LOCUS       NM_032610               8737 bp    mRNA    linear   ROD 21-AUG-2024
DEFINITION  Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript
            variant sigma1, mRNA.
ACCESSION   NM_032610
VERSION     NM_032610.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Kwak,E.A., Ahmed,T., Flores,P.C., Ortiz,H.R., Langlais,P.R.,
            Mythreye,K. and Lee,N.Y.
  TITLE     Beta IV spectrin inhibits the metastatic growth of melanoma by
            suppressing VEGFR2-driven tumor angiogenesis
  JOURNAL   Cancer Med 12 (18), 18981-18987 (2023)
   PUBMED   37680049
  REMARK    GeneRIF: Beta IV spectrin inhibits the metastatic growth of
            melanoma by suppressing VEGFR2-driven tumor angiogenesis.
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   Ahmed,T., Ramonett,A., Kwak,E.A., Kumar,S., Flores,P.C.,
            Ortiz,H.R., Langlais,P.R., Hund,T.J., Mythreye,K. and Lee,N.Y.
  TITLE     Endothelial tip/stalk cell selection requires BMP9-induced
            betaIV-spectrin expression during sprouting angiogenesis
  JOURNAL   Mol Biol Cell 34 (7), ar72 (2023)
   PUBMED   37126382
  REMARK    GeneRIF: Endothelial tip/stalk cell selection requires BMP9-induced
            betaIV-spectrin expression during sprouting angiogenesis.
REFERENCE   3  (bases 1 to 8737)
  AUTHORS   Kurki,S.N., Uvarov,P., Pospelov,A.S., Trontti,K., Hubner,A.K.,
            Srinivasan,R., Watanabe,M., Hovatta,I., Hubner,C.A., Kaila,K. and
            Virtanen,M.A.
  TITLE     Expression patterns of NKCC1 in neurons and non-neuronal cells
            during cortico-hippocampal development
  JOURNAL   Cereb Cortex 33 (10), 5906-5923 (2023)
   PUBMED   36573432
REFERENCE   4  (bases 1 to 8737)
  AUTHORS   Nassal,D.M., Shaheen,R., Patel,N.J., Yu,J., Leahy,N., Bibidakis,D.,
            Parinandi,N.L. and Hund,T.J.
  TITLE     Spectrin-Based Regulation of Cardiac Fibroblast Cell-Cell
            Communication
  JOURNAL   Cells 12 (5), 748 (2023)
   PUBMED   36899883
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8737)
  AUTHORS   Teliska,L.H., Dalla Costa,I., Sert,O., Twiss,J.L. and Rasband,M.N.
  TITLE     Axon Initial Segments Are Required for Efficient Motor Neuron Axon
            Regeneration and Functional Recovery of Synapses
  JOURNAL   J Neurosci 42 (43), 8054-8065 (2022)
   PUBMED   36096668
REFERENCE   6  (bases 1 to 8737)
  AUTHORS   Bronson,R.T., Sweet,H.O., Spencer,C.A. and Davisson,M.T.
  TITLE     Genetic and age related models of neurodegeneration in mice:
            dystrophic axons
  JOURNAL   J Neurogenet 8 (2), 71-83 (1992)
   PUBMED   1634998
REFERENCE   7  (bases 1 to 8737)
  AUTHORS   Rogers,H.W., Sheehan,K.C., Brunt,L.M., Dower,S.K., Unanue,E.R. and
            Schreiber,R.D.
  TITLE     Interleukin 1 participates in the development of anti-Listeria
            responses in normal and SCID mice
  JOURNAL   Proc Natl Acad Sci U S A 89 (3), 1011-1015 (1992)
   PUBMED   1531270
REFERENCE   8  (bases 1 to 8737)
  AUTHORS   Litt,M., Luty,J., Kwak,M., Allen,L., Magenis,R.E. and Mandel,G.
  TITLE     Localization of a human brain sodium channel gene (SCN2A) to
            chromosome 2
  JOURNAL   Genomics 5 (2), 204-208 (1989)
   PUBMED   2571571
REFERENCE   9  (bases 1 to 8737)
  AUTHORS   Horner,K.C., Lenoir,M. and Bock,G.R.
  TITLE     Distortion product otoacoustic emissions in hearing-impaired mutant
            mice
  JOURNAL   J Acoust Soc Am 78 (5), 1603-1611 (1985)
   PUBMED   4067076
REFERENCE   10 (bases 1 to 8737)
  AUTHORS   Horner,K.C. and Bock,G.R.
