LOCUS       NM_198109               8332 bp    mRNA    linear   ROD 18-JUN-2024
DEFINITION  Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript
            variant 1, mRNA.
ACCESSION   NM_198109 XM_357561
VERSION     NM_198109.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   Lai,S.W., Cheng,Y.C., Huang,W.C., Yadav,V.K., Fong,I.H., Yeh,C.T.,
            Yang,C.K., Lee,W.H. and Chen,M.Y.
  TITLE     Dysregulated expression of slingshot protein phosphatase 1 (SSH1)
            disrupts circadian rhythm and WNT signaling associated to
            hepatocellular carcinoma pathogenesis
  JOURNAL   Aging (Albany NY) 15 (20), 11033-11051 (2023)
   PUBMED   37837551
  REMARK    GeneRIF: Dysregulated expression of slingshot protein phosphatase 1
            (SSH1) disrupts circadian rhythm and WNT signaling associated to
            hepatocellular carcinoma pathogenesis.
REFERENCE   3  (bases 1 to 8332)
  AUTHORS   Cazzaro,S., Woo,J.A., Wang,X., Liu,T., Rego,S., Kee,T.R., Koh,Y.,
            Vazquez-Rosa,E., Pieper,A.A. and Kang,D.E.
  TITLE     Slingshot homolog-1-mediated Nrf2 sequestration tips the balance
            from neuroprotection to neurodegeneration in Alzheimer's disease
  JOURNAL   Proc Natl Acad Sci U S A 120 (30), e2217128120 (2023)
   PUBMED   37463212
REFERENCE   4  (bases 1 to 8332)
  AUTHORS   Cazzaro,S., Fang,C., Khan,H., Witas,R., Kee,T.R., Woo,J.A. and
            Kang,D.E.
  TITLE     Slingshot homolog-1 mediates the secretion of small extracellular
            vesicles containing misfolded proteins by regulating autophagy
            cargo receptors and actin dynamics
  JOURNAL   Front Aging Neurosci 14, 933979 (2022)
   PUBMED   36092812
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8332)
  AUTHORS   Fang,C., Woo,J.A., Liu,T., Zhao,X., Cazzaro,S., Yan,Y., Matlack,J.,
            Kee,T., LePochat,P. and Kang,D.E.
  TITLE     SSH1 impedes SQSTM1/p62 flux and MAPT/Tau clearance independent of
            CFL (cofilin) activation
  JOURNAL   Autophagy 17 (9), 2144-2165 (2021)
   PUBMED   33044112
REFERENCE   6  (bases 1 to 8332)
  AUTHORS   Maheswaranathan,M., Gole,H.K.A., Fernandez,I., Lassegue,B.,
            Griendling,K.K. and San Martin,A.
  TITLE     Platelet-derived growth factor (PDGF) regulates Slingshot
            phosphatase activity via Nox1-dependent auto-dephosphorylation of
            serine 834 in vascular smooth muscle cells
  JOURNAL   J Biol Chem 286 (41), 35430-35437 (2011)
   PUBMED   21857021
  REMARK    GeneRIF: PDGF activates SSH1L in VSMC by a mechanism that involves
            Nox1-mediated oxidation of 14-3-3 and Ser-834 SSH1L
            auto-dephosphorylation.
            Erratum:[J Biol Chem. 2020 Jun 12;295(24):8348. doi:
            10.1074/jbc.AAC120.014380. PMID: 32532854]
REFERENCE   7  (bases 1 to 8332)
  AUTHORS   Huang,T.Y., Minamide,L.S., Bamburg,J.R. and Bokoch,G.M.
  TITLE     Chronophin mediates an ATP-sensing mechanism for cofilin
            dephosphorylation and neuronal cofilin-actin rod formation
  JOURNAL   Dev Cell 15 (5), 691-703 (2008)
   PUBMED   19000834
REFERENCE   8  (bases 1 to 8332)
  AUTHORS   Kurita,S., Gunji,E., Ohashi,K. and Mizuno,K.
  TITLE     Actin filaments-stabilizing and -bundling activities of
            cofilin-phosphatase Slingshot-1
  JOURNAL   Genes Cells 12 (5), 663-676 (2007)
   PUBMED   17535256
  REMARK    GeneRIF: Knockdown of SSH1 expression by RNA interference
            significantly suppressed stress fiber formation in C2C12 myoblast
            cells, indicating a role for SSH1 in stress fiber formation or
            stabilization in cells.
REFERENCE   9  (bases 1 to 8332)
  AUTHORS   Soosairajah,J., Maiti,S., Wiggan,O., Sarmiere,P., Moussi,N.,
            Sarcevic,B., Sampath,R., Bamburg,J.R. and Bernard,O.
