LOCUS       NM_011465               8405 bp    mRNA    linear   ROD 06-NOV-2024
DEFINITION  Mus musculus spectrin alpha, erythrocytic 1 (Spta1), mRNA.
ACCESSION   NM_011465
VERSION     NM_011465.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   Vallelian,F., Buzzi,R.M., Pfefferle,M., Yalamanoglu,A.,
            Dubach,I.L., Wassmer,A., Gentinetta,T., Hansen,K., Humar,R.,
            Schulthess,N., Schaer,C.A. and Schaer,D.J.
  TITLE     Heme-stress activated NRF2 skews fate trajectories of bone marrow
            cells from dendritic cells towards red pulp-like macrophages in
            hemolytic anemia
  JOURNAL   Cell Death Differ 29 (8), 1450-1465 (2022)
   PUBMED   35031770
REFERENCE   3  (bases 1 to 8405)
  AUTHORS   Hale,J., An,X., Guo,X., Gao,E., Papoin,J., Blanc,L., Hillyer,C.D.,
            Gratzer,W., Baines,A. and Mohandas,N.
  TITLE     alphaI-spectrin represents evolutionary optimization of spectrin
            for red blood cell deformability
  JOURNAL   Biophys J 120 (17), 3588-3599 (2021)
   PUBMED   34352252
REFERENCE   4  (bases 1 to 8405)
  AUTHORS   Pfefferle,M., Ingoglia,G., Schaer,C.A., Yalamanoglu,A., Buzzi,R.,
            Dubach,I.L., Tan,G., Lopez-Cano,E.Y., Schulthess,N., Hansen,K.,
            Humar,R., Schaer,D.J. and Vallelian,F.
  TITLE     Hemolysis transforms liver macrophages into antiinflammatory
            erythrophagocytes
  JOURNAL   J Clin Invest 130 (10), 5576-5590 (2020)
   PUBMED   32663195
REFERENCE   5  (bases 1 to 8405)
  AUTHORS   Garcia-Caballero,A., Zhang,F.X., Hodgkinson,V., Huang,J., Chen,L.,
            Souza,I.A., Cain,S., Kass,J., Alles,S., Snutch,T.P. and
            Zamponi,G.W.
  TITLE     T-type calcium channels functionally interact with spectrin
            (alpha/beta) and ankyrin B
  JOURNAL   Mol Brain 11 (1), 24 (2018)
   PUBMED   29720258
  REMARK    GeneRIF: These findings reveal spectrin (alpha/beta) / ankyrin B
            cytoskeletal and signaling proteins as key regulators of T-type
            calcium channels expressed in the nervous system.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8405)
  AUTHORS   Oakey,R.J., Watson,M.L. and Seldin,M.F.
  TITLE     Construction of a physical map on mouse and human chromosome 1:
            comparison of 13 Mb of mouse and 11 Mb of human DNA
  JOURNAL   Hum Mol Genet 1 (8), 613-620 (1992)
   PUBMED   1301170
REFERENCE   7  (bases 1 to 8405)
  AUTHORS   Peters,L.L., White,R.A., Birkenmeier,C.S., Bloom,M.L., Lux,S.E. and
            Barker,J.E.
  TITLE     Changing patterns in cytoskeletal mRNA expression and protein
            synthesis during murine erythropoiesis in vivo
  JOURNAL   Proc Natl Acad Sci U S A 89 (13), 5749-5753 (1992)
   PUBMED   1385865
REFERENCE   8  (bases 1 to 8405)
  AUTHORS   Iolascon,A., Miraglia del Giudice,E. and Camaschella,C.
  TITLE     Molecular pathology of inherited erythrocyte membrane disorders:
            hereditary spherocytosis and elliptocytosis
  JOURNAL   Haematologica 77 (1), 60-72 (1992)
   PUBMED   1356891
  REMARK    Review article
REFERENCE   9  (bases 1 to 8405)
  AUTHORS   Gough,N.M., Lipkowitz,S., Kirsch,I.R. and Begley,C.G.
