LOCUS       NM_020611               1769 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus steroid 5 alpha-reductase 3 (Srd5a3), mRNA.
ACCESSION   NM_020611
VERSION     NM_020611.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Sha,Q.Q., Zhu,Y.Z., Li,S., Jiang,Y., Chen,L., Sun,X.H., Shen,L.,
            Ou,X.H. and Fan,H.Y.
  TITLE     Characterization of zygotic genome activation-dependent maternal
            mRNA clearance in mouse
  JOURNAL   Nucleic Acids Res 48 (2), 879-894 (2020)
   PUBMED   31777931
REFERENCE   3  (bases 1 to 1769)
  AUTHORS   Medina-Cano,D., Ucuncu,E., Nguyen,L.S., Nicouleau,M., Lipecka,J.,
            Bizot,J.C., Thiel,C., Foulquier,F., Lefort,N., Faivre-Sarrailh,C.,
            Colleaux,L., Guerrera,I.C. and Cantagrel,V.
  TITLE     High N-glycan multiplicity is critical for neuronal adhesion and
            sensitizes the developing cerebellum to N-glycosylation defect
  JOURNAL   Elife 7, e38309 (2018)
   PUBMED   30311906
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1769)
  AUTHORS   Sundberg,J.P., Dadras,S.S., Silva,K.A., Kennedy,V.E., Garland,G.,
            Murray,S.A., Sundberg,B.A., Schofield,P.N. and Pratt,C.H.
  TITLE     Systematic screening for skin, hair, and nail abnormalities in a
            large-scale knockout mouse program
  JOURNAL   PLoS One 12 (7), e0180682 (2017)
   PUBMED   28700664
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1769)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 1769)
  AUTHORS   Mizuno,S., Takami,K., Daitoku,Y., Tanimoto,Y., Dinh,T.T.,
            Mizuno-Iijima,S., Hasegawa,Y., Takahashi,S., Sugiyama,F. and
            Yagami,K.
  TITLE     Peri-implantation lethality in mice carrying megabase-scale
            deletion on 5qc3.3 is caused by Exoc1 null mutation
  JOURNAL   Sci Rep 5, 13632 (2015)
   PUBMED   26346620
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1769)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   8  (bases 1 to 1769)
  AUTHORS   Cantagrel,V., Lefeber,D.J., Ng,B.G., Guan,Z., Silhavy,J.L.,
            Bielas,S.L., Lehle,L., Hombauer,H., Adamowicz,M., Swiezewska,E., De
            Brouwer,A.P., Blumel,P., Sykut-Cegielska,J., Houliston,S.,
            Swistun,D., Ali,B.R., Dobyns,W.B., Babovic-Vuksanovic,D., van
            Bokhoven,H., Wevers,R.A., Raetz,C.R., Freeze,H.H., Morava,E.,
            Al-Gazali,L. and Gleeson,J.G.
  TITLE     SRD5A3 is required for converting polyprenol to dolichol and is
            mutated in a congenital glycosylation disorder
  JOURNAL   Cell 142 (2), 203-217 (2010)
   PUBMED   20637498
  REMARK    GeneRIF: Srd5a3 mutation is lethal in mouse and results in an
            activation of the unfolded protein response pathway.
REFERENCE   9  (bases 1 to 1769)
  AUTHORS   Wilsbacher,L.D., Sangoram,A.M., Antoch,M.P. and Takahashi,J.S.
  TITLE     The mouse Clock locus: sequence and comparative analysis of 204 kb
            from mouse chromosome 5
  JOURNAL   Genome Res 10 (12), 1928-1940 (2000)
   PUBMED   11116088
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AI553072.1, AK075635.1 and AK164282.1.
