LOCUS       NM_175836               8265 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript
            variant 1, mRNA.
ACCESSION   NM_175836
VERSION     NM_175836.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Hagan,M.L., Tuladhar,A., Yu,K., Alhamad,D.W., Bensreti,H., Dorn,J.,
            Piedra,V.M., Cantu,N., Stokes,E.G., Blumenthal,D., Roberts,R.L.,
            Balayan,V., Bass,S.M., Dickerson,T., Cartelle,A.L.,
            Montesinos-Cartagena,M., Awad,M.E., Castro,A.A., Garland,T. Jr.,
            Cooley,M.A., Johnson,M., Hamrick,M.W., McNeil,P.L. and
            McGee-Lawrence,M.E.
  TITLE     Osteocyte Sptbn1 Deficiency Alters Cell Survival and
            Mechanotransduction Following Formation of Plasma Membrane
            Disruptions (PMD) from Mechanical Loading
  JOURNAL   Calcif Tissue Int 115 (5), 725-743 (2024)
   PUBMED   39276238
  REMARK    GeneRIF: Osteocyte Sptbn1 Deficiency Alters Cell Survival and
            Mechanotransduction Following Formation of Plasma Membrane
            Disruptions (PMD) from Mechanical Loading.
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   Martinez-Mayer,J., Brinkmeier,M.L., O'Connell,S.P., Ukagwu,A.,
            Marti,M.A., Miras,M., Forclaz,M.V., Benzrihen,M.G., Cheung,L.Y.M.,
            Camper,S.A., Ellsworth,B.S., Raetzman,L.T., Perez-Millan,M.I. and
            Davis,S.W.
  TITLE     Knockout mice with pituitary malformations help identify human
            cases of hypopituitarism
  JOURNAL   Genome Med 16 (1), 75 (2024)
   PUBMED   38822427
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8265)
  AUTHORS   Sert,O., Ding,X., Zhang,C., Mi,R., Hoke,A. and Rasband,M.N.
  TITLE     Postsynaptic beta1 spectrin maintains Na+ channels at the
            neuromuscular junction
  JOURNAL   J Physiol 602 (6), 1127-1145 (2024)
   PUBMED   38441922
  REMARK    GeneRIF: Postsynaptic beta1 spectrin maintains Na[+] channels at
            the neuromuscular junction.
REFERENCE   4  (bases 1 to 8265)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 8265)
  AUTHORS   Guo,P., Lim,R.C., Rajawasam,K., Trinh,T., Sun,H. and Zhang,H.
  TITLE     A methylation-phosphorylation switch controls EZH2 stability and
            hematopoiesis
  JOURNAL   Elife 13, e86168 (2024)
   PUBMED   38346162
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8265)
  AUTHORS   Moskow,J.J., Bullrich,F., Huebner,K., Daar,I.O. and Buchberg,A.M.
  TITLE     Meis1, a PBX1-related homeobox gene involved in myeloid leukemia in
            BXH-2 mice
  JOURNAL   Mol Cell Biol 15 (10), 5434-5443 (1995)
   PUBMED   7565694
REFERENCE   7  (bases 1 to 8265)
  AUTHORS   Zimmer,W.E., Ma,Y., Zagon,I.S. and Goodman,S.R.
  TITLE     Developmental expression of brain beta-spectrin isoform messenger
            RNAs
  JOURNAL   Brain Res 594 (1), 75-83 (1992)
   PUBMED   1467942
REFERENCE   8  (bases 1 to 8265)
  AUTHORS   Bloom,M.L., Lee,B.K., Birkenmeier,C.S., Ma,Y., Zimmer,W.E.,
            Goodman,S.R., Eicher,E.M. and Barker,J.E.
  TITLE     Brain beta spectrin isoform 235 (Spnb-2) maps to mouse chromosome
            11
  JOURNAL   Mamm Genome 3 (5), 293-295 (1992)
   PUBMED   1638088
REFERENCE   9  (bases 1 to 8265)
  AUTHORS   Zimmer,W.E., Ma,Y.P. and Goodman,S.R.
