LOCUS       NM_183147               3172 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus shadow of prion protein (Sprn), mRNA.
ACCESSION   NM_183147
VERSION     NM_183147.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Castille,J., Passet,B., Makhzami,S., Vilotte,M.,
            Moazami-Goudarzi,K., Truchet,S., Daniel-Carlier,N., Gaillard,A.L.,
            Andreoletti,O., Vaiman,D., Beauvallet,C., Vaiman,A., Floriot,S.,
            Calvel,P., Mouillet-Richard,S., Duchesne,A., Beringue,V. and
            Vilotte,J.L.
  TITLE     Co-invalidation of Prnp and Sprn in FVB/N mice affects reproductive
            performances and highlight complex biological relationship between
            PrP and Shadoo
  JOURNAL   Biochem Biophys Res Commun 551, 1-6 (2021)
   PUBMED   33713980
  REMARK    GeneRIF: Co-invalidation of Prnp and Sprn in FVB/N mice affects
            reproductive performances and highlight complex biological
            relationship between PrP and Shadoo.
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Passet,B., Castille,J., Makhzami,S., Truchet,S., Vaiman,A.,
            Floriot,S., Moazami-Goudarzi,K., Vilotte,M., Gaillard,A.L.,
            Helary,L., Bertaud,M., Andreoletti,O., Vaiman,D., Calvel,P.,
            Daniel-Carlier,N., Moudjou,M., Beauvallet,C., Benharouga,M.,
            Laloe,D., Mouillet-Richard,S., Duchesne,A., Beringue,V. and
            Vilotte,J.L.
  TITLE     The Prion-like protein Shadoo is involved in mouse embryonic and
            mammary development and differentiation
  JOURNAL   Sci Rep 10 (1), 6765 (2020)
   PUBMED   32317725
  REMARK    GeneRIF: The Prion-like protein Shadoo is involved in mouse
            embryonic and mammary development and differentiation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3172)
  AUTHORS   Kang,S.G., Mays,C.E., Daude,N., Yang,J., Kar,S. and Westaway,D.
  TITLE     Proteasomal Inhibition Redirects the PrP-Like Shadoo Protein to the
            Nucleus
  JOURNAL   Mol Neurobiol 56 (11), 7888-7904 (2019)
   PUBMED   31129810
  REMARK    GeneRIF: These data are indicative of a physiological pathway to
            access Sho functions in the nucleus under conditions of impaired
            proteasomal activity.
REFERENCE   4  (bases 1 to 3172)
  AUTHORS   Nyeste,A., Bencsura,P., Vida,I., Hegyi,Z., Homolya,L., Fodor,E. and
            Welker,E.
  TITLE     Expression of the Prion Protein Family Member Shadoo Causes Drug
            Hypersensitivity That Is Diminished by the Coexpression of the Wild
            Type Prion Protein
  JOURNAL   J Biol Chem 291 (9), 4473-4486 (2016)
   PUBMED   26721882
  REMARK    GeneRIF: Data suggest that expression of shadoo in neuron or
            hepatocyte cell lines induces same toxic phenotype of drug
            hypersensitivity as prion protein/PrP(-central region); this effect
            is counteracted by co-expression of PrP-wild-type.
REFERENCE   5  (bases 1 to 3172)
  AUTHORS   Ciric,D., Richard,C.A., Moudjou,M., Chapuis,J., Sibille,P.,
            Daude,N., Westaway,D., Adrover,M., Beringue,V., Martin,D. and
            Rezaei,H.
  TITLE     Interaction between Shadoo and PrP Affects the PrP-Folding Pathway
  JOURNAL   J Virol 89 (12), 6287-6293 (2015)
   PUBMED   25855735
  REMARK    GeneRIF: These observations suggest that Shadoo can affect the
            prion replication process by (i) acting as a holdase and (ii)
            interfering with the dominant-negative inhibitor effect of the C1
            fragment.
REFERENCE   6  (bases 1 to 3172)
  AUTHORS   Jiayu,W., Zhu,H., Ming,X., Xiong,W., Songbo,W., Bocui,S.,
            Wensen,L., Jiping,L., Keying,M., Zhongyi,L. and Hongwei,G.
  TITLE     Mapping the interaction site of prion protein and Sho
  JOURNAL   Mol Biol Rep 37 (5), 2295-2300 (2010)
   PUBMED   19685161
  REMARK    GeneRIF: Sho may play a role in the physiological function of
            PrP(C) and prion pathogenesis.
REFERENCE   7  (bases 1 to 3172)
  AUTHORS   Lloyd,S.E., Grizenkova,J., Pota,H. and Collinge,J.
  TITLE     Shadoo (Sprn) and prion disease incubation time in mice
  JOURNAL   Mamm Genome 20 (6), 367-374 (2009)
   PUBMED   19513788
  REMARK    GeneRIF: Sprn does not play a major role in prion disease
            incubation time in mice.
