LOCUS       NM_009270               2748 bp    mRNA    linear   ROD 21-MAY-2024
DEFINITION  Mus musculus squalene epoxidase (Sqle), mRNA.
ACCESSION   NM_009270
VERSION     NM_009270.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Liu,Y., Wang,Z., Jin,H., Cui,L., Huo,B., Xie,C., Li,J., Ding,H.,
            Zhang,H., Xiong,W., Li,M., Zhang,H., Guo,H., Li,C., Wang,T.,
            Wang,X., He,W., Wang,Z., Bei,J.X., Huang,P., Liu,J. and Xia,X.
  TITLE     Squalene-epoxidase-catalyzed 24(S),25-epoxycholesterol synthesis
            promotes trained-immunity-mediated antitumor activity
  JOURNAL   Cell Rep 43 (4), 114094 (2024)
   PUBMED   38613784
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2748)
  AUTHORS   Ma,L., Huang,W., Liang,X., Bai,G., Wang,X., Jiang,H., Xin,Y.,
            Hu,L., Chen,X. and Liu,C.
  TITLE     Inhibition of squalene epoxidase linking with PI3K/AKT signaling
            pathway suppresses endometrial cancer
  JOURNAL   Cancer Sci 114 (9), 3595-3607 (2023)
   PUBMED   37438885
  REMARK    GeneRIF: Inhibition of squalene epoxidase linking with PI3K/AKT
            signaling pathway suppresses endometrial cancer.
REFERENCE   4  (bases 1 to 2748)
  AUTHORS   Li,C., Wang,Y., Liu,D., Wong,C.C., Coker,O.O., Zhang,X., Liu,C.,
            Zhou,Y., Liu,Y., Kang,W., To,K.F., Sung,J.J. and Yu,J.
  TITLE     Squalene epoxidase drives cancer cell proliferation and promotes
            gut dysbiosis to accelerate colorectal carcinogenesis
  JOURNAL   Gut 71 (11), 2253-2265 (2022)
   PUBMED   35232776
  REMARK    GeneRIF: Squalene epoxidase drives cancer cell proliferation and
            promotes gut dysbiosis to accelerate colorectal carcinogenesis.
REFERENCE   5  (bases 1 to 2748)
  AUTHORS   Nguyen,K.T., Mun,S.H., Yang,J., Lee,J., Seok,O.H., Kim,E., Kim,D.,
            An,S.Y., Seo,D.Y., Suh,J.Y., Lee,Y. and Hwang,C.S.
  TITLE     The MARCHF6 E3 ubiquitin ligase acts as an NADPH sensor for the
            regulation of ferroptosis
  JOURNAL   Nat Cell Biol 24 (8), 1239-1251 (2022)
   PUBMED   35941365
REFERENCE   6  (bases 1 to 2748)
  AUTHORS   Cai,J., Ash,D., Kotch,L.E., Jabs,E.W., Attie-Bitach,T., Auge,J.,
            Mattei,G., Etchevers,H., Vekemans,M., Korshunova,Y., Tidwell,R.,
            Messina,D.N., Winston,J.B. and Lovett,M.
  TITLE     Gene expression in pharyngeal arch 1 during human embryonic
            development
  JOURNAL   Hum Mol Genet 14 (7), 903-912 (2005)
   PUBMED   15703188
REFERENCE   7  (bases 1 to 2748)
  AUTHORS   Stylianou,I.M., Clinton,M., Keightley,P.D., Pritchard,C.,
            Tymowska-Lalanne,Z., Bunger,L. and Horvat,S.
  TITLE     Microarray gene expression analysis of the Fob3b obesity QTL
            identifies positional candidate gene Sqle and perturbed cholesterol
            and glycolysis pathways
  JOURNAL   Physiol Genomics 20 (3), 224-232 (2005)
   PUBMED   15598878
  REMARK    GeneRIF: Squalene epoxidase is a strong positional candidate gene
            for obesity, using squantitative trait locus studies.
REFERENCE   8  (bases 1 to 2748)
  AUTHORS   Stylianou,I.M., Christians,J.K., Keightley,P.D., Bunger,L.,
            Clinton,M., Bulfield,G. and Horvat,S.
  TITLE     Genetic complexity of an obesity QTL (Fob3) revealed by detailed
            genetic mapping
  JOURNAL   Mamm Genome 15 (6), 472-481 (2004)
   PUBMED   15181539
REFERENCE   9  (bases 1 to 2748)
  AUTHORS   Ono,T.