            
            On Mar 23, 2011 this sequence version replaced NM_020611.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK075635.1, AK162527.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-402               AI553072.1         3-404
            403-1737            AK075635.1         399-1733
            1738-1769           AK164282.1         1840-1871
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 40.5 cM"
     gene            1..1769
                     /gene="Srd5a3"
                     /gene_synonym="1110025P14Rik; A430076C09; D730040M03Rik;
                     H5ar; S5AR 3; Srd5a2l"
                     /note="steroid 5 alpha-reductase 3"
                     /db_xref="GeneID:57357"
                     /db_xref="MGI:MGI:1930252"
     exon            1..276
                     /gene="Srd5a3"
                     /gene_synonym="1110025P14Rik; A430076C09; D730040M03Rik;
                     H5ar; S5AR 3; Srd5a2l"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..1048
                     /gene="Srd5a3"
                     /gene_synonym="1110025P14Rik; A430076C09; D730040M03Rik;
                     H5ar; S5AR 3; Srd5a2l"
                     /EC_number="1.3.1.22"
                     /EC_number="1.3.1.94"
                     /note="steroid 5 alpha-reductase 2 like; H5AR;
                     3-oxo-5-alpha-steroid 4-dehydrogenase 3; probable
                     polyprenol reductase; SR type 3; steroid 5 alpha-reductase
                     2-like; H5AR gene; steroid 5 alpha-reductase 2 like"
                     /codon_start=1
                     /product="polyprenal reductase"
                     /protein_id="NP_065636.2"
                     /db_xref="CCDS:CCDS19358.1"
                     /db_xref="GeneID:57357"
                     /db_xref="MGI:MGI:1930252"
                     /translation="MAGWAGFELSALNPLRTLWLALAAAFLFALLLQLAPARLLPSCA
                     LFQDLLRYGKTKQSGSRRPAVCRAFDVPKRYFSHFYVISVVWNGSLLWLLSQSLFLGA
                     PFPNWLSALLRTLGATQFQALEMESKASRMPAAELALSAFLVLVFLWVHSLRRLFECF
                     YVSVFSNAAIHVVQYCFGLVYYVLVGLTVLSQVPMDDKNVYVLGKNLLIQARWFHILG
                     MVMFFWSSAHQYKCHVILSNLRRNKKGVVIHCQHRIPFGDWFEYVSSANYLAELMIYI
                     SMAVTFGLHNLTWWLVVTYVFSSQALSAFFNHKFYRSTFVSYPKHRKAFLPFLF"
     exon            277..419
                     /gene="Srd5a3"
                     /gene_synonym="1110025P14Rik; A430076C09; D730040M03Rik;
                     H5ar; S5AR 3; Srd5a2l"
                     /inference="alignment:Splign:2.1.0"
     exon            420..455
                     /gene="Srd5a3"
                     /gene_synonym="1110025P14Rik; A430076C09; D730040M03Rik;
                     H5ar; S5AR 3; Srd5a2l"
                     /inference="alignment:Splign:2.1.0"
     exon            456..653
                     /gene="Srd5a3"
                     /gene_synonym="1110025P14Rik; A430076C09; D730040M03Rik;
                     H5ar; S5AR 3; Srd5a2l"
                     /inference="alignment:Splign:2.1.0"
     exon            654..788
                     /gene="Srd5a3"
                     /gene_synonym="1110025P14Rik; A430076C09; D730040M03Rik;
                     H5ar; S5AR 3; Srd5a2l"
                     /inference="alignment:Splign:2.1.0"
     exon            789..1769
                     /gene="Srd5a3"
                     /gene_synonym="1110025P14Rik; A430076C09; D730040M03Rik;
                     H5ar; S5AR 3; Srd5a2l"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1474..1479
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Srd5a3"