  TITLE     Combined electrophysiological and autoradiographic delimitation of
            retrocochlear dysfunction in a mouse mutant
  JOURNAL   Brain Res 331 (2), 217-223 (1985)
   PUBMED   3986566
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK147558.1, AB055618.1 and AC157561.10.
            
            On Oct 14, 2006 this sequence version replaced NM_032610.1.
            
            Transcript Variant: This variant (sigma1) represents the longest
            transcript and encodes the longer isoform (sigma1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB055618.1, AK147558.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-807               AK147558.1         12-818
            808-5604            AB055618.1         808-5604
            5605-7401           AK147558.1         5618-7414
            7402-7542           AB055618.1         7402-7542
            7543-7626           AC157561.10        186276-186359       c
            7627-7758           AB055618.1         7627-7758
            7759-8737           AC157561.10        182724-183702       c
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 15.88 cM"
     gene            1..8737
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /note="spectrin beta, non-erythrocytic 4"
                     /db_xref="GeneID:80297"
                     /db_xref="MGI:MGI:1890574"
     exon            1..127
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            128..314
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..7828
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /note="isoform sigma1 is encoded by transcript variant
                     sigma1; beta-spectrin 4; lumbosacral neuroaxonal
                     dystrophy; spectrin beta 4; quivering"
                     /codon_start=1
                     /product="spectrin beta chain, non-erythrocytic 4 isoform
                     sigma1"
                     /protein_id="NP_115999.2"
                     /db_xref="CCDS:CCDS21019.1"
                     /db_xref="GeneID:80297"
                     /db_xref="MGI:MGI:1890574"
                     /translation="MAQVPGEVDNMEGPAVSNNNNPSARWESPDRGWDREPPAAANAA
                     ASLFECSRIKALADEREAVQKKTFTKWVNSHLARVGCHIGDLYADLRDGFVLTRLLEV
                     LSGEQLPRPTRGRMRIHSLENVDKALQFLKEQRVHLENVGSHDIVDGNHRLTLGLVWT
                     IILRFQIQVIKIETEDNRETRSAKDALLLWCQMKTAGYPEVNIQNFTTSWRDGLAFNA
                     LIHRHRPDLVDLSKLTKSNANYNLQRAFRTAEQHLGLARLLDPEDVNMEAPDEKSIIT
                     YVVSFYHYFSKMKALAVEGKRIGKVLDQVLEVDKIIERYEELAAELLAWIHRTVDLIS
                     NQKFANSLSGVQQQLQAFTAYCTLEKPVKFQEKGNLEVLLFSIQSKLRAHNRRLFVPR
                     EGCGIWDIDKAWGELEKAEHEREAALRAELIRQEKLELLAQRFDHKVAMRESWLNENQ
                     RLVSQDNFGYELPAVEAAMKKHEAIEADIAAYEERVQGVAELAQALAAEGYYDARRVA
                     AQRDSVLRQWALLTGLVGARRTRLEQNLALQKVFQEMVYMVDWMEEMQTQLLSRECGQ
                     HLVEADDLLQKHGLLEGDIAAQSERVEALNAAALRFSQLQGYQPCDPQVICNRVNHVH
                     GCLSELQEQAARRRAELEASRSLWALLQELEEAESWARDKERLLEATSGSGGAAGTAG
                     GAHDLSSTARLLAQHKILQGELGGRRALLQQALRRGEELAAAGGSVGPGAEPLHLAGL
                     AERAASARRRWQRLEEAAARRERRLQEARALHQFGADLDGLLDWLRDAYRLAAAGDFG