REFERENCE   8  (bases 1 to 3172)
  AUTHORS   Watts,J.C., Drisaldi,B., Ng,V., Yang,J., Strome,B., Horne,P.,
            Sy,M.S., Yoong,L., Young,R., Mastrangelo,P., Bergeron,C.,
            Fraser,P.E., Carlson,G.A., Mount,H.T., Schmitt-Ulms,G. and
            Westaway,D.
  TITLE     The CNS glycoprotein Shadoo has PrP(C)-like protective properties
            and displays reduced levels in prion infections
  JOURNAL   EMBO J 26 (17), 4038-4050 (2007)
   PUBMED   17703189
  REMARK    GeneRIF: Sho is a candidate for pi, and since it engenders a
            PrP(C)-like neuroprotective activity, compromised neuroprotective
            activity resulting from reduced levels exacerbate damage in prion
            infections.
REFERENCE   9  (bases 1 to 3172)
  AUTHORS   Premzl,M., Gready,J.E., Jermiin,L.S., Simonic,T. and Marshall
            Graves,J.A.
  TITLE     Evolution of vertebrate genes related to prion and Shadoo
            proteins--clues from comparative genomic analysis
  JOURNAL   Mol Biol Evol 21 (12), 2210-2231 (2004)
   PUBMED   15342797
REFERENCE   10 (bases 1 to 3172)
  AUTHORS   Premzl,M., Sangiorgio,L., Strumbo,B., Marshall Graves,J.A.,
            Simonic,T. and Gready,J.E.
  TITLE     Shadoo, a new protein highly conserved from fish to mammals and
            with similarity to prion protein
  JOURNAL   Gene 314, 89-102 (2003)
   PUBMED   14527721
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC056484.1.
            
            On Nov 29, 2004 this sequence version replaced NM_183147.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC056484.1, SRR1660815.203964.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 85.17 cM"
     gene            1..3172
                     /gene="Sprn"
                     /gene_synonym="C630041J07"
                     /note="shadow of prion protein"
                     /db_xref="GeneID:212518"
                     /db_xref="MGI:MGI:3582583"
     exon            1..111
                     /gene="Sprn"
                     /gene_synonym="C630041J07"
                     /inference="alignment:Splign:2.1.0"
     exon            112..3156
                     /gene="Sprn"
                     /gene_synonym="C630041J07"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..570
                     /gene="Sprn"
                     /gene_synonym="C630041J07"
                     /note="Shadoo; protein shadoo"
                     /codon_start=1
                     /product="shadow of prion protein precursor"
                     /protein_id="NP_898970.1"
                     /db_xref="CCDS:CCDS21966.1"
                     /db_xref="GeneID:212518"
                     /db_xref="MGI:MGI:3582583"
                     /translation="MNWTAATCWALLLAAAFLCDSCSAKGGRGGARGSARGVRGGARG
                     ASRVRVRPAPRYGSSLRVAAAGAAAGAAAGVAAGLATGSGWRRTSGPGELGLEDDENG
                     AMGGNGTDRGVYSYWAWTSGSGSVHSPRICLLLGGTLGALELLRP"
     regulatory      3133..3138
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sprn"
                     /gene_synonym="C630041J07"
                     /note="hexamer: AATAAA"
     regulatory      3137..3142
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sprn"
                     /gene_synonym="C630041J07"
                     /note="hexamer: AATAAA"
     polyA_site      3142
                     /gene="Sprn"
                     /gene_synonym="C630041J07"
                     /note="major polyA site"
     polyA_site      3153
                     /gene="Sprn"
                     /gene_synonym="C630041J07"
     polyA_site      3156
                     /gene="Sprn"
                     /gene_synonym="C630041J07"
ORIGIN      
        1 agcaaaatcc agtcgtgagc tctgcctaag gaaaagggtc ctcgctgccg cacctttccg
       61 cttggccggt cacggcccct aaatcgctca tccgacctag gcttgtgacc aattctgccc
