LOCUS       NM_001177668            7979 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1),
            transcript variant 3, mRNA.
ACCESSION   NM_001177668
VERSION     NM_001177668.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7979)
  AUTHORS   Wang,J., Jia,C., Gao,Q., Zhang,J. and Gu,X.
  TITLE     iASPP regulates neurite development by interacting with Spectrin
            proteins
  JOURNAL   Front Mol Neurosci 16, 1154770 (2023)
   PUBMED   37284462
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7979)
  AUTHORS   Yan,L., Yin,H., Mi,Y., Wu,Y. and Zheng,Y.
  TITLE     Deficiency of Wdr60 and Wdr34 cause distinct neural tube
            malformation phenotypes in early embryos
  JOURNAL   Front Cell Dev Biol 11, 1084245 (2023)
   PUBMED   37228654
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7979)
  AUTHORS   Rudolf,M.A., Andreeva,A., Kim,C.E., DeNovio,A.C., Koshar,A.N.,
            Baker,W., Cartagena-Rivera,A.X. and Corwin,J.T.
  TITLE     Stiffening of Circumferential F-Actin Bands Correlates With
            Regenerative Failure and May Act as a Biomechanical Brake in the
            Mammalian Inner Ear
  JOURNAL   Front Cell Neurosci 16, 859882 (2022)
   PUBMED   35602553
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7979)
  AUTHORS   Yao,Q., Wang,H., Chen,H., Li,Z., Jiang,Y., Li,Z., Wang,J., Xing,Y.,
            Liu,F., Yu,D. and Yin,S.
  TITLE     Essential Role of Sptan1 in Cochlear Hair Cell Morphology and
            Function Via Focal Adhesion Signaling
  JOURNAL   Mol Neurobiol 59 (1), 386-404 (2022)
   PUBMED   34708331
REFERENCE   6  (bases 1 to 7979)
  AUTHORS   Hoopes,C.W., Taketo,M., Ozato,K., Liu,Q., Howard,T.A., Linney,E.
            and Seldin,M.F.
  TITLE     Mapping of the mouse Rxr loci encoding nuclear retinoid X receptors
            RXR alpha, RXR beta, and RXR gamma
  JOURNAL   Genomics 14 (3), 611-617 (1992)
   PUBMED   1358808
REFERENCE   7  (bases 1 to 7979)
  AUTHORS   Zimmer,W.E., Ma,Y., Zagon,I.S. and Goodman,S.R.
  TITLE     Developmental expression of brain beta-spectrin isoform messenger
            RNAs
  JOURNAL   Brain Res 594 (1), 75-83 (1992)
   PUBMED   1467942
REFERENCE   8  (bases 1 to 7979)
  AUTHORS   Pilz,A., Moseley,H., Peters,J. and Abbott,C.
  TITLE     Comparative mapping of mouse chromosome 2 and human chromosome 9q:
            the genes for gelsolin and dopamine beta-hydroxylase map to mouse
            chromosome 2
  JOURNAL   Genomics 12 (4), 715-719 (1992)
   PUBMED   1315305
REFERENCE   9  (bases 1 to 7979)
  AUTHORS   Bloom,M.L., Lee,B.K., Birkenmeier,C.S., Ma,Y., Zimmer,W.E.,
            Goodman,S.R., Eicher,E.M. and Barker,J.E.
  TITLE     Brain beta spectrin isoform 235 (Spnb-2) maps to mouse chromosome
            11
  JOURNAL   Mamm Genome 3 (5), 293-295 (1992)
   PUBMED   1638088
REFERENCE   10 (bases 1 to 7979)
  AUTHORS   Siracusa,L.D., Jenkins,N.A. and Copeland,N.G.
  TITLE     Identification and applications of repetitive probes for gene
            mapping in the mouse
  JOURNAL   Genetics 127 (1), 169-179 (1991)
   PUBMED   1673105
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ054332.1, AK147317.1, BC172095.1 and CX206486.1.