  TITLE     Identification of a mouse brain beta-spectrin cDNA and distribution
            of its mRNA in adult tissues
  JOURNAL   Brain Res Bull 27 (2), 187-193 (1991)
   PUBMED   1742606
REFERENCE   10 (bases 1 to 8265)
  AUTHORS   Birkenmeier,C.S., Bodine,D.M., Repasky,E.A., Helfman,D.M.,
            Hughes,S.H. and Barker,J.E.
  TITLE     Remarkable homology among the internal repeats of erythroid and
            nonerythroid spectrin
  JOURNAL   Proc Natl Acad Sci U S A 82 (17), 5671-5675 (1985)
   PUBMED   3862089
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL672225.15, AK169544.1, AK157727.1, CK627078.1, CF539329.1,
            AF017112.1, AK051630.1 and BC024833.1.
            
            On Nov 13, 2006 this sequence version replaced NM_175836.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and it encodes the longer protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849382,
                              SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-194               AL672225.15        77831-78024         c
            195-693             AK169544.1         535-1033
            694-2010            AK157727.1         1024-2340
            2011-2542           CK627078.1         102-633
            2543-3203           CF539329.1         55-715
            3204-6463           AF017112.1         3451-6710
            6464-7912           AK051630.1         822-2270
            7913-8265           BC024833.1         1175-1527
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 17.44 cM"
     gene            1..8265
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /note="spectrin beta, non-erythrocytic 1"
                     /db_xref="GeneID:20742"
                     /db_xref="MGI:MGI:98388"
     exon            1..194
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..7138
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     spectrin beta chain, brain 1; beta-II spectrin; fodrin
                     beta chain; embryonic liver fodrin; spectrin,
                     non-erythroid beta chain 1; beta-spectrin 2,
                     non-erythrocytic; brain spectrin; beta fodrin; spectrin G;
                     spectrin beta chain, non-erythrocytic 1; spectrin beta 2"
                     /codon_start=1
                     /product="spectrin beta chain, non-erythrocytic 1 isoform
                     1"
                     /protein_id="NP_787030.2"
                     /db_xref="CCDS:CCDS36123.1"
                     /db_xref="GeneID:20742"
                     /db_xref="MGI:MGI:98388"
                     /translation="MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSR
                     IKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPK
                     PTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQ
                     DISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPD
                     LIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHY
                     FSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSL
                     VGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDI
                     NKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNF
                     GFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIR
                     LWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDL
                     LQKHALVEADIAIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELC
                     QLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRA
                     FEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRL
                     EEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEITNYRP
                     TIDTLHEQASALPQAHAESPDVKGRLAGIEERCKEMAELTRLRKQALQDTLALYKMFS
