LOCUS       NM_013539               1217 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus splA/ryanodine receptor domain and SOCS box containing
            2 (Spsb2), transcript variant 1, mRNA.
ACCESSION   NM_013539
VERSION     NM_013539.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Li,K., You,T., Zhao,P., Luo,Y., Zhang,D., Wei,H., Wang,Y., Yang,J.,
            Guan,X. and Kuang,Z.
  TITLE     Structural basis for the regulation of inducible nitric oxide
            synthase by the SPRY domain-containing SOCS box protein SPSB2, an
            E3 ubiquitin ligase
  JOURNAL   Nitric Oxide 113-114, 1-6 (2021)
   PUBMED   33862200
  REMARK    GeneRIF: Structural basis for the regulation of inducible nitric
            oxide synthase by the SPRY domain-containing SOCS box protein
            SPSB2, an E3 ubiquitin ligase.
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Pfeifer,M., Brem,R., Lippert,T.P., Boulianne,B., Ho,H.N.,
            Robinson,M.E., Stebbing,J. and Feldhahn,N.
  TITLE     SSB1/SSB2 Proteins Safeguard B Cell Development by Protecting the
            Genomes of B Cell Precursors
  JOURNAL   J Immunol 202 (12), 3423-3433 (2019)
   PUBMED   31085591
  REMARK    GeneRIF: these results establish SSB1/2 as safeguards of B cell
            development and unveil their differential requirement in immature
            and mature B lymphocytes
REFERENCE   3  (bases 1 to 1217)
  AUTHORS   Shi,W., Vu,T., Boucher,D., Biernacka,A., Nde,J., Pandita,R.K.,
            Straube,J., Boyle,G.M., Al-Ejeh,F., Nag,P., Jeffery,J.,
            Harris,J.L., Bain,A.L., Grzelak,M., Skrzypczak,M., Mitra,A.,
            Dojer,N., Crosetto,N., Cloonan,N., Becherel,O.J., Finnie,J.,
            Skaar,J.R., Walkley,C.R., Pandita,T.K., Rowicka,M., Ginalski,K.,
            Lane,S.W. and Khanna,K.K.
  TITLE     Ssb1 and Ssb2 cooperate to regulate mouse hematopoietic stem and
            progenitor cells by resolving replicative stress
  JOURNAL   Blood 129 (18), 2479-2492 (2017)
   PUBMED   28270450
  REMARK    GeneRIF: Collectively, Ssb1 and Ssb2 control crucial aspects of
            Hematopoietic stem and progenitor cells function, including
            proliferation and survival in vivo by resolving replicative stress
            to maintain genomic stability.
REFERENCE   4  (bases 1 to 1217)
  AUTHORS   Yap,B.K., Leung,E.W., Yagi,H., Galea,C.A., Chhabra,S.,
            Chalmers,D.K., Nicholson,S.E., Thompson,P.E. and Norton,R.S.
  TITLE     A potent cyclic peptide targeting SPSB2 protein as a potential
            anti-infective agent
  JOURNAL   J Med Chem 57 (16), 7006-7015 (2014)
   PUBMED   25068993
REFERENCE   5  (bases 1 to 1217)
  AUTHORS   Lewis,R.S., Kolesnik,T.B., Kuang,Z., D'Cruz,A.A., Blewitt,M.E.,
            Masters,S.L., Low,A., Willson,T., Norton,R.S. and Nicholson,S.E.
  TITLE     TLR regulation of SPSB1 controls inducible nitric oxide synthase
            induction
  JOURNAL   J Immunol 187 (7), 3798-3805 (2011)
   PUBMED   21876038
REFERENCE   6  (bases 1 to 1217)
  AUTHORS   Yao,S., Liu,M.S., Masters,S.L., Zhang,J.G., Babon,J.J.,
            Nicola,N.A., Nicholson,S.E. and Norton,R.S.
  TITLE     Dynamics of the SPRY domain-containing SOCS box protein 2:
            flexibility of key functional loops
  JOURNAL   Protein Sci 15 (12), 2761-2772 (2006)
   PUBMED   17088318
  REMARK    GeneRIF: Backbone dynamics of SSB-2 by means of Modelfree analysis.
