LOCUS       NM_011479               6725 bp    mRNA    linear   ROD 07-AUG-2024
DEFINITION  Mus musculus serine palmitoyltransferase, long chain base subunit 2
            (Sptlc2), mRNA.
ACCESSION   NM_011479
VERSION     NM_011479.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   Lallement,J., Raho,I., Merlen,G., Rainteau,D., Croyal,M.,
            Schiffano,M., Kassis,N., Doignon,I., Soty,M., Lachkar,F.,
            Krempf,M., Van Hul,M., Cani,P.D., Foufelle,F., Amouyal,C., Le
            Stunff,H., Magnan,C., Tordjmann,T. and Cruciani-Guglielmacci,C.
  TITLE     Hepatic deletion of serine palmitoyl transferase 2 impairs
            ceramide/sphingomyelin balance, bile acids homeostasis and leads to
            liver damage in mice
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1868 (8), 159333 (2023)
   PUBMED   37224999
  REMARK    GeneRIF: Hepatic deletion of serine palmitoyl transferase 2 impairs
            ceramide/sphingomyelin balance, bile acids homeostasis and leads to
            liver damage in mice.
REFERENCE   3  (bases 1 to 6725)
  AUTHORS   Kim,G.T., Devi,S., Sharma,A., Cho,K.H., Kim,S.J., Kim,B.R.,
            Kwon,S.H. and Park,T.S.
  TITLE     Upregulation of the serine palmitoyltransferase subunit SPTLC2 by
            endoplasmic reticulum stress inhibits the hepatic insulin response
  JOURNAL   Exp Mol Med 54 (5), 573-584 (2022)
   PUBMED   35513574
  REMARK    GeneRIF: Upregulation of the serine palmitoyltransferase subunit
            SPTLC2 by endoplasmic reticulum stress inhibits the hepatic insulin
            response.
REFERENCE   4  (bases 1 to 6725)
  AUTHORS   Wang,T., Wang,Z., de Fabritus,L., Tao,J., Saied,E.M., Lee,H.J.,
            Ramazanov,B.R., Jackson,B., Burkhardt,D., Parker,M., Gleinich,A.S.,
            Wang,Z., Seo,D.E., Zhou,T., Xu,S., Alecu,I., Azadi,P., Arenz,C.,
            Hornemann,T., Krishnaswamy,S., van de Pavert,S.A., Kaech,S.M.,
            Ivanova,N.B. and Santori,F.R.
  TITLE     1-deoxysphingolipids bind to COUP-TF to modulate lymphatic and
            cardiac cell development
  JOURNAL   Dev Cell 56 (22), 3128-3145 (2021)
   PUBMED   34762852
REFERENCE   5  (bases 1 to 6725)
  AUTHORS   Alatibi,K.I., Wehbe,Z., Spiekerkoetter,U. and Tucci,S.
  TITLE     Sex-specific perturbation of complex lipids in response to
            medium-chain fatty acids in very long-chain acyl-CoA dehydrogenase
            deficiency
  JOURNAL   FEBS J 287 (16), 3511-3525 (2020)
   PUBMED   31971349
REFERENCE   6  (bases 1 to 6725)
  AUTHORS   Hanada,K., Hara,T., Nishijima,M., Kuge,O., Dickson,R.C. and
            Nagiec,M.M.
  TITLE     A mammalian homolog of the yeast LCB1 encodes a component of serine
            palmitoyltransferase, the enzyme catalyzing the first step in
            sphingolipid synthesis
  JOURNAL   J Biol Chem 272 (51), 32108-32114 (1997)
   PUBMED   9405408
REFERENCE   7  (bases 1 to 6725)
  AUTHORS   Weiss,B. and Stoffel,W.
  TITLE     Human and murine serine-palmitoyl-CoA transferase--cloning,
            expression and characterization of the key enzyme in sphingolipid
            synthesis
  JOURNAL   Eur J Biochem 249 (1), 239-247 (1997)
   PUBMED   9363775
REFERENCE   8  (bases 1 to 6725)
  AUTHORS   Nagiec,M.M., Lester,R.L. and Dickson,R.C.