  TITLE     Interplay between components of a novel LIM kinase-slingshot
            phosphatase complex regulates cofilin
  JOURNAL   EMBO J 24 (3), 473-486 (2005)
   PUBMED   15660133
REFERENCE   10 (bases 1 to 8332)
  AUTHORS   Ohta,Y., Kousaka,K., Nagata-Ohashi,K., Ohashi,K., Muramoto,A.,
            Shima,Y., Niwa,R., Uemura,T. and Mizuno,K.
  TITLE     Differential activities, subcellular distribution and tissue
            expression patterns of three members of Slingshot family
            phosphatases that dephosphorylate cofilin
  JOURNAL   Genes Cells 8 (10), 811-824 (2003)
   PUBMED   14531860
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC145559.4.
            
            On May 19, 2018 this sequence version replaced NM_198109.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK155557.1, AK171556.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-127               AC145559.4         169568-169694       c
            128-168             AC145559.4         165550-165590       c
            169-272             AC145559.4         146275-146378       c
            273-337             AC145559.4         142613-142677       c
            338-459             AC145559.4         142084-142205       c
            460-528             AC145559.4         137254-137322       c
            529-594             AC145559.4         136504-136569       c
            595-789             AC145559.4         134618-134812       c
            790-883             AC145559.4         133288-133381       c
            884-1012            AC145559.4         131622-131750       c
            1013-1059           AC145559.4         129157-129203       c
            1060-1206           AC145559.4         127783-127929       c
            1207-1407           AC145559.4         126290-126490       c
            1408-1960           AC145559.4         122180-122732       c
            1961-8332           AC145559.4         112998-119369       c
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 55.82 cM"
     gene            1..8332
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /note="slingshot protein phosphatase 1"
                     /db_xref="GeneID:231637"
                     /db_xref="MGI:MGI:2686240"
     exon            1..127
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     exon            128..168
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     exon            169..272
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     exon            273..337
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     exon            338..459
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     exon            460..528
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     exon            529..594
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     exon            595..789
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     exon            790..883
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     exon            884..1012
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1059
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1206
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1407
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1960
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..8332
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3728..3733
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /note="hexamer: TATAAA"
     polyA_site      3748
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /note="major polyA site"
     regulatory      8309..8314
