LOCUS       NM_001083895            2871 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus sushi-repeat-containing protein, X-linked 2 (Srpx2),
            transcript variant 2, mRNA.
ACCESSION   NM_001083895
VERSION     NM_001083895.3
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Cong,Q., Soteros,B.M., Huo,A., Li,Y., Tenner,A.J. and Sia,G.M.
  TITLE     C1q and SRPX2 regulate microglia mediated synapse elimination
            during early development in the visual thalamus but not the visual
            cortex
  JOURNAL   Glia 70 (3), 451-465 (2022)
   PUBMED   34762332
  REMARK    GeneRIF: C1q and SRPX2 regulate microglia mediated synapse
            elimination during early development in the visual thalamus but not
            the visual cortex.
REFERENCE   3  (bases 1 to 2871)
  AUTHORS   Cong,Q., Soteros,B.M., Wollet,M., Kim,J.H. and Sia,G.M.
  TITLE     The endogenous neuronal complement inhibitor SRPX2 protects against
            complement-mediated synapse elimination during development
  JOURNAL   Nat Neurosci 23 (9), 1067-1078 (2020)
   PUBMED   32661396
  REMARK    GeneRIF: The endogenous neuronal complement inhibitor SRPX2
            protects against complement-mediated synapse elimination during
            development.
REFERENCE   4  (bases 1 to 2871)
  AUTHORS   Estrada,S.M., Thagard,A.S., Dehart,M.J., Damicis,J.R.,
            Dornisch,E.M., Ippolito,D.L., Burd,I., Napolitano,P.G. and
            Ieronimakis,N.
  TITLE     The orphan nuclear receptor Nr4a1 mediates perinatal
            neuroinflammation in a murine model of preterm labor
  JOURNAL   Cell Death Dis 11 (1), 11 (2020)
   PUBMED   31907354
  REMARK    Erratum:[Cell Death Dis. 2020 Jun 30;11(6):495. doi:
            10.1038/s41419-020-2682-y.. PMID: 32606386]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2871)
  AUTHORS   Anwer,M., Bolkvadze,T., Puhakka,N., Ndode-Ekane,X.E. and
            Pitkanen,A.
  TITLE     Genotype and Injury Effect on the Expression of a Novel
            Hypothalamic Protein Sushi Repeat-Containing Protein X-Linked 2
            (SRPX2)
  JOURNAL   Neuroscience 415, 184-200 (2019)
   PUBMED   31362033
  REMARK    GeneRIF: Unsupervised hierarchical clustering using SRPX2
            expression did not identify genotype or injury-specific clusters.
            Data demonstrate that SRPX2 expression in the hypothalamus is
            resistant to genetic deficiencies in the urokinase-system or to the
            hypothalamus-affecting TBI.
REFERENCE   6  (bases 1 to 2871)
  AUTHORS   Sia,G.M., Clem,R.L. and Huganir,R.L.
  TITLE     The human language-associated gene SRPX2 regulates synapse
            formation and vocalization in mice
  JOURNAL   Science 342 (6161), 987-991 (2013)
   PUBMED   24179158
REFERENCE   7  (bases 1 to 2871)
  AUTHORS   Abonnenc,M., Nabeebaccus,A.A., Mayr,U., Barallobre-Barreiro,J.,
            Dong,X., Cuello,F., Sur,S., Drozdov,I., Langley,S.R., Lu,R.,
            Stathopoulou,K., Didangelos,A., Yin,X., Zimmermann,W.H., Shah,A.M.,
            Zampetaki,A. and Mayr,M.
  TITLE     Extracellular matrix secretion by cardiac fibroblasts: role of
            microRNA-29b and microRNA-30c
  JOURNAL   Circ Res 113 (10), 1138-1147 (2013)
   PUBMED   24006456
REFERENCE   8  (bases 1 to 2871)
  AUTHORS   Naba,A., Clauser,K.R., Hoersch,S., Liu,H., Carr,S.A. and Hynes,R.O.
  TITLE     The matrisome: in silico definition and in vivo characterization by
            proteomics of normal and tumor extracellular matrices
  JOURNAL   Mol Cell Proteomics 11 (4), M111.014647 (2012)
   PUBMED   22159717
REFERENCE   9  (bases 1 to 2871)
  AUTHORS   Miljkovic-Licina,M., Hammel,P., Garrido-Urbani,S., Bradfield,P.F.,
            Szepetowski,P. and Imhof,B.A.