  TITLE     Sphingolipid synthesis: identification and characterization of
            mammalian cDNAs encoding the Lcb2 subunit of serine
            palmitoyltransferase
  JOURNAL   Gene 177 (1-2), 237-241 (1996)
   PUBMED   8921873
REFERENCE   9  (bases 1 to 6725)
  AUTHORS   Kerr,S.M., Vambrie,S., McKay,S.J. and Cooke,H.J.
  TITLE     Analysis of cDNA sequences from mouse testis
  JOURNAL   Mamm Genome 5 (9), 557-565 (1994)
   PUBMED   8000140
REFERENCE   10 (bases 1 to 6725)
  AUTHORS   Mandon,E.C., Ehses,I., Rother,J., van Echten,G. and Sandhoff,K.
  TITLE     Subcellular localization and membrane topology of serine
            palmitoyltransferase, 3-dehydrosphinganine reductase, and
            sphinganine N-acyltransferase in mouse liver
  JOURNAL   J Biol Chem 267 (16), 11144-11148 (1992)
   PUBMED   1317856
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CT030249.7, BY789607.1,
            AK044917.1, AK037908.1 and CF747116.1.
            
            On Sep 16, 2015 this sequence version replaced NM_011479.3.
            
            Summary: This gene encodes a long chain base subunit of serine
            palmitoyltransferase. The enzyme, serine palmitoyltransferase,
            consists of two different subunits, and is the key enzyme in
            sphingolipid biosynthesis. It catalyzes the
            pyridoxal-5-prime-phosphate-dependent condensation of L-serine and
            palmitoyl-CoA to 3-oxosphinganine. A mutant allele of this gene in
            mice is used as a model for the human disease 'Susceptibilty to
            Psoriasis 1'. Mutations in the human gene are associated with
            hereditary sensory neuropathy type I. [provided by RefSeq, Sep
            2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK044917.1, ERR3363658.4910376.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                CT030249.7         91704-91723         c
            21-125              BY789607.1         21-125
            126-3879            AK044917.1         2-3755
            3880-6185           AK037908.1         1535-3840
            6186-6725           CF747116.1         1-540
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 41.52 cM"
     gene            1..6725
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /note="serine palmitoyltransferase, long chain base
                     subunit 2"
                     /db_xref="GeneID:20773"
                     /db_xref="MGI:MGI:108074"
     exon            1..310
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /inference="alignment:Splign:2.1.0"
     CDS             185..1867
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /EC_number="2.3.1.50"
                     /note="LCB 2; SPT 2; serine-palmitoyl-CoA transferase 2;
                     long chain base biosynthesis protein 2; long chain base
                     biosynthesis protein 2a"
                     /codon_start=1
                     /product="serine palmitoyltransferase 2"
                     /protein_id="NP_035609.1"
                     /db_xref="CCDS:CCDS26076.1"
                     /db_xref="GeneID:20773"
                     /db_xref="MGI:MGI:108074"
                     /translation="MRPEPGGCCCRRPMRANGCVKNGEVRNGYLRSSTATVAAAGQIH
                     HVTENGGLYKRPFNEAFEETPMLVAVLTYVGYGVLTLFGYLRDFLRHWRIEKCHHATE
                     REEQKDFVSLYQDFENFYTRNLYMRIRDNWNRPICSVPGAKVDIMERKSHDYNWSFKY