            
            Transcript Variant: This variant (3) lacks an alternate in-frame
            exon in the 3' coding region, compared to variant 4. It encodes
            isoform 3, which is shorter than isoform 4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147317.1, BC172095.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-226               CJ054332.1         2-227
            227-1450            AK147317.1         111-1334
            1451-3379           BC172095.1         1287-3215
            3380-3958           AK147317.1         3265-3843
            3959-7833           BC172095.1         3795-7669
            7834-7979           CX206486.1         141-286
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 20.93 cM"
     gene            1..7979
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /note="spectrin alpha, non-erythrocytic 1"
                     /db_xref="GeneID:20740"
                     /db_xref="MGI:MGI:98386"
     exon            1..226
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            227..466
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     CDS             230..7603
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /note="isoform 3 is encoded by transcript variant 3;
                     spectrin alpha chain, brain; alpha-II spectrin; fodrin
                     alpha chain; spectrin, non-erythroid alpha chain;
                     alpha-spectrin 2, brain; spectrin alpha chain,
                     non-erythrocytic 1; alphaII-spectrin"
                     /codon_start=1
                     /product="spectrin alpha chain, non-erythrocytic 1 isoform
                     3"
                     /protein_id="NP_001171139.1"
                     /db_xref="CCDS:CCDS50559.1"
                     /db_xref="GeneID:20740"
                     /db_xref="MGI:MGI:98386"
                     /translation="MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSY
                     RFQFFQRDAEELEKWIQEKLQVASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKL
                     DETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECED
                     VMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVSQFAAKLIQE
                     QHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETIGWIKEKEQ
                     LMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSASQIQV
                     KREELITNWEQIRTLAAERHARLDDSYRLQRFLADFRDLTSWVTEMKALINADELAND
                     VAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAASHYASDEVREKLSILSEERT
                     ALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKK
                     HEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMHRR
                     AQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSAN
                     QSRIDALEKAGQKLIDVNHYAKEEVAARMNEVISLWKKLLEATELKGIKLREANQQQQ
                     FNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQ
                     ARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEET
                     WIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHF
                     AAEDVKAKLSELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVG
                     STDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELV
                     LALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSA
                     SRENLLEEQGSIALRQGQIDNQYQSLLELGEKRKGMLEKSCKKFMLFREANELQQWIT
                     EKEAALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEE
                     VQAVQQQEVYGAMPRDEADSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEE
                     RSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDL
                     AALGDKVNSLGETAQRLIQSHPESAEDLKEKCTELNQAWTSLGKRADQRKAKLGDSHD
                     LQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAF
                     EQFGQQLLAHGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDHCLELQLFHRDCEQ
