LOCUS       NM_010656               4433 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus sarcospan (Sspn), transcript variant 1, mRNA.
ACCESSION   NM_010656
VERSION     NM_010656.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Hwang,H.S., Kahmini,A.R., Prascak,J., Cejas-Carbonell,A.,
            Valera,I.C., Champion,S., Corrigan,M., Mumbi,F. and Parvatiyar,M.S.
  TITLE     Sarcospan Deficiency Increases Oxidative Stress and Arrhythmias in
            Hearts after Acute Ischemia-Reperfusion Injury
  JOURNAL   Int J Mol Sci 24 (14), 11868 (2023)
   PUBMED   37511627
  REMARK    GeneRIF: Sarcospan Deficiency Increases Oxidative Stress and
            Arrhythmias in Hearts after Acute Ischemia-Reperfusion Injury.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4433)
  AUTHORS   Gibbs,E.M., McCourt,J.L., Shin,K.M., Hammond,K.G., Marshall,J.L.
            and Crosbie,R.H.
  TITLE     Loss of sarcospan exacerbates pathology in mdx mice, but does not
            affect utrophin amelioration of disease
  JOURNAL   Hum Mol Genet 30 (3-4), 149-159 (2021)
   PUBMED   33432327
  REMARK    GeneRIF: Loss of sarcospan exacerbates pathology in mdx mice, but
            does not affect utrophin amelioration of disease.
REFERENCE   4  (bases 1 to 4433)
  AUTHORS   Peter,A.K., Miller,G., Capote,J., DiFranco,M., Solares-Perez,A.,
            Wang,E.L., Heighway,J., Coral-Vazquez,R.M., Vergara,J. and
            Crosbie-Watson,R.H.
  TITLE     Nanospan, an alternatively spliced isoform of sarcospan, localizes
            to the sarcoplasmic reticulum in skeletal muscle and is absent in
            limb girdle muscular dystrophy 2F
  JOURNAL   Skelet Muscle 7 (1), 11 (2017)
   PUBMED   28587652
  REMARK    GeneRIF: An alternatively spliced isoform of sarcospan localizes to
            the sarcoplasmic reticulum in skeletal muscle.  It is absent in
            Sgcd-/- skeletal muscle, a murine model for limb girdle muscular
            dystrophy 2F.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4433)
  AUTHORS   Gibbs,E.M., Marshall,J.L., Ma,E., Nguyen,T.M., Hong,G., Lam,J.S.,
            Spencer,M.J. and Crosbie-Watson,R.H.
  TITLE     High levels of sarcospan are well tolerated and act as a
            sarcolemmal stabilizer to address skeletal muscle and pulmonary
            dysfunction in DMD
  JOURNAL   Hum Mol Genet 25 (24), 5395-5406 (2016)
   PUBMED   27798107
  REMARK    GeneRIF: these results demonstrate that SSPN overexpression is well
            tolerated in mdx mice and improves sarcolemma defects that underlie
            skeletal muscle and pulmonary dysfunction in Duchenne muscular
            dystrophy
REFERENCE   6  (bases 1 to 4433)
  AUTHORS   Manenti,G., Stafford,A., De Gregorio,L., Gariboldi,M.,
            Falvella,F.S., Avner,P. and Dragani,T.A.
  TITLE     Linkage disequilibrium and physical mapping of Pas1 in mice
  JOURNAL   Genome Res 9 (7), 639-646 (1999)
   PUBMED   10413402
REFERENCE   7  (bases 1 to 4433)
  AUTHORS   Crosbie,R.H., Lebakken,C.S., Holt,K.H., Venzke,D.P., Straub,V.,
            Lee,J.C., Grady,R.M., Chamberlain,J.S., Sanes,J.R. and
            Campbell,K.P.
  TITLE     Membrane targeting and stabilization of sarcospan is mediated by
            the sarcoglycan subcomplex
  JOURNAL   J Cell Biol 145 (1), 153-165 (1999)
   PUBMED   10189375
REFERENCE   8  (bases 1 to 4433)
  AUTHORS   Heighway,J., Betticher,D.C., Hoban,P.R., Altermatt,H.J. and
            Cowen,R.
