LOCUS       NM_001356507            2232 bp    mRNA    linear   ROD 18-FEB-2025
DEFINITION  Mus musculus serine palmitoyltransferase, long chain base subunit 3
            (Sptlc3), transcript variant 1, mRNA.
ACCESSION   NM_001356507 XM_006499295
VERSION     NM_001356507.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Montefusco,D., Jamil,M., Canals,D., Saligrama,S., Yue,Y.,
            Allegood,J. and Cowart,L.A.
  TITLE     SPTLC3 regulates plasma membrane sphingolipid composition to
            facilitate hepatic gluconeogenesis
  JOURNAL   Cell Rep 43 (12), 115054 (2024)
   PUBMED   39661520
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Kovilakath,A., Mauro,A.G., Valentine,Y.A., Raucci,F.J., Jamil,M.,
            Carter,C., Thompson,J., Chen,Q., Beutner,G., Yue,Y., Allegood,J.,
            Wang,X.X., Dail,J., Devarakonda,T., Myakala,K., Windle,J.J.,
            Subler,M.A., Montefusco,D., Willard,B., Javaheri,A., Bernas,T.,
            Mahata,S.K., Levi,M., Liu,J., Porter,G.A. Jr., Lesnefsky,E.J.,
            Salloum,F.N. and Cowart,L.A.
  TITLE     SPTLC3 Is Essential for Complex I Activity and Contributes to
            Ischemic Cardiomyopathy
  JOURNAL   Circulation 150 (8), 622-641 (2024)
   PUBMED   38660786
  REMARK    GeneRIF: SPTLC3 Is Essential for Complex I Activity and Contributes
            to Ischemic Cardiomyopathy.
REFERENCE   3  (bases 1 to 2232)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2232)
  AUTHORS   Oberbeck,N., Pham,V.C., Webster,J.D., Reja,R., Huang,C.S.,
            Zhang,Y., Roose-Girma,M., Warming,S., Li,Q., Birnberg,A., Wong,W.,
            Sandoval,W., Komuves,L.G., Yu,K., Dugger,D.L., Maltzman,A.,
            Newton,K. and Dixit,V.M.
  TITLE     The RIPK4-IRF6 signalling axis safeguards epidermal differentiation
            and barrier function
  JOURNAL   Nature 574 (7777), 249-253 (2019)
   PUBMED   31578523
REFERENCE   5  (bases 1 to 2232)
  AUTHORS   Li,S., Teegarden,A., Bauer,E.M., Choi,J., Messaddeq,N.,
            Hendrix,D.A., Ganguli-Indra,G., Leid,M. and Indra,A.K.
  TITLE     Transcription Factor CTIP1/ BCL11A Regulates Epidermal
            Differentiation and Lipid Metabolism During Skin Development
  JOURNAL   Sci Rep 7 (1), 13427 (2017)
   PUBMED   29044125
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2232)
  AUTHORS   Yoshimine,Y., Uto,H., Kumagai,K., Mawatari,S., Arima,S.,
            Ibusuki,R., Mera,K., Nosaki,T., Kanmura,S., Numata,M., Tamai,T.,
            Moriuchi,A., Tsubouchi,H. and Ido,A.
  TITLE     Hepatic expression of the Sptlc3 subunit of serine
            palmitoyltransferase is associated with the development of
            hepatocellular carcinoma in a mouse model of nonalcoholic
            steatohepatitis
  JOURNAL   Oncol Rep 33 (4), 1657-1666 (2015)
   PUBMED   25607821
  REMARK    GeneRIF: hepatic expression of Sptlc3 mRNA may be closely
            associated with NASH-associated hepatocarcinogenesis.
REFERENCE   7  (bases 1 to 2232)
  AUTHORS   Lau,C.G. and Marikawa,Y.
  TITLE     Morphology-based mammalian stem cell tests reveal potential
            developmental toxicity of donepezil
  JOURNAL   Mol Reprod Dev 81 (11), 994-1008 (2014)
   PUBMED   25269881
REFERENCE   8  (bases 1 to 2232)
  AUTHORS   Wang,Z., Kirkwood,J.S., Taylor,A.W., Stevens,J.F., Leid,M.,
            Ganguli-Indra,G. and Indra,A.K.
  TITLE     Transcription factor Ctip2 controls epidermal lipid metabolism and
            regulates expression of genes involved in sphingolipid biosynthesis
            during skin development
  JOURNAL   J Invest Dermatol 133 (3), 668-676 (2013)
   PUBMED   23096701
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL928899.10 and AK078679.1.