                     AENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAV
                     DHYAKGDIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQ
                     TASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGS
                     EDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLAS
                     EDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKEKRDT
                     INGRFQKIKSMATSRRAKLSESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGV
                     QNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGQEEIQQRLAQFVEHWKELKQ
                     LAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAF
                     ETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLD
                     ENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQE
                     GIANITALKDQLLAAKHIQSKAIEARHASLMKRWTQLLANSATRKKKLLEAQSHFRKV
                     EDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADF
                     NQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDK
                     LRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLK
                     KIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVT
                     EEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVD
                     PNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQ
                     ADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN"
     exon            467..592
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            593..733
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            734..880
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1014
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1159
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1314
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1450
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            1451..1552
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1690
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1801
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            1802..1879
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..2035
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2240
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            2241..2422
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            2423..2666
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            2667..2789
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            2790..3007
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            3008..3100
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            3101..3235
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            3236..3384
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            3385..3583
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            3584..3688
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            3689..3712
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            3713..3748
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            3749..3796
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            3797..3888
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            3889..4033
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            4034..4215
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            4216..4315
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            4316..4513
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            4514..4660
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            4661..4764
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            4765..4927
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            4928..4942
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            4943..5074
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            5075..5212
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            5213..5317
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            5318..5526
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            5527..5647
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            5648..5769
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            5770..5902
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            5903..6001
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            6002..6161
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            6162..6238
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            6239..6448
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            6449..6745
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            6746..6858
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            6859..6876
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            6877..6931
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            6932..7128
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            7129..7182
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            7183..7329
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            7330..7477
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     exon            7478..7958
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7933..7938
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /note="hexamer: AATAAA"
     polyA_site      7958
                     /gene="Sptan1"
                     /gene_synonym="2610027H02Rik; Spna-2; Spna2"
                     /note="major polyA site"
ORIGIN      
        1 ggagcagcga gtcccgcctc tcgcgtcgcc aaccggggca cttccctcac gctcgcgctc
       61 ggccgccgcg ggggcgcgcg cggccgctta taaaagccac agtgcgcgcg cgccgccgtc
      121 actatccgct gcggagcgaa cggcgtgagg caggggccgc ggcggcgcat ctttttcctc
      181 agcagccctc agcgcggcga cccacgcccc tcacccccag gagccgaaaa tggatccaag
      241 tggggtcaaa gtgctggaaa cagccgagga catccaggag agacgacagc aagtcctgga
      301 tcggtaccac cgcttcaagg agctctctac cttacggcgt cagaagctgg aggattccta
      361 tcggttccag ttttttcaaa gagatgctga ggagttggag aagtggattc aggagaagct
      421 tcaagttgca tctgatgaga actacaaaga cccaaccaac ttgcagggaa agctgcagaa
      481 gcaccaagcc tttgaagctg aagtacaggc taactcagga gccattgtga agctggacga
      541 gacaggaaac ttgatgattt ctgaagggca ctttgcatct gaaaccatcc ggacacgttt
      601 aatggagctg caccggcagt gggaactgct tttggagaag atgcgggaga aaggaatcaa
      661 actgctgcag gcacagaagc tggtgcagta tttgcgggag tgtgaggatg taatggactg
      721 gatcaatgac aaggaagcaa ttgtgacttc tgaggagctg ggccaggacc tggagcatgt
      781 agaggtgcta cagaagaagt ttgaagagtt tcagactgat ctggctgctc atgaagaaag
      841 agttaatgaa gtgagccagt ttgctgccaa actcatccag gagcagcacc cggaagagga
      901 gctgatcaag accaagcagg atgaggtgaa tgcagcatgg cagcgactga aaggcctggc
      961 tcttcaaagg cagggcaagc tgttcggtgc tgctgaggtc cagcgcttta acagggatgt
     1021 agatgagacc attggttgga ttaaggagaa agagcagtta atggcctctg atgacttcgg
     1081 cagagactta gcaagtgttc aagctctgct tcgaaagcat gagggtctgg agagagatct
     1141 tgctgctcta gaggacaagg tgaaagccct gtgtgctgag gctgaccgcc tgcaacagtc
     1201 acaccctctg agtgccagcc agatccaggt gaagcgagag gagctgatta ccaactggga
     1261 acagatccga actctggcag cagagagaca tgcacggctt gatgactcgt acaggcttca
     1321 gcgctttctt gctgacttcc gtgacctcac aagctgggta actgaaatga aagccctcat
     1381 caatgcagat gaactggcca atgatgtggc tggtgctgaa gccctgctgg acaggcatca
     1441 agagcacaag ggtgagatcg acgctcatga agatagcttt aagtctgcag atgagtctgg
     1501 gcaagctctg cttgcggcta gtcactatgc ctcagatgaa gtgagggaga agctgagcat
     1561 cctctccgag gagagaactg ccctgctgga gctgtgggag cttcggaggc agcagtatga
     1621 gcagtgcatg gacttgcagc tcttctaccg tgacactgag caggtggaca actggatgag
     1681 caagcaggag gcattcctgc taaatgaaga cttgggtgac tccttagaca gtgtggaagc