                     HDEASSRRLARQHRALTGEVEAHRGPVGGLRRQLATLGGASGAGPLVVALQVRVVEAE
                     QLFAEVTEVAALRRQWLRDALAVYRMFGEVHACELWIGEKEQWLLAMRVPDSLDDVEV
                     VQHRFESLDQEMNSLMGRVLDVNQTVQELVEGGHPSSDEVRSCQDHLNSRWNRIVELV
                     EQRKEEMSAVLLVENHVLEVAEVRAQVREKRRAVESAPRAGGALQWRLSGLEAALQAL
                     EPRQAALLEEAALLAERFPAQATRLHQGAEELGAEWGALAGAAQACGEAVAAAGRLQR
                     FLRDLDTFLDWLVRAQEAAGAVEGPLPRSLEEADGLLARHAALKEEVDQREEDYARIV
                     AASEALLASEGAELGPGLALDEWLPHLEVGWHKLLGLWEERREALVQAHVYQLFLRDL
                     CQALAVLRNQEVALSGAELPCTVESVEEAMKRHRDFLTTMELNQQKMQVAVQAAESLL
                     RQGNAYGEQAQEAVARLLEKSQENQLRAQQWMQKLLDQLVLQHFLRDCHELDGWIHEK
                     MLMARDGTREDSHKLHKRWLRHQAFMAELAQNKEWLEKIEREGQQLMQEKPELAASVR
                     KKLGEIRQCWAELESTTQAKARQLFEASKADQLVQSFAELDKRLLHMESQLQDVDPGG
                     DLATVNSQLKKLQSMESQVEEWCREVGELQAQTAALPLEQASKELVGERQSAVGERLV
                     RLLEPLQERRRLLLASKELHQVAHDLDDELAWVQERLPLAMQTERGTGLQAVQQHIKK
                     NQGLRREIQAHGPRLEEVLERAGVLASLRSPEAEAVRRGQEQLQSAWTGLREAAERRQ
                     QTLDAAFQVEQYYFDVAEVEAWLGEQELLMMSEDKGKDEQSTLQLLKKHLQLEQGVEN
                     YEESIAQLSRQCRALLEMGHPDSEQISRRQSQVDRLYVALKELGEERRVSLEQQYWLY
                     QLSRQVDELEHWIAEKEVVAGSPELGQDFEHVSVLQEKFSEFASETGTAGRERLAAVN
                     QMVDELIECGHTAAATMAEWKDGLNEAWAELLELMGTRAQLLAASRELHKFFSDAREL
                     QGQIEEKRRRLPRLTAPPEPRPSASSMQRTLRAFEHDLQLLVSQVRQLQEGAAQLRTV
                     YAGEHAEAIASREQEVLQGWKELLAACEDARLHVSSTADALRFHSQARDLLSWMDGIA
                     GQIGAADKPRDVSSVEVLMNYHQGLKTELEARVPELATCQELGRSLLLNKSAMADEIQ
                     AQLDKLGSRKEEVSEKWDRHWEWLQQMLEVHQFAQEAVVADAWLTAQEPLLQSRELGS
                     SVDEVEQLIRRHEAFRKAAAAWEERFSSLRRLTTIEKLKAEQSKQPPTPLLGRKFFGD
                     PTELAAKAAPLLRPGGYDRGLEPLARRASDTLSAEVRTRVGYVRQELKPERLQPRIDR
                     LPETSGKVEPAAPTAAALDTTDTPGTPAATELVRPRSERQELADRAEELPRRRRSERQ
                     ESVDQPEETARRRRPERQESADHEGPHSLTLGRYEQMERRRERRERRIERQESSEQET
                     PTRGELVKGKATLADIVEQLQEKEAGPGIPAGVPSLPQPRELPPGRLPNGLEPPERTP
                     RPDRPRARDRPKPRRRPRPREGGEGGGSRRSRSAPAQGGSAPAPPPPPTHTVQHEGFL
                     LRKRELDANRKSSNRSWVSLYCVLSKGELGFYKDSKGPASGGTHGGEPLLSLHKATSE
                     VASDYKKKKHVFKLQTQDGSEFLLQAKDEEEMNGWLEAVANSVAEHAEIARWGQTLPT
                     TSSTDEGNPKREGGERRASGRRK"
     exon            315..466
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            467..640
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            641..732
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            733..813
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            814..929
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1042
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1230
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1348
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1507
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1810
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..1961
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            1962..2895
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            2896..3033
