LOCUS       NM_001111141            4456 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus SprT-like N-terminal domain (Sprtn), transcript
            variant 1, mRNA.
ACCESSION   NM_001111141 XM_001480455
VERSION     NM_001111141.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Erber,L., Groehler,A.S. 4th, Cyuzuzo,C.I., Baker-Wainwright,J.,
            Maskey,R.S., Li,L., Machida,Y.J. and Tretyakova,N.
  TITLE     SPRTN metalloprotease participates in repair of ROS-mediated
            DNA-protein crosslinks
  JOURNAL   Sci Rep 14 (1), 30919 (2024)
   PUBMED   39730693
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Huang,J., Zhou,Q., Gao,M., Nowsheen,S., Zhao,F., Kim,W., Zhu,Q.,
            Kojima,Y., Yin,P., Zhang,Y., Guo,G., Tu,X., Deng,M., Luo,K.,
            Qin,B., Machida,Y. and Lou,Z.
  TITLE     Tandem Deubiquitination and Acetylation of SPRTN Promotes
            DNA-Protein Crosslink Repair and Protects against Aging
  JOURNAL   Mol Cell 79 (5), 824-835 (2020)
   PUBMED   32649882
REFERENCE   3  (bases 1 to 4456)
  AUTHORS   Maskey,R.S., Flatten,K.S., Sieben,C.J., Peterson,K.L., Baker,D.J.,
            Nam,H.J., Kim,M.S., Smyrk,T.C., Kojima,Y., Machida,Y., Santiago,A.,
            van Deursen,J.M., Kaufmann,S.H. and Machida,Y.J.
  TITLE     Spartan deficiency causes accumulation of Topoisomerase 1 cleavage
            complexes and tumorigenesis
  JOURNAL   Nucleic Acids Res 45 (8), 4564-4576 (2017)
   PUBMED   28199696
  REMARK    GeneRIF: It has been shown that the livers of Sprtn deficient mice
            accumulate DNA-protein crosslinks containing Topoisomerase 1
            covalently linked to DNA.
REFERENCE   4  (bases 1 to 4456)
  AUTHORS   Lopez-Mosqueda,J., Maddi,K., Prgomet,S., Kalayil,S.,
            Marinovic-Terzic,I., Terzic,J. and Dikic,I.
  TITLE     SPRTN is a mammalian DNA-binding metalloprotease that resolves
            DNA-protein crosslinks
  JOURNAL   Elife 5, e21491 (2016)
   PUBMED   27852435
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4456)
  AUTHORS   Maskey,R.S., Kim,M.S., Baker,D.J., Childs,B., Malureanu,L.A.,
            Jeganathan,K.B., Machida,Y., van Deursen,J.M. and Machida,Y.J.
  TITLE     Spartan deficiency causes genomic instability and progeroid
            phenotypes
  JOURNAL   Nat Commun 5, 5744 (2014)
   PUBMED   25501849
  REMARK    GeneRIF: The data demonstrate that Spartan plays a key role in
            maintaining structural and numerical chromosome integrity and
            suggest a link between Spartan insufficiency and progeria.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC139158.3.
            
            On Jun 16, 2018 this sequence version replaced NM_001111141.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.1011420.1,
                                           SRR9219381.280394.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-488               AC139158.3         101934-102421       c
            489-588             AC139158.3         101140-101239       c
            589-717             AC139158.3         99980-100108        c
            718-985             AC139158.3         98423-98690         c
            986-4456            AC139158.3         94154-97624         c
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 72.83 cM"
     gene            1..4456
                     /gene="Sprtn"
                     /gene_synonym="Gm505"
                     /note="SprT-like N-terminal domain"
                     /db_xref="GeneID:244666"
                     /db_xref="MGI:MGI:2685351"
     exon            1..488
                     /gene="Sprtn"
                     /gene_synonym="Gm505"
                     /inference="alignment:Splign:2.1.0"
     CDS             265..1758
                     /gene="Sprtn"
                     /gene_synonym="Gm505"
                     /note="isoform 1 is encoded by transcript variant 1;