     1741 tcttttgaag aagcatgagg actttgaaaa atctctcagt gcccaggaag aaaaaatcac
     1801 agcccttgat gaatttgcaa ccaaactcat ccaaaataac cactacgcaa tggaagatgt
     1861 agcgactcga cgagatgctc tcctgagccg ccgcaatgcc cttcatgagc gagccatgca
     1921 tcgccgggca cagctggctg attccttcca tctgcagcag ttcttccgtg attctgatga
     1981 gctcaaaagt tgggtcaacg agaagatgaa aacggccacc gatgaagctt ataaagatcc
     2041 atccaacctg caagggaaag tccaaaagca tcaggctttt gaggctgagc tttcagcaaa
     2101 ccagagccgg attgatgccc ttgagaaagc tgggcagaag ctaatagatg tcaaccacta
     2161 tgccaaggaa gaagtagcag ctcgtatgaa tgaggtcatc agtttgtgga agaaacttct
     2221 agaggccaca gaactgaaag gaataaagct ccgagaagcc aaccagcagc aacaatttaa
     2281 tcgaaatgtt gaagacattg aattgtggct gtatgaagta gaaggtcatt tggcttcaga
     2341 tgattatggt aaagacctca ctaatgtcca gaacctccag aagaagcatg ctctgctaga
     2401 ggcggatgtt gctgctcacc aggatcgaat tgatggcatc acaattcagg cccgccagtt
     2461 ccaagatgct ggccattttg atgccgaaaa tattaagaag aagcaagagg cccttgtggc
     2521 tcgctatgag gctcttaagg aacccatggt ggcccggaaa cagaagctgg cagattctct
     2581 tcgtctgcag cagctcttcc gggatgtgga ggacgaagaa acctggattc gagaaaagga
     2641 gcctattgct gcatccacta acagaggcaa agatcttatt ggagtccaga atctgctaaa
     2701 gaaacaccaa gctttgcaag cagagattgc tgggcatgaa cctcgaatca aagcagtgac
     2761 acaaaaggga aatgccatgg tggaggaagg ccattttgct gcagaggatg tgaaggccaa
     2821 actgagtgag ctcaaccaga agtgggaggc tctgaaagcc aaagcctccc agcggaggca
     2881 ggatctagag gactcgctgc aggcccagca gtactttgcc gatgccaatg aggctgagtc
     2941 ctggatgcgg gagaaggagc ccattgtggg cagtacggac tatgggaagg atgaagactc
     3001 tgctgaggct ctactcaaga agcatgaagc tttgatgtcc gatctcagcg cctatggcag
     3061 cagcatccag gctctgcgag aacaggccca gtcatgccgg caacaagtgg cccccatgga
     3121 tgatgagact gggaaggagc tagtcttggc tctctatgac tatcaagaga agagtcctcg
     3181 tgaggtcacc atgaagaaag gggatatcct caccttgctc aacagcacaa acaaggattg
     3241 gtggaaagtg gaagtgaatg accggcaggg tttcgtgcca gctgcatatg tgaagaagct
     3301 ggaccccgcc cagtcagcct cgagggagaa cctcctagaa gagcagggca gcattgctct
     3361 gcggcagggg caaattgaca accaatatca gtctctgctg gaactgggtg agaagagaaa
     3421 aggcatgttg gagaagagtt gcaagaagtt catgttgttc cgggaagcaa acgagttaca
     3481 gcagtggatc actgagaagg aggctgctct aaccaatgag gaggttggcg ctgacttgga
     3541 gcaggttgag gtgctgcaga agaagtttga tgacttccag aaggatctga aagccaatga
     3601 gtctcggctg aaggacatta acaaagtagc agaagacctg gagtctgaag gtctgatggc
     3661 agaggaagtg caagctgtgc agcagcagga ggtgtatggt gcgatgccca gggatgaagc
     3721 agattccaag acggcctccc catggaagtc tgctcgactg atggtccata cagtggctac
     3781 cttcaactcc atcaaggagc tgaatgagcg ttggcggtcc ctgcaacagc tggctgagga
     3841 acgtagccag ctcttgggca gtgcacacga agtacagagg tttcacagag atgctgatga
     3901 aactaaagaa tggattgaag agaagaatca ggctctgaac acagacaact atggccatga
     3961 tttagctagt gtccaggccc tgcagcgcaa acatgaaggc tttgagaggg accttgcagc
     4021 tcttggtgac aaggtgaatt cccttgggga aacagcccaa aggctgatcc agtcccaccc
     4081 tgaatctgca gaggacttaa aggaaaagtg cacagagtta aaccaggcct ggaccagcct
     4141 agggaagcgt gcagaccagc gcaaggccaa attgggtgac tcccatgatc tgcagcgctt
     4201 ccttagcgat ttccgggacc tcatgtcttg gatcaatgga atacgagggt tggtatcttc
     4261 agatgaacta gccaaggacg tcactggagc tgaggctttg ctggagcgac accaggaaca
     4321 ccggacagaa attgatgcca gggctggcac tttccaggca tttgagcagt ttgggcagca
     4381 gctgttagct cacgggcact atgccagccc ggagatcaaa gagaaacttg atattcttga
     4441 ccaggagcgc acagacctgg agaaggcctg ggtccagcgc aggatgatgc tggaccactg
     4501 cctggagttg cagctgttcc atcgggactg tgaacaagca gaaaactgga tggctgcccg
     4561 ggaggccttc ctaaacacag aagacaaagg agactcgctg gacagtgtgg aggcactgat
     4621 caaaaaacat gaagactttg acaaagctat caatgtccag gaagaaaaga tagctgccct
     4681 gcaggccttt gccgaccagc tcattgccgt tgaccactat gccaagggag acattgcaaa
     4741 ccgacgcaat gaggtccttg accggtggcg ccgcctaaaa gcccagatga ttgaaaaaag
     4801 gtcaaaactt ggagaatctc aaacacttca gcagttcagc cgggatgtgg atgagattga
