LOCUS       NM_001242345            1536 bp    mRNA    linear   ROD 17-SEP-2024
DEFINITION  Mus musculus spexin hormone (Spx), transcript variant 1, mRNA.
ACCESSION   NM_001242345 XM_620381 XM_908052
VERSION     NM_001242345.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Butts,J.C., Wu,S.R., Durham,M.A., Dhindsa,R.S., Revelli,J.P.,
            Ljungberg,M.C., Saulnier,O., McLaren,M.E., Taylor,M.D. and
            Zoghbi,H.Y.
  TITLE     A single-cell transcriptomic map of the developing Atoh1 lineage
            identifies neural fate decisions and neuronal diversity in the
            hindbrain
  JOURNAL   Dev Cell 59 (16), 2171-2188 (2024)
   PUBMED   39106860
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Li,D., Liu,Y., Li,C., Zhou,Z., Gao,K., Bao,H., Yang,J., Xue,G.,
            Yin,D., Zhao,X., Shen,K., Zhang,L., Li,J., Li,C., Song,J., Zhao,L.,
            Pei,Y., Xuan,L., Zhang,Y., Lu,Y., Zhang,Z.R., Yang,B., Li,Y. and
            Pan,Z.
  TITLE     Spexin Diminishes Atrial Fibrillation Vulnerability by Acting on
            Galanin Receptor 2
  JOURNAL   Circulation 150 (2), 111-127 (2024)
   PUBMED   38726666
  REMARK    GeneRIF: Spexin Diminishes Atrial Fibrillation Vulnerability by
            Acting on Galanin Receptor 2.
REFERENCE   3  (bases 1 to 1536)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1536)
  AUTHORS   Chen,Y., He,M., Lei,M.M.L., Ko,W.K.W., Lin,C., Bian,Z. and
            Wong,A.O.L.
  TITLE     Mouse Spexin: (III) Differential Regulation by Glucose and Insulin
            in Glandular Stomach and Functional Implication in Feeding Control
  JOURNAL   Front Endocrinol (Lausanne) 12, 681648 (2021)
   PUBMED   34025589
  REMARK    GeneRIF: Mouse Spexin: (III) Differential Regulation by Glucose and
            Insulin in Glandular Stomach and Functional Implication in Feeding
            Control.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1536)
  AUTHORS   Zhuang,M., Lai,Q., Yang,C., Ma,Y., Fan,B., Bian,Z., Lin,C., Bai,J.
            and Zeng,G.
  TITLE     Spexin as an anxiety regulator in mouse hippocampus: Mechanisms for
            transcriptional regulation of spexin gene expression by
            corticotropin releasing factor
  JOURNAL   Biochem Biophys Res Commun 525 (2), 326-333 (2020)
   PUBMED   32093887
  REMARK    GeneRIF: Spexin as an anxiety regulator in mouse hippocampus:
            Mechanisms for transcriptional regulation of spexin gene expression
            by corticotropin releasing factor.
REFERENCE   6  (bases 1 to 1536)
  AUTHORS   Walewski,J.L., Ge,F., Lobdell,H. 4th, Levin,N., Schwartz,G.J.,
            Vasselli,J.R., Pomp,A., Dakin,G. and Berk,P.D.
  TITLE     Spexin is a novel human peptide that reduces adipocyte uptake of
            long chain fatty acids and causes weight loss in rodents with
            diet-induced obesity
  JOURNAL   Obesity (Silver Spring) 22 (7), 1643-1652 (2014)
   PUBMED   24550067
  REMARK    GeneRIF: Spexin is a novel hormone involved in weight regulation,
            with potential for obesity therapy.
REFERENCE   7  (bases 1 to 1536)
  AUTHORS   Toll,L., Khroyan,T.V., Sonmez,K., Ozawa,A., Lindberg,I.,
            McLaughlin,J.P., Eans,S.O., Shahien,A.A. and Kapusta,D.R.
  TITLE     Peptides derived from the prohormone proNPQ/spexin are potent
            central modulators of cardiovascular and renal function and
            nociception
  JOURNAL   FASEB J 26 (2), 947-954 (2012)
   PUBMED   22038051
  REMARK    GeneRIF: newly identified peptides derived from the NPQ/spexin
            precursor contribute to CNS-mediated control of arterial blood
            pressure and salt and water balance and modulate nociceptive
            responses
REFERENCE   8  (bases 1 to 1536)
  AUTHORS   Quina,L.A., Wang,S., Ng,L. and Turner,E.E.
  TITLE     Brn3a and Nurr1 mediate a gene regulatory pathway for habenula
            development
  JOURNAL   J Neurosci 29 (45), 14309-14322 (2009)
   PUBMED   19906978
REFERENCE   9  (bases 1 to 1536)
  AUTHORS   Sonmez,K., Zaveri,N.T., Kerman,I.A., Burke,S., Neal,C.R., Xie,X.,
            Watson,S.J. and Toll,L.
  TITLE     Evolutionary sequence modeling for discovery of peptide hormones
  JOURNAL   PLoS Comput Biol 5 (1), e1000258 (2009)
   PUBMED   19132080
REFERENCE   10 (bases 1 to 1536)
  AUTHORS   Mirabeau,O., Perlas,E., Severini,C., Audero,E., Gascuel,O.,
            Possenti,R., Birney,E., Rosenthal,N. and Gross,C.
  TITLE     Identification of novel peptide hormones in the human proteome by
            hidden Markov model screening
  JOURNAL   Genome Res 17 (3), 320-327 (2007)
   PUBMED   17284679
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC142413.4, AK043509.1, AK045625.1 and BG092156.1.