  TITLE     Localization of murine HLH gene NSCL to chromosome 1 by use of
            recombinant inbred strains
  JOURNAL   Mamm Genome 3 (3), 182-183 (1992)
   PUBMED   1617226
REFERENCE   10 (bases 1 to 8405)
  AUTHORS   Sassa,S. and Bernstein,S.E.
  TITLE     Studies of erythrocyte protoporphyrin in anemic mutant mice: use of
            a modified hematofluorometer for the detection of heterozygotes for
            hemolytic disease
  JOURNAL   Exp Hematol 6 (5), 479-487 (1978)
   PUBMED   658175
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY005246.1, AF093576.1 and AK143823.1.
            
            On Mar 27, 2010 this sequence version replaced NM_011465.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF093576.1, U87455.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849388, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-92                BY005246.1         1-92
            93-7708             AF093576.1         1-7616
            7709-8405           AK143823.1         2377-3073
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 80.97 cM"
     gene            1..8405
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /note="spectrin alpha, erythrocytic 1"
                     /db_xref="GeneID:20739"
                     /db_xref="MGI:MGI:98385"
     exon            1..186
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     CDS             166..7413
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /note="spectrin alpha chain, erythrocyte; erythroid
                     alpha-spectrin; spherocytosis; hemolytic anemia;
                     alpha-spectrin 1, erythroid; spectrin alpha 1;
                     neuroscience mutagenesis facility, 4"
                     /codon_start=1
                     /product="spectrin alpha chain, erythrocytic 1"
                     /protein_id="NP_035595.2"
                     /db_xref="CCDS:CCDS35795.1"
                     /db_xref="GeneID:20739"
                     /db_xref="MGI:MGI:98385"
                     /translation="METPKETAVESSGPKVLETAEEIQHRRAEVLNQYQRFKDRVAER
                     GQKLEESYHYQVFRRDADDLEKWIMEKLEIAKDKTYEPTNIQGKYQKHESFVSEVQAK
                     SRVLPELEEIREARFAEDHFAHEATKTHLKQLRLLWDLLLELTQEKSDVLLRALKFYQ
                     YSQECEDILEWVKEKEAIVTLVELGDDWERTEVLHKKFEEFQEELTARKGKVDRVNQY
                     ANECAQEKHPKLPEIKAKQDEVNAAWDRLWSLALKRRESLSNAADLQRFKRDVNEAIQ
                     WMEEKEPQLTSEDYGKDLVSSEALFHNHKRLERNLAVMDDKVKELCAKADKLMISHSA
                     DAPQIQQMKLDLVSNWERIRALATNRYAKLKASYGYHRFLSDYDELSGWMKEKTALIN
                     ADELPTDVASGEALLARHQQHKHEIDSYDDRFQSADATGQELLDGNHEASEEIREKMT
                     ILANDWAALLELWDKCQHQYRQCLDFHLFYRDSEQVDSWMSRQEAFLENEDLGNSVGS
                     VEALLQKHDDFEEAFTAQEEKIITLDETATKLIDNDHYDSENIAAIRDGLLARRDALR
                     ERAATRRKLLVDSQLLQQLYQDSDDLKTWINKKKKLADDDDYKDVQNLKSRVQKQQDF
                     EEELAVNEIMLNNLEKTGQEMIEDGHYASEAVAARLSEVANLWKELLEATAQKGTQLY
                     EANQLLQFENNAEDLKRWLEEVEWQVTSEDYGKGLADVQNLLRKHGLLESDVTARQNQ
                     VDTLTDMAAHFEEIGHPDSGDIRARQESLLSRFEALKEPLAIRKKKLIDLLKLQQICR
                     DSEDEEAWIQETEPSAASTHLGKDLVAAKNLLNRHEVILADIASHEPRIQVITERGNK
                     MVEEGHFAAEDIASRVESLNKNMESLHARAIRRENDLKANVQLQQYLADLHEAEAWIK
                     EKEPIVDNKNYGADEEAAGALLKKHEAFLVDLNAFENSIKALRDQAEVCQQQQAAPVD