  TITLE     Sushi repeat protein X-linked 2, a novel mediator of angiogenesis
  JOURNAL   FASEB J 23 (12), 4105-4116 (2009)
   PUBMED   19667118
REFERENCE   10 (bases 1 to 2871)
  AUTHORS   Hackney,J.A., Charbord,P., Brunk,B.P., Stoeckert,C.J.,
            Lemischka,I.R. and Moore,K.A.
  TITLE     A molecular profile of a hematopoietic stem cell niche
  JOURNAL   Proc Natl Acad Sci U S A 99 (20), 13061-13066 (2002)
   PUBMED   12226475
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB843885.1, BB848735.1, AK136935.1 and AL691421.9.
            
            On Feb 12, 2013 this sequence version replaced NM_001083895.2.
            
            Transcript Variant: This variant (2) lacks an exon in the 5' UTR,
            compared to variant 1. Both variants 1 and 2 encode the same
            protein.The encoded protein contains an in-frame start site 41
            codons upstream of the currently annotated site, but Kozak sequence
            considerations and the presence of a signal peptide in the shorter
            version make the shorter version the more likely protein product.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK136935.1, SRR7652917.383555.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 BB843885.1         2-2
            2-146               BB848735.1         3-147
            147-1690            AK136935.1         2-1545
            1691-1691           AL691421.9         172028-172028
            1692-2358           AK136935.1         1547-2213
            2359-2359           AL691421.9         173683-173683
            2360-2871           AK136935.1         2215-2726
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 55.47 cM"
     gene            1..2871
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /note="sushi-repeat-containing protein, X-linked 2"
                     /db_xref="GeneID:68792"
                     /db_xref="MGI:MGI:1916042"
     exon            1..354
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /inference="alignment:Splign:2.1.0"
     exon            355..569
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /inference="alignment:Splign:2.1.0"
     CDS             485..1891
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /note="sushi-repeat containing protein; sushi
                     repeat-containing protein SRPX2"
                     /codon_start=1
                     /product="sushi repeat-containing protein SRPX2 precursor"
                     /protein_id="NP_001077364.2"
                     /db_xref="CCDS:CCDS41119.2"
                     /db_xref="GeneID:68792"
                     /db_xref="MGI:MGI:1916042"
                     /translation="MMTSPLTQRGALSLLLLLMPAVTPTWYAGSGYSPDESYNEVYAE
                     EVPAARARALDYRVPRWCYTLNIQDGEATCYSPRGGNYHSSLGTRCELSCDRGFRLIG
                     RKSVQCLPSRRWSGTAYCRQIRCHTLPFITSGTYTCTNGMLLDSRCDYSCSSGYHLEG
                     DRSRICMEDGRWSGGEPVCVDIDPPKIRCPHSREKMAEPEKLTARVYWDPPLVKDSAD
                     GTITRVTLRGPEPGSHFPEGEHVIRYTAYDRAYNRASCKFIVKVQVRRCPILKPPQHG
                     YLTCSSAGDNYGAICEYHCDGGYERQGTPSRVCQSSRQWSGTPPVCTPMKINVNVNSA
                     AGLLDQFYEKQRLLIVSAPDPSNRYYKMQISMLQQSTCGLDLRHVTIIELVGQPPQEV
                     GRIREQQLSAGIIEELRQFQRLTRSYFNMVLIDKQGIDRERYMEPVTPEEIFTFIDDY
                     LLSNEELARRVEQRDLCE"
     exon            570..656
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /inference="alignment:Splign:2.1.0"
     exon            657..848
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /inference="alignment:Splign:2.1.0"
     exon            849..1025
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1152
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1274
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1454
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1588
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1710
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..2871