  TITLE     Coamplification in tumors of KRAS2, type 2 inositol 1,4,5
            triphosphate receptor gene, and a novel human gene, KRAG
  JOURNAL   Genomics 35 (1), 207-214 (1996)
   PUBMED   8661122
REFERENCE   9  (bases 1 to 4433)
  AUTHORS   Scott,A.F., Elizaga,A., Morrell,J., Bergen,A. and Penno,M.B.
  TITLE     Characterization of a gene coamplified with Ki-ras in Y1 murine
            adrenal carcinoma cells that codes for a putative membrane protein
  JOURNAL   Genomics 20 (2), 227-230 (1994)
   PUBMED   8020969
REFERENCE   10 (bases 1 to 4433)
  AUTHORS   George,D.L., Scott,A.F., de Martinville,B. and Francke,U.
  TITLE     Amplified DNA in Y1 mouse adrenal tumor cells: isolation of cDNAs
            complementary to an amplified c-Ki-ras gene and localization of
            homologous sequences to mouse chromosome 6
  JOURNAL   Nucleic Acids Res 12 (6), 2731-2743 (1984)
   PUBMED   6546797
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC144936.4.
            
            On Mar 19, 2018 this sequence version replaced NM_010656.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U02487.1, AK162864.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-250               AC144936.4         62012-62261         c
            251-337             AC144936.4         40702-40788         c
            338-4433            AC144936.4         31158-35253         c
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 77.7 cM"
     gene            1..4433
                     /gene="Sspn"
                     /gene_synonym="Gm32675; Krag"
                     /note="sarcospan"
                     /db_xref="GeneID:16651"
                     /db_xref="MGI:MGI:1353511"
     exon            1..250
                     /gene="Sspn"
                     /gene_synonym="Gm32675; Krag"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..703
                     /gene="Sspn"
                     /gene_synonym="Gm32675; Krag"
                     /note="isoform 1 is encoded by transcript variant 1;
                     kirsten-Ras-associated protein; K-ras oncogene-associated
                     protein; Kras oncogene-associated"
                     /codon_start=1
                     /product="sarcospan isoform 1"
                     /protein_id="NP_034786.1"
                     /db_xref="CCDS:CCDS20698.1"
                     /db_xref="GeneID:16651"
                     /db_xref="MGI:MGI:1353511"
                     /translation="MGRKPSPRAQELPEEEARTCCGCRFPLLLALLQLALGIAVTVLG
                     FLMASISPSLLVRDTPFWAGIIVCVVAYLGLFMLCVSYQVDERTCVQFSMKVFYFLLS
                     ALGLMVCMLAVAFAAHHYSLLAQFTCETSLDSCQCKLPSSEPLSRAFVYRDVTDCTSV
                     TGTFKLFLIIQMVLNLVCGLVCLLACFVMWKHRYQVFYVGVGLRSLMASDGQLPKA"
     exon            251..337
                     /gene="Sspn"
                     /gene_synonym="Gm32675; Krag"
                     /inference="alignment:Splign:2.1.0"
     exon            338..4433
                     /gene="Sspn"