                     EAGREARVIALYDFEARSRREVSMKKNDVLTLLSSINKDWWKVEADDHQGFVPAVYVR
                     KLAPDELPGFPQHRQEEPVNIPQLQQQVETLYHSLLDRAEERRRRLLQRYNEFLLAYE
                     AGDMLEWIQEKKTENTGVELDDVWELQKKFDEFQRDLKSNEPRLKDINKVADELLFEE
                     LLTPEGAHIRQELNTRWNSLKRLADEQYQLLSSAHAVEMFHREADDVKEQIDKKCRAL
                     NAADPGSDLLSVQALQRQHEVFERDIIPLGEKVTTLGETAERLCESHPDATEDLQKQR
                     TELNEAWDTLQGLTSDRKESLNEAHKFFLFLSKASDLENWIKTIGGVISSPELAEDLT
                     GTEILLERHQEHHDDIKREDPTFQALEDFGTELIDSGHRNRREIDNTLQNINSKRDNL
                     EKSWENRKKMLDQCLELQLFRGKCDQVESWMVARENSLRSDDRDHLNSLQALMKKRDD
                     LDKAITAQEGKISDLENVATRLIDNDHYAKEEIAARLQRVLDRWKALKEQLLTELGKL
                     GDYADLKQFYRDLEDLEEWINEMLPIACDESYKDPTNIQRKYLKHQAFENEVNGRAEQ
                     VDGVINLGNSLIERRVCDGDEENMQEQLDKLKENWDYLLERTTDKGQKLNEASRQQRF
                     NTSIRDFEFWLSEAEGLLAMKDQARDLTSAGNLLKKHQLLEAEMLAREDPLKDLNDLA
                     QELISSGTFNIDQIEEKMNGVNERFENVQSLAAAHHEKLKETYALFQFFQDLDDEEAW
                     IEEKLLRVSSQDYGRDLQSVQNLLKKHKRLEGELVAHEPAVQNVLDTAESLRDKAAVG
                     KEEIQERLAQFVQHWEKLKELAKTRGVNLEESLEYLQFMENAEEEEAWLGEKCALVSR
                     GDSGDTLAATQSLLKKHEALENDFAVHKNRVQDVCAQGEDILNKEETQNKDKISTKIQ
                     VLNEKTASLAKALAAWKSQLDDVHAFQQFNWKADVVESWIGEKEASLKTKSNGADLTA
                     FLTLLAKHDTLDASLQSFQQERLSEIAELKDQLVAGEHSQAKAIEEQHAALLRHWEQL
                     LEASRVHRQKLLEKQLPLQKAEELFMEFAHKASAFNNWCENAEEDLSEPVHCVSLNEI
                     RQLQKEHEAFLASLAGAQEDFNYLLELDKQIKALNVPSSPYTWLTVDVLGRIWNHLPD
                     IIKEREQELQKEEARQIKNFEMCQEFEQNASAFLQWIQETRAYFLDGSLLKETGTLES
                     QLEANKRKQKEIQAMKRHLTKIEDLGDSMEEALILDIKYSTIGLAQQWDQLHQLGMRM
                     QHNLEQQIQAKDTIGVSEETLKEFSTTYKHFDENLTGRLTHKEFRSCLRGLNYYLPMV
                     EEGEPEPKFEKFLNAVDPGRKGYVSLEDYTSFLIDKESENIKTSDDIESAFQALAEGK
                     AYITKEDMKQALTPEQVSFCTIHMQQYMDPRGRSQPAGYDYVGFTNSFFGN"
     exon            187..423
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            424..549
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            550..690
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            691..837
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            838..971
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1116
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            1117..1271
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1407
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1509
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            1510..1647
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            1648..1758
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1836
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..1992
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2197
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            2198..2379
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            2380..2623
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            2624..2746
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            2747..2964
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            2965..3057
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            3058..3195