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /note="hexamer: AATAAA"
     polyA_site      8329
                     /gene="Ssh1"
                     /gene_synonym="Gm1394; Gm1395; SSH-1; SSH-1L"
                     /note="major polyA site"
ORIGIN      
        1 gggcccggcg cggctgcccg gccggctgct ggaggccccg ggccgggccc gcgccgcgat
       61 ggccttggtg acccttcagc gctctcccac gcccagcgcc gcgtcttcct ccgcaagcaa
      121 cagcgagttg gaggctggca gcgatgaaga acggaaattg aacctcagct tgagtgagag
      181 ctttttcatg gtgaaaggag ctgccctctt cctacagcag ggaaacagcc cacagggcca
      241 gcggagtctt cagcaccctc acaagcatgc aggtgatctg cctcagcacc tgcaagtgat
      301 gatcaacctc ctgcgttgtg aagacagaat caagctggcc gtgcgcctag agagtgtctg
      361 gaccgaccgt gtccgctaca tggtcgtggt atacaccagc gggcgccagg acaccgagga
      421 gaatattctg ctgggagttg acttttccag taaggagagc aaaagctgca cgatcggaat
      481 ggttcttcga ctctggagcg acaccaagat tcacctcgat ggggacggcg ggttcagtgt
      541 gagcacagcg ggcaggatgc acatattcaa gccagtgtct gtccaggcca tgtggtctgc
      601 cctgcaagtg cttcacaagg cctgcgaagt ggcccggagg cataactact tcccaggagg
      661 agtggcgctc atctgggcca cctactatga gagctgcatc agctcggagc agagctgcat
      721 caatgagtgg aatgccatgc aggacctgga gtccacgcgg cccgactccc ccgcgctgtt
      781 tgtggacaag ccaaccgagg gcgaaagaac tgagcgcctc attaaagcca aactccggag
      841 catcatgatg agccaggacc ttgaaaatgt gacttctaag gaaatccgaa atgagctgga
      901 gaagcaaatg aactgcaacc tgaaggagtt caaggaattc atcgataacg agatgctgct
      961 catcctgggc cagatggaca agccctccct catctttgac catctttatc ttggctccga
     1021 gtggaatgca tccaatctgg aggagctgca gggctcagga gttgactaca ttttaaatgt
     1081 cactagagaa atagacaatt ttttccccgg cttgtttgcg taccataaca tccgcgtgta
     1141 cgatgaggag accacagacc ttcttgccca ctggaatgaa gcttatcatt tcataaacaa
     1201 agcgaaaagg aatcattcca agtgcctggt ccattgcaag atgggcgtca gccgatctgc
     1261 gtccacagtc attgcctacg ccatgaagga gttcggctgg cccctggaga aagcgtataa
     1321 ctacgtgaag cagaaacgta gtatcactcg gcccaacgca ggctttatga ggcagctgtc
     1381 tgagtacgaa ggcatcctgg atgcaagcaa acagcggcat aacaaactat ggcgccagca
     1441 gcccacagat gacaccatcg cagagcccag tgagttcttg ccagagacct tggacggagc
     1501 cctggacgct cagctgccct gtttggatga taccacccac cctgggctcc cgagaagtct
     1561 ggccccagga ggacccgctc tcccctgttg ttttcgaaga ctctcggacc ccctcctcct
     1621 tccccaccat gatgaaacgg gtggcctggt ccacttagag gatctcgaga aggatgctct
     1681 gttagaggag gaggagtctc agccagtgga ggtgcacaag ctggttcagc atccccagga
     1741 aggtgccagg ctgtgtgaaa aggacgtaaa gaggaaactg gagtttggga actccaaacc
     1801 ccgcagtgac tccttgcctc aggtggagga gctggagaag gacggtagcc caagaacggg
     1861 gaggtggagg cgggcctcca cccagctcga tagaagcttg ctcgaccagg aaaacctaaa
     1921 taacaacaac agcaagagga gctgtcccga tgacttggag cgcgatgcca tgtttggaat
     1981 tctcagcaaa gtgaagcctc cctacacatc ctgtgccgac tgcatgtacc ccacagctgg
     2041 tgggactccc gaggcctaca tggagcgaca cgaagacccc agctcctctg ctatctgcac
     2101 ccagccaacc ttcctccccc atgtcacgtc ttccccaatg gcccacgcga gcagcaggtc
     2161 ccgagctccg gagaggccgg cctcgggtcc agccaacacc tctccattcc tactaccagc
     2221 aggctccagg aagccagatg tcagtggctc tggagccggg gctgccccgg aaccaccagc
     2281 aagccttcta gagccttcca gagagacctc aaaagccctt ccaaaatctc tccagttgaa
     2341 gaatcctcac tgtgacaaga acgctgccaa tatggaggtg tcggcgaagg aagaaccgtc
     2401 gcctaagaaa gatcccaagc cggctaagga cctgaggctt ctgttcagta acgaggcgga
     2461 gaagcccacc accaacagct acctgatgca gcatcaggag tccatcatcc aactgcagaa
     2521 ggcaggcctg gtccgaaagc acaccaaaga gctggagagg ttgaagagcc tgccttcaga
     2581 ctcaccagct gcctgcaggg acagcgccac ctgcaggctg gaggccagca tcccggagga
