LOCUS       NP_001342590             615 aa            linear   ROD 02-MAY-2024
DEFINITION  afadin- and alpha-actinin-binding protein isoform a [Mus musculus].
ACCESSION   NP_001342590 XP_006502467
VERSION     NP_001342590.1
DBSOURCE    REFSEQ: accession NM_001355661.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 615)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 615)
  AUTHORS   Wang,B., He,W., Prosseda,P.P., Li,L., Kowal,T.J., Alvarado,J.A.,
            Wang,Q., Hu,Y. and Sun,Y.
  TITLE     OCRL regulates lysosome positioning and mTORC1 activity through
            SSX2IP-mediated microtubule anchoring
  JOURNAL   EMBO Rep 22 (7), e52173 (2021)
   PUBMED   33987909
  REMARK    GeneRIF: OCRL regulates lysosome positioning and mTORC1 activity
            through SSX2IP-mediated microtubule anchoring.
REFERENCE   3  (residues 1 to 615)
  AUTHORS   Kurtulmus,B., Wang,W., Ruppert,T., Neuner,A., Cerikan,B., Viol,L.,
            Duenas-Sanchez,R., Gruss,O.J. and Pereira,G.
  TITLE     WDR8 is a centriolar satellite and centriole-associated protein
            that promotes ciliary vesicle docking during ciliogenesis
  JOURNAL   J Cell Sci 129 (3), 621-636 (2016)
   PUBMED   26675238
REFERENCE   4  (residues 1 to 615)
  AUTHORS   Klinger,M., Wang,W., Kuhns,S., Barenz,F., Drager-Meurer,S.,
            Pereira,G. and Gruss,O.J.
  TITLE     The novel centriolar satellite protein SSX2IP targets Cep290 to the
            ciliary transition zone
  JOURNAL   Mol Biol Cell 25 (4), 495-507 (2014)
   PUBMED   24356449
  REMARK    GeneRIF: The novel centriolar satellite protein SSX2IP targets
            Cep290 to the ciliary transition zone.
REFERENCE   5  (residues 1 to 615)
  AUTHORS   Fukumoto,Y., Kurita,S., Takai,Y. and Ogita,H.
  TITLE     Role of scaffold protein afadin dilute domain-interacting protein
            (ADIP) in platelet-derived growth factor-induced cell movement by
            activating Rac protein through Vav2 protein
  JOURNAL   J Biol Chem 286 (50), 43537-43548 (2011)
   PUBMED   22027834
  REMARK    GeneRIF: The results indicate that ADIP plays an essential role in
            PDGF-induced cell movement by interacting with afadin and Vav2 and
            regulating the activation of Rac.
REFERENCE   6  (residues 1 to 615)
  AUTHORS   Asada,M., Irie,K., Yamada,A. and Takai,Y.
  TITLE     Afadin- and alpha-actinin-binding protein ADIP directly binds
            beta'-COP, a subunit of the coatomer complex
  JOURNAL   Biochem Biophys Res Commun 321 (2), 350-354 (2004)
   PUBMED   15358183
REFERENCE   7  (residues 1 to 615)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   8  (residues 1 to 615)
  AUTHORS   Asada,M., Irie,K., Morimoto,K., Yamada,A., Ikeda,W., Takeuchi,M.
            and Takai,Y.
  TITLE     ADIP, a novel Afadin- and alpha-actinin-binding protein localized
            at cell-cell adherens junctions
  JOURNAL   J Biol Chem 278 (6), 4103-4111 (2003)
   PUBMED   12446711
  REMARK    GeneRIF: localization at cell-cell adherens junctions
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124987.9.
            
            On Sep 22, 2017 this sequence version replaced XP_006502467.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7974084.29219.1,
                                           SRR7652917.576055.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 71.03 cM"
     Protein         1..615
                     /product="afadin- and alpha-actinin-binding protein
                     isoform a"
                     /note="afadin DIL domain-interacting protein; afadin- and
                     alpha-actinin-binding protein; synovial sarcoma, X
                     breakpoint 2 interacting protein; synovial sarcoma, X 2
                     interacting protein; SSX 2 interacting protein"
                     /calculated_mol_wt=70825
     Region          <63..392
                     /region_name="SCP-1"
                     /note="Synaptonemal complex protein 1 (SCP-1); pfam05483"
                     /db_xref="CDD:114219"
     Region          63..214
                     /region_name="ADIP"
                     /note="Afadin- and alpha -actinin-Binding; pfam11559"
                     /db_xref="CDD:463295"
     Site            290
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y2D8; propagated from
                     UniProtKB/Swiss-Prot (Q8VC66.1)"
     Region          293..316
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8VC66.1)"
     Site            293
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y2D8; propagated from
                     UniProtKB/Swiss-Prot (Q8VC66.1)"
     Site            313
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8VC66.1)"
     Site            319
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8VC66.1)"
     Site            537
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y2D8; propagated from
                     UniProtKB/Swiss-Prot (Q8VC66.1)"
     Site            541
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y2D8; propagated from
                     UniProtKB/Swiss-Prot (Q8VC66.1)"
     Site            543
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y2D8; propagated from
                     UniProtKB/Swiss-Prot (Q8VC66.1)"
     Region          567..615
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8VC66.1)"
     CDS             1..615
                     /gene="Ssx2ip"
                     /gene_synonym="Adip"
                     /coded_by="NM_001355661.1:436..2283"
                     /note="isoform a is encoded by transcript variant 5"
                     /db_xref="CCDS:CCDS38665.1"
                     /db_xref="GeneID:99167"
                     /db_xref="MGI:MGI:2139150"
ORIGIN      
        1 mgdwmtvtdp vlctenknls qytsetkmsp sslysqqvlc ssvplsknvh gvfgvfctge
       61 nieqsisyld qelttfgfps lyeeskskea krelnivavl ncmnellvlq rknllaqesv
      121 etqnlklgsd mdhlqscyak lkeqletsrr emiglqerdr qlqcknrslh qllknekdev
      181 qklqniiasr atqynhdvkr kereynklke rlhqlvmnkk dkniamdvln yvgradgkrg
      241 swrtdktear nedemykill ndyeyrqkqi lmenaelkkv lqqmkkemis llspqkkkpr
      301 eraedgtgtv aisdieddsg elsrdsvwgl scdtvreqlt nsirkqwril kshvekldnq
      361 askvhsegln eedvisrqdh eqeteklele ierckemika qqqllqqqla ttcdddttsl
      421 lrdcylleek erlkeewtlf keqkknfere rrsfteaair lglerkafee eraswvkqqf
      481 lnmtnfdhqn senvklfsaf sgssdpdnli vhsrprqkkl hsvangvpac tskltkslpa
      541 spstsdfrqt hscvsehssi svlnitpees kpsevarest dqkwsvqsrp ssregcysgc
      601 ssafrsahgd rddlp
//