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            3196..3347
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            3348..3534
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            3535..3636
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            3637..3728
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            3729..3873
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            3874..4055
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            4056..4155
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            4156..4353
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            4354..4497
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            4498..4601
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            4602..4764
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            4765..4896
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            4897..5034
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            5035..5139
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            5140..5348
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            5349..5469
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            5470..5591
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            5592..5724
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            5725..5823
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            5824..5986
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            5987..6063
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            6064..6273
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            6274..6570
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            6571..6683
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            6684..6701
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            6702..6753
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            6754..6941
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            6942..6995
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            6996..7142
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            7143..7287
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     exon            7288..8405
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8003..8008
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /note="hexamer: ATTAAA"
     polyA_site      8024
                     /gene="Spta1"
                     /gene_synonym="ha; ihj; nmf4; sph; Spna-1; Spna1"
                     /note="major polyA site"
ORIGIN      
        1 gattgtctac gcttggcggg actctaaaac tctttttttc ctctgggcct ttgccacatt
       61 cacatttctg acattcctat ttgaagtgga ctggacattc aaagaactat ttttctctct
      121 tagctacatt tcatcttgca aagccttaga ctgagctgag cagagatgga aactccaaag
      181 gaaactgctg tcgagagcag cggaccaaag gtcctagaaa cagcagaaga aatccaacac
      241 cgaagagcgg aggtgttaaa ccagtaccag aggttcaagg atcgcgttgc cgagaggggt
      301 cagaagcttg aggagtctta ccactatcaa gttttcagga gagacgcaga tgacctggag
      361 aagtggatca tggaaaagct tgaaattgca aaagataaga catatgaacc cacaaacata
      421 caggggaaat atcagaagca cgaatccttt gtatcagagg tgcaagccaa atcaagagtc