            
            On Oct 6, 2017 this sequence version replaced XM_006499295.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 both encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK054240.1, AK078679.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                AL928899.10        42357-42378
            23-2073             AK078679.1         1-2051
            2074-2232           AL928899.10        185347-185505
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 68.52 cM"
     gene            1..2232
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /note="serine palmitoyltransferase, long chain base
                     subunit 3"
                     /db_xref="GeneID:228677"
                     /db_xref="MGI:MGI:2444678"
     exon            1..410
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             294..1985
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /EC_number="2.3.1.50"
                     /note="LCB 3; LCB2b; SPT 3; serine-palmitoyl-CoA
                     transferase 3; long chain base biosynthesis protein 3;
                     long chain base biosynthesis protein 2b;
                     serine-palmityl-CoA transferase 3"
                     /codon_start=1
                     /product="serine palmitoyltransferase 3"
                     /protein_id="NP_001343436.1"
                     /db_xref="CCDS:CCDS16800.1"
                     /db_xref="GeneID:228677"
                     /db_xref="MGI:MGI:2444678"
                     /translation="MANLNDSAVTNGTLHNPKTQQGKRQSTGCVKNGISKEAQQNRKA
                     YAEDKPVFEPYQEAPLYVYVLTYMGYGIGILFGYLRDFMRNWGIEKCNAAVEREEQKD
                     FVPLYQDFENFYKRNLYMRIRDSWSHTVCSAPEPYMNVMEKVTDDYNWTFRHTGKVIE
                     NIINMASYNYLGLAGKYDDSMVRVKDTLEKYGVGVASTRNEMGTLDIHKELEDLMAEF
                     LNVEAVMSFGMGFATNAMNIPVFVGKGCLILSDEFNHTSVILGSRLSGAVIRPFKHNN
                     AENLEKLLREAIIRGQPGTGRAWKKILIVVEGVYSMEGSIVNLAQIVALKKKYKAYLY
                     IDEAHSIGCTGPTGRGVRELFGLDPEDIDVYMGTFTKSFSGSGGYIGGKKEIVDYLRM
                     QSHSTTYATSMSPVVAAQLIRSLKITMGYEGNIGGMERIQQLKENIKYFRRRLKEMGF
                     IIYGNDFSPVIPVLLYMPAKVSAFSRFLLKKKISVVVVGFPATSLPEGRARFSMSSAH
                     TREMLDTVLEVVDELGDLLNVKYFPLKKSGRAILYNKEGFDNEASFEEMHSEPEA"
     exon            411..596
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            597..751
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            752..900
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1025
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1119
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1225
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1445
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1572
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1708
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1709..1838
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1839..2232
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2208..2213
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /note="hexamer: AATACA"
     polyA_site      2232
                     /gene="Sptlc3"
                     /gene_synonym="C130053K05Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtctccaaat caccttagcc tcaaaaagca gctagcaggg taaactgtac tctcagaaac
       61 aggtttgttg ccatgcaaca cacaatgcca ctggagtaga ctagtaaaag gtaacagcta
      121 aagatctagt caatagatag gctgtggttc ttcttctgga aattcctgac cggttggatt
      181 cctttaaagg ctctgcccta cagtgcttta tgtcattact tcagttactt gaaaactgaa
      241 gttccaggag actgcagaaa gcgaacctgc cttggctcca gcacatccga ccaatggcca
      301 atctgaacga cagtgctgtt acaaatggga ctctacacaa tcctaagacc cagcaaggaa
      361 aaaggcaaag cactggttgt gtgaaaaatg gaatctcaaa ggaagcccag caaaatagga
      421 aggcatatgc tgaggataag ccagtctttg agccctacca ggaagcacca ctttatgtct
      481 acgttttaac ttacatggga tatggaattg gaatcctatt cggctatctc agagacttta
      541 tgagaaactg gggaattgaa aaatgcaatg cagctgtaga gcgagaagaa caaaaagact
      601 ttgtgccgct gtatcaggac tttgagaact tttataagag gaacctttac atgagaatca
      661 gagacagctg gagtcacacg gtctgcagtg ccccagagcc ttacatgaat gtcatggaga
      721 aagtgactga tgactataac tggacattca ggcatactgg aaaagttata gaaaatatca
      781 tcaacatggc ctcctacaac taccttgggc ttgcagggaa atatgatgat tcgatggtga
      841 gagttaagga tactttagaa aagtatggtg taggtgtggc cagcaccaga aatgaaatgg
      901 gtaccttgga tatacacaag gagttggagg atctcatggc tgagttcttg aatgtagaag
      961 cagtgatgtc ttttggaatg ggattcgcaa ccaacgcaat gaatatccct gtatttgttg
     1021 gaaagggatg cctcatttta agtgatgagt ttaaccacac ttcagtcatc ctaggatctc
     1081 gactctcagg tgcagtcata agacccttca aacacaataa tgcagaaaac ctagagaagc
     1141 tcctgagaga agctatcatc cgtggccagc ctggcacagg gagagcttgg aaaaagattc
     1201 tcatcgtggt agagggcgtc tacagcatgg aaggttccat tgtgaacctt gcccagatag
     1261 tggctctaaa gaagaaatac aaggcttacc tgtacattga tgaagctcac agtattggct
     1321 gtacaggccc cacgggacga ggcgtcagag agttatttgg actggaccct gaagatattg
     1381 acgtatacat gggtacattc accaagagct tctcgggctc aggaggatat ataggcggga
     1441 aaaaggaaat tgtggattat ttacggatgc agtcacatag cactacttat gctacatcca
     1501 tgagtcccgt agtagcagca caactcatca gatcattgaa gataactatg ggatatgagg
     1561 ggaacattgg aggtatggag agaatacagc aacttaagga aaacataaaa tacttccgac
     1621 ggcgactgaa agagatggga ttcatcatct atggcaatga tttctctccg gtgatccctg
     1681 tgctcctcta tatgcccgct aaagtgtctg ctttcagcag atttttgctt aagaaaaaga
     1741 tctcagtggt ggttgttgga tttccagcta cttccctgcc agaaggtcgg gccaggttca
     1801 gcatgtcctc tgcacatact cgggagatgt tagacacagt tttggaagtc gttgatgagc
     1861 tcggtgatct cctgaacgtg aaatatttcc cactcaagaa atcagggcgt gctatactct
     1921 acaataagga gggttttgac aatgaagcaa gctttgaaga gatgcactct gaaccggaag
     1981 cctaagttcc tgactccaaa tggaacataa agactgccag aaagacctcc ctccctcttt
     2041 atctcgaaag taatataatt gtattttacc ccttctgagg acaatttcat tttccaaaca
     2101 ggcttaattg gaaaagggaa ataatcattt atttaatgtc tcaagcctgc tgctacaaag
     2161 caaaataata taattctaga tttcagtttt ccctctcctt tgctataaat acacacatgc
     2221 gtacaaatgg ca
//