      121 agtaggatga actggactgc tgccacgtgc tgggctctgc tgctggccgc cgccttcctc
      181 tgtgacagct gttccgccaa gggcggccgc ggaggcgctc gaggcagtgc ccggggggtg
      241 cgcggaggtg cacgcggggc atcaagagta cgcgtaaggc cggcgccccg ctacggctcc
      301 tctctgcgcg tggcggctgc aggggcagca gcaggggctg cagcgggtgt ggctgcgggc
      361 cttgctaccg gctctggctg gaggaggacc tcagggcctg gagagctagg cctggaggac
      421 gatgagaatg gggcaatggg aggcaacgga accgaccgag gagtctacag ctactgggcc
      481 tggacttccg gctcagggtc cgtgcacagc ccacgtattt gtctgcttct gggcggcacc
      541 cttggtgccc tagaactgct tcggccttag accaggctaa actccatccc aggtctagct
      601 cctagcctgt cttaaggccc ctagggccca ccctaatggc ctcctgccca gggggtaact
      661 tcattttgct aactatgatt ccccagccca gagaagagtc tggccattct gggcccacaa
      721 ggcctcttgc aatttgtgga tgtagtttac tctcttgccc atcccagttt cccagtcttc
      781 cactctggca ggaactaaca gccctactac cagagaggca ggctgccctt gactctccag
      841 aactgcccaa gcaggcatgc ctgtctgctt gcccaccctg acacgaggac atgaacaatg
      901 ctgaccgcca agaagaggcc atccttgggt gggcccgtct gattctgcca ctaaagcccc
      961 caaagacctt gagcctccca tggaaccaga gggacttata gttgatgaag agagcattca
     1021 cttagactgc agtgtgagaa atgtcaggtg ctaaccacct gcccagagca ggtcccaatg
     1081 atgtcatagc tcagaaaaca tccagagcag taaaataacc atcccaggac gcccaccatg
     1141 ctcctcctac aacatgctac caaagccaag tagtatgttt cttcctggca gactgcctaa
     1201 gagaccttgt gtcagaaggg tttccacttg aagccacttg gtcctaaaat ccactgaggg
     1261 tagaggtttt gaatacactt tcaaaaacat tccattctgc ttgagcttaa gggtcatgag
     1321 tgagggtcac ttggatataa taccaatcct gctggggcct tctttgtata taacccaaac
     1381 tgcaagattc ccatagttcc agtagatagc agcattttat gttgggagac ccctcccttg
     1441 gaaacggttg gacggggttg gggtggggag gtgagacaga gcatggcaca gctgacagct
     1501 ggcaaactga acagtggaag gggcagcaga tctacagccc cactgtgcca gaacaaagct
     1561 agcagacaga agaaccagcc tcagcaggtt gtgagcgtgg gttaatgcta ctagctctct
     1621 ctgaccatcc atatacatcc tgagaacaga gctgatgtgc tgagtggcct tagagaggta
     1681 ctctataatc ctcacaagaa tctaccttta agcagactgg cttcttgctg ttccagggac
     1741 ccactagcac atctttgcca catggagtgt tgggtcttgg gattcagtca atatatgcta
     1801 gctcagcata cctctggcta gtgtataggt ttcaaagcta ttggcagggc cctagccaag
     1861 cattttcttg accaacaggt tgcaggatac aactccctgc aacttctatt ctcactgccg
     1921 gtgctcacag gcacagtcag cccagatgct cctatgtctc taatagacca agctcaaaac
     1981 caccaagacc agcaagctaa gggccaagcc cactaaggtc ctcaaaggca ggtgctgatg
     2041 cgtcaagtct ggtataggga gaaccaccag agccccttca acttgtccaa cacccatgag
     2101 gatggactat gtggaccaga cctttgtgta ccacagagta ccatgtgtta gttctgggta
     2161 ttgtggcatt atgaacacat cacctttcga tgattatttt acattcacca atctgacttc
     2221 tccagcgtgt ttagcatctg gcacagccac acatctttaa ataccttctc cactagcagg
     2281 aactatagat gctgagggag aatctcagaa gagacttata atactgcagc cacagcaaaa
     2341 ggcaacagtg ggaggctacc cctgctatca gccatccaca agaagcagag agcctattga
     2401 gcatccaagt actggttcaa gcaactatgg aaagctcagg tcaataccga acagaagaga
     2461 ctaaaactcc tgagcaggga tagccccctg gcccaacagc acttcctatc tgtatcgacc
     2521 aaccccttgc cttttctttt tagacagcaa gctgctggca gcaacatctt ctaggtgcct
     2581 acaggtattt ggaggtcaac catgctggta catgccctct gcaccgcccc tgcagccacc
     2641 atggtttcaa tggctagact gtgagcccct tccagtgatc ctcttggcct gtacaaagtt
     2701 gagctacagc agcaggcctc ctgaggtaca ggccctgaag tgcaagggga gatgacattc
     2761 ctggacttct ctgctgcaga cctcctgtcc gtgtctgtgc actttatcag tgaagagttc
     2821 cttgtgaaat aaagccctct gctggtaaca ggcttttccc tcattgcagg actttgagac
     2881 acttgagaat ctgacttagg aaccccaggc ctctaatcta ccactcctca gcattcattg
     2941 atagaaggag atttaactta tgggctcacc atactaagtg cacaatgaaa acgccatcag
     3001 aaaataggtc ttttaatcgg ttgtcatggc acacacctgt ataattccag cactcaagag
     3061 gctaaggcag aaggaatgtg tttgagacca gcccaagcca catgatgaaa ctttgtctga
     3121 aaatgacaaa aaaataaata aagtaaaata agagaaaaaa aaaaaaaaaa aa
//