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            3034..3784
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            3785..3987
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            3988..4078
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            4079..4225
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            4226..4489
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            4490..4714
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            4715..4804
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            4805..5083
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            5084..5214
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            5215..5419
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            5420..5800
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            5801..6045
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            6046..6184
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            6185..6269
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            6270..6466
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            6467..7086
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            7087..7159
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            7160..7452
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            7453..7626
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            7627..7669
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     exon            7670..8737
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8713..8718
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /note="hexamer: AATAAA"
     polyA_site      8737
                     /gene="Sptbn4"
                     /gene_synonym="1700022P15Rik; 5830426A08Rik; dyn; lnd;
                     nmf261; nmf379; qv; ROSA62; SpbIV; Spnb4"
                     /note="major polyA site"
ORIGIN      
        1 gcggatctgg ccgagcggcg ggccggtggg gccgaacaga gccgggcccg gcccggcgac
       61 gaacgcccgc agggagggcg gtgctgcgcg gcgagcatcc gggaccgcat cggcggccgg
      121 cgcccaggcc ctgcctcccc caatggcaca agtaccaggg gaggtggaca acatggaggg
      181 cccagcagtc tccaacaaca acaacccttc agctcgctgg gagagcccag atcggggctg
      241 ggatcgggaa cctcccgcag ccgccaatgc tgcggcttca ctctttgagt gctctcggat
      301 caaggcttta gcagatgagc gagaggctgt gcagaagaaa accttcacca agtgggtgaa
      361 ttcgcacctt gcccgcgtgg gctgccacat tggagacctg tatgcagacc ttcgggacgg
      421 gtttgtcctc acccggcttc tggaagtgct gtcgggggag cagctgccga ggcccactcg
      481 cggccgcatg cggatccact cgctggagaa cgtggacaag gctctgcagt tcctgaagga
      541 gcagcgtgtg catctggaga acgtggggtc acatgacatc gtggatggga accaccgact
      601 gacgctgggg ctggtctgga ccatcatcct gcgcttccag attcaagtca tcaagattga
      661 gacagaagac aacagagaga cccgctccgc caaggacgct ctgctcttgt ggtgccagat
      721 gaaaacagcc ggttaccctg aggtaaacat ccagaatttc accaccagct ggagagatgg
      781 cctggccttc aatgccctca ttcaccgtca caggcctgat cttgtggacc tcagcaaact
      841 taccaagtcc aacgccaact acaacctgca gagagctttc cgcacagccg agcagcacct
      901 ggggctagca cgtctgctgg accccgaaga tgtgaacatg gaggctccag atgagaagtc
      961 cattatcacc tatgtggtct ccttctacca ctacttctcc aagatgaagg ctctggcggt