                     /gene_synonym="Gm32675; Krag"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4408..4413
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sspn"
                     /gene_synonym="Gm32675; Krag"
                     /note="hexamer: AATAAA"
     polyA_site      4431
                     /gene="Sspn"
                     /gene_synonym="Gm32675; Krag"
                     /note="major polyA site"
ORIGIN      
        1 agactgggca ggcccagcag caaggcccag gcgcaggcac caccgaggga acatggggcg
       61 caagccgagc cccagggctc aggagctgcc tgaggaggag gcgcggacct gctgtggctg
      121 ccgcttcccg ctgctgctgg cactgctgca gctggcgttg ggcatcgcgg tgaccgtgct
      181 gggcttcctc atggcgagca tcagcccctc cctgctagtc agagatactc cgttctgggc
      241 tgggatcatt gtctgcgtgg tggcttacct tggactgttc atgctttgtg tctcatacca
      301 agttgacgag aggacatgtg tccagttttc tatgaaggta ttctacttcc tgttgagtgc
      361 cctcggcctg atggtctgca tgctggctgt ggcctttgcc gcccaccact actccctgct
      421 tgcgcagttt acctgcgaga cctccctgga ttcttgccag tgcaagctgc cttcctcgga
      481 gccgctcagc agggcctttg tttacaggga tgtgactgac tgtaccagcg tcactggcac
      541 tttcaaattg ttcttaatca tccagatggt cctaaacctg gtctgtggcc tcgtgtgctt
      601 gctggcttgc tttgtgatgt ggaaacacag gtaccaggtc ttttatgtgg gtgttgggct
      661 gcgctccctg atggcttctg atggccagct accaaaggcc taacaggttg gctcagaggg
      721 agacaagctc agaaacggca cacccactga ctaggcaagg tgaaaaaaac cacaattttg
      781 taaaggcaaa cttttgacaa cgaatacttt ttcattctct aaatgtgtat ttttagattt
      841 ttttttcaga aaacaaaaca acttgttttt tggatctcta ttgtactttt taaaaacttc
      901 ttgtgaggac aggggtggag agacggctta caagtgaaga ggacttgctg ctcttgcgga
      961 ggactcgggt tcaagctcag ttccggagcc caagttcagt ggctcacagt tacctgtacc
     1021 tccagctccg gggacattgg gagcacacgc atgcgcacac gcgtgattta gagaatctaa
     1081 cctattttta aagttctaat gagaaacaag attcaaattg acttggtgaa caccgaaagg
     1141 aaaagaacac cagacaggga agagatgacc tagagccagc gctagctctg gtgtttatcc
     1201 cagccgccct tacatcctca ccctcctccg ccttcccgtt agtccagtgc gatggtgtga
     1261 actggcatca cccatagact ctagagtctt ccggctcctc taccacctgc cattatcccc
     1321 gtggtgtctt gggaaaggtc taaggaaagc tggatctcag ttgagtgctt ttatgttttc
     1381 acagtggaga tgtcttcaag gcatgaaatg gctttaaaaa aaaaaaacta gttgaacagg
     1441 aaggctgagt gattctgaac acctgggctg gggtgcagac ttccggttgc cttagctccg
     1501 atccacgagt gggctgcagc ctccgtgagg tctgattcac cataattgaa aggtggtgct
     1561 gttgcattct taacgacgag gaaagtttaa aaataattat attatgcctc tagtctgtca
     1621 tctaaaacaa atcattaaga ctaatttcca gctaattgtt cattataatt atgctcagga
     1681 ttctgtttaa tgagctctgg ctgttcttag gcagcaccgt tggtgttagg aaaaattaca
     1741 gccacaaggg aagatggctg aagatccact ttcctggaaa tcatccactg caaaaaatga
     1801 tcttgtaaaa cattgaaaca ctctcagagg ggaaacaacc aagagcattt gtcacaggaa
     1861 atgcttatat taacaacaca tttgtgatat tgaaaagtat ctcccaggac caccaagtac
     1921 ctttgatggg attgggtact aagtcctgtc atacacatgg acttcctggg aaatcctagt
     1981 aatgagggct tttacaattg acctagagac agaatgtctc aaagcccttg tttgggtgat