                     spartan; protein with SprT-like domain at the N terminus;
                     sprT-like domain-containing protein Spartan; DNA-dependent
                     metalloprotease SPRTN"
                     /codon_start=1
                     /product="DNA-dependent metalloprotease SPRTN isoform 1"
                     /protein_id="NP_001104611.1"
                     /db_xref="CCDS:CCDS52705.1"
                     /db_xref="GeneID:244666"
                     /db_xref="MGI:MGI:2685351"
                     /translation="MDEDLVVALRLQEEWDVQMARRAAAAREPVSLVDASWELVDPTP
                     DLQALFLQFNDRFFWGQLEAVEVKWSVRMTLCAGICTYEGRGGMCSIRLSEPLLKLRP
                     RKDLVETLLHEMIHAYLFVTNNDKDREGHGPEFCKHMHRINQLTGANITVYHTFHDEV
                     DEYRRHWWRCNGPCQHRQPYYGYVKRATNRAPSVHDYWWADHQKTCGGTYIKIKEPEN
                     YSKKGRGKTKADKQPASAVENKDKLCRGEAQLLIPFSGKGYVLGDASTCPSAGKLNTS
                     YMVNEAKGLSSQDHSVSGLRLNSNAEVKCEQNCLPKKPHLVSPLPTASHQSVLSSYFP
                     RVSVANQKAFRNVNGSPVKNGTTGDGTKRPASGGSQRKVPPSRASLRNTSKVTAPASA
                     TVTSAAGTSATISREESGSEDQFLNKRPRLEDRTALDTIKEQTQSGGDLRSSSQPTAA
                     SAPQSLSSQRRLVNCPVCQGVVVESQINEHLDRCLEGNKTNLRPRRV"
     exon            489..588
                     /gene="Sprtn"
                     /gene_synonym="Gm505"
                     /inference="alignment:Splign:2.1.0"
     exon            589..717
                     /gene="Sprtn"
                     /gene_synonym="Gm505"
                     /inference="alignment:Splign:2.1.0"
     exon            718..985
                     /gene="Sprtn"
                     /gene_synonym="Gm505"
                     /inference="alignment:Splign:2.1.0"
     exon            986..4456
                     /gene="Sprtn"
                     /gene_synonym="Gm505"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4434..4439
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sprtn"
                     /gene_synonym="Gm505"
                     /note="hexamer: AATAAA"
     polyA_site      4456
                     /gene="Sprtn"
                     /gene_synonym="Gm505"
                     /note="major polyA site"
ORIGIN      
        1 agaggagggt ccggcacgga ccagacttga cgtgtgtcgc cggacctcag cggctgtcag
       61 gagacaaccg gctgcctaaa gccgtcccca gcccgagccc agcgttccta agcctgtgcc
      121 cgcggggcag cgccgagcca ccttcggtta ggcgttcgag gctagcccgc gcgccctgac
      181 agcagggccg ggaggcctcg cgagatccgt gcgccgcacg agcccgaccg gcgtcttggg
      241 agcggccgag gtgggagtag cgggatggac gaggatctgg tggtcgcgtt gcggctgcag
      301 gaggagtggg acgtgcagat ggcgcggcgc gcggcggcgg cgcgggagcc cgtgtcgttg
      361 gtggacgcgt cctgggagct ggtggacccc accccagacc tgcaggcgct cttcctgcag
      421 tttaacgatc gcttcttctg gggtcagctg gaggcggtgg aggtgaagtg gagtgtgcgc
      481 atgaccctgt gtgctgggat ctgcacctat gaagggagag gaggcatgtg ctccatccgt
      541 ctcagtgagc ccctgttaaa actgagacca agaaaggacc ttgtagagac tcttttgcat
      601 gaaatgatac atgcctactt atttgtcact aataatgata aagaccggga aggacacggc
      661 ccagagttct gtaaacatat gcatcgaatc aaccaactga caggagccaa cataacggtc
      721 taccacactt tccatgatga ggtggatgag tatcgccggc actggtggcg ctgcaatgga
      781 ccctgccagc acagacagcc ctactacggc tatgtgaagc gtgccaccaa cagggccccc
      841 tctgttcatg actactggtg ggctgaccac cagaaaactt gtggaggcac ttacataaaa
      901 atcaaggagc cagagaacta ttcgaagaaa ggcagaggga agacaaaagc agacaagcag
      961 ccagcatcgg cagtggagaa taaagacaag ctgtgcagag gggaggccca gctgctcatc
     1021 ccattcagtg gaaaaggcta tgttcttgga gacgcaagca cttgcccttc agctgggaag
     1081 ctgaacacct catacatggt taatgaagcc aaaggtctct caagtcaaga ccattcagtg
     1141 agtggcctga ggctcaattc caatgctgag gtgaaatgtg aacagaactg tctcccaaag
     1201 aaacctcatc ttgtcagccc tcttcccact gctagtcacc aaagtgtcct gagctcctac
     1261 ttccccagag tctctgttgc caaccaaaag gctttccgaa atgtgaatgg atccccagta
     1321 aagaacggga ccactgggga tggcaccaag cgtcctgcct caggaggctc tcagaggaag