     1021 ggagggaaaa cggattggaa aggtcctgga ccaggtgttg gaggtggaca agatcataga
     1081 acgctatgag gagctggcgg cggagctgtt ggcctggatc catcgcactg tggacctcat
     1141 tagcaaccaa aagtttgcta actcactgag tggggtgcag cagcagctgc aggccttcac
     1201 ggcttactgt acgctggaga agcccgtcaa gtttcaggag aaggggaacc tggaggtgct
     1261 actcttcagc atccaaagca aactgcgcgc ccacaaccgg cgcctctttg tgcctcgaga
     1321 aggctgtggc atctgggata ttgacaaggc gtggggtgag ctggagaagg cagagcatga
     1381 gcgagaggcg gccctcagag ctgagctcat ccggcaggag aagttggaac tcttggctca
     1441 aagatttgac cacaaggtgg ccatgaggga gagctggcta aatgagaacc agcgcctggt
     1501 ctcccaggac aacttcggat atgagctgcc ggcagtggag gcagccatga agaagcacga
     1561 agccattgag gcagacattg cagcctacga ggagcgggtt cagggtgtgg cggagttagc
     1621 ccaggcgttg gcagctgaag gctactatga tgcccggcgg gtggctgccc agcgtgacag
     1681 tgtccttcgc cagtgggccc tgctgaccgg gctggtaggt gcccggcgga cccggcttga
     1741 gcagaatctg gccctgcaga aggtcttcca ggagatggtc tacatggtgg actggatgga
     1801 ggagatgcag actcagctgc tgtctcggga atgtgggcag cacctggtgg aggccgatga
     1861 cctgctgcag aagcacgggc tgctggaggg ggacatcgca gcccagagtg agcgagtgga
     1921 ggccctcaat gctgcagccc tgagattctc ccagctacag ggctatcagc cctgcgaccc
     1981 tcaggtcatt tgcaaccgtg tgaaccacgt gcatggctgt ctgtcggagc tgcaggagca
     2041 ggcagcacgg cggcgcgcag agctggaagc ttctcggagc ctgtgggcgc tgctgcagga
     2101 gctggaggag gcggagagtt gggcgcggga caaggagcgc ctcctagagg ccacgagcgg
     2161 cagtggtggc gcggcgggca cggcgggcgg cgcgcacgac ctgtccagca ccgctcgcct
     2221 gctggcgcag cataagatcc tgcagggcga gctgggcggc cggcgggcgc tgctgcagca
     2281 ggcgctgcga cgcggggagg agttggctgc ggcgggcggc tccgtgggcc cgggcgccga
     2341 gcctttgcat ttggctggcc tggcggagcg cgcggctagc gctcggcgcc gctggcagcg
     2401 gctggaagag gcggctgcgc gccgggaacg gcggctgcag gaggcgcggg cgctgcacca
     2461 atttggcgcc gacctggacg ggctgctgga ctggcttcgt gacgcgtatc gtctagcagc
     2521 cgctggagat tttggtcacg acgaagcgtc cagccgtcgc ctggcgcgcc agcaccgcgc
     2581 tctcaccggg gaggtggaag cgcaccgtgg ccctgtgggt ggcctgaggc gccagctggc
     2641 gacactggga ggagccagtg gcgcagggcc gctggtggtg gctctgcagg tgcgcgtggt
     2701 ggaagccgaa cagttgttcg ccgaggtgac cgaggtggcc gcgctgcgac gacagtggct
     2761 tcgggacgcg ctcgccgtgt accgcatgtt cggcgaggta catgcctgcg agttgtggat
     2821 cggggagaaa gagcagtggc tgctggccat gcgtgtgccc gattccctcg acgacgttga
     2881 ggtggtgcag catcgatttg aaagtcttga ccaggaaatg aacagcctga tgggccgagt
     2941 cctggatgtg aaccaaacag ttcaggagtt ggtagaagga ggtcatccca gctccgatga
     3001 ggtgcgctcc tgtcaggacc atctcaacag caggtggaac cgcatcgtgg agttggtgga
     3061 acagcgcaaa gaggagatga gcgcggtgct gctggtggag aaccacgtgc tggaggtggc
     3121 cgaggtgcgc gcccaagtgc gcgagaagcg acgggcagtg gagagcgcgc cccgggcggg
     3181 gggagccctg caatggcgcc tgagtggcct ggaggcggct ctacaggcgc tagagccgcg
     3241 acaggcggcg ctgctggagg aggcggcgct gctggccgaa cgcttccccg cacaggcgac
     3301 gcgcctgcac cagggcgccg aggagctagg agccgagtgg ggcgcactgg ccggagcggc
     3361 tcaggcctgc ggcgaggcgg ttgcggcggc aggtcgcctg cagcgtttcc tgcgcgattt
     3421 ggatactttc ctggactggc tggtgcgggc gcaggaggca gcaggcgccg tcgaggggcc
     3481 gctgcctcgc agcctggagg aggcggatgg gctgctggcg cgccacgccg cgctcaagga
     3541 ggaggtggat cagcgtgagg aggactacgc gcgcatcgtg gctgccagtg aagcgctgct