  TITLE     The first step of oxygenation in cholesterol biosynthesis
  JOURNAL   Biochem Biophys Res Commun 292 (5), 1283-1288 (2002)
   PUBMED   11969229
REFERENCE   10 (bases 1 to 2748)
  AUTHORS   Kosuga,K., Hata,S., Osumi,T., Sakakibara,J. and Ono,T.
  TITLE     Nucleotide sequence of a cDNA for mouse squalene epoxidase
  JOURNAL   Biochim Biophys Acta 1260 (3), 345-348 (1995)
   PUBMED   7873613
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC113269.13.
            
            On Nov 17, 2006 this sequence version replaced NM_009270.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC056361.1, AK163502.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1038              AC113269.13        122301-123338
            1039-1291           AC113269.13        125005-125257
            1292-1472           AC113269.13        128523-128703
            1473-1569           AC113269.13        130045-130141
            1570-1683           AC113269.13        130823-130936
            1684-1855           AC113269.13        131016-131187
            1856-1951           AC113269.13        131671-131766
            1952-2094           AC113269.13        133244-133386
            2095-2191           AC113269.13        133779-133875
            2192-2279           AC113269.13        136949-137036
            2280-2748           AC113269.13        137934-138402
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 25.16 cM"
     gene            1..2748
                     /gene="Sqle"
                     /note="squalene epoxidase"
                     /db_xref="GeneID:20775"
                     /db_xref="MGI:MGI:109296"
     exon            1..1038
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     CDS             754..2472
                     /gene="Sqle"
                     /EC_number="1.14.14.17"
                     /note="SE"
                     /codon_start=1
                     /product="squalene monooxygenase"
                     /protein_id="NP_033296.1"
                     /db_xref="CCDS:CCDS27497.1"
                     /db_xref="GeneID:20775"
                     /db_xref="MGI:MGI:109296"
                     /translation="MWTFLGIATFTYFYKKCGDVTLANKELLLCVLVFLSLGLVLSYR
                     CRHRHGGLLGRHQSGAQFAAFSDILSALPLIGFFWAKSPESEKKEQLESKKCRKEIGL
                     SETTLTGAATSVSTSFVTDPEVIIVGSGVLGSALAAVLSRDGRKVTVIERDLKEPDRI
                     VGELLQPGGYRVLQELGLGDTVEGLNAHHIHGYIVHDYESRSEVQIPYPLSETNQVQS
                     GIAFHHGRFIMSLRKAAMAEPNVKFIEGVVLQLLEEDDAVIGVQYKDKETGDTKELHA
                     PLTVVADGLFSKFRKSLISSKVSVSSHFVGFLMKDAPQFKPNFAELVLVNPSPVLIYQ
                     ISSSETRVLVDIRGELPRNLREYMAEQIYPQLPEHLKESFLEASQNGRLRTMPASFLP
                     PSSVNKRGVLILGDAYNLRHPLTGGGMTVALKDIKLWRQLLKDIPDLYDDAAIFQAKK
                     SFFWSRKRTHSFVVNVLAQALYELFSATDDSLHQLRKACFLYFKLGGECVTGPVGLLS
                     ILSPHPLVLIRHFFSVAIYATYFCFKSEPWATKPRALFSSGAVLYKACSILFPLIYSE
                     MKYLVH"
     exon            1039..1291
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1472
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1569
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1683
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1855
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            1856..1951
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            1952..2094
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            2095..2191
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            2192..2279
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            2280..2748
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2725..2730