                     EADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQ
                     LMHNGHPSEKEIRAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIR
                     EKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQA
                     QAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASE
                     DMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQA
                     LDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGA
                     EAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRE
                     AASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFM
                     AELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFD
                     ANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEI
                     EELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLSERKHNLLASKEIHQFNRDV
                     EDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNII
                     TDSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHKAQQYYFDAAEAEAWMSE
                     QELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESER
                     ISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHEL
                     GQDYEHVTMLQERFREFARDTGNIGQERVDTVNNMADELINSGHSDAATIAEWKDGLN
                     EAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETL
                     QRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDAC
                     EGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKA
                     EIDARNDSFTACIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLI
                     LEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFS
                     ALERLTTLELLEVRRQQEEEERKRRPPSPDPNTKVSEEAESQQWDTSKGDQVSQNGLP
                     AEQGSPRMAGTMETSEMVNGAAEQRTSSKESSPVPSPTLDRKAKSALPAQSAATLPAR
                     TLETPAAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKSAASGIPY
                     HSEVPVSLKEAICEVALDYKKKKHVFKLRLSDGNEYLFQAKDDEEMNTWIQAISSAIS
                     SDKHDTSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK"
     exon            195..346
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            347..520
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            521..612
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            613..693
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            694..809
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            810..922
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1110
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1228
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1387
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1690
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1844
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..2715
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2716..2853
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2854..3610
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3611..3813
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3814..3904
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3905..4051
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4052..4315
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4316..4540
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4541..4630
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4631..4912
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4913..5043
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            5044..5248
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            5249..5623
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            5624..5868
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            5869..6007