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2215..2220
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /note="hexamer: AGTAAA"
     polyA_site      2239
                     /gene="Srpx2"
                     /gene_synonym="1110039C07Rik; SRP; SRPUL"
                     /note="major polyA site"
ORIGIN      
        1 actccaccca gagcttgaaa ccagtgacag tcacacttcc cctcttctgc agcagacagc
       61 actagctcct ctaatcctct tgcttccccc tcccccaacc atttcttggg gaataacaaa
      121 tatagctttg gggataatat agctttaaga cgacttttgg caaatgtaaa tgtcctaaca
      181 tctgggcagt gttaccagaa tcccggaggc cctgacagac caggagccac tggttctagg
      241 aatgttaaag tacaagggct ttttcccacc cccgactgac tgatgagagg agcagagagc
      301 aaagaaaaag aagagagatg aacgagacca caaaaatcat aaaataaaaa gcagatgtca
      361 tatgcctgct tcctctcact tttgggagtt catagctggt tctgctctga aagtcattcc
      421 cctttatcct ggtacttctg gccacatagc cctagtcttg tcttctgaag cttccctgtc
      481 gacgatgatg accagtccac tgactcagag aggtgctctc tcactgctgc tcctcctaat
      541 gcccgcagtg acaccaacat ggtacgcagg ctcaggttac tctccagatg aaagctacaa
      601 tgaagtatat gcagaagagg tccccgctgc ccgtgcccgt gccctggact acagagtccc
      661 ccgatggtgt tacacattga atatccagga tggagaagcc acatgctact caccaagggg
      721 aggaaattat cacagtagcc taggcacacg ttgtgagctc tcctgtgacc ggggctttcg
      781 attgattgga cggaagtcag tgcaatgttt gccaagccgg cgttggtctg gaactgccta
      841 ctgcaggcag ataaggtgcc acacactgcc attcatcact agtggcacct atacgtgcac
      901 caatggaatg ctgcttgact cccgttgtga ctatagctgt tctagtggct accacctaga
      961 aggagatcgc agccgcatct gcatggaaga tgggcgatgg agtggaggcg agcctgtatg
     1021 tgtagacata gatccaccca agatccgttg tcctcactct cgtgagaaaa tggcagagcc
     1081 agagaaacta actgctcgag tatactggga cccaccctta gtgaaagatt ctgctgatgg
     1141 taccatcacc agggtgacac ttcggggtcc agagcctggt tctcacttcc ctgaaggaga
     1201 acatgtgatt cgttatactg cctatgaccg agcctacaac cgggccagct gcaaattcat
     1261 tgtaaaagta caagtgagac gctgtcccat tctgaagcca ccacagcacg gctacctcac
     1321 ctgcagctca gcgggggaca actatggtgc catctgtgaa taccactgtg atggtggtta
     1381 tgaacgccag gggaccccat cccgagtctg ccagtcaagt cgacagtggt caggaacacc
     1441 acctgtctgt actcctatga agattaatgt caatgttaac tcggctgctg gcctcctgga
     1501 tcagttctat gagaaacagc gactcctcat agtctctgct cctgatccct ccaatcggta
     1561 ctacaaaatg caaatctcta tgctgcagca atccacctgt ggactcgacc tgcggcatgt
     1621 gaccattatt gagctggtgg gacagccacc tcaggaggtg gggcgcatcc gggagcaaca
     1681 actatcagca ggcatcattg aggagctcag gcaattccag cgcctcactc gttcctactt
     1741 caacatggtg ttaattgaca agcagggtat tgaccgggaa cgctatatgg aacctgtcac
     1801 ccctgaggaa atcttcacat tcatcgatga ctacctgctg agcaatgagg agctggcacg
     1861 gagagtggag cagagggacc tatgcgagtg accttgagtc agggtgtggc tgaagcacct
     1921 gtcctaggga gcttaaacta aggaggaaat gtcgtgtcct ccccccccca cacacacaca
     1981 cacacactgt tgggagcagc cctcgggtgg gggtgaggtt tgccaaatct aggacagttc
     2041 caggcagcat tttagggcag aatattcatt tccctaagca agtctccact tcgggtagca
     2101 ctggaggagc ctatgaaact gaggacatgc cctgggtggt gagttgtaga ccgaaggcct
     2161 tcttcacctg cctggagcct ctctctctag actcttccca aagcctttca gataagtaaa
     2221 taccaaattc attcctttac ggtgttgtaa atggttcctc taccctacaa taacagcagg
     2281 gggcagcatt gcaacagaca aacaagacac tttgaccaag tataaataga tttccctcac
     2341 agttagttat gtgaggaaca ggagagagga gactaggatc tacaaaacat tcttgaagct
     2401 gctcgtcatc ctctaggatg ctggccttaa aaacaatgtt gcttgagcca tttcttcatc
     2461 aagagttaga aaaacatttt ctccaggggg agtttgggaa gcaacgtcta gccatagctg
     2521 gctgttccct cctagatgct ccaactagct tgtaggcaag aacttctaat aactgaggtg
     2581 tttagtaccc tggtgacagc tcttccttag aggattctgc agtctgtgaa caacattacc
     2641 tctaagaatt agggagatgg ctccgttggt gaagtgcttg ctatcaagca ctgagacccg
     2701 agtgattccc agaacccatg taaaatagcc agatgtggtg gtgtgcactt ggaatcccag
     2761 cagagaggta gacataaaca gatctgtggg gatcactgct tagcaagcca agtctaatga
     2821 tgagtaccag gtcatgtaag aaacactatc tcaaaaacca tggtaaatgg c
//