      481 cttcctgagc tggaggaaat cagggaagct cgatttgcag aagaccattt tgcccatgaa
      541 gccaccaaga cccatctgaa gcaacttcgc ctgctgtggg atctgctctt ggagttgacc
      601 caagagaaaa gtgacgtgct gctgcgggca ctgaagttct atcaatattc tcaggagtgt
      661 gaagacatcc tagagtgggt caaagagaag gaagctatag tgacattagt ggagctgggc
      721 gatgactggg agcgaactga ggtcctgcat aagaagtttg aagaattcca ggaagaactg
      781 accgctcgga agggaaaagt ggacagagtg aaccaatatg ctaatgagtg tgcccaggag
      841 aaacatccta agttaccaga gataaaggca aaacaggatg aagtcaatgc tgcctgggat
      901 cgtctctgga gcctggctct caagagacga gaaagtctgt ctaatgcagc agacctccag
      961 cgatttaaaa gggatgtcaa tgaagctatc cagtggatgg aggagaagga gccgcagctc
     1021 acctctgagg actatggcaa agatcttgtt agttctgagg cattgtttca caatcacaag
     1081 aggctcgaga ggaatctcgc ggtcatggat gacaaggtga aggagttgtg tgccaaagca
     1141 gacaagctga tgatttccca ttctgcagat gcacctcaga ttcaacagat gaaattggat
     1201 ctagtctcca actgggaacg catccgtgcc ttggccacta acagatatgc aaagctgaag
     1261 gcttcttatg ggtaccatcg gttcttatct gactatgatg agctctcagg ctggatgaag
     1321 gaaaagactg ctctgatcaa tgctgatgag ctgccaacag atgtggccag tggagaagcc
     1381 ctgctagcca ggcaccagca gcataagcat gagattgact cttatgatga tcgatttcaa
     1441 tctgctgatg caactggtca agaactcctg gatggcaatc acgaagcttc tgaggaaatt
     1501 cgagagaaga tgacaatact tgccaatgac tgggctgctc tgctggaact gtgggacaag
     1561 tgtcagcacc aataccgcca atgtctggat ttccatctct tctaccgaga cagtgaacaa
     1621 gtggatagtt ggatgagcag acaagaggct ttcctggaaa atgaagacct ggggaactca
     1681 gtgggcagcg tggaagccct ccttcagaag catgatgact ttgaagaagc gttcactgcc
     1741 caggaagaaa agatcataac cttggatgag actgcaacca agcttattga caatgaccac
     1801 tatgactctg agaatatcgc tgctattcgt gatgggcttt tggccagacg tgatgccctg
     1861 cgtgaaaggg ctgccactag aagaaaattg ttggtagatt cacaacttct tcaacaactg
     1921 tatcaagact cagatgacct aaaaacctgg atcaataaga agaaaaagtt ggctgatgat
     1981 gacgattaca aggatgtgca gaacttgaag agccgggttc agaagcagca ggactttgaa
     2041 gaggaattgg cagtgaatga gattatgctc aataacttgg agaaaacagg ccaggagatg
     2101 attgaggatg gtcactacgc ctctgaggct gtggctgctc gcctgagtga ggttgccaac
     2161 ctctggaagg agttgctgga ggcaacagca caaaaaggga ctcagctgta tgaagctaac
     2221 cagctgctac agtttgagaa caatgcagaa gacctgaagc gctggctaga ggaggtggag
     2281 tggcaggtta cctctgagga ttatgggaaa ggtctggctg atgtacagaa tctactaaga
     2341 aaacatggcc ttctagagtc agatgtgact gctcgtcaga atcaggtgga caccctcaca
     2401 gacatggcgg cacactttga agagataggc catcccgact ctggggacat ccgtgcaagg
     2461 caggaatcct tgctttcccg ttttgaggct ctgaaggagc cactggccat tcggaagaag
     2521 aagctcattg acctactcaa gctacagcag atttgcagag actcggaaga tgaggaggcg
     2581 tggatccagg agactgagcc ctcagcagct tccactcatc ttggaaagga cttggttgca
     2641 gccaagaatc ttctaaacag acatgaagtc atcctggcag acattgcgag tcatgaacca
     2701 cggattcaag taataacaga gaggggaaac aaaatggtgg aggaaggaca cttcgctgca
     2761 gaagacatag catctagggt tgaaagcttg aacaaaaata tggagtctct ccatgctcga
     2821 gctattagac gggagaatga tcttaaggcc aatgtccagc tccaacagta cctggcagat
     2881 ctgcatgaag cagaagcatg gattaaagag aaagaaccca ttgtagacaa caaaaactat
     2941 ggagctgatg aagaagcagc tggggccctt ctcaagaagc atgaagcctt cctggtggat
     3001 ctgaatgcat ttgaaaacag cataaaggct ctaagagatc aggcagaagt ctgccagcaa
     3061 cagcaggcag caccagtgga cgaggctggt cgagaagcta gggtcatagc tttgtatgac
