inference="alignment:Splign:2.1.0"
     exon            274..453
                     /gene="St6galnac6"
                     /gene_synonym="Siat7f; ST6GalNAcVI"
                     /inference="alignment:Splign:2.1.0"
     exon            454..860
                     /gene="St6galnac6"
                     /gene_synonym="Siat7f; ST6GalNAcVI"
                     /inference="alignment:Splign:2.1.0"
     exon            861..968
                     /gene="St6galnac6"
                     /gene_synonym="Siat7f; ST6GalNAcVI"
                     /inference="alignment:Splign:2.1.0"
     exon            969..2438
                     /gene="St6galnac6"
                     /gene_synonym="Siat7f; ST6GalNAcVI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2415..2420
                     /regulatory_class="polyA_signal_sequence"
                     /gene="St6galnac6"
                     /gene_synonym="Siat7f; ST6GalNAcVI"
                     /note="hexamer: AATAAA"
     polyA_site      2435
                     /gene="St6galnac6"
                     /gene_synonym="Siat7f; ST6GalNAcVI"
                     /note="major polyA site"
ORIGIN      
        1 aggacccggc gggctggagc tgcggcgggg cctgggcggc cagagcggcg cggaccccgg
       61 ttctcggctc ccggcgccat gtgacggggt gcgggggccg cggggggccg ggcgctcccc
      121 ggcggagatg aggaaactga ggctcagaga ggtcacatgg cttgctcgag gccccccagc
      181 cagtgtgacc ccacaaccct gccccccggg ccacctgccg gacgctggcc tctacccttc
      241 agcagacgcc ggagagagat gagtagcaac aaagagcagc ggtcagcagt gtttgtgatc
      301 ctctttgccc tcatcaccat cctcatcctc tacagctcca acagtgccaa cgaggtcttc
      361 cactacggct ccctgcgggg ccgcacgcgt cggccagtca acctcaagaa gtggagtttc
      421 tccagcgcct acttccctat cctcggcaac aagacgctgc cgtccaggtg caaccagtgt
      481 gtgatcatca ccagctccag ccacctgctg ggcaccaaac tgggccctga gattgagcgg
      541 gctgagtgca ccatccgcat gaacgatgct cccacctctg gctactcggc tgacgtcgga
      601 aacaaaacca ccttccgcgt agtggcccat tccagtgtat tccgtgtgct gcggaagccc
      661 caggaatttg tcaaccggac ccctgagacg gtgttcatct tctggggacc cccaaacaag
      721 atgcagaagc cccagggcag cctcctgcgc gtcatccagc gggcgggcct catgttcccg
      781 aacatggagg cctatgccgt ctctcccgcc cgcatgcagc agtttgacga cctcttccgg
      841 ggtgagacgg gcaaggacag ggaaaagtcc cattcctggt tgagcacggg ctggtttacc
      901 atggtgattg cggtggaatt gtgtgaccat gtgcacgtgt atggcatggt ccctcctgac
      961 tactgcagcc agcggccccg cctgcagcgc atgccatacc actactatga acccaagggg
     1021 ccggacgagt gtgtcaccta catccagaac gagcacagcc gtaagggcaa tcaccaccgc
     1081 ttcatcaccg agaagagggt cttctcgtcc tgggcccaac tctacggtat caccttctcc
     1141 cacccctcct ggacctagtt atcctgtcca ccgatctcgg acagatacaa acgaagtggc
     1201 tctggcccag ccatttaacc atggccatct tccagccagt tcaggctggc tggagtattt
     1261 cccagccaat caaatcaggg ccttgatgag ggtttttttt ttcctccagc caatcggggc
     1321 tggggttatc tcttggccaa tcagggattt ggaattctgt atggtttaat cgggtgtcag
     1381 ggggtctttc tcgtctagtc agggtctgag catagtctat cagggtatag gggtctttct
     1441 gagtcaatct gaagcttagg acatgtcttt tcccataagg ctttgcttca gagccccagg
     1501 aatgggcccc caaatcactc cctccttagc tgggacaatg aggtcctgtc ctaaggatct
     1561 gaggacttgg tgttgcccca cagttgccag aatcagagct actgtagggg ggaggggctg
     1621 ccaggaggcc aggccaaagg atttgtggag ttctaggagt ggtagggaag cctaggaggt
     1681 aagaggcggt gtccagttcc cggtttcttc ctgagtgacc ttaaacagac cctcttgtct
     1741 ttctggtatg ctgtgatgac atcatagctc cgtatgtgga gaccctcact ctccgttata
     1801 cttaagtgta aattcctcag ctaccattcc tcgctggggg actcagctcc ctggggatgg
     1861 ggagcctggg gtacccttcc ctgcctcctt ctagaaactt tagggtattt ttgtgcaagt
     1921 gccccccatg gttgggggat cctgacagaa atggggcaaa catgaagctg tttctctagc
     1981 ccctcaatcc agctgccgtt agcctggctc tagaaaggcc aggcacctct tcctaccctc
     2041 cagcaggaga gcattccctg ctgcccgaag gagcctttgg gactgaggaa ggggtatccc
     2101 agccacacca gggcttcgtg aagtttttgt ctggagatgc tgagggcttt ggggagaagt
     2161 atcctccccc aagtttggga tgagactaga gatggggact gggttctgtc ccttgcttaa
     2221 gcactttatt aagggcagga ctgggtgggg gtgtgggtaa cccccattct tccacagcct
     2281 gcaagggaag ggcttccaga aaggctgtgg actgggggtt gggggagact agccagcagt
     2341 gagccctgca cagcaaggta atgggggttg ggcagtgaca gccctagatc tggttttgta
     2401 atgatttata cagaaataaa cacgcctaag cccaactc
//