     2641 gggtagccag gagcccgcac acccagccct gtgcagccaa gctgggtcgg aagaacagcc
     2701 tgtaggggga accttgcaga agagccccac gtctaccctc ccccgtttag atcacaccag
     2761 taacttctcg aaggacttcc tgaagaccgt gtgttacacc cccacctcct cctccatcag
     2821 ctccaacctg acccggagct ccagcagcga cagcatccac agcgtccgag ggaagcctgg
     2881 gctggtgaag cagcgggcgc aggagatcga gacgcggctg cgcctggcag gcctcaccgt
     2941 gtcgtccccg ctgaaaaggt cccattccct tgccaagctg ggaagtctca acttctccac
     3001 ggaggacctg tccagcgagg ctgacacatc caccatcgct gactcgcagg acgccaagtg
     3061 tggtctctcc tcttccttct tgcccgaacc ccagtctgcg ccaagggacc ccgctgcaac
     3121 ctctaaatca tcagggaaat ctgccccaga acacttgaaa agcccgtcga gggtaaacaa
     3181 aagctgacta gctttcagct ctgtaaggtt ttcatcatgt cctgatctac tctcatgtct
     3241 tgatttgtgt taatgacttc agtcttctgg gctttcccca cttagatccc gtgagggggg
     3301 acacccgggt gtaggacctg ggcaacctgg aaactgtcag ccaagagtca cattcagata
     3361 tgtacacgca agccagggct acggcttggt tgttaagagt gtgtgcctga catgcaccag
     3421 gctctcagtt ttgatgccca gaaaccgtaa aaccaggcac ggttgcgcac gcgtacggaa
     3481 attccaacac tggaggtgca agcagaagga ttagaagtca tcttcagcta catggtgagt
     3541 tttaggccag cctggtggag agaaaaagaa aatgtgtgcg gacatacaaa atacagtttg
     3601 caacatgtca aggaaccaca ttggcgaggt cactgcagcc cacgatcccg gctcattccc
     3661 gtcctcgagg gagaatcctc agagggcaac acaaggccta cctctctgct cactgtgcat
     3721 ttttgtttat aaaacggtaa tgaccaaggc tcgttctgag aacaatgcta tgtccagaag
     3781 ggattttcca agagaattgt ggggggtttt gggtttggag ttttttttgt tgtttttttt
     3841 ttttgttttg ttttgttttt ttgagggtgg ggacccgcag aaatgtcaag actttgaacc
     3901 tgattgtccc aagaacagct aggtgatatc atcagaggaa agcacttagg gagagtggcc
     3961 ccgtctaatc ctccatcctg gtggggttgg gtcctcagct gttggcttcc cttcaagctt
     4021 atgtcaccca gtcactgaga aggcactgcg agcctcatct gctgctaacg gaggttgtgg
     4081 gcctggggtg tggatcatcc tgctgtgacc acacggtaac cctccaggat ggctcagtag
     4141 gaaccatctc cacacttgct gagaggaaag gcagcgtcag caacggatac tttcttacaa
     4201 gtccctcccc agagccagtc tgagaggaga gagtctattg aaaagctgct ctgatgttta
     4261 ccagagtccc ttcccgtgga gagaatagaa tattgcttcc ttgtctcctc cggctccccc
     4321 ttgctgtaat gatgggacaa aggaggcttg gccacagcac aggtgtgagc acagattagc
     4381 tcaatgaagc aggaggcctg ggctctgggt tcatatcatg ggcctcgact ggtgcccatc
     4441 atgaaaaggg tgcttaagga ttttttaaat tttttttttc cttaaggatt ttgaagagag
     4501 acaagcagag aggcagacca gcagaaagcc ttgacttggt atttagccaa cgatggtgca
     4561 cagccctgtg aggtcacata tccatttggg ctctgttaat gtgtttaggt tttggttttt
     4621 tgagacaggg tctcaccatg tacctccgct gtcttggaac tttctctgca gaccaggtag
     4681 gcctcggact cagcagcgat gcatctgcct ctgtctctca agggtttggt ttgggtattt
     4741 tggtttttga agtagactct caccctgtag ttcaagctgg cttcaaactc acagcagtcc
     4801 tcctgcctca gccttccaaa tgctgagatt acaggtataa tcccatcatg cctcagactt
     4861 ggttgtttac attttgaagg gtaaatggcg caggcttgtg atctacactc aggagggtga
     4921 ggcaggtcca ccatgagtta aaggccagtc tgggccacaa agtgagattc tacctcagaa
     4981 gtagtaacct tgccagagat ggtgttacct gcttcagatg tctgctgcga gccctctcag
     5041 aaagcttgct gatgccccaa ggctgcttgt aacactgcag gaagacgggg tttctatttg
     5101 aaggctgcct ttgctccttg gatatcacag accaactcct tccctcccac actccaaact
     5161 tgccagccta cccctttacc tcttgagcag atggagtcct tgtggagatg accacagctc
     5221 cttcctgcag ttggctggct cagccagaca gctcagtgag accacaaagt cttcaaggat
     5281 atgttccgga agtatgcgta tgggggtcct cccacactgg ttagaaccaa cggagtccgg
     5341 atggtttgga ggttgcagcc aggtcccttg gcacagcctg ggattcgggc aagaaggaag
     5401 aagagagacc gccatcctgg tgaacgctct gggtgtctcc aggatctggg ccagttctct
     5461 cttcattcgt ctggggtctt tgtgacctct gtctcagaac atttctccct ggcatgattt