                     /gene_synonym="1110025P14Rik; A430076C09; D730040M03Rik;
                     H5ar; S5AR 3; Srd5a2l"
                     /note="hexamer: AATAAA"
     regulatory      1491..1496
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Srd5a3"
                     /gene_synonym="1110025P14Rik; A430076C09; D730040M03Rik;
                     H5ar; S5AR 3; Srd5a2l"
                     /note="hexamer: AATAAA"
     polyA_site      1498
                     /gene="Srd5a3"
                     /gene_synonym="1110025P14Rik; A430076C09; D730040M03Rik;
                     H5ar; S5AR 3; Srd5a2l"
                     /note="major polyA site"
     polyA_site      1517
                     /gene="Srd5a3"
                     /gene_synonym="1110025P14Rik; A430076C09; D730040M03Rik;
                     H5ar; S5AR 3; Srd5a2l"
     regulatory      1714..1719
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Srd5a3"
                     /gene_synonym="1110025P14Rik; A430076C09; D730040M03Rik;
                     H5ar; S5AR 3; Srd5a2l"
                     /note="hexamer: AATAAA"
     polyA_site      1738
                     /gene="Srd5a3"
                     /gene_synonym="1110025P14Rik; A430076C09; D730040M03Rik;
                     H5ar; S5AR 3; Srd5a2l"
                     /note="major polyA site"
ORIGIN      
        1 gggtgagaga aggagggcgg cgcggcggcg cgcatctgta gccggctccc gggctatggc
       61 tgggtgggcc gggtttgagc tctcggccct gaaccccctg cggacgctgt ggctggcgct
      121 ggccgccgcc ttcctgttcg cgctgctgct gcagctggcg cccgccaggc tgctgccgag
      181 ctgcgcgctc ttccaggacc tgctccgcta cgggaagacc aagcagtccg gctcgcggcg
      241 ccccgccgtc tgcagggcct tcgatgtccc caagaggtac ttttctcact tctacgtcat
      301 ctcagttgtg tggaatggct ccctgctctg gctactttct cagtcgttgt tcctgggagc
      361 accttttcca aactggctta gtgctctgct cagaactctt ggggccacac agttccaagc
      421 cctggagatg gagtccaagg cttctcggat gccagcggct gagctggctc tgtctgcctt
      481 cttggtcttg gtgttcctct gggtccacag ccttcggaga ctctttgagt gcttctacgt
      541 cagtgtcttc tctaatgcag ccattcacgt tgtgcagtac tgtttcgggc ttgtctacta
      601 tgtgcttgtt ggcctgactg tactgagcca agtgcccatg gatgataaga atgtgtatgt
      661 tctggggaag aatctactga tacaagcccg gtggttccac atcctgggca tggtgatgtt
      721 cttctggtca tccgcccatc agtataaatg ccacgtcatc ctcagcaacc tcaggagaaa
      781 caaaaaaggt gtggtcatcc actgccagca ccggatcccc tttggagact ggttcgagta
      841 cgtgtcttca gctaactacc tagcagagct gatgatctac atctccatgg ctgtcacctt
      901 tgggctccac aacttaacct ggtggctggt ggtgacgtat gtcttctcca gccaagcctt
      961 gtccgcattc ttcaaccaca agttctacag aagcacattt gtctcctacc caaagcatag
     1021 gaaagcgttc ctcccatttt tgttttaaag cgggctttat ggtgaagaaa gccaggtgac
     1081 agattccatt cctagaggca ctgagacaga gaccaaagta cactttctgc gggaatgttt
     1141 gacggtcctt gttctacttc agagccagcc gagcagtgtt caccgagcga ggcgttattc
     1201 ctggagaaca cattccagca gacctaggct tgcaggatcg gctttctgcc aagctttaca
     1261 gagctaactg ataacaagta aacgggtgtc tccaaactgc tttctggccg cactaaccag
     1321 tataaacagc tgtgcatgga tacatgcatg cttgagtcag tctctctctc tctctctctc
     1381 tctctatctc tctctctctc tctctctctc tctctctctc tagctgtggg aacagttaac
     1441 tgcactgagt gctgtgggag atcataggtt tttaataaat gtcacatgcc aataaaaaca
     1501 ggaaactctg aaaataatat gaatgtacag tatcagaccg gtggttccag ggagtaaaga
     1561 gtttgttgga gtgagttaac attcttcctt ttctcaccga tatctcccgt tatagcccca
     1621 ctccctcact tgccctgcaa ccaacctaat aataagttta gaaacttctt tgatggaatt
     1681 ctctttttga tgtttgtact gaaaacatcg agaaataaaa tatttttata cgtatgcata
     1741 cagcacgccc tggactgggt aacatggaa
//