     3121 tttgaggctc gcagccgtag agaggtcagc atgaagaaga atgatgtctt aactctgctc
     3181 agttccatca acaaggactg gtggaaggtt gaagctgatg atcaccaggg ctttgtgcca
     3241 gcggtttatg tcaggaaact ggcccccgat gagctaccag gtttcccaca gcatcggcaa
     3301 gaggagccag tcaacatccc tcagctccag cagcaggtcg agaccctgta ccactccctc
     3361 ctagatcggg cagaagagcg cagacgtcgc ttgctgcagc gctacaatga gtttctgctg
     3421 gcctatgagg caggagacat gttggagtgg atccaagaga aaaagacaga aaacactgga
     3481 gtggaactgg atgatgtttg ggaactgcag aagaagtttg acgagttcca gagggatcta
     3541 aagagtaatg agcctcggct aaaggacatc aacaaagtgg ctgacgaact gctctttgaa
     3601 gagctcctaa ccccagaagg agctcacatt cgacaggagt tgaacacccg ctggaattcc
     3661 ctgaagaggc tggcagatga acagtatcag ctgctcagca gtgcacatgc tgttgagatg
     3721 tttcacaggg aagcagatga tgtcaaggaa cagattgata agaaatgccg ggctctcaat
     3781 gctgccgacc ctggctctga cctgctcagt gtccaggccc tccagaggca acatgaagtc
     3841 tttgagagag acatcatccc cctgggagaa aaggtgacta cgctggggga gactgctgag
     3901 cgtctctgtg aatcacaccc agatgccact gaggacctac agaagcagag aacagagctg
     3961 aatgaggcct gggacacact tcaaggtctt acaagtgatc ggaaggagag tctgaatgaa
     4021 gcccataaat tcttcctctt ccttagcaag gccagtgatc tagagaactg gatcaaaacc
     4081 attggcggtg tgatatcatc acctgagctg gctgaggact taactggcac agagatcttg
     4141 ctggagcgac accaggagca tcacgatgac ataaagagag aggaccctac cttccaggcc
     4201 ttagaagact ttggtacaga acttatagac agcggccacc gtaacagacg tgaaattgac
     4261 aatactctcc agaatattaa ttcaaagaga gacaatttgg aaaagagttg ggaaaaccgc
     4321 aagaagatgc tagaccagtg cttagaactg cagttattcc gaggaaagtg tgaccaggtc
     4381 gagagctgga tggtggcccg tgagaattcc ctgaggtcag acgacaggga tcacttaaac
     4441 agtctgcagg ctttgatgaa gaaacgggat gatttggaca aagcaatcac tgcccaggaa
     4501 gggaagattt cggacctaga gaacgttgcc acaaggctca tagacaatga ccactatgcc
     4561 aaggaagaga ttgctgctcg cctccaacgt gtgctagaca ggtggaaggc tctcaaagaa
     4621 cagctgctta ctgaactggg aaagctagga gactatgctg acctcaaaca gttctatcgt
     4681 gaccttgagg acctggagga atggatcaat gagatgcttc ccatagcctg tgatgaatct
     4741 tataaagatc ccactaacat tcagaggaaa tatctgaagc atcaagcctt tgaaaatgaa
     4801 gtcaacggtc gtgctgagca ggtggatggg gttatcaacc ttgggaattc cctgattgag
     4861 cgcagagtgt gtgatggaga tgaagagaac atgcaggagc aactggacaa gctaaaggag
     4921 aactgggatt atctcctaga gagaaccact gacaaaggac agaagctcaa cgaggccagt
     4981 cggcagcaaa ggttcaacac aagcatccgg gactttgagt tctggctttc agaggcagaa
     5041 ggcttgttgg ccatgaagga tcaggccagg gacttaactt cagcaggaaa cctacttaag
     5101 aagcatcagc tattggaagc agagatgttg gcacgagagg accctctcaa agatctgaat
     5161 gacctggctc aggagctgat ctccagtggg actttcaaca ttgaccagat agaagagaaa
     5221 atgaatggtg tcaatgagcg ctttgaaaat gtccagagct tggcagctgc acaccacgag
     5281 aagctgaaag agacgtatgc cttgttccag ttcttccaag acctggatga tgaggaagcc
     5341 tggatagagg agaagttgtt acgagtgagc tcccaggact atgggaggga tcttcagagt
     5401 gttcagaact tattgaaaaa acacaaacgc ctagaggggg agctagtggc acatgaacct
     5461 gctgtccaga atgtgctgga tacggcagaa agcctgagag acaaggctgc tgtagggaaa
     5521 gaggagatcc aggagcggct ggctcagttt gtccaacact gggaaaagct caaagagttg
     5581 gcgaagaccc gaggggtgaa cttggaggag tccctcgagt atctgcagtt catggagaat
     5641 gcagaagaag aggaagcatg gctgggggag aagtgtgctc tggtcagtcg cggggattca
     5701 ggagacacac tggctgctac tcagagtttg ctaaagaagc atgaagcttt ggagaatgac