REFERENCE   7  (bases 1 to 1217)
  AUTHORS   Masters,S.L., Yao,S., Willson,T.A., Zhang,J.G., Palmer,K.R.,
            Smith,B.J., Babon,J.J., Nicola,N.A., Norton,R.S. and Nicholson,S.E.
  TITLE     The SPRY domain of SSB-2 adopts a novel fold that presents
            conserved Par-4-binding residues
  JOURNAL   Nat Struct Mol Biol 13 (1), 77-84 (2006)
   PUBMED   16369487
  REMARK    GeneRIF: Determined the first SPRY-domain structure of SSB-2 that
            adopts a novel fold and contains conserved structural determinants
            for binding to prostate apoptosis response protein-4 (PAR-4).
REFERENCE   8  (bases 1 to 1217)
  AUTHORS   Masters,S.L., Palmer,K.R., Stevenson,W.S., Metcalf,D., Viney,E.M.,
            Sprigg,N.S., Alexander,W.S., Nicola,N.A. and Nicholson,S.E.
  TITLE     Genetic deletion of murine SPRY domain-containing SOCS box protein
            2 (SSB-2) results in very mild thrombocytopenia
  JOURNAL   Mol Cell Biol 25 (13), 5639-5647 (2005)
   PUBMED   15964819
  REMARK    GeneRIF: Genetic deletion of SOCS Box Protein 2 results in a very
            mild thrombocytopenia.
REFERENCE   9  (bases 1 to 1217)
  AUTHORS   Kile,B.T., Schulman,B.A., Alexander,W.S., Nicola,N.A., Martin,H.M.
            and Hilton,D.J.
  TITLE     The SOCS box: a tale of destruction and degradation
  JOURNAL   Trends Biochem Sci 27 (5), 235-241 (2002)
   PUBMED   12076535
  REMARK    Review article
REFERENCE   10 (bases 1 to 1217)
  AUTHORS   Ansari-Lari,M.A., Oeltjen,J.C., Schwartz,S., Zhang,Z., Muzny,D.M.,
            Lu,J., Gorrell,J.H., Chinault,A.C., Belmont,J.W., Miller,W. and
            Gibbs,R.A.
  TITLE     Comparative sequence analysis of a gene-rich cluster at human
            chromosome 12p13 and its syntenic region in mouse chromosome 6
  JOURNAL   Genome Res 8 (1), 29-40 (1998)
   PUBMED   9445485
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC142254.4.
            
            On Jul 25, 2022 this sequence version replaced NM_013539.2.
            
            Summary: This gene encodes a member of the SSB family of proteins
            that contain a central SPRY (repeats in splA and ryanodine
            receptors) domain and a C-terminal SOCS (suppressor of cytokine
            signaling) box. The encoded protein is an adaptor protein in the E3
            ubiquitin ligase complex that ubiquitinates inducible nitric oxide
            synthase and targets it for proteasomal degradation. Mice lacking
            the encoded protein exhibit lower blood urea nitrogen levels and
            mild thrombocytopenia due to reduced platelet production.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Apr 2015].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1). Variants 1-4 and 6-7 all encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.153742.1,
                                           SRR13422598.550711.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-126               AC142254.4         125014-125139       c
            127-869             AC142254.4         124075-124817       c
            870-1217            AC142254.4         123428-123775       c
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 59.17 cM"
     gene            1..1217
                     /gene="Spsb2"
                     /gene_synonym="C9; Grcc9; SSB2"
                     /note="splA/ryanodine receptor domain and SOCS box
                     containing 2"
                     /db_xref="GeneID:14794"
