LOCUS       NP_001229340            1062 aa            linear   ROD 27-APR-2025
DEFINITION  SLIT-ROBO Rho GTPase-activating protein 1 isoform 2 [Mus musculus].
ACCESSION   NP_001229340
VERSION     NP_001229340.1
DBSOURCE    REFSEQ: accession NM_001242411.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 1062)
  AUTHORS   Rogg,M., Maier,J.I., Dotzauer,R., Artelt,N., Kretz,O.,
            Helmstadter,M., Abed,A., Sammarco,A., Sigle,A., Sellung,D.,
            Dinse,P., Reiche,K., Yasuda-Yamahara,M., Biniossek,M.L., Walz,G.,
            Werner,M., Endlich,N., Schilling,O., Huber,T.B. and Schell,C.
  TITLE     SRGAP1 Controls Small Rho GTPases To Regulate Podocyte Foot Process
            Maintenance
  JOURNAL   J Am Soc Nephrol 32 (3), 563-579 (2021)
   PUBMED   33514561
  REMARK    GeneRIF: SRGAP1 Controls Small Rho GTPases To Regulate Podocyte
            Foot Process Maintenance.
REFERENCE   2  (residues 1 to 1062)
  AUTHORS   Fan,X., Yang,H., Kumar,S., Tumelty,K.E., Pisarek-Horowitz,A.,
            Rasouly,H.M., Sharma,R., Chan,S., Tyminski,E., Shamashkin,M.,
            Belghasem,M., Henderson,J.M., Coyle,A.J., Salant,D.J., Berasi,S.P.
            and Lu,W.
  TITLE     SLIT2/ROBO2 signaling pathway inhibits nonmuscle myosin IIA
            activity and destabilizes kidney podocyte adhesion
  JOURNAL   JCI Insight 1 (19), e86934 (2016)
   PUBMED   27882344
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 1062)
  AUTHORS   Hwang,D.Y., Kohl,S., Fan,X., Vivante,A., Chan,S., Dworschak,G.C.,
            Schulz,J., van Eerde,A.M., Hilger,A.C., Gee,H.Y., Pennimpede,T.,
            Herrmann,B.G., van de Hoek,G., Renkema,K.Y., Schell,C., Huber,T.B.,
            Reutter,H.M., Soliman,N.A., Stajic,N., Bogdanovic,R., Kehinde,E.O.,
            Lifton,R.P., Tasic,V., Lu,W. and Hildebrandt,F.
  TITLE     Mutations of the SLIT2-ROBO2 pathway genes SLIT2 and SRGAP1 confer
            risk for congenital anomalies of the kidney and urinary tract
  JOURNAL   Hum Genet 134 (8), 905-916 (2015)
   PUBMED   26026792
  REMARK    GeneRIF: Mutations of the SLIT2-ROBO2 pathway genes SLIT2 and
            SRGAP1 confer risk for congenital anomalies of the kidney and
            urinary tract
REFERENCE   4  (residues 1 to 1062)
  AUTHORS   Chen,Z.B., Zhang,H.Y., Zhao,J.H., Zhao,W., Zhao,D., Zheng,L.F.,
            Zhang,X.F., Liao,X.P. and Yi,X.N.
  TITLE     Slit-Robo GTPase-activating proteins are differentially expressed
            in murine dorsal root ganglia: modulation by peripheral nerve
            injury
  JOURNAL   Anat Rec (Hoboken) 295 (4), 652-660 (2012)
   PUBMED   22271578
  REMARK    GeneRIF: srGAP1 and srGAP3 are largely expressed in subpopulations
            of dorsal root ganglion neurons following sciatic nerve lesion.
REFERENCE   5  (residues 1 to 1062)
  AUTHORS   Brunskill,E.W., Georgas,K., Rumballe,B., Little,M.H. and
            Potter,S.S.
  TITLE     Defining the molecular character of the developing and adult kidney
            podocyte
  JOURNAL   PLoS One 6 (9), e24640 (2011)
   PUBMED   21931791
REFERENCE   6  (residues 1 to 1062)
  AUTHORS   Bacon,C., Endris,V., Andermatt,I., Niederkofler,V., Waltereit,R.,
            Bartsch,D., Stoeckli,E.T. and Rappold,G.
  TITLE     Evidence for a role of srGAP3 in the positioning of commissural
            axons within the ventrolateral funiculus of the mouse spinal cord
  JOURNAL   PLoS One 6 (5), e19887 (2011)
   PUBMED   21655271
REFERENCE   7  (residues 1 to 1062)
  AUTHORS   Bacon,C., Endris,V. and Rappold,G.
  TITLE     Dynamic expression of the Slit-Robo GTPase activating protein genes
            during development of the murine nervous system
  JOURNAL   J Comp Neurol 513 (2), 224-236 (2009)
   PUBMED   19137586
  REMARK    GeneRIF: Data show that srGAP3 is expressed in ventricular zones of
            neurogenesis in many different tissues of the central nervous
            system.
REFERENCE   8  (residues 1 to 1062)
  AUTHORS   Wong,K., Ren,X.R., Huang,Y.Z., Xie,Y., Liu,G., Saito,H., Tang,H.,
            Wen,L., Brady-Kalnay,S.M., Mei,L., Wu,J.Y., Xiong,W.C. and Rao,Y.
  TITLE     Signal transduction in neuronal migration: roles of GTPase
            activating proteins and the small GTPase Cdc42 in the Slit-Robo
            pathway
  JOURNAL   Cell 107 (2), 209-221 (2001)
   PUBMED   11672528
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK138257.1, CB521559.1, CD351674.1, CX569081.1, BU706317.1,
            BU704234.1, CV556288.1 and AC153494.25.