     5761 tttgctgtgc acaagaaccg ggtacaagat gtttgtgcac aaggagaaga cattcttaat
     5821 aaggaagaaa ctcagaacaa ggataagatt tccaccaaga tccaagttct gaatgaaaag
     5881 acagcctctt tggccaaggc attagctgct tggaagtcac aactggacga tgtgcatgcc
     5941 tttcagcagt ttaactggaa ggcggacgta gtggaatcct ggataggtga gaaagaagca
     6001 agcctgaaga ccaagagcaa cggtgcagac ctcaccgcct tcctcacact actggcaaag
     6061 cacgacacac tggatgccag tcttcagagc ttccagcaag aaaggctttc tgaaatcgct
     6121 gagctgaagg accagcttgt ggctggtgag cacagccaag ccaaagccat tgaggagcag
     6181 catgcagcct tgctgaggca ctgggaacaa ctgctggagg catctagagt tcacaggcag
     6241 aaactgctgg aaaagcagct gcctctacag aaggctgagg agctattcat ggaatttgca
     6301 cacaaagctt cagccttcaa caactggtgt gagaacgcgg aagaggactt gtcagaacct
     6361 gtgcattgtg tctctctaaa tgagatccgg cagctgcaga aggaacatga agccttcttg
     6421 gcctctctgg ctggggctca agaggatttc aactatttgt tggagctaga caagcaaatt
     6481 aaagccttaa atgtcccctc cagtccttat acctggttaa cagtggatgt gctgggcaga
     6541 atctggaacc acctgccaga catcatcaag gaaagggaac aggaactaca gaaagaagag
     6601 gctaggcaga tcaagaactt tgaaatgtgc caggagtttg aacaaaatgc cagtgccttc
     6661 cttcagtgga tccaggagac cagggcctat tttctggatg gatcattgct caaggaaaca
     6721 ggaactctgg aatcccaact tgaagcaaat aaaaggaaac agaaggagat ccaggcaatg
     6781 aagcgccatc tgaccaagat tgaagacctg ggggacagca tggaagaggc tctgatcctt
     6841 gatatcaaat acagcaccat tggactggcc cagcaatggg atcagctgca ccaactaggg
     6901 atgcgaatgc aacacaattt ggagcaacag attcaagcca aagacaccat aggtgtgagt
     6961 gaggagacat tgaaagaatt tagcaccaca tacaaacact tcgatgagaa cttgacaggg
     7021 cgattgactc acaaagagtt ccgctcttgc ctgagaggac tcaattacta tctgcctatg
     7081 gtagaggaag gtgaacctga gcccaagttt gagaaattcc tgaatgctgt ggatccaggg
     7141 aggaaaggat atgtttcact ggaagactat acatcattcc tgattgacaa ggagtcagag
     7201 aatatcaaga ccagcgatga catagagagt gcattccaag ctctggcaga aggcaaggcc
     7261 tatattacca aggaggacat gaaacaggct ctcaccccgg aacaagtgtc attctgtacc
     7321 atacatatgc agcaatatat ggatccacgg ggtcggagcc aacctgctgg ctacgactat
     7381 gttggcttca ccaattcatt ctttggcaat tgatatgcag attcttatgc atcatagaaa
     7441 atcatagtgt ggcagcaagt aatgggggat aagaggcaca agcaagcgtg aaagaaaaaa
     7501 agatcctgct gctctgtgtg tgtgtgtgtt acatttattg tgggattttt aaaaaaataa
     7561 tcaagtatat agcgggggct aagaaaaatc tataggatta tgtaactagt ctctacctag
     7621 ggtgccaaga ttttaatgtg tgtgtgtgtg tgtgtgtatg tgtatgtgtg tgtgtgctgc
     7681 acacctttaa gattttactg cattctgatt atagcagaaa tatttctttt ttaaagggaa
     7741 atttaggtaa aattatgctt ttgtattttt ggaaccatat tattactaaa tataatatgc
     7801 aattaattat actgtggaaa aggacaataa taaattagat aaataaaata caaggtgatt
     7861 cacagtttgt tagtagcttt taagagagat gaaatcctta caatttgaaa tgtttgtaat
     7921 tttgtcttat tctatgaagt agagtcagaa aaagataaca taaaatgctt gttttcttct
     7981 tgtcctgttt tgatatttca ccattaaagt gaatgacttt ggcatgtcag ggtcctctca
     8041 tgttccctaa atctctgaat actctcttac attttaatat gatacaactg atgttttatt
     8101 catttctatc ttctcagaag ccagtaaaat gttcataatt taaggaaaaa accaagaagc
     8161 tatggtggta ctatatctag gtgttttcag tacaggtatg tttcttaggt aactttgttg
     8221 ccttttgtga aatttagcct ttcgtacaga tagcctattg ctcatgaata tcactcctta
     8281 tttactatct taagtctcag ttttttcgct tgagtagaaa ttaacccctc ctcaatcttt
     8341 gggaaaaaat tctcacagga tctaattaaa atacttatgg ctacaaataa agatatttga
     8401 aaatg
//