                     TGNIIKGVINMGSYNYLGFARNTGSCQEAAAEVLKEYGAGVCSTRQEIGNLDKHEELE
                     KLVARFLGVEAAMTYGMGFATNSMNIPALVGKGCLILSDELNHASLVLGARLSGATIR
                     IFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKK
                     YKAYLYLDEAHSIGALGPSGRGVVDYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKEL
                     IDYLRTHSHSAVYATSMSPPVMEQIITSMKCIMGQDGTSLGKECIQQLAENTRYFRRR
                     LKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARF
                     CLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPFDETTYEETED"
     exon            311..505
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /inference="alignment:Splign:2.1.0"
     exon            506..660
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /inference="alignment:Splign:2.1.0"
     exon            661..809
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /inference="alignment:Splign:2.1.0"
     exon            810..934
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1028
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1134
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1354
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1481
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1617
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1618..1747
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1748..6710
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2111..2116
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /note="hexamer: ATTAAA"
     polyA_site      2136
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /note="major polyA site"
     regulatory      6690..6695
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
                     /note="hexamer: AATAAA"
     polyA_site      6710
                     /gene="Sptlc2"
                     /gene_synonym="LCB2; LCB2a; Spt2"
ORIGIN      
        1 ggctgccggg cttcggcgag ccccgtcttc tcgggtggcc ccgccctcgc gtgacggccg
       61 gaccgggaat tggcagcggc gctgcagcca tttccggttt cggagaggtg ggtgcggtgc
      121 ggagcgggag ttggctccgc cgccacctcc agagcctgtc ctgctgccgg gtgctgccgg
      181 gaggatgcgg ccggagcccg gaggctgctg ctgccgccgc ccgatgcggg cgaacggctg
      241 cgtcaagaac ggggaagtga ggaacgggta cttgaggagc agcaccgcca ccgtcgcggc
      301 tgccggccag attcatcatg taacagaaaa tggaggactg tacaaaagac cgtttaatga
      361 agcttttgaa gaaacaccca tgctggttgc tgtgctcaca tatgtgggct atggcgtact
      421 caccctcttt ggatatcttc gagatttctt gaggcattgg agaattgaaa agtgccacca
      481 tgcaacagaa agagaagaac aaaaggactt tgtgtccttg tatcaggatt ttgaaaactt
      541 ctatacaagg aacctctaca tgagaatcag agacaactgg aatcgaccta tctgtagtgt
      601 gcctggagcc aaggtggata tcatggagag aaaatctcat gactataact ggtcattcaa
      661 gtacacaggg aatataatta aaggtgtaat aaacatgggt tcctacaact atcttggatt
      721 tgcgaggaac actggatcat gtcaggaagc agctgctgaa gtcctcaagg agtatggagc
      781 aggggtgtgc agcactcgtc aggaaattgg aaacctggac aagcatgaag aactagagaa
      841 attggtagca aggttcttag gtgtggaagc tgctatgacc tatggcatgg gatttgcaac
      901 aaattcaatg aacattcctg ctcttgttgg caaaggttgc ctgattctga gtgatgagct
      961 gaaccatgcg tcactggttc taggagccag actgtcagga gcaaccattc gaatcttcaa
     1021 acacaacaat atgcaaagct tagagaagct tttaaaagat gccattgttt atggtcagcc
     1081 tcggacaaga agaccctgga agaaaatctt aatccttgtg gaaggcatat atagtatgga
     1141 ggggtctatt gttcgccttc ctgaagtgat tgctctcaag aagaaataca aggcatactt
     1201 gtatctggat gaggctcaca gcattggggc tcttggccct tcagggcgag gcgtggtaga