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sqle"
                     /note="hexamer: AATAAA"
     polyA_site      2748
                     /gene="Sqle"
                     /note="major polyA site"
ORIGIN      
        1 gcgagcgggg cgcgcagcgg ttactctggt tactgtccgc gagggatgct ggggacccgg
       61 cgggcaggag ccttcgcctc gctctgaagg acgctcatcc gagacagcca ttcactctcg
      121 aggaaggcaa gtgtcggtcg gacaggttcc cggccgtgac ttcggggctt gcttgaggag
      181 gaatctccaa gggagggctg agctgccagg ctgccgccgc gcttcgggac gccgtggcca
      241 tcttttgttg gagaaggctg ccggggcagt gttgctaagg gcagctttcc cagcacccag
      301 atccttctac gctcccgact ccttcctacc gagcacctgc tgctggtgac aaaagaccgt
      361 ttacagccag gcgagcggac atcgaagtat acagccacat tcgcacccct cagcccaata
      421 gagaggcccg tgtccatcag ttggctgaat gaagaagtgt gtaatcagga gccgttgggg
      481 taggcaggct ttaaatgctg ccttttaaga tctggacaac ttaagataca tttgcactct
      541 caatgatttc aaaaattgat cacatctgac tcgtgatcct gaaactattt attggaagaa
      601 gttcgaccgg ttgccccgaa cccctttgag aactacattt tggggacatt ttaatcactg
      661 aagaccttcc gaggaaaaaa gaccccccca agagaccccc aaaacacaaa atcctcagcc
      721 ttggctggtg ccaccaaaga agccttttga gccatgtgga cttttcttgg cattgctacc
      781 ttcacctatt tttataagaa atgcggggat gtcaccctgg ccaacaagga gctcctgctg
      841 tgcgtgctgg tgttcctgtc gctgggcctg gtgctctcct accgctgtcg ccatcgacac
      901 gggggcctcc ttggccgcca tcagagcggc gcccagttcg ctgccttctc ggatattctc
      961 tctgctttgc ctcttattgg cttcttctgg gccaagtcac ctgaatcaga aaagaaagaa
     1021 cagctggagt ccaagaagtg cagaaaagaa atcggtcttt cagaaacgac attaacagga
     1081 gcagctacct cagtgtcgac ctcgttcgtg acggacccgg aagtgatcat cgtgggatct
     1141 ggtgtgcttg gatctgcctt ggcagcagtt ctctccagag atggaagaaa ggtgacggtc
     1201 atcgagagag atttaaaaga gcccgacagg atagttgggg agctgctgca gccaggaggc
     1261 taccgtgttc tccaggagct gggccttgga gatacagtag aaggtctcaa tgcccatcat
     1321 atacatggct acatagttca tgactatgaa agcagatctg aagttcaaat tccgtaccca
     1381 ctgtcagaaa ccaaccaagt gcagagtgga attgcttttc accatggccg attcatcatg
     1441 agtctccgga aagcagctat ggcagagccc aatgtaaagt ttatagaagg tgttgtgctt
     1501 cagttactag aggaagatga tgctgtaatc ggcgtgcaat acaaggacaa ggagactggg
     1561 gacaccaagg agctccacgc cccgctcacc gttgttgcgg atggactctt ctccaagttc
     1621 aggaagagcc tcatctccag taaagtctcc gtttcttccc acttcgttgg cttccttatg
     1681 aaggatgcac cacagtttaa acccaatttt gcggagcttg ttctggtcaa ccccagtcca
     1741 gttctcatct accagatttc ttccagtgaa actagggtac ttgttgatat tcggggagaa
     1801 ttgccaagaa acctaagaga atacatggct gaacaaattt acccacagtt acctgagcac
     1861 ctgaaggagt catttctgga ggcctctcag aatggtcgtc tgcggaccat gccggccagc
     1921 ttccttcctc cttcctcagt gaacaaacga ggcgtcctta ttctgggaga tgcgtataac
     1981 ctgaggcacc ctcttactgg tggaggaatg acagttgctt taaaagatat aaaattgtgg
     2041 agacaactgt taaaagacat tcctgacctt tatgatgatg ctgctatttt ccaggccaaa
     2101 aaatcattct tttggtcaag aaaaaggacc cattcctttg ttgtgaatgt attggctcag
     2161 gccttgtatg aattattttc agctacagat gattccttgc atcagctccg aaaagcttgc
     2221 tttctgtatt ttaaacttgg tggagagtgt gtgaccggtc ctgttgggtt gctttcaata
     2281 ttgtctcctc accctctggt gttgattcga cacttctttt ccgttgcaat ctacgccacg
     2341 tatttctgct tcaaatcaga gccgtgggct acaaaaccac gggccctttt cagtagcggt
     2401 gctgtactgt acaaagcgtg ttctatccta tttccactca tctattcaga aatgaagtat
     2461 ctggttcatt gaaagggagt ccacgtgcag tcaagtccct tggggcttga agaggatgta
     2521 tatagcatag aactgtgtca cttccacagt ggacttgagg accaagcttg tgtataagca
     2581 tgtaaatata agccttactt tacagttgaa aatgaaggtc agagaagcta gatgtttaaa
     2641 aggatgattg tttctacaaa tcagtgctaa cactcaagtt tttcttttga tttcagtatt
     2701 tggggtgact cattgggaca agccaataaa atgaagagtg aactctta
//