     2041 acgtccacag ttagggtatt cagtctcagt ttagagttaa gacatggtac ttggttaaga
     2101 cagactttgc tatgtaattg ggtaaaattc caccaaaagg aatggggtgg tggtggcaca
     2161 tgtctttaat tccggcactt gggaagtaga ggcaggtgta tttctgtgag ttgaaggcta
     2221 gcctggtcta caaagaaagt tctaggacag ccagagcaga gagagaaacc ctgtcttgaa
     2281 aaaaacaaaa cttaacaaac aaaaacaaga aaaaaaaagg gggggggact gctttcagac
     2341 aaaattgcat gaatgccaca gtaagcttcc tggtactaac ctgggaaacc tttagtgttt
     2401 gttaagaata accagctccg ggctgtggag atggctcagt tatcaaagtc cttgccatcc
     2461 cagtaagaac ctgatcccat gtaaaacagc caggcacagg tgtgtgcttg tgatcctggc
     2521 attgcagagg cagagacagg cagatccctg gggctccttc accagccatc tttagtgtag
     2581 tgattaactt caggtccatg atgagagagt ttgtctcaag aaaaaaacca acactaaaaa
     2641 taacccggag tggatagctg gcccctggcc ttcccatgaa tgcacacaca cagttaagag
     2701 taaccaatct cacgctgact ttcgatagtt ttctataaaa catggtacat ggtagagaat
     2761 ccaggatgtc acgggctgtc ctttcaaagt gacgctgttt tcacgcctgt tactgtgcac
     2821 ccagccctgc tggaagcaca cacagaatgc ctttgtccaa caaactcaac aggtttttgt
     2881 ttttgttttt taaatgagga aattaagatt cccccacaca gttaagttgc tctaagcttt
     2941 acaaacgtat gctttacaaa caactcacgg gggggggggg cattttaaag ggtagagcaa
     3001 ttttagcctg tgggtttctt acttttctaa cacaaccaag gcttggggtg gttccagctt
     3061 ataatattct gtctgcataa gatggggatt ttactattat gatcaaatta ttaacttccc
     3121 aaagaggagg aggcactgta agtgaagaga aggagcagag ccgggtggca ggaccctgtg
     3181 ctgggagctt acgatatgct ggtgtagtaa gggctacgct gcgtgagtaa caaatgactt
     3241 tgggccggat ctgaaatatt ttgacgttat gttttgcaaa tattgaagct tcagtaaggg
     3301 cagcagagaa ggagcgggca cacagcttct cacttagcaa aatgtcacac ttcaaatctg
     3361 tcacagagcc aggacggaac ggtgactgtg ccttgtgcct tccaaactgt agaacggaga
     3421 ggtttgcaaa gcccttcctc ccccagcctc cttcaggaga attaaatgaa tcaagacttt
     3481 tcaaagagga gaaacggcca gggcttggca gtacttggaa taggaatacg gccacctggg
     3541 tgttcgggct gtaactgagc ccactcaacg ggtctgtgtc tttatgtgac ctttgtggct
     3601 ccgagctcca tccaaattgg tataggcagc atcttggagc catgcctaaa ccgtagctac
     3661 tccaaagccc tagaaactgt agtcaagtaa caggcctcac gccccgtctt tggattgaga
     3721 gctcagatct tctcttctga gtgccttcca gtgtgttcct acagcgacac tccattctat
     3781 atgtttttct attcacatcc aagacatgag gatctcttga tgctccctca cttctgtgtg
     3841 cattatatct ggggcatatt tatgaaattg tagtagagtc ttcattaaag ccatcaaagg
     3901 atattaggag gggcaactat agaggggcca ctgaaagatg attagaaata accttgaaag
     3961 ctgtccacca tttgcagtca tgaacggtgt cttcatattt cctggtgact acctcagaac
     4021 tgacactgcg tgatcacctt tgtataagtg tgccgcagat atagcgggtt cacctttgta
     4081 taagtgtgcc gcagatatag cgggttcacc tttgtataag tgtgccgcag atatagcggg
     4141 tttactctcg ttaccccaga atgtgtttta atggtacagt gcaagttcag cctctctgat
     4201 ggccagctgc ttagtgtgta agccactgaa atggattcgt gtcttctcat tttcttgtct
     4261 caagtgagca ctgcccctct cagaattatg agtctgcata tggtgctgca gccgcctttc
     4321 ctggccttga tcaaattgag agtagacgat ggagtgtttc tacacatttg aggtggttat
     4381 ttttaaaggg tgtgtgtttc ctttatcaat aaacttttca taattcacaa aga
//