            
            Transcript Variant: This variant (2) lacks an alternate coding
            exon, that causes a frameshift; however, it contains an alternate
            downstream exon that restores the reading frame. The resulting
            isoform (2) has a different internal segment compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC120893.1, SRR7345562.3877666.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 69.96 cM"
     Protein         1..1062
                     /product="SLIT-ROBO Rho GTPase-activating protein 1
                     isoform 2"
                     /note="Rho GTPase activating protein 13; rho
                     GTPase-activating protein 13"
                     /calculated_mol_wt=121299
     Region          26..278
                     /region_name="F-BAR_srGAP1"
                     /note="The F-BAR (FES-CIP4 Homology and
                     Bin/Amphiphysin/Rvs) domain of Slit-Robo GTPase Activating
                     Protein 1; cd07683"
                     /db_xref="CDD:153367"
     Site            order(31,34,41,44..45,48,51..52,55..56,59,63,66,70,110,
                     113,212,216,241,245,248,251..252,255,259,262,266,270,
                     273..274,277)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153367"
     Site            416
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q7Z6B7; propagated from
                     UniProtKB/Swiss-Prot (Q91Z69.2)"
     Region          482..666
                     /region_name="RhoGAP_srGAP"
                     /note="RhoGAP (GTPase-activator protein [GAP] for Rho-like
                     small GTPases) domain present in srGAPs. srGAPs are
                     components of the intracellular part of Slit-Robo
                     signalling pathway that is important for axon guidance and
                     cell migration. srGAPs contain an...; cd04383"
                     /db_xref="CDD:239848"
     Site            order(519,558,562,631,634..635,657)
                     /site_type="other"
                     /note="putative GTPase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239848"
     Site            519
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239848"
     Region          724..776
                     /region_name="SH3_srGAP1-3"
                     /note="Src homology 3 domain of Slit-Robo GTPase
                     Activating Proteins 1, 2, and 3; cd11955"
                     /db_xref="CDD:212888"
     Site            order(729,731,734,738,756..757,770,772..773)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212888"
     Region          785..931
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91Z69.2)"
     Site            812
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q7Z6B7; propagated from
                     UniProtKB/Swiss-Prot (Q91Z69.2)"
     Site            894
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q7Z6B7; propagated from
                     UniProtKB/Swiss-Prot (Q91Z69.2)"
     Site            909
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q7Z6B7; propagated from
                     UniProtKB/Swiss-Prot (Q91Z69.2)"
     Region          974..1013
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91Z69.2)"
     Site            976
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q7Z6B7; propagated from
                     UniProtKB/Swiss-Prot (Q91Z69.2)"
     Site            978
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q7Z6B7; propagated from
                     UniProtKB/Swiss-Prot (Q91Z69.2)"
     Site            1009
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q7Z6B7; propagated from
                     UniProtKB/Swiss-Prot (Q91Z69.2)"
     Region          1028..1062
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91Z69.2)"
     CDS             1..1062
                     /gene="Srgap1"
                     /gene_synonym="4930572H05Rik; Arhgap13"
                     /coded_by="NM_001242411.1:123..3311"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /db_xref="CCDS:CCDS56751.1"
                     /db_xref="GeneID:117600"
                     /db_xref="MGI:MGI:2152936"
ORIGIN      
        1 mstpsrfkkd keiiaeyesq vkeiraqlve qqkcleqqte mrvqllqdlq dffrkkaeie
       61 teysrnlekl aerfmaktrs tkdhqqfkkd qnllspvncw ylllnqvrre skdhatlsdi
      121 ylnnvimrfm qisedstrmf kkskeiafql hedlmkvlne lytvmktyhm yhsesisaes
      181 klkeaekqee kqigrsgdpv fhirleerhq rrssvkkiek mkekrqakys enklksikar
      241 neylltleat nasvfkyyih dlsdlidccd lgyhaslnra lrtylsaeyn letsrhegld
      301 iienavdnle prsdkqrfme mypaafcppm kfefqshmgd evcqvsaqqp vqaelmlrnq
      361 qlqsrlatlk ieseevkktt eatlqtiqdm vtiedydvse cfqhsrstes vkstvsetyl
      421 skpsiakrra nqqeteqfyf mklreflegs nlitklqakh dllqrtlgeg hraeymttsr
      481 grrnsharhq dsgqvipliv escirfinly glqhqgifrv sgsqvevndi knsfergenp
      541 lsdeqsnhdi nsvagvlkly frglenplfp kerftdlisc iridnlyera lhirkllltl
      601 prsvlivmry lfaflnhlsq ysdenmmdpy nlaicfgptl mpvpeiqdqv scqahvneiv
      661 ktiiihheti fpdakeldgp vyekcmaggd ycdspysehg tleevdqdag tephtsedec
      721 epieaiakfd yvgrsarels fkkgasllly hrasedwweg rhngidglvp hqyivvqdmd
      781 dtfsdtlsqk adseassgpv tedkssskdm nsptdrhsds ylarqrkrge ppppgrrpgr
      841 tsdghcplhp phalsnssid lgspnlashp rgllqnrgln ndsperrrrp ghgsltnisr
      901 hdslkkidsp pirrstssgq ytgfndhkpl dpetiaqdie etmntalnel relerqstvk
      961 hapdvvldtl eqvknsptpa tsteslsplh nvalrgsepq irrstsssse tmstfkpmva
     1021 prmgvqlkpp alrpkpavlp ktnptmgpaa psqgptdksc tm
//