     5521 ttttttttta aatacatgta tgttatttta tagggattta ttttcccact actgtcaata
     5581 ccagtcacct taaaaagcga tcatccactt attcctgaaa ccctgtgggt ttcttccact
     5641 cttccctcag ccaacaaaag caaaaaaaaa aaaaaaaaaa aaaaaaaaag gccaagttta
     5701 atgacatcgc caagttcaat ttcacatggt gggtcaattt ctgtgtcaaa ataattcctt
     5761 agatttggta atgataggct tccactgtgt ttcttaatcc catctgcaag agcgatagga
     5821 tttttgaaaa ctttcatttt gaaagagtca ggacaggagg atgaactcag gcaggcctct
     5881 cgctcccagc agactacctt acacctgttt gagaagcact ggccaatcag ccaacggtgc
     5941 gaacagcagg ggggcggatc cccatcttca gaccggaatc cctggcggga tcctgttcat
     6001 tccttggctc tctctgtaaa cacagtgaat tggcatttaa tggaaaggct actttggaac
     6061 tctccctgct aagctaagac ggtggcaact cccatattgt ctcaggaaaa gttttgttcc
     6121 agctaggctt ggtggcacac tctcgtgatg ccacgcagtg gatcactgag tccagagctt
     6181 tgtagacgct gaccaccacc cctcccccgc cccgccaaaa ataaaaaagt tagctttggg
     6241 agctgctcca cacctggcta ttttagcttt gtgtgtaatc atagcaagca gaggtagagg
     6301 gatttatgca gcgttgatga ctgccatttt cactgaatgc ccttgttgaa ctaagagctt
     6361 tctgctcagc tggatggccc gggaacttta catagccccc ccccccccca aagtctgaac
     6421 catgtggggg ccttgctcta caccaacaca gaaggcttcc tgaagtgtaa gggagaagat
     6481 actgggcaga ggtgggagcc tgatcgaatg aacaaagagc caggtgtctt tctcagatcc
     6541 tcacccccat gttttcagtg cagaagccct accaaccaca cacagctcat ccatgcggct
     6601 cagccatgct ggtctctccg aggtacccag atagctgcac ccctctgctc cccttgctca
     6661 gcagcttgct ctagctgaag atcaggccca gcatcctctg cccttgaaga gcccagaggg
     6721 ttctacagga tggaattaag ttctgaaaga ttaggtgttg acagccagcc tgtatttttg
     6781 tgcacccatc tcagggacca ccccggaaag cttgtgaatc tcccactctt caggagccat
     6841 ttctccttag caagaacaag atcacaagag acatcacaca tcaccccttt agggagttac
     6901 tttctacctg ctggcaaggt tggtatgaga gttgatttct aaccccaatg ggctggctga
     6961 tacttagcag ggctcttgag gcacgttcaa ccccttcagg gtaaccagag tacctaggac
     7021 cacttgcctg accctagctg tgcagtccag aagagccagg gaggctccca gtcctccccg
     7081 tgttggctgc aatgtcactg ctggtgaggg agactcttgg tcccaccttc ccatcctaac
     7141 ggctgggttc ctgctgatca taagaccaga tgaaggtcat tggtgttccc ctatccaaag
     7201 ccctttgttt ttcttactgt ggaggtgtga gagtccagtg tgtatggaga tatatgtaca
     7261 taagtgtatg aacatagacc ttgtgtgtgc attatagatc tatatacatg taagtgtatc
     7321 ctactccatc agacctggtg tgtgctgttc cttagcacac actgtggtct ccagctttgc
     7381 aaggaaatca ccctggaccc atctgcagga gccaccaggg gccatctgga gaagggcgat
     7441 cctttggcag tagacaccac agctcctctt tcagtcccta tcttagacac agcctctgtt
     7501 gccctggccg ttggccttgc tgcatgagaa gacagctgct tcctcctcta ccctgtaatt
     7561 attcttttta caattgagtg ccttaataat ggtttacaaa tactgtgtat ttatgcgcaa
     7621 ccgttcagct ctcgtctttc gaggctgggt acttttgtct ggtcctggtg gttaagatta
     7681 gcacgtgagt tcctccctac ccccatccac ttgtgttaat ctgctctgca gttgacactg
     7741 tgggtcctga ctactccaca gagcctgcta gaaactcggc tgcttggtcc ccgcaggcca
     7801 tgcctgcatg catcagcaca caccgcagcc cgaggtagcc ggtgagggac atgatttggt
     7861 ttgccaggta gcacaggggt ggtgcaggac cagtactccc tgaaccacag tccaagacct
     7921 ccccttagcc cctcaaggtc accctctagt ttatagaggc ttgccacccc ctaacaccct
     7981 tcacactccc tcctcctctt ctgcctgtgt cctgagctcc ttgctgcaaa tcatggggta
     8041 cagctcaccc cagaggagcc tcaccagact gcatccttca cctttcaaag gccactggca
     8101 ccgggttccc tcgttgagtc acaactttga ttttttttta attgtttttc tccttgtttt
     8161 cttttttaat atatatatat tatatattta aaaggcagta tctttttgta ctgtgaattt
     8221 tcagtagaag aggcatagtg cacttttttt tttacttctg ttggtgtgta ctgtatatat
     8281 atatagtctg cgcgcttctc gtgatgagaa taaacacttc gtttataaat gt
//