                     /db_xref="MGI:MGI:1315199"
     exon            1..126
                     /gene="Spsb2"
                     /gene_synonym="C9; Grcc9; SSB2"
                     /inference="alignment:Splign:2.1.0"
     exon            127..869
                     /gene="Spsb2"
                     /gene_synonym="C9; Grcc9; SSB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             206..1000
                     /gene="Spsb2"
                     /gene_synonym="C9; Grcc9; SSB2"
                     /note="isoform 1 is encoded by transcript variant 1; SPRY
                     domain-containing SOCS box protein 2; gene-rich cluster
                     protein C9"
                     /codon_start=1
                     /product="SPRY domain-containing SOCS box protein 2
                     isoform 1"
                     /protein_id="NP_038567.1"
                     /db_xref="CCDS:CCDS20529.1"
                     /db_xref="GeneID:14794"
                     /db_xref="MGI:MGI:1315199"
                     /translation="MGQTALARGSSSTPTSQALYSDFSPPEGLEELLSAPPPDLVAQR
                     HHGWNPKDCSENIDVKEGGLCFERRPVAQSTDGVRGKRGYSRGLHAWEISWPLEQRGT
                     HAVVGVATALAPLQADHYAALLGSNSESWGWDIGRGKLYHQSKGLEAPQYPAGPQGEQ
                     LVVPERLLVVLDMEEGTLGYSIGGTYLGPAFRGLKGRTLYPSVSAVWGQCQVRIRYMG
                     ERRVEEPQSLLHLSRLCVRHALGDTRLGQISTLPLPPAMKRYLLYK"
     exon            870..1217
                     /gene="Spsb2"
                     /gene_synonym="C9; Grcc9; SSB2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1194..1199
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spsb2"
                     /gene_synonym="C9; Grcc9; SSB2"
                     /note="hexamer: AGTAAA"
     polyA_site      1217
                     /gene="Spsb2"
                     /gene_synonym="C9; Grcc9; SSB2"
                     /note="major polyA site"
ORIGIN      
        1 agtggctggt caggggcgct tctagccttc cctatctgta cttccacaga ggtctctgcg
       61 agctaggggg acagtgaggt gcggggtagg ggcccggcgt tagagccagc aaggggacgg
      121 ttcacgggga cgatccggag ctcaactttc aaaagcgaga cgccccagca agcctgtttt
      181 gagaagttct tcagcggctc tcctcatggg ccagacggcc ctggcaaggg gcagcagcag
      241 cacccctacc tcgcaggctc tgtactcgga cttctctcct cccgagggct tggaggagct
      301 cctgtctgct ccccctcctg acctggttgc ccaacggcac cacggctgga accccaagga
      361 ttgctccgag aacatcgatg tcaaggaagg gggtctgtgc tttgagcggc gccctgtggc
      421 ccagagcact gatggagtcc gggggaaacg gggctattcg agaggtctgc acgcctggga
      481 gatcagctgg cccctggagc aaaggggcac acacgccgtg gtgggcgtgg ccaccgccct
      541 cgccccgctg caggctgacc actatgcggc gcttttgggc agcaacagcg agtcctgggg
      601 ctgggatatt gggcggggaa aattgtatca tcagagtaag ggcctcgagg ccccccagta
      661 tccagctgga cctcagggtg agcagctagt ggtgccagag agactgctgg tggttctgga
      721 catggaggag gggactcttg gctactctat tgggggcacg tacctgggac cagccttccg
      781 tggactgaag gggaggaccc tctatccctc tgtaagtgct gtttggggcc agtgccaggt
      841 ccgcatccgc tacatgggcg aaagaagagt ggaggaacca caatcccttc tgcacctgag
      901 ccgcctgtgt gtgcgccatg ctctggggga cacccggctg ggtcaaatat ccactctgcc
      961 tttgccccct gccatgaagc gctatctgct ctacaaatga cccagtagta cagggtgtgc
     1021 tggcacccta ccgtggggac aggtggagag gcacccgctg gcctagacaa ctttaaaaag
     1081 ctggtgaagc tggggggggg gggctggacc ccttcacctc cccttctcac aggagcaaga
     1141 catatagaaa tgatattaaa caccatggca gcctgggaca aagaggtttt tgaagtaaaa
     1201 aatgagatgt attgtca
//