     1261 ttactttggc ctggatcctg aggatgtaga tgttatgatg ggaacattca caaagagctt
     1321 cggtgcttca ggaggataca tcggaggcaa gaaggagctg atagactacc tgcgcacaca
     1381 ttctcacagt gctgtgtatg ccacgtcgat gtcaccgcct gtgatggaac agattatcac
     1441 ctccatgaag tgcatcatgg ggcaggatgg caccagtctt ggcaaagaat gtatacagca
     1501 gttggctgag aacaccaggt atttcaggag acgcctgaag gaaatggggt tcatcatcta
     1561 tggcaatgaa gactccccgg tggtgccttt gatgctctac atgccggcca aaattggcgc
     1621 ctttggaaga gagatgctga agcggaacat tggtgtagtt gtggtgggat ttcctgctac
     1681 cccgatcatt gagtccagag ccagattttg cctgtcagca gctcatacca aagaaatact
     1741 tgacactgct ttgaaggaga tagatgaagt tggggatctg ctgcagctaa agtactctcg
     1801 ccaccggctg gtgcctctac tggacaggcc ctttgatgag actacctatg aagagacaga
     1861 agactgagcc tttctggtgc tccctagagg gggataattc ctcccaggac agtgtgtggc
     1921 ctttctgagc caattccagg aaccacactt cagtgaccac ttcatgtgaa agacatttct
     1981 gaagctactg aaggtggcca ccttcactcc aaatggcatt ttgtaaatag taaaaaaacc
     2041 aaactgcttc acattcttcc tctgtttgct aagtctcaca cacacacaca cacatatgat
     2101 tactttgtcc attaaaaatg ttttatttta taactattct acttgtgaaa tcacgctgac
     2161 ccttgcctgt ggcttctgga acaggcattc cccgctgcag tcacctcata ggcttggatc
     2221 catcaagacc tacttcaggc cccggctctc caaccgagag agactcactg gccctctccc
     2281 tagggagcaa gtgccttctg ctgactttcc gcccaggtct gaggttgccc cccttgtaat
     2341 ccctatttaa ccagtcaagt caatcaacca aagcactctg tctttagtct taaggtaaat
     2401 gttaccaagg ccagaaggct tatgtgactc cccgtggcca aggacctcct aagactggtc
     2461 tctctctttc tgttctgctc accaaataat ttattcatta tcagagcttt cttcatatgg
     2521 tcggtcatag gctgaagatg tatccaacgt ttaggttctc cctgagatct aagcctaatg
     2581 gttcccacct ctaaagttac gacagcctct tgctggttct gggtccaggt taagatttga
     2641 cccttaaagg caagtgttgg tcaatctttt gctcatgttc cacttcacca ttagtggtat
     2701 aagttttccg cgtagacgct gacccacctg aacagtgctc ttccaaaggc caattctcct
     2761 cctcctcctg ggctaggaag attttgccca gcacctgggg ctggcttatc ttgtgcttcc
     2821 attgtatttt tccccctttg aggaattctg atcaaatacc tctttcttaa actggaatag
     2881 cctgttttcc tgtcaccgcg gggtcaggga cttcattcag agctttgtgc tgcttgacca
     2941 aagacacatc tgaagagcac cattttaaag ttttgttttt tttcttttga ccaaagctta
     3001 attgaagaga acttttggct ttatttattg tgctgaagat gaatttatct tttagtagca
     3061 atcacgtgtt agaaggaagg actgagaaca gaatgggggg aaaaactatt tagaagtaca
     3121 ttttgtatgc cattctttaa aatgattctt attactgata aagatcccaa accaagtcct
     3181 tctgaggacc tgtcagctgc agtacaagga caagaagaga ggcagtgtca tttgtgaagc
     3241 tctgttttca tgccctggat ctgttatctt tggccctctt ggcatctgtg agcttttagc
     3301 ccttggcatg ttctaaagtt gtatgtgagt tttttaaaga gtagaaatgg gtgaacaggc
     3361 tgggtgtggt aacctatgcc tttaatccta tatctcagaa ggcagaggca ggctgatctc
     3421 agtttgaggt cagcctggtc tgcatagtga aacctgaaga gggagacaga gaataaaaag
     3481 attccataac taagtcctat aaagctcaaa taccttcctt cctcacattc catcttagaa
     3541 tcccactgga gtactaagta ttttgcacag cacttactat ttaatgatgc agacctaata
     3601 gggcttgcaa aagtaacaag ccttaagcag tgaacgcgcc tgctagtctt ttagctcaca
     3661 tgaaatcact tcaaattaaa atgctcttac gggcatctaa atttttctgt atgtatctct
     3721 tggtgcaaag tttattttgg gctaaacgta actaagttaa agatttttgt tattgtttgt
     3781 cgggttggtt gggttttttg ttttgttttg aagagacaaa acccctgtgt tctacccttg
     3841 atctctactt ccgagaggac aaaatggttt atacacttta tatgtggctg tttttttgta
     3901 aatctgtaat agaaatctct ggcccaatta tgtcataagt gactataatc caaaaatcat
     3961 agtgaataga gatagtgaat agagacctgg gccctaaata gatagattca ggcattccta