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6008..6092
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6093..6289
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6290..6399
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6400..6463
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6464..6702
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6703..6876
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6877..6919
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     exon            6920..8239
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8220..8225
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /note="hexamer: ATTAAA"
     polyA_site      8239
                     /gene="Sptbn1"
                     /gene_synonym="9930031C03Rik; elf1; elf3; mKIAA4049;
                     Spnb-2; Spnb2; SPTB2"
                     /note="major polyA site"
ORIGIN      
        1 aactctgaaa agtaactgat gtggacaagc agctgagaca gtcaagatga cgaccacggt
       61 agccacagac tatgacaaca ttgagatcca gcagcagtac agtgatgtga acaaccgctg
      121 ggatgtggat gactgggaca atgagaacag ctctgcaagg ctctttgagc gctcccgcat
      181 caaggccctg gcagatgagc gtgaagctgt acagaagaag accttcacca agtgggtcaa
      241 ttcccacctt gcgagagtgt cctgccgaat cacagacctg tacacggacc ttcgagatgg
      301 acggatgctc atcaagctac tggaggtcct ctctggagag aggctgccta aacccactaa
      361 gggacggatg cggatccact gtctggagaa tgtcgacaag gctcttcaat tcctgaaaga
      421 gcagagagtc catcttgaga acatgggctc ccatgacatt gtggatggaa accaccggct
      481 gaccctcggc ctcatctgga caattattct gcgcttccag atccaggata ttagtgtgga
      541 gactgaagat aacaaagaga aaaagtctgc taaggatgca ttgctgctgt ggtgccagat
      601 gaagacagct gggtacccca atgtcaacat tcacaatttc accactagct ggagggatgg
      661 catggccttc aatgcactga tacataaaca tcggcctgac ctgatagatt ttgataaact
      721 gaagaaatct aatgcacact acaatctgca gaatgcattt aacctggcag agcagcacct
      781 tggcctcact aaactgttag accctgaaga tatcagtgtg gaccaccctg atgagaagtc
      841 tatcatcaca tacgtggtga cttactacca ctacttctcc aagatgaagg ccttggctgt
      901 cgaaggaaag cgcattggaa aggtgcttga taatgctata gaaacagaga aaatgattga
      961 gaagtacgag tcacttgctt ctgaccttct ggagtggatt gaacaaacca tcatcatcct
     1021 aaacaaccgc aaatttgcta attcactggt tggggtccag cagcagctcc aggcattcaa
     1081 cacgtaccgc acagtggaga aaccacctaa gtttactgag aaggggaatt tggaggtgct
     1141 ccttttcaca attcagagca agatgcgagc gaataatcag aaggtctaca tgccccgcga
     1201 ggggaagctc atctctgaca tcaacaaggc ctgggaaaga ctggaaaaag cagaacatga
     1261 gagagaactg gctctgcgga atgagctcat acggcaggaa aaactggaac aactcgcccg
     1321 aagatttgat cgcaaggcag ctatgaggga gacatggctg agtgaaaacc agcgtcttgt
     1381 gtctcaggac aactttggat ttgaccttcc cgctgttgag gctgctacca aaaaacacga
     1441 ggccattgag acagacatcg ctgcatatga agaacgagtt caggccgtgg tggctgtggc
     1501 cagggaactt gaagccgaga actaccatga catcaagcgc atcacagcga ggaaggacaa
     1561 tgtcatccgg ctctgggaat acttgctgga actgctcagg gccaggaggc agcgtcttga
     1621 gatgaacctg ggattgcaaa agatattcca ggaaatgctt tatattatgg actggatgga
     1681 tgaaatgaag gtgctattgc tgtctcaaga ctatggcaaa cacttacttg gtgttgaaga
     1741 cctgttacag aagcatgccc tggttgaagc agacattgca atccaagcag agcgtgtaag
     1801 aggtgtgaat gcctctgccc agaagtttgc aacagatggg gaaggctaca agccatgtga
     1861 cccccaggta attcgagacc gtgttgccca catggagttc tgctatcaag agctttgtca
     1921 gctggctgcc gagcgtaggg ctcgcctgga agagtcccgt cgcctctgga agttcttctg
     1981 ggagatggca gaagaggaag gctggatacg agagaaggag aagatcctgt cctctgatga
     2041 ttacgggaaa gacttgacca gtgtcatgcg cctgctgagc aagcaccggg catttgagga
     2101 tgagatgagt ggccgtagtg gccattttga gcaggccatt aaagaaggtg aagacatgat
     2161 tgcagaggaa cactttggat cggaaaagat ccgtgagaga atcatttata tccgggagca
     2221 gtgggccaac ctggaacagc tctcagccat taggaagaag cgcctagagg aagcctcatt