            
            On or before Jul 5, 2012 this sequence version replaced
            XM_908052.3, XM_620381.4.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849381,
                              SAMN00849383 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AC142413.4         42909-42924
            17-97               AC142413.4         43311-43391
            98-391              AK043509.1         11-304
            392-896             AK045625.1         486-990
            897-1526            AK043509.1         810-1439
            1527-1536           BG092156.1         1-10                c
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 74.03 cM"
     gene            1..1536
                     /gene="Spx"
                     /gene_synonym="B230216G23Rik; Npq"
                     /note="spexin hormone"
                     /db_xref="GeneID:319552"
                     /db_xref="MGI:MGI:2442262"
     exon            1..16
                     /gene="Spx"
                     /gene_synonym="B230216G23Rik; Npq"
                     /inference="alignment:Splign:2.1.0"
     CDS             11..367
                     /gene="Spx"
                     /gene_synonym="B230216G23Rik; Npq"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; neuropeptide Q"
                     /codon_start=1
                     /product="spexin isoform 1 precursor"
                     /protein_id="NP_001229274.1"
                     /db_xref="CCDS:CCDS85181.1"
                     /db_xref="GeneID:319552"
                     /db_xref="MGI:MGI:2442262"
                     /translation="MKGPSVLAVTAVVLLLVLSALENSSGAPQRLSEKRNWTPQAMLY
                     LKGAQGRRFLSDQSRRKELADRPPPERRNPDLELLTLPEAAALFLASLEKSQKGADEG
                     GNFDKSELLEDRLFNW"
     exon            17..97
                     /gene="Spx"
                     /gene_synonym="B230216G23Rik; Npq"
                     /inference="alignment:Splign:2.1.0"
     exon            98..155
                     /gene="Spx"
                     /gene_synonym="B230216G23Rik; Npq"
                     /inference="alignment:Splign:2.1.0"
     exon            156..218
                     /gene="Spx"
                     /gene_synonym="B230216G23Rik; Npq"
                     /inference="alignment:Splign:2.1.0"
     exon            219..308
                     /gene="Spx"
                     /gene_synonym="B230216G23Rik; Npq"
                     /inference="alignment:Splign:2.1.0"
     exon            309..1536
                     /gene="Spx"
                     /gene_synonym="B230216G23Rik; Npq"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1504..1509
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spx"
                     /gene_synonym="B230216G23Rik; Npq"
                     /note="hexamer: AATAAA"
     regulatory      1511..1516
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spx"
                     /gene_synonym="B230216G23Rik; Npq"
                     /note="hexamer: AATAAA"
     polyA_site      1527
                     /gene="Spx"
                     /gene_synonym="B230216G23Rik; Npq"
                     /note="major polyA site"
     polyA_site      1536
                     /gene="Spx"
                     /gene_synonym="B230216G23Rik; Npq"
                     /note="major polyA site"
ORIGIN      
        1 gggtcggaac atgaaggggc ccagcgtcct ggcagtgaca gccgtggtcc ttctcctggt
       61 gctgtctgcg ctggaaaact ccagcggtgc tccacagcga ctctctgaga agaggaactg
      121 gactccccaa gctatgctct atctgaaggg tgcacagggc cgccgcttcc tctccgacca
      181 gagccgtagg aaggagcttg cagaccggcc gcctccagaa agacgaaacc cagatcttga
      241 actgctgact ctcccagagg ctgcagccct gtttctggct tccttggaaa aatcacaaaa
      301 aggtgcagat gaaggaggga attttgataa aagcgaactc ttggaagaca gactcttcaa
      361 ctggtgaaaa cacaccagga caccttcaag tgtagtcctc gttgtgatgg aaaccagaac
      421 cacacgaggg gcccgtgcac ccttccttcc acccgagaaa cctgcacctc taggtttagt
      481 cccatggaaa tcctggctgg aacattacca ctggctggaa catcaccatt ggctggaaca
      541 tcacccatgt cttacgtagg cttggattat tcagggcctt tcacgtgtca aggctgagaa
      601 ctcagaaatg ttctttcttc tccctgcagt caggccctgc tttgattttg gccgtttgtt
      661 ttcttatccc ataattccct tgcctttggt gccactcaga agcaaatatc gctgtccctc
      721 atcgtcccag ccccagtgag agagagggtg caccgggaag ggaatggata ggatctccta
      781 gataggaact cgtctgcgag gtcaaataag gagggaggca aggagaagat gcctgataaa
      841 gctcccatga aaacacatct ctgtcgctgc tgaaacaggg caccatccat gatgctgaaa
      901 ctgctggaag gtctgtgaaa actgaggacc gggagcctct ttgtgctcct aggacctaac
      961 tgaactccac atagaagctt agaccttaag gttactgaca cacaaacaga tggtgatgtc
     1021 agagtttgct ggagcacacg gcacacaaag gtacaaacca gttgttcagg ttcagaggta
     1081 gtccatcttc tgtgactttg cattaaggta ttcccatgac acaagtgagt gccacatgca
     1141 tgatttaccc tttcccatgc acacacatgc acaggcacat gcacacgcac acagtgaagg
     1201 aatttgggag tgaaatccct ataccaagga gactaactta tttggaatta catcttgatc
     1261 accgtgtgtg tgtgtgtgtg tatgtgtgtg tgtgtgtgtg tgtgtttaaa aaaatattca
     1321 attcagagtc attaaccata agtgctcaag tgacaaagta gcgatactta ttgtccccag
     1381 cttacagtta ttaccttgaa ctatacagta tctttatgta aataccgctg taatctgata
     1441 ttttaactca actgtgttaa atcaaaacct agtgtatatg ttttgcctgc ttctcagttc
     1501 ataaataaac aataaaacta tattctagtt aatgta
//