     4021 agtcagccat caccaccaaa gccgactgta ctttgcctca tgcttttcat ttagcattgt
     4081 ccttactcct gtgtttgtaa gacccacttc acagcccacg tcaggaacca gaagataatc
     4141 agaatcatgc agaagatttg gcccgagggg caagggcagg gaataaatat ctcctcttca
     4201 tagtaacctt gtttaaagga tctagccttg gagctgaaga gagggcttag tggttgggga
     4261 cactgacttc tttttcagag gacccgggtt caaatctcag cacccacggg gtagcccaca
     4321 actgtctgta actccaagat ttgatgctct cacatagaca tacatgtagg caaaatacat
     4381 aaactaaagg taaataaata aaacttggtg ggaaaaaaat aaccagcccc cttccaccct
     4441 tcattcacag tcttcacttc tgactcaaac cctctcgtag gctgaagggt agagcgtcct
     4501 cagagagatc tgcctctgag cctggactga cattctggat tctgcctcca gaggacactg
     4561 acaagtgctg gtgcaccata tggggagctg tagggcagac aacctgctcc ttgcatgcac
     4621 aaccatgcac tttggtgttt cttcccttat tagttaaggg tatccatatc tgagtctctg
     4681 atgactggca aggtatatag ccacattacc tggtgtgtaa tatatcttgc aaaaagcaag
     4741 ataaacatct ggcttagatt tcttgacttt agacaaaggg ggaaatgata cctgctacca
     4801 cctgtttttc caaactgatg aatctaccta attgcagtat tttgacaaaa cccatagaaa
     4861 tgtaaataaa tggttgctgt gggatcaagt atggtacaca cctgtaatct cagaaggcag
     4921 aagcaagcag atctagaatt ataaattatt ctgtgttaca aagtgagact ctacagaagg
     4981 aacaaaaaga gagtggacta ttgctatgac ttaatgtcag gacgctgttc tcccagtgtt
     5041 ctaggtaaga cacagctgcg tgttcgtgcc tctctgcttc caacatgggg ttagttaaga
     5101 aaaggagagc agtaggcttt cctctcttcc tgtgccaagc tgatcaggag ctgtcaagta
     5161 tttctcacaa ataaaaccac gttagcctgg ggctatgaag ggggagttat taaaatcggg
     5221 ggttagttaa gaaaaggaga gcagtaggct ttcctctctt cctgtgccaa gctgatcagg
     5281 agctgtcaag tatttctcac aaataaaacc acgttagcct ggggctatga agggggagtt
     5341 attaaaatcg ggattctgct tggcagttta tcaaagaata tatagtgcct ccaccccacc
     5401 ccctccagtt ccagaaccta tggataacct cttacctgct tactagtata ctcagtaccc
     5461 agcaggcatc ttaatccatt aagctggtta atcaaaacac cttccaagtg gagaaaagca
     5521 tatccttcct cccctctgcc ttatccacgt gaagtgtgtg ctcgggcttc tagccagcgc
     5581 acagggaaca gagggaggcc caccagggat cctggcttgc ctaccgaagg ctattgctgc
     5641 tgtagttttc agtgacttct gtcttctaag atgctttttc ctgtagattg aaaggtttct
     5701 aggaagaaaa caaacgggaa cttgcttagg ccctaatgat gagcgttttc ctctttgtgg
     5761 agtctgaagg ctcacattca cagtttaccc ttaggagcag cctggtgggc gggagctgtg
     5821 agcttgtttg ctacgatgtg gccttaaaac tgttactgtt tccagtgtga tgaattctaa
     5881 taagtcaacc ctgaagaatg ctgtggctct tgtcccgtgt cactgctttt gtgtaaagct
     5941 ttgttctttg ggagcagtta ttggagacgg aatgaaaggt gcttgcaaga acagcaccct
     6001 gatgtgctca gtgtctgttt cctgcgccct ctgcactctt tccgtgtgca aggaactcca
     6061 gttaactcaa agtccagcac aggtcccagg taattcgtgt acacatcctg agtagagaat
     6121 gtaccaaggg tggtggagtt atcgtccccc ttaacactgt atgtcaggat tagtttagcc
     6181 aatagttgga attaagagct tccttactag tttgttcttc acaggacata gaaacctctc
     6241 ctgtttgttt agcctgcatg cccacagtct tgatgatccc tctctgtaca aaggcagcta
     6301 acaataaggg acgggggaag agaagagaac aggtgtctgt gggagagttg aattagagtg
     6361 atcttgtaga tccacgtttt cccactgacc aagttactgt aggctggagt ctgcaaggtg
     6421 tactgtgtcg tgaacccttt tcattcttgt aatgttctta tcatctggga atgtgagatt
     6481 tttaatttta ggaataatct tacaaataat cactcttctg tagccgtctt gccagatgtt
     6541 ttatttttct ttattttatt ttattttata ttggatgact gtgaagctat acatagtagg
     6601 ttatggagga tatcaatttt cagagtgcat gttaagaaat aaagcgaacc tacttgagat
     6661 gaatcatccc ataaatattg ttttgcccta ataaaatatt cagaaataga aaaaaaaaaa
     6721 aaaaa
//