     3601 agcgtccgag ggcgcggagc tgggccccgg tctggcgcta gacgagtggc tgccgcacct
     3661 cgaggttggc tggcacaaac tgctcggctt gtgggaggag cgcagggagg cgctggtcca
     3721 agcgcacgtc tatcaactct tcctgagaga cctgtgccag gcgcttgcgg tgctgcgcaa
     3781 ccaggaggtg gcgctgtctg gcgctgaact cccttgcaca gtggagtcgg tagaagaggc
     3841 gatgaaaagg caccgggatt ttctcaccac gatggagctg aaccagcaaa agatgcaggt
     3901 ggcggttcaa gctgcagaga gcctgctgcg gcaaggcaac gcctacgggg agcaggcgca
     3961 ggaggcagtg gctcggctgc tagagaagag ccaagaaaat cagctgaggg cccagcagtg
     4021 gatgcaaaaa ctactggacc agcttgtgct gcagcacttt cttagagatt gtcatgagct
     4081 ggatggctgg atccatgaga agatgctgat ggctcgtgat ggcacccggg aagacagcca
     4141 caagctgcac aagagatggc tccggcacca ggcgttcatg gctgagctgg ctcagaataa
     4201 ggagtggctg gagaagatag aaagggaggg ccagcaactg atgcaggaga aaccggagct
     4261 ggcagcctct gtgcgcaaga aattaggtga gatccggcag tgctgggcgg agctggagag
     4321 caccacacag gccaaggcgc gccagctctt cgaggccagc aaggcggacc agctagtgca
     4381 gagcttcgcg gagctggaca agcggctgct tcacatggaa agccagctgc aagacgtaga
     4441 ccccggtggg gacctggcca ctgtcaacag ccagctcaag aagttacagt ccatggagtc
     4501 tcaggtggag gagtggtgcc gagaggtggg cgagcttcag gcgcagacgg cggcgcttcc
     4561 gctggagcag gcgagcaagg agttggtggg ggagcggcag agtgcggtgg gcgagcgcct
     4621 ggtgcgcctg ctggagcctt tgcaggagcg acgccgcttg ttgctggcat ccaaggagct
     4681 gcatcaggtg gcccacgacc tggacgatga actggcttgg gttcaagagc ggctgccact
     4741 ggccatgcag acagagcgag gcactggctt gcaggctgtc cagcagcaca tcaaaaagaa
     4801 ccagggcctg cggcgggaga tccaggctca cgggccgcgc ttggaggagg tgctggagcg
     4861 cgcgggcgtg ctggcctcgc tgcgcagccc agaggccgaa gcggtgcgcc gtggccaaga
     4921 gcagctgcag agcgcctgga ccgggctgcg ggaagcggcc gaacggcggc agcagacgct
     4981 ggacgccgcc ttccaggtgg aacagtacta ctttgacgtg gctgaggtgg aggcatggct
     5041 gggcgagcag gagctgctca tgatgagtga ggacaagggc aaggatgaac agagcaccct
     5101 gcagctgctc aagaagcatt tacaactgga acagggcgtg gagaactatg aggaaagcat
     5161 cgcccagctg tcacgccagt gccgggcgct actggaaatg ggacacccag acagtgagca
     5221 aatcagccgc cgacagtccc aagtggatcg cctctatgtg gcgctcaagg agctgggcga
     5281 ggaacgcagg gtgagcctgg aacagcagta ctggctctac cagctcagcc gccaggtgga
     5341 tgagctggaa cactggatag ccgagaagga ggtggtagct ggctccccag agctgggcca
     5401 ggacttcgaa cacgtgtcgg tgctacagga gaaattctca gagtttgcca gtgagacagg
     5461 aaccgcaggg cgggagcggc tggcggcggt caaccagatg gtggacgagc tgattgagtg
     5521 tggtcacaca gcagcggcca ccatggctga gtggaaggac gggctgaacg aggcctgggc
     5581 tgagctgctg gaactcatgg gcactcgggc ccagctgctc gctgcctctc gggagctgca
     5641 taagttcttc agcgatgccc gggagcttca agggcagatt gaggagaagc ggaggaggct
     5701 gccccgcctg acggcaccac ctgaacccag acccagtgcc agctccatgc agcggaccct
     5761 gcgagccttt gagcacgacc tgcagctcct cgtgtcccag gtacggcagc tgcaggaagg
     5821 ggcggcccag ctgcggacgg tgtacgctgg cgagcacgcc gaggccatcg ccagccgcga
     5881 gcaggaggtg ttgcagggct ggaaggagct gctagcagcc tgtgaagatg ctcgtctgca
     5941 cgtcagctcc acggccgacg ccctgcgctt ccacagccag gcccgcgacc tgctctcctg
     6001 gatggacggc atcgcgggcc agatcggggc agctgacaaa cccagggatg tgtcatcggt
     6061 ggaggtgctt atgaactacc accagggcct gaagacagag ttggaggcgc gcgtgcctga
     6121 gctggcaacc tgccaggagc tggggcggtc tctattactc aacaaaagcg ccatggctga