     4861 agcctggatc agtgagaagt tacagacagc cagtgatgag tcatacaagg accccaccaa
     4921 catccagctt tccaagctgt tgagcaagca ccagaagcac caagcctttg aggcagaact
     4981 gcacgccaat gctgaccgaa tccgtggagt tatcgacatg ggcaactccc tcattgagcg
     5041 tggggcctgt gctggcagtg aggatgctgt caaggcccgc ctggctgccc ttgcagacca
     5101 gtggcagttc ctggtgcaga aatcagctga gaagagccag aagctaaaag aggcaaataa
     5161 gcagcagaac ttcaacactg gaatcaaaga ctttgacttc tggctctctg aggtggaggc
     5221 tctcctggca tcggaagatt atggcaaaga cctggcttct gtgaacaact tgctgaaaaa
     5281 gcatcagctg ctagaggcag acatctcggc ccacgaggat cgtctgaagg acctgaacag
     5341 ccaggctgac agcctgatga ctagcagtgc cttcgacacc tcccaagtga aagagaagcg
     5401 ggacaccatc aatggacgct tccagaagat caagagcatg gccacctccc gaagagcgaa
     5461 actgagcgag tcccaccgcc tgcaccagtt cttccgggac atggacgacg aggagtcctg
     5521 gatcaaggag aagaagttgt tagtgagctc tgaggactat ggcagagacc tcactggtgt
     5581 tcagaatctg aggaagaaac acaagcggct agaagctgaa ctggctgcac atgaacccgc
     5641 cattcagggt gtcctggaca ctgggaagaa gctgtctgat gacaacacca tcgggcagga
     5701 ggagatccag caacggcttg cacagtttgt ggagcactgg aaggaactaa agcagctggc
     5761 ggctgcgcgg ggccagcggc tagaggagtc cttggagtat cagcagtttg tagccaacgt
     5821 ggaagaggag gaggcttgga tcaatgagaa gatgactctg gtggccagcg aagactatgg
     5881 agacactctt gctgctatcc agggcttact gaagaaacac gaagcttttg agacagactt
     5941 cactgtccac aaggatcggg tgaatgatgt ctgtactaat ggacaagacc tcattaagaa
     6001 gaacaatcac catgaggaga acatctcttc aaagatgaag ggtctgaatg gaaaggtgtc
     6061 tgatctggag aaagcagcag ctcagaggaa agcaaagctg gatgagaact cagccttcct
     6121 tcagttcaac tggaaggctg acgtggtaga gtcctggatt ggtgaaaagg agaacagctt
     6181 gaaaacagat gactatggcc gagatctgtc ttctgtccaa actctgctca caaaacagga
     6241 gacatttgat gctggcctgc aggccttcca gcaggagggt attgccaata tcactgccct
     6301 caaagaccag ctgctagctg ccaagcacat tcagtctaag gccatcgagg cccgacatgc
     6361 ctccctcatg aagaggtgga cccagctgtt ggccaattca gctacccgga agaagaagtt
     6421 gctggaggcc cagagtcatt tccgtaaggt agaagacctc ttcctgacct ttgccaaaaa
     6481 ggcatcggct ttcaatagct ggtttgagaa tgcagaggaa gacctcacag acccagtgcg
     6541 ctgcaactct ctggaagaaa tcaaagccct tcgagaggct cacgatgcct tccgctcatc
     6601 cctcagctct gcacaggctg acttcaacca gctagccgag ctggaccgcc agatcaagag
     6661 tttccgggtg gcctccaacc cctatacctg gttcacgatg gaggccctgg aagagacctg
     6721 gagaaacctc cagaagatca ttaaggaacg agaactggag ctgcagaagg aacagcggcg
     6781 gcaggaggag aatgacaagc tgcgccaaga atttgcccag catgccaacg ccttccacca
     6841 gtggatccag gaaacaagaa cgtatctcct cgacgggtcc tgcatggtgg aagagtcggg
     6901 aactctggaa tctcagcttg aagctaccaa acgcaagcac caggagattc gagccatgag
     6961 aagtcagctg aagaagatcg aggacctggg ggctgccatg gaggaagccc tcatcctgga
     7021 caacaagtac acggagcaca gcactgtggg cctggcccag cagtgggacc agttagacca
     7081 gctgggcatg cgcatgcagc acaacctgga gcagcagatc caggccagga acacaacagg
     7141 agtgactgag gaggccctca aggaattcag catgatgttc aaacacttcg acaaggacaa
     7201 gtctggccgg ctgaaccatc aggagttcaa atcctgtctt cgctccctgg gctatgatct
     7261 gccaatggtt gaggaaggag agcctgatcc tgagtttgag gcaattctgg acactgttga
     7321 tcccaacagg gatggccacg tctccctgca agaatacatg gctttcatga tcagccgcga
     7381 aacggagaat gtcaagtcca gtgaagagat cgagagtgct ttccgggccc tcagctctga
     7441 gggcaagcct tatgtgacca aggaggagct ctaccagaac ctgacccggg aacaagctga
     7501 ctattgtgtc tcccacatga agccctatgt ggatggcaag ggccgagaac ttccaactgc
     7561 cttcgactac gtcgagttca cccgctcact ctttgtgaat tgatcaatcc tcttggcttg
     7621 taggcaagcc tgacctacac tgcttgccct gttatctcta tctatctatc acctttgctg
     7681 catgtcactt cctctgcttt caaggagaat attccagaca gtgttacttt ccagtgtaac
     7741 cataagcctg cttagcttgg ggtaagactt agtagaaaat ggtgcttcaa taaaccgctt
     7801 ccagtcacaa tcaccaatgt tgtgggaccc agacccatgt ttgagccagc tgccctcatt
     7861 cccacttaaa tcaaagcagc tggctcagct cccttgtttc tcttcccaaa tcctgttttc
     7921 atgtaaaaga aaaataaagg actcatttcc cctctaaaaa aaaaaaaaaa aaaaaaaaa
//