     2281 actgcaccag ttccaggctg atgctgatga tattgatgct tggatgttag atatactcaa
     2341 gattgtctcc agcaatgatg tgggccatga tgagtactcc acgcagtctc tggtcaagaa
     2401 gcataaagat gtagcagaag agatcaccaa ctacaggccc actattgaca cactgcatga
     2461 gcaagccagt gcccttccac aagcacatgc agagtctcca gatgtgaagg gccggctggc
     2521 aggaattgag gagcgctgca aggagatggc agagttaaca cggctaagga agcaggctct
     2581 gcaggacacc ctggccctgt acaagatgtt cagtgaggct gatgcctgtg agctctggat
     2641 tgacgagaag gagcagtggc tcaacaacat gcagatccca gagaagctgg aggacctgga
     2701 agtcatccag cacagatttg agagcctaga accagaaatg aacaaccagg cttcccgggt
     2761 tgctgtggtg aaccagattg cacggcagct gatgcacaat ggccacccca gtgaaaagga
     2821 aatcagagct cagcaagaca aactcaacac gaggtggagt cagttcagag aactggtgga
     2881 caggaaaaag gatgctcttc tgtctgccct gagcatccag aactaccacc tcgagtgcaa
     2941 tgaaaccaaa tcctggatcc gggagaagac caaggtcatc gagtctaccc aagaccttgg
     3001 caatgacctg gcaggtgtca tggccctgca gcgcaagctg actggcatgg aacgagactt
     3061 ggtagccatt gaggcgaagc tgagtgacct gcagaaagaa gctgagaagc tggagtccga
     3121 gcaccctgac caggctcaag ctatcctgtc tcggctggcc gagatcagtg atgtgtggga
     3181 ggaaatgaag acaaccctga agaaccgaga ggcctccctg ggagaggcca gcaagctgca
     3241 gcagtttctg cgggacttgg acgacttcca gtcttggctc tccaggaccc agactgctat
     3301 cgcctcagag gacatgccca ataccctcac tgaggcagag aagcttctca cacagcacga
     3361 gaatatcaaa aatgagatcg acaattatga ggaagactac cagaagatgc gggacatggg
     3421 cgagatggtc acccaggggc agactgatgc ccagtatatg tttctgcggc agcggctgca
     3481 ggccttagac actggctgga atgagctcca caaaatgtgg gagaacaggc aaaacctcct
     3541 ctcccagtcc catgcctacc agcagttcct tagggacacc aaacaagctg aagcttttct
     3601 taataaccag gagtatgttt tggctcatac tgaaatgccc accaccctgg aaggagctga
     3661 agcagccatt aaaaagcagg aggacttcat gaccaccatg gatgccaacg aggagaagat
     3721 caatgctgtt gtggagactg gccgaagact ggtgagcgat gggaacatca actccgaccg
     3781 catccaggag aaggtggact ctattgacga cagacacagg aagaatcgag aagcagccag
     3841 tgaacttctg atgaggttaa aggacaaccg tgatctacag aagttcctgc aagattgtca
     3901 agagctgtcc ctctggatca atgaaaagat gcttacagct caagacatgt cttatgatga
     3961 agccagaaat ctgcacagta aatggttaaa gcatcaagca tttatggcgg aacttgcatc
     4021 caacaaagaa tggcttgaca aaattgagaa ggaaggaatg cagcttattt cagaaaagcc
     4081 agaaacagaa gctgtggtaa aggaaaaact cactggttta cataaaatgt gggaagtcct
     4141 tgaatccaca acccagacca aggcccagcg gctctttgat gcaaataagg ctgagctttt
     4201 cacacaaagc tgcgcagatc ttgacaaatg gctacatggc ctggagagcc agattcaatc
     4261 tgacgactat ggcaaagacc ttaccagtgt caatattctt ctgaaaaagc aacagatgct
     4321 ggagaatcag atggaagttc ggaagaaaga gatcgaggaa ctgcagagcc aagcccaggc
     4381 gctgagtcag gaggggaaga gcacagatga ggtggacagc aaacgcctta ctgtgcagac
     4441 caagttcatg gagcttctgg agcccttgag tgagaggaag cataacctgt tagcttccaa
     4501 ggagatccat cagttcaaca gggatgtgga ggacgaaatc ctatgggttg gcgagaggat
     4561 gcctttggca acttccacag atcatggcca taaccttcaa actgtgcagc tgttaataaa
     4621 gaaaaaccag accctccaga aagaaatcca gggacaccag cctcgtattg atgacatctt
     4681 tgagaggagt caaaacatca tcacagatag cagcagcctc aatgccgagg ctatcaggca
     4741 gaggctcgct gacctgaagc agctgtgggg gctcctcatt gaggaaactg agaaacgcca
     4801 tagacggctg gaggaggcac acaaggcgca gcagtactac tttgatgcag ctgaagccga
     4861 ggcatggatg agtgaacagg agttgtacat gatgtctgag gaaaaggcca aggatgagca
     4921 gagtgctgtc tctatgttga aaaagcacca gattttagag caagctgttg aggactatgc
     4981 agagacagta caccagctct ccaagactag ccgggcgctg gtggctgaca gccatcccga
     5041 aagtgagcgt attagcatgc ggcagtcaaa ggtcgacaag ctgtatgctg gcctgaagga
     5101 ccttgctgag gagaggagag gaaaacttga tgagaggcac aggctgttcc agctcaacag
     5161 agaggtggat gacctggaac agtggatcgc tgagagggaa gtggtcgcag gctcccatga
     5221 gttgggacag gactatgagc atgtcacgat gttacaagaa cggttccgag aatttgctcg