     6181 tgagatccag gcccagctgg acaagctggg aagccggaag gaggaggtgt cggagaagtg
     6241 ggaccgtcac tgggagtggc tgcagcagat gctggaggta caccagtttg ctcaggaggc
     6301 cgtggtggct gacgcctggc tgacagctca ggaaccgctc ctgcagagcc gggagctggg
     6361 cagcagtgtg gatgaggtgg agcagcttat ccggcgacac gaggccttcc gaaaagcagc
     6421 tgcggcctgg gaggagaggt tcagctctct gcggcgcttg accacgatcg agaaactcaa
     6481 ggcagaacag agtaagcagc cacccacccc cttactgggg cgcaagttct ttggggaccc
     6541 cacagagctg gcggccaagg cagcgccttt gctgcgtcca gggggctatg acaggggcct
     6601 ggagcctttg gcccgcaggg cctcggacac gctgtcagcg gaggtgcgga cccgggttgg
     6661 gtatgtgcgc caggagctca agcccgagcg tctccagccg cgcattgacc ggctgccaga
     6721 gacctcgggg aaggtagaac ccgcggcccc gacagccgca gcgttggaca cgacggacac
     6781 ccctgggact ccggcggcga cggagttggt ccggccccgg tcggagcgcc aggagttagc
     6841 ggatcgtgcg gaggagctgc cacggagacg aagatcagaa cgccaggagt ccgtggatca
     6901 accagaggag acagcgcgga ggcggcggcc tgagcgccag gagtccgcgg accacgaggg
     6961 gccgcacagc cttactctag gtcgctacga gcagatggag agacggcggg agcggaggga
     7021 gcggcggata gaacgacagg agtctagcga acaggagact cccaccaggg gagagctggt
     7081 caaagggaag gccactctgg ctgatattgt ggagcaactt caggagaaag aggcaggccc
     7141 agggatccct gctggggtgc cgtcgctgcc tcagcctcgc gagcttcccc ctggtcgcct
     7201 gcccaacggg cttgagccgc ccgagcggac acctcggccc gatcggccgc gggcacggga
     7261 ccggcccaag ccgcgccggc ggccacggcc tcgagagggt ggtgagggcg ggggaagccg
     7321 gcgctcgcgc tccgcccctg cccagggagg ctccgccccc gcgccgccac ctccgcccac
     7381 tcacacagtg cagcacgagg gcttcctgct gcgcaagcgc gagctcgacg ctaaccgcaa
     7441 gtcgtccaac cggtcatggg tgagcctgta ctgtgtgctc agcaaggggg agctgggctt
     7501 ctacaaggac tccaaaggcc cagcatcagg gggcacgcat ggtggggaac cactgctcag
     7561 cctgcacaaa gccaccagcg aggtggctag tgactacaag aaaaagaagc atgtcttcaa
     7621 gctccagacc caggatggca gtgagttctt gctccaggct aaagatgagg aggagatgaa
     7681 cggctggctg gaggctgtgg ctaactccgt ggcagaacac gccgagatcg cccgatgggg
     7741 tcagacacta cctaccacat catccacaga tgagggcaac cccaagcgtg agggcgggga
     7801 gcgtagggcc agcgggcgcc ggaagtgatt ccaggtaccc tttcccccac cttggagtct
     7861 gacaagtctc cttgcccctc ctctctgcac tgtggacaca aagacaactt ctcatccgca
     7921 agggccggaa tgactgcgga tgtgccactg tgggcctgaa atagaaggga cttctgcact
     7981 ccaagaggtg gtggtagggg attgctgtcc ctatagccat atctcggtcc cttcccgctc
     8041 gccaacccca ccccaggtgc tggggctcca ttctttttat gcaataactg agcttggtgg
     8101 ggccgggcag ggagccagtt gagccaagct ccctgcccaa tcgtcagatc ctgccccaag
     8161 aagctggagt ggcgagggat ctaggaggtg gtgatttgtt ggcctcctct ccgccctaag
     8221 gatggacaac gggggcgctg gtgaggtccc ctggaccatc cagggtgcta gggggtgggg
     8281 aggggacaca ctccctccct gcctttacct cacttccaat gctgccttga tctgtctggg
     8341 aagagggagg acaggggccc ttagcccctg tactccgcca tctcagagcc atgcggttaa
     8401 ttcctgactt agtttatttt tgcaaaaacg tagacccccc ctccttcccc ccgccccgca
     8461 tccgcgaagg cttttaatag gaggggagtc aaagttcaaa actgtcttcc tctctcctcc
     8521 ccgattctag ctgtaaatgc cacttcccga gggggagggg gtgggaaagc cctccccctg
     8581 catgcttctg gctggagcac ctccctggga cggagggaat cagggttaca ggcaaccccc
     8641 gtggctgctt ggagacgctg ccaaggcagg gggtgcgggg cggtgtggca ggcgcacact
     8701 gtatgtacct ataataaacc ctttggcttt gacagtc
//