     1381 gtcccacctt ccagggcatc cctgagaaat acctccaaag tcacagcacc agcgtcagca
     1441 accgtgacat ctgcagctgg gacatccgca accatatccc gagaggagag tgggtctgaa
     1501 gaccaattcc tgaacaagcg acccaggttg gaggacagga ctgctttgga tactatcaag
     1561 gaacaaacac aaagtggtgg tgaccttcgg agtagctcac agcccacagc cgccagcgct
     1621 cctcagagct tgagcagtca acggcggctg gtcaactgtc cagtgtgtca gggcgtggtg
     1681 gtggagtcac agattaacga gcacttggac cgatgcctgg aaggtaacaa aaccaacctg
     1741 cgacctcgaa gagtgtgagg tggagtgggt cccacgctgc ccgttggctc atggtggtgt
     1801 cacctggcag gtggcaggtg agagacctgt atcttacagt ctgactgact gcgccagcgc
     1861 tttatagaca cccagtgagc aactgtagtt taagtctgca actccatagg gggggagtgt
     1921 gcctgtaata ccagatgctc aggaagctga ggcaggagca tcactccagc ccacgaccct
     1981 gaggaatgta gggagcctct gtctcaggag cttccgtgtc agtgtgctgt gtccatccac
     2041 gccttaccag gacatggctc cctttgtccc gtgaccgcgt atggctttat gtacttttgg
     2101 gtcgttttgt tttgttcatg aaggtttaga gtgcttctgt ttttatgctc agggtagtct
     2161 tatctggagt ccctgacagc tgaatactgt taacgcgaag ccccatcctc taagccgatg
     2221 ttcatcattt tgaggtctga cactttgttc agatagtatg aaactgtagt tgtcctaaaa
     2281 ggactcaaga gttctcttta aatcctaaca gctttctgta gtttaaaaga tttgactttc
     2341 tacataaagg tggcaccaaa ttttagactt ctatattctt agtaacttat attttaaata
     2401 gaatatattt atgtatataa tttgtcctac ttttactaaa aagtggcagc agattttaaa
     2461 gactcatata tttaaaagaa tttttctagt taatgataac tcatgtttaa aatcccagta
     2521 cttgggaagc tgaggcaggg agatggctga gggggtttga agccagccta ggcttaagct
     2581 ccagtgtggg ctatgatgag accatgtctc aaagtttttg tttgggcttt tttcctcctt
     2641 ccttccttcc ttccctccct ccgtccctcc ctccctccct tccttccttc cttccttcct
     2701 tttttcccct ctgtcccatc tttatttacc aagcagcggt tgcagaattg agagtaaagt
     2761 ggataccgaa tgtacagtgc agttgttttg gaatgttaaa gtggttactt ggttctgcag
     2821 gccagatttg atgggaacgt aagtgaatgg tttaaacttg gtgctaaaat gtaactgatg
     2881 tataacatct caccaagctg cctgattcct tggagagcag aaaatgccta ggcagcactg
     2941 tgaactctac accaaagtgc gagcaaagat gaccatctga gctctggaag gttgtgtctg
     3001 tctgcatccg ggcaggggac gcagacatgt ggctcagttt atcttgtctc ctgtctgttc
     3061 ctccaccttg ctgactttgc tacacggtgc ataaatcttg tgcattttgg ggtgaacctg
     3121 gtttgtccgg tgttcccctg tataatttcc tccattacgt cccccagggg agctggggga
     3181 gtcctgctgt agttgaccct tcacagggcc tgccttctcc atccccagag gtcacagttt
     3241 gttaccagag agtgtgaagg tagtttcttt gttaccgtgt aggcttattt tcattccttt
     3301 gtagaattta aagatagcta gaagtttcct ccctcacccc agtattattg aaccattgga
     3361 agtgtcccag tgcagggcta agcaatgctg acaacagcca ctcaggagtg gacgtcacag
     3421 ggtgtctgtg gtcagctcta tccaactctc ctcccagcag tggccactgt ggcctttcag
     3481 catccctgac agggtcttta ggtgcaggat tgggtgacaa gttttgctat ttcttaaagc
     3541 atgaagttaa tagtatttaa tgtttatagg taaatagcta ttaaaggttt ttttggatat
     3601 atgccatatt ttcactaatt taacaggtta tagacaatta aatatttttg tgccaaacta
     3661 cctaagttct agggctatga aacaaattag gtggtagttc acacctttca ggagctacat
     3721 cctcagtgag ggaaacctgc agattaatat tggtgctttg tgatggtgct gggtcagggt
     3781 tttactctgg cactggtttt gagttgagtt agcttgttaa ggaaggggaa gggcctcagg
     3841 ctgggagaga actgggtaca tcctgtagaa acacaagatg gtgagctgga tggaatgtga
     3901 gatttgagca gagggcagga gggagggaga gtgtagacag gttgagggtg ggactgcacc
     3961 tgctgtgtct gtctactaac cacctgtgca gagtagccag gtgcagtctc ggcaacacca
     4021 gcttctcagc atggtgagaa tgctagaaag tgggaatgta atgagcctgt agttgttacg
     4081 aagatttgcc cctaagagta gttacccttt tatctgtcat tttatcagtg aaatgtggat
     4141 tttgtttgta ttttcccact ggaaggaaat ttctaacctc acagtggagc tgaagttggt
     4201 tcagctccac actttagggc cacagctgta ctcctgtttt acagaatttg tgcttacatc
     4261 ccagttccat gctagtaaaa agcagtgtga gaaacctttc cttggctttg cccaactgag
     4321 tccacatcct aactgggacc tctgaagaaa agggaaaaac attaaaaccc cattctgtct
     4381 tgttccaaag ctctaagatt gtattttttt ttcaaagctt tacagaattt aaaaataaac
     4441 agtgttttga tgtaaa
//