     5281 agacacagga aacattgggc aggagcgtgt ggatacagtt aataacatgg cagatgaact
     5341 catcaactct ggacattcag atgctgccac cattgctgag tggaaagatg gtctcaatga
     5401 agcctgggct gacctcctgg agctcattga cacaagaaca cagattcttg ctgcctcata
     5461 tgaacttcat aagttttacc atgatgccaa ggagatcttt ggccgaatcc aggacaaaca
     5521 caagaaactc cctgaggagc ttggaagaga tcaaaacact gtggaaactt tacagagaat
     5581 gcacaccacc tttgagcacg acatccaagc tctgggcact caggtgaggc agctgcagga
     5641 ggatgcagct cgcctccagg cagcctatgc aggggacaag gctgatgaca tccagaagcg
     5701 tgagaatgag gtcctggaag cctggaagtc cctgctggat gcttgtgagg gtcgcagggt
     5761 gcggctggta gacacaggag acaagttccg cttcttcagc atggtgcgtg acctcatgct
     5821 ctggatggaa gatgtcatcc ggcagatcga ggcccaggag aaaccacggg atgtgtcatc
     5881 tgttgaactg ttaatgaata atcatcaagg tatcaaagct gaaattgatg ctcgtaatga
     5941 cagctttaca gcctgcattg agcttgggaa atccctgctg gcacggaaac actatgcttc
     6001 tgaggagatc aaggaaaagt tactgcagct gacagagaaa agaaaagaaa tgattgacaa
     6061 gtgggaagac cggtgggagt ggttaagact gattttggag gtccatcagt tctcaaggga
     6121 tgccagtgtg gcagaggctt ggctgcttgg acaggaacca tacctatcca gccgtgaaat
     6181 tggccagagt gtagacgaag tggagaagct tattaagcgc catgaggcgt ttgaaaagtc
     6241 tgcagcgacc tgggatgaga gattctctgc tctggaaagg ctgacaacgt tggagctact
     6301 ggaagtgcgc agacagcaag aggaagaaga aagaaagagg cggccacctt ctccggaccc
     6361 aaacacgaag gtttcagagg aggctgagtc ccagcaatgg gatacttcaa aaggagacca
     6421 agtttcccag aatggtttgc cggctgagca gggatctcca cggatggcag gaaccatgga
     6481 aacgagtgaa atggtcaacg gtgctgctga gcagaggaca agctccaaag agtccagtcc
     6541 tgttccctct cccaccttgg accgaaaggc caaatctgca cttccagccc agagtgctgc
     6601 caccctgcca gccaggaccc tggagacacc cgctgcccag atggaaggct tcctcaatcg
     6661 gaagcatgag tgggaggccc acaataagaa agcctcgagc aggtcctggc acaatgtata
     6721 ttgtgtcata aataaccaag aaatgggctt ctataaagat gccaagagtg ctgcttctgg
     6781 catcccctac cacagtgagg tccctgtgag tttgaaagag gccatctgcg aagtggccct
     6841 tgattacaaa aagaagaagc acgtgttcaa gctaagacta agtgatggaa acgagtacct
     6901 cttccaagcc aaagatgatg aggaaatgaa cacatggatc caggctatct cctctgccat
     6961 ctcctctgac aaacacgaca catctgccag cacccagagt acgccagcat ccagtcgggc
     7021 gcagacctta cccaccagcg tcgtcaccat caccagcgag tccagtcctg gcaagaggga
     7081 gaaggataaa gagaaagaca aagagaagag gttcagcctt ttcggcaaga agaagtgaac
     7141 ttccctgttg tcccgccctt ctcttacctt tagtaagagt ccagcatgca agttccgacc
     7201 aacacattac tctgtgccta atgttcctcc atgtagttga ttttttttct tttctttttt
     7261 ttttattttt gaaataaatt ttttaattta tagagtattt ctgaggggtg ggggaaccat
     7321 acctaaacac tttatctcca agtttaaaaa gtttgaggta cagagggaag gccagatttt
     7381 tttaatgaaa ttatatagat tagatctcag tatttaaact gttcctcaat tttgtgaggc
     7441 tgtgttggaa ataacccgcc tctgatgctg ttggtatgca aggcagtggt gcttacacaa
     7501 tatttcctgt gctctccaga gacgatggac cgatttcctg acactactct cccttcactt
     7561 ccgtggttac cctgagtctt gacttacaaa tgcccacgat gggtgtagcc tttattaaac
     7621 agatcatgta ttctgatctc ttgctgcagc cacagtgcag ctccatgtaa acctacaggc
     7681 caaaccattt gtatccggca tcacttacta acacacaaca tgtggctttt ctgcatcaat
     7741 tgctgtgaca gttcagaatg ttggtataca agaaagaata gaaaattgat aaggttttaa
     7801 ataatctgta atttcagtct ctttttttct gaaatacatt atattgtatg tttgagataa
     7861 ttctagtaca aagtataata aactagatgt ataataaacc ctttaaatca ttgctaagtg
     7921 tataagtgga actgaagcat ttactggaca aagtaatgtt actctaatgg ttacttgctt
     7981 gtgcgtggcc acactgttat aatttgcttc attaccctgc tatttgatac atagtgtgca
     8041 tttctctgtc actgtaacta ttgtaataac aaattttcat cttactgcac aatcaaaatg
     8101 gcatctatag taatgacctc cagagtctga gcttggacag agagtgggcg ctcaggcctg
     8161 gtgctccatc atacgacctg tacctctcaa cttttgccct gttaaatata tgctatgtca
     8221 ttaaatgctt ttaaatctaa aaaaaaaaaa aaaaaaaaaa aaaaa
//
